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P46394 (DNAB_MYCLE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Replicative DNA helicase
EC=3.6.4.12

Cleaved into the following chain:

  1. Mle dnaB intein
Gene names
Name:dnaB
Ordered Locus Names:ML2680
ORF Names:MLCB1913.16c
OrganismMycobacterium leprae [Complete proteome] [HAMAP]
Taxonomic identifier1769 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length604 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity By similarity.

Catalytic activity

ATP + H2O = ADP + phosphate.

Post-translational modification

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential.

Sequence similarities

Belongs to the helicase family. DnaB subfamily.

Contains 1 SF4 helicase domain.

Ontologies

Keywords
   Biological processDNA replication
   Cellular componentPrimosome
   DomainRepeat
   LigandATP-binding
DNA-binding
Nucleotide-binding
   Molecular functionHelicase
Hydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processDNA replication, synthesis of RNA primer

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentalpha DNA polymerase:primase complex

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

DNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

DNA helicase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 233233Replicative DNA helicase, 1st part Potential
PRO_0000013279
Chain234 – 378145Mle dnaB intein Potential
PRO_0000013280
Chain379 – 604226Replicative DNA helicase, 2nd part Potential
PRO_0000013281

Regions

Domain196 – 462267SF4 helicase; first part
Domain341 – 604264SF4 helicase; second part
Nucleotide binding227 – 2348ATP Potential

Experimental info

Sequence conflict252 – 2576DVAVGD → ELRPRS in AAB53118. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P46394 [UniParc].

Last modified April 27, 2001. Version 2.
Checksum: D140BE34CE90C43D

FASTA60465,876
        10         20         30         40         50         60 
MAVVDDLAHS GMDAVPPSED FGRQPPQDLA AEQSVLGGML LSKDAIADVL ERLRSGDFYR 

        70         80         90        100        110        120 
PAHQNVYDAI LDLYGRGEPA DAVTVAAELD RRGLLRRIGG APYLHTLIST VPTAANAGYY 

       130        140        150        160        170        180 
AGIVAEKALL RRLVEAGTRV VQYGYAGAEG ADVAEVVDRA QAEIYDVAEC RLSENYVPLE 

       190        200        210        220        230        240 
DLLQPTMDDL DAIASNGGIS RGAPTGFTEL DEVTNGLHPG QMIIVAARPG VGKALALDTP 

       250        260        270        280        290        300 
LPTPTGWTAM GDVAVGDELL AVDEAPTRVV AATEVMLGRP CYEIEFSDGT VIVADAQHQW 

       310        320        330        340        350        360 
PTSYGIRTSA QLRCGLDIIA AAGSTPRHAG RLTTAAFMAP VLCIDSVRRV RSVPVRCVEV 

       370        380        390        400        410        420 
DNAAHLYLAG RGMVPTHNST LGLDFMRSCS IKHRLASVIF SLEMSKSEIV MRLLSAEAKI 

       430        440        450        460        470        480 
KLADMRSGRM TDDDWTRLAR RMSEISEAPL YIDDSPNLTM MEIRAKARRL RQKTNLKLVV 

       490        500        510        520        530        540 
VDYLQLMTSG KRFESRQVEV SEFSRHLKLL AKELELPVVA ISQLNRSPEQ RTDKKPMLSD 

       550        560        570        580        590        600 
LRESGSLEQD ADVVILLHRP DAFDRDDPRG GEADLILAKH RNGPTKTVTV AHQLHLSRFT 


NMAR

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AL022118 Genomic DNA. Translation: CAA17948.1.
AL022118 Genomic DNA. Translation: CAA17949.1.
AL583926 Genomic DNA. Translation: CAC32212.1.
L39923 Genomic DNA. Translation: AAB53118.1.
PIRF87244.
RefSeqNP_302709.1. NC_002677.1.

3D structure databases

ProteinModelPortalP46394.
SMRP46394. Positions 24-168.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBMYCT00000027967; EBMYCP00000027573; EBMYCG00000027962.
GeneID910801.
GenomeReviewsGene locus ML2680 in contig AL450380_GR.
KEGGmle:ML2680.
NMPDRfig|272631.1.peg.1581.
PATRIC18060517. VBIMycLep78757_5172.

Organism-specific databases

LepromaML2680.
CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000016654.
HOGENOMHBG734566.
OMAKNQRTFA.
ProtClustDBCLSK2460635.

Enzyme and pathway databases

BioCycMLEP272631:ML2680-MONOMER.

Family and domain databases

InterProIPR007692. DNA_helicase_DnaB.
IPR007694. DNA_helicase_DnaB-like_C.
IPR007693. DNA_helicase_DnaB-like_N.
IPR016136. DNA_helicase_N/primase_C.
IPR003587. Hedgehog_hint_N.
[Graphical view]
Gene3DG3DSA:1.10.860.10. DnaB_N. 1 hit.
KOK02314.
PfamPF00772. DnaB. 1 hit.
PF03796. DnaB_C. 2 hits.
[Graphical view]
SMARTSM00306. HintN. 1 hit.
[Graphical view]
SUPFAMSSF48024. DNA_helicase_DnaB-like_N. 1 hit.
TIGRFAMsTIGR00665. DnaB. 1 hit.
PROSITEPS50818. INTEIN_C_TER. False negative.
PS50817. INTEIN_N_TER. False negative.
PS51199. SF4_HELICASE. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNAB_MYCLE
AccessionPrimary (citable) accession number: P46394
Secondary accession number(s): O53124
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: April 27, 2001
Last modified: January 25, 2012
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Intein-containing proteins

List of intein-containing protein entries

SIMILARITY comments

Index of protein domains and families

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