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P46395

- BIOA_CORGL

UniProt

P46395 - BIOA_CORGL

Protein

Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene

bioA

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 2 (13 Aug 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the transfer of the alpha-amino group from S-adenosyl-L-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form 7,8-diaminopelargonic acid (DAPA). It is the only animotransferase known to utilize SAM as an amino donor.1 PublicationUniRule annotation

    Catalytic activityi

    S-adenosyl-L-methionine + 8-amino-7-oxononanoate = S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate.UniRule annotation

    Cofactori

    Pyridoxal phosphate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei18 – 181Participates in the substrate recognition with KAPA and in a stacking interaction with the adenine ring of SAMUniRule annotation
    Binding sitei51 – 511SubstrateUniRule annotation
    Binding sitei144 – 1441SubstrateUniRule annotation
    Binding sitei243 – 2431Pyridoxal phosphateUniRule annotation
    Binding sitei272 – 2721SubstrateUniRule annotation
    Binding sitei306 – 3061Substrate; via carbonyl oxygenUniRule annotation
    Binding sitei390 – 3901SubstrateUniRule annotation

    GO - Molecular functioni

    1. adenosylmethionine-8-amino-7-oxononanoate transaminase activity Source: UniProtKB-HAMAP
    2. pyridoxal phosphate binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminotransferase, Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    Pyridoxal phosphate, S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00078; UER00160.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Adenosylmethionine-8-amino-7-oxononanoate aminotransferaseUniRule annotation (EC:2.6.1.62UniRule annotation)
    Alternative name(s):
    7,8-diamino-pelargonic acid aminotransferaseUniRule annotation
    Short name:
    DAPA ATUniRule annotation
    Short name:
    DAPA aminotransferaseUniRule annotation
    7,8-diaminononanoate synthaseUniRule annotation
    Short name:
    DANSUniRule annotation
    Diaminopelargonic acid synthaseUniRule annotation
    Gene namesi
    Name:bioAUniRule annotation
    Ordered Locus Names:Cgl2604, cg2885
    OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
    Taxonomic identifieri196627 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
    ProteomesiUP000000582: Chromosome, UP000001009: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 423423Adenosylmethionine-8-amino-7-oxononanoate aminotransferasePRO_0000120365Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei272 – 2721N6-(pyridoxal phosphate)lysineUniRule annotation

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi196627.cg2885.

    Structurei

    3D structure databases

    ProteinModelPortaliP46395.
    SMRiP46395. Positions 1-421.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni111 – 1122Pyridoxal phosphate bindingUniRule annotation
    Regioni307 – 3082Pyridoxal phosphate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. BioA subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0161.
    HOGENOMiHOG000020209.
    KOiK00833.
    OMAiFTTPDED.
    OrthoDBiEOG6QVRHN.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 2 hits.
    HAMAPiMF_00834. BioA.
    InterProiIPR005814. Aminotrans_3.
    IPR005815. BioA.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PANTHERiPTHR11986. PTHR11986. 1 hit.
    PfamiPF00202. Aminotran_3. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000521. Transaminase_4ab_Lys_Orn. 1 hit.
    SUPFAMiSSF53383. SSF53383. 1 hit.
    TIGRFAMsiTIGR00508. bioA. 1 hit.
    PROSITEiPS00600. AA_TRANSFER_CLASS_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P46395-1 [UniParc]FASTAAdd to Basket

