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P46396 (BIOB_CORGL) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:Cgl0072, cg0095
OrganismCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025) [Reference proteome] [HAMAP]
Taxonomic identifier196627 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium

Protein attributes

Sequence length334 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 334334Biotin synthase HAMAP-Rule MF_01694
PRO_0000185551

Sites

Metal binding701Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding741Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding771Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1131Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2051Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2751Iron-sulfur 2 (2Fe-2S) By similarity

Experimental info

Sequence conflict51G → A Ref.1
Sequence conflict51G → A Ref.2
Sequence conflict881R → A in BAA03169. Ref.1
Sequence conflict113 – 1142CI → DF in BAA03169. Ref.1
Sequence conflict2321H → D Ref.1
Sequence conflict2321H → D Ref.2
Sequence conflict2601R → A in BAA03169. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P46396 [UniParc].

Last modified July 11, 2002. Version 3.
Checksum: E0EF4E812C6BF096

FASTA33436,856
        10         20         30         40         50         60 
MTIPGTILDT ARTQVLEQGI GLNQQQLMEV LTLPEEQIPD LMELAHQVRL KWCGEEIEVE 

        70         80         90        100        110        120 
GIISLKTGGC PEDCHFCSQS GLFESPVRSV WLDIPNLVEA AKQTAKTGAT EFCIVAAVKG 

       130        140        150        160        170        180 
PDERLMTQLE EAVLAIHSEV EIEVAASIGT LNKEQVDRLA AAGVHRYNHN LETARSYFPE 

       190        200        210        220        230        240 
VVTTHTWEER RETLRLVAEA GMEVCSGGIL GMGETLEQRA EFAVQLAELD PHEVPMNFLD 

       250        260        270        280        290        300 
PRPGTPFADR ELMDSRDALR SIGAFRLAMP HTMLRFAGGR ELTLGDKGSE QALLGGINAM 

       310        320        330 
IVGNYLTTLG RPMEDDLDMM DRLQLPIKVL NKVI 

« Hide

References

« Hide 'large scale' references
[1]"Analysis of the biotin biosynthesis pathway in coryneform bacteria: cloning and sequencing of the bioB gene from Brevibacterium flavum."
Hatakeyama K., Kohama K., Vertes A.A., Kobayashi M., Kurusu Y., Yukawa H.
DNA Seq. 4:87-93(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: MJ233.
[2]"Two new members of the bio B superfamily: cloning, sequencing and expression of bio B genes of Methylobacillus flagellatum and Corynebacterium glutamicum."
Serebriiskii I.G., Vassin V.M., Tsygankov Y.D.
Gene 175:15-22(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[3]"The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
Ikeda M., Nakagawa S.
Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[4]"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins."
Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. expand/collapse author list , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A.
J. Biotechnol. 104:5-25(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D14084 Genomic DNA. Translation: BAA03169.1.
U31281 Genomic DNA. Translation: AAC44580.1.
BA000036 Genomic DNA. Translation: BAB97465.1.
BX927148 Genomic DNA. Translation: CAF18640.1.
PIRI40338.
JC5084.
RefSeqNP_599324.1. NC_003450.3.
YP_224369.1. NC_006958.1.

3D structure databases

ProteinModelPortalP46396.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING196627.cg0095.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB97465; BAB97465; BAB97465.
CAF18640; CAF18640; cg0095.
GeneID1021113.
KEGGcgb:cg0095.
cgl:NCgl0071.
PATRIC21492182. VBICorGlu203724_0073.

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239958.
KOK01012.
OMATCENTLR.
OrthoDBEOG622PMP.

Enzyme and pathway databases

UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_CORGL
AccessionPrimary (citable) accession number: P46396
Secondary accession number(s): P94636
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: July 11, 2002
Last modified: July 9, 2014
This is version 108 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways