P46396 (BIOB_CORGL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Biotin synthase
EC=2.8.1.6

Gene names

Name:	bioB
Ordered Locus Names:	Cgl0072, cg0095

Organism

Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025) [Reference proteome] [HAMAP]

Taxonomic identifier

196627 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium › Corynebacterium glutamicum

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694
Catalytic activity	Dethiobiotin + sulfur + 2 S-adenosyl-L-methionine = biotin + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694
Cofactor	Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity. Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the radical SAM superfamily. Biotin synthase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Ligand	2Fe-2S 4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	biotin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW biotin synthase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 334	334	Biotin synthase HAMAP-Rule MF_01694		PRO_0000185551
Sites
	Metal binding	70	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	74	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	77	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	113	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	205	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	275	1	Iron-sulfur 2 (2Fe-2S) By similarity
Experimental info
	Sequence conflict	5	1	G → A Ref.1
	Sequence conflict	5	1	G → A Ref.2
	Sequence conflict	88	1	R → A in BAA03169. Ref.1
	Sequence conflict	113 – 114	2	CI → DF in BAA03169. Ref.1
	Sequence conflict	232	1	H → D Ref.1
	Sequence conflict	232	1	H → D Ref.2
	Sequence conflict	260	1	R → A in BAA03169. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P46396 [UniParc]. Last modified July 11, 2002. Version 3. Checksum: E0EF4E812C6BF096	FASTA	334	36,856
	10 20 30 40 50 60 MTIPGTILDT ARTQVLEQGI GLNQQQLMEV LTLPEEQIPD LMELAHQVRL KWCGEEIEVE 70 80 90 100 110 120 GIISLKTGGC PEDCHFCSQS GLFESPVRSV WLDIPNLVEA AKQTAKTGAT EFCIVAAVKG 130 140 150 160 170 180 PDERLMTQLE EAVLAIHSEV EIEVAASIGT LNKEQVDRLA AAGVHRYNHN LETARSYFPE 190 200 210 220 230 240 VVTTHTWEER RETLRLVAEA GMEVCSGGIL GMGETLEQRA EFAVQLAELD PHEVPMNFLD 250 260 270 280 290 300 PRPGTPFADR ELMDSRDALR SIGAFRLAMP HTMLRFAGGR ELTLGDKGSE QALLGGINAM 310 320 330 IVGNYLTTLG RPMEDDLDMM DRLQLPIKVL NKVI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the biotin biosynthesis pathway in coryneform bacteria: cloning and sequencing of the bioB gene from Brevibacterium flavum." Hatakeyama K., Kohama K., Vertes A.A., Kobayashi M., Kurusu Y., Yukawa H. DNA Seq. 4:87-93(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: MJ233.
[2]	"Two new members of the bio B superfamily: cloning, sequencing and expression of bio B genes of Methylobacillus flagellatum and Corynebacterium glutamicum." Serebriiskii I.G., Vassin V.M., Tsygankov Y.D. Gene 175:15-22(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[3]	"The Corynebacterium glutamicum genome: features and impacts on biotechnological processes." Ikeda M., Nakagawa S. Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[4]	"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D14084 Genomic DNA. Translation: BAA03169.1. U31281 Genomic DNA. Translation: AAC44580.1. BA000036 Genomic DNA. Translation: BAB97465.1. BX927148 Genomic DNA. Translation: CAF18640.1.
PIR	I40338. JC5084.
RefSeq	NP_599324.1. NC_003450.3. YP_224369.1. NC_006958.1.
3D structure databases
ProteinModelPortal	P46396.
ModBase	Search...
Protein-protein interaction databases
STRING	196627.cg0095.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB97465; BAB97465; BAB97465. CAF18640; CAF18640; cg0095.
GeneID	1021113. 3343513.
KEGG	cgb:cg0095. cgl:NCgl0071.
PATRIC	21492182. VBICorGlu203724_0073.
Phylogenomic databases
eggNOG	COG0502.
HOGENOM	HOG000239958.
KO	K01012.
OMA	RIMMPAS.
ProtClustDB	PRK06256.
Enzyme and pathway databases
BioCyc	CGLU196627:GJDM-77-MONOMER.
UniPathway	UPA00078; UER00162.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_01694. BioB.
InterPro	IPR013785. Aldolase_TIM. IPR010722. Biotin/thiamin_synth-assoc. IPR002684. Biotin_synth/BioAB. IPR024177. Biotin_synthase. IPR006638. Elp3/MiaB/NifB. IPR007197. rSAM. [Graphical view]
Pfam	PF06968. BATS. 1 hit. PF04055. Radical_SAM. 1 hit. [Graphical view]
PIRSF	PIRSF001619. Biotin_synth. 1 hit.
SMART	SM00876. BATS. 1 hit. SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00433. bioB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOB_CORGL

Accession

Primary (citable) accession number: P46396
Secondary accession number(s): P94636

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	July 11, 2002
Last modified:	May 1, 2013
This is version 100 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program