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P46396

- BIOB_CORGL

UniProt

P46396 - BIOB_CORGL

Protein

Biotin synthase

Gene

bioB

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 3 (11 Jul 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi70 – 701Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi74 – 741Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi77 – 771Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi113 – 1131Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi205 – 2051Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi275 – 2751Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:Cgl0072, cg0095
    OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
    Taxonomic identifieri196627 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
    ProteomesiUP000000582: Chromosome, UP000001009: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 334334Biotin synthasePRO_0000185551Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi196627.cg0095.

    Structurei

    3D structure databases

    ProteinModelPortaliP46396.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239958.
    KOiK01012.
    OMAiTCENTLR.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P46396-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTIPGTILDT ARTQVLEQGI GLNQQQLMEV LTLPEEQIPD LMELAHQVRL    50
    KWCGEEIEVE GIISLKTGGC PEDCHFCSQS GLFESPVRSV WLDIPNLVEA 100
    AKQTAKTGAT EFCIVAAVKG PDERLMTQLE EAVLAIHSEV EIEVAASIGT 150
    LNKEQVDRLA AAGVHRYNHN LETARSYFPE VVTTHTWEER RETLRLVAEA 200
    GMEVCSGGIL GMGETLEQRA EFAVQLAELD PHEVPMNFLD PRPGTPFADR 250
    ELMDSRDALR SIGAFRLAMP HTMLRFAGGR ELTLGDKGSE QALLGGINAM 300
    IVGNYLTTLG RPMEDDLDMM DRLQLPIKVL NKVI 334
    Length:334
    Mass (Da):36,856
    Last modified:July 11, 2002 - v3
    Checksum:iE0EF4E812C6BF096
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti5 – 51G → A(PubMed:8173080)Curated
    Sequence conflicti5 – 51G → A(PubMed:8917070)Curated
    Sequence conflicti88 – 881R → A in BAA03169. (PubMed:8173080)Curated
    Sequence conflicti113 – 1142CI → DF in BAA03169. (PubMed:8173080)Curated
    Sequence conflicti232 – 2321H → D(PubMed:8173080)Curated
    Sequence conflicti232 – 2321H → D(PubMed:8917070)Curated
    Sequence conflicti260 – 2601R → A in BAA03169. (PubMed:8173080)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D14084 Genomic DNA. Translation: BAA03169.1.
    U31281 Genomic DNA. Translation: AAC44580.1.
    BA000036 Genomic DNA. Translation: BAB97465.1.
    BX927148 Genomic DNA. Translation: CAF18640.1.
    PIRiI40338.
    JC5084.
    RefSeqiNP_599324.1. NC_003450.3.
    YP_224369.1. NC_006958.1.

    Genome annotation databases

    EnsemblBacteriaiBAB97465; BAB97465; BAB97465.
    CAF18640; CAF18640; cg0095.
    GeneIDi1021113.
    KEGGicgb:cg0095.
    cgl:NCgl0071.
    PATRICi21492182. VBICorGlu203724_0073.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D14084 Genomic DNA. Translation: BAA03169.1 .
    U31281 Genomic DNA. Translation: AAC44580.1 .
    BA000036 Genomic DNA. Translation: BAB97465.1 .
    BX927148 Genomic DNA. Translation: CAF18640.1 .
    PIRi I40338.
    JC5084.
    RefSeqi NP_599324.1. NC_003450.3.
    YP_224369.1. NC_006958.1.

    3D structure databases

    ProteinModelPortali P46396.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 196627.cg0095.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB97465 ; BAB97465 ; BAB97465 .
    CAF18640 ; CAF18640 ; cg0095 .
    GeneIDi 1021113.
    KEGGi cgb:cg0095.
    cgl:NCgl0071.
    PATRICi 21492182. VBICorGlu203724_0073.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239958.
    KOi K01012.
    OMAi TCENTLR.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of the biotin biosynthesis pathway in coryneform bacteria: cloning and sequencing of the bioB gene from Brevibacterium flavum."
      Hatakeyama K., Kohama K., Vertes A.A., Kobayashi M., Kurusu Y., Yukawa H.
      DNA Seq. 4:87-93(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: MJ233.
    2. "Two new members of the bio B superfamily: cloning, sequencing and expression of bio B genes of Methylobacillus flagellatum and Corynebacterium glutamicum."
      Serebriiskii I.G., Vassin V.M., Tsygankov Y.D.
      Gene 175:15-22(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
    3. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
      Ikeda M., Nakagawa S.
      Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

    Entry informationi

    Entry nameiBIOB_CORGL
    AccessioniPrimary (citable) accession number: P46396
    Secondary accession number(s): P94636
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: July 11, 2002
    Last modified: October 1, 2014
    This is version 109 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3