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    MENPSLRELD HRNIWHPYAA PGVRNRLVTK TDGVFLTLED GSTVIDAMSS    50
    WWSAIHGHGH PRLKAAAQKQ IDTMSHVMFG GLTHEPAIKL THKLLNLTGN 100
    SFDHVFYSDS GSVSVEVAIK MALQASKGQG HPERTKLLTW RSGYHGDTFT 150
    AMSVCDPENG MHSLWKGTLP EQIFAPAPPV RGSSPQAISE YLRSMELLID 200
    ETVSAIIIEP IVQGAGGMRF HDVALIEGVA TLCKKHDRFL IVDEIATGFG 250
    RTGELFATLS NGLQPDIMCV GKALTGGFMS FAATLCTDKV AQLISTPNGG 300
    GALMHGPTFM ANPLACAVSH ASLEIIETGM WQKQVKRIEA ELIAGLSPLQ 350
    HLPGVADVRV LGAIGVIEME QNVNVEEATQ AALDHGVWIR PFGRLLYVMP 400
    PYITTSEQCA QICTALHAAV KGK 423
    Length:423
    Mass (Da):45,741
    Last modified:August 13, 2002 - v2
    Checksum:i88773BED8418632D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti30 – 301K → N in BAA03167. (PubMed:8161820)Curated
    Sequence conflicti65 – 651A → R in BAA03167. (PubMed:8161820)Curated
    Sequence conflicti101 – 1011S → A in BAA03167. (PubMed:8161820)Curated
    Sequence conflicti193 – 1931R → H in BAA03167. (PubMed:8161820)Curated
    Sequence conflicti231 – 2311T → A in BAA03167. (PubMed:8161820)Curated
    Sequence conflicti263 – 2631L → V in BAA03167. (PubMed:8161820)Curated
    Sequence conflicti285 – 2851L → V in BAA03167. (PubMed:8161820)Curated
    Sequence conflicti295 – 2984STPN → RSPE in BAA03167. (PubMed:8161820)Curated
    Sequence conflicti302 – 3021A → V in BAA03167. (PubMed:8161820)Curated
    Sequence conflicti317 – 3171A → E in BAA03167. (PubMed:8161820)Curated
    Sequence conflicti337 – 3371R → K in BAA03167. (PubMed:8161820)Curated
    Sequence conflicti341 – 3411E → K in BAA03167. (PubMed:8161820)Curated
    Sequence conflicti350 – 3523QHL → RCI in BAA03167. (PubMed:8161820)Curated
    Sequence conflicti414 – 4141T → R in BAA03167. (PubMed:8161820)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D14083 Genomic DNA. Translation: BAA03167.1.
    BA000036 Genomic DNA. Translation: BAB99997.1.
    BX927155 Genomic DNA. Translation: CAF21266.1.
    PIRiI40336.
    RefSeqiNP_601805.1. NC_003450.3.
    YP_226845.1. NC_006958.1.

    Genome annotation databases

    EnsemblBacteriaiBAB99997; BAB99997; BAB99997.
    CAF21266; CAF21266; cg2885.
    GeneIDi1020551.
    KEGGicgb:cg2885.
    cgl:NCgl2515.
    PATRICi21497254. VBICorGlu203724_2540.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D14083 Genomic DNA. Translation: BAA03167.1 .
    BA000036 Genomic DNA. Translation: BAB99997.1 .
    BX927155 Genomic DNA. Translation: CAF21266.1 .
    PIRi I40336.
    RefSeqi NP_601805.1. NC_003450.3.
    YP_226845.1. NC_006958.1.

    3D structure databases

    ProteinModelPortali P46395.
    SMRi P46395. Positions 1-421.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 196627.cg2885.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB99997 ; BAB99997 ; BAB99997 .
    CAF21266 ; CAF21266 ; cg2885 .
    GeneIDi 1020551.
    KEGGi cgb:cg2885.
    cgl:NCgl2515.
    PATRICi 21497254. VBICorGlu203724_2540.

    Phylogenomic databases

    eggNOGi COG0161.
    HOGENOMi HOG000020209.
    KOi K00833.
    OMAi FTTPDED.
    OrthoDBi EOG6QVRHN.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00160 .

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 2 hits.
    HAMAPi MF_00834. BioA.
    InterProi IPR005814. Aminotrans_3.
    IPR005815. BioA.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view ]
    PANTHERi PTHR11986. PTHR11986. 1 hit.
    Pfami PF00202. Aminotran_3. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000521. Transaminase_4ab_Lys_Orn. 1 hit.
    SUPFAMi SSF53383. SSF53383. 1 hit.
    TIGRFAMsi TIGR00508. bioA. 1 hit.
    PROSITEi PS00600. AA_TRANSFER_CLASS_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genomic organization of the biotin biosynthetic genes of coryneform bacteria: cloning and sequencing of the bioA-bioD genes from Brevibacterium flavum."
      Hatakeyama K., Hohama K., Vertes A.A., Kobayashi M., Kurusu Y., Yukawa H.
      DNA Seq. 4:177-184(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
      Strain: MJ233.
    2. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
      Ikeda M., Nakagawa S.
      Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

    Entry informationi

    Entry nameiBIOA_CORGL
    AccessioniPrimary (citable) accession number: P46395
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: August 13, 2002
    Last modified: October 1, 2014
    This is version 99 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3