P46409 (GSTMU_RABIT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Glutathione S-transferase Mu 1 EC=2.5.1.18 Alternative name(s): GST Mu I GST class-mu
Organism	Oryctolagus cuniculus (Rabbit) [Reference proteome]
Taxonomic identifier	9986 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	218 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm.
Tissue specificity	Well expressed in rabbit liver, brain and kidney.
Sequence similarities	Belongs to the GST superfamily. Mu family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 218	217	Glutathione S-transferase Mu 1		PRO_0000185837
Regions
	Domain	2 – 88	87	GST N-terminal
	Domain	90 – 208	119	GST C-terminal
	Region	7 – 8	2	Glutathione binding By similarity
	Region	46 – 50	5	Glutathione binding By similarity
	Region	59 – 60	2	Glutathione binding By similarity
	Region	72 – 73	2	Glutathione binding By similarity
Sites
	Binding site	116	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P46409 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 481EA4AB85671102	FASTA	218	25,417
	10 20 30 40 50 60 MPMTLGYWDV RGLALPIRML LEYTDTSYEE KKYTMGDAPN YDQSKWLSEK FTLGLDFPNL 70 80 90 100 110 120 PYLIDGTHKL TQSNAILRYL ARKHGLCGET EEERIRVDIL ENQLMDNRFQ LVNVCYSPDF 130 140 150 160 170 180 EKLKPEYLKG LPEKLQLYSQ FLGSLPWFAG DKITFADFLV YDVLDQNRIF VPGCLDAFPN 190 200 210 LKDFHVRFEG LPKISAYMKS SRFIRVPVFL KKATWTGI

References

[1]	"Cloning and expression of a cDNA for mu-class glutathione S-transferase from rabbit liver." Lee S.H., Lee S.H., Han J.S., Kim Y.S., Koh J.K. Arch. Biochem. Biophys. 318:424-429(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"Purification and characterization of class mu glutathione S-transferase isozymes from rabbit hepatic tissue." Primiano T., Novak R.F. Arch. Biochem. Biophys. 301:404-410(1993) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-21. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L23766 mRNA. Translation: AAA69665.1.
PIR	S65674.
RefSeq	NP_001075721.1. NM_001082252.1.
UniGene	Ocu.2029.
3D structure databases
ProteinModelPortal	P46409.
SMR	P46409. Positions 1-215.
ModBase	Search...
Protein-protein interaction databases
STRING	9986.ENSOCUP00000021965.
Proteomic databases
PRIDE	P46409.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100009073.
Organism-specific databases
CTD	2946.
Phylogenomic databases
eggNOG	NOG300089.
HOGENOM	HOG000115735.
HOVERGEN	HBG106842.
OrthoDB	EOG47D9H2.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR003081. GST_mu. IPR012336. Thioredoxin-like_fold. [Graphical view]
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
PRINTS	PR01267. GSTRNSFRASEM.
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSTMU_RABIT

Accession

Primary (citable) accession number: P46409

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program