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P46392

- BCCA_MYCLE

UniProt

P46392 - BCCA_MYCLE

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Protein

Acetyl-/propionyl-coenzyme A carboxylase alpha chain

Gene

bccA

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

This protein carries two functions: biotin carboxyl carrier protein and biotin carboxyltransferase.By similarity

Catalytic activityi

ATP + biotin-[carboxyl-carrier-protein] + CO2 = ADP + phosphate + carboxy-biotin-[carboxyl-carrier-protein].

Cofactori

biotinNote: Biotin.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei123 – 1231ATPBy similarity
Binding sitei207 – 2071ATPBy similarity
Binding sitei242 – 2421ATPBy similarity
Active sitei299 – 2991By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. biotin carboxylase activity Source: UniProtKB-EC
  3. metal ion binding Source: InterPro

GO - Biological processi

  1. fatty acid biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

ATP-binding, Biotin, Nucleotide-binding

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-5080-MONOMER.
UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetyl-/propionyl-coenzyme A carboxylase alpha chain
Including the following 2 domains:
Biotin carboxylase (EC:6.3.4.14)
Biotin carboxyl carrier protein
Short name:
BCCP
Gene namesi
Name:bccA
Ordered Locus Names:ML0726
ORF Names:B1308_C1_129
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000000806: Chromosome

Organism-specific databases

LepromaiML0726.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 598598Acetyl-/propionyl-coenzyme A carboxylase alpha chainPRO_0000146797Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei564 – 5641N6-biotinyllysineBy similarityPROSITE-ProRule annotation

Interactioni

Subunit structurei

Multimer comprised of 2 different subunits, the larger one (63/64 kDa) has biotin carboxylase and biotin carrier functions, while the smaller subunit possesses carboxyltransferase and substrate binding activity.By similarity

Protein-protein interaction databases

STRINGi272631.ML0726.

Structurei

3D structure databases

ProteinModelPortaliP46392.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 452445Biotin carboxylationAdd
BLAST
Domaini127 – 324198ATP-graspPROSITE-ProRule annotationAdd
BLAST
Domaini522 – 59877Biotinyl-bindingPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 ATP-grasp domain.PROSITE-ProRule annotation
Contains 1 biotin carboxylation domain.Curated
Contains 1 biotinyl-binding domain.CuratedPROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG4770.
HOGENOMiHOG000008988.
KOiK11263.
OMAiVCFSGHA.
OrthoDBiEOG6CVV6Z.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR001882. Biotin_BS.
IPR011764. Biotin_carboxylation_dom.
IPR005482. Biotin_COase_C.
IPR000089. Biotin_lipoyl.
IPR005481. CarbamoylP_synth_lsu_N.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR016185. PreATP-grasp_dom.
IPR011054. Rudment_hybrid_motif.
IPR011053. Single_hybrid_motif.
[Graphical view]
PfamiPF02785. Biotin_carb_C. 1 hit.
PF00364. Biotin_lipoyl. 1 hit.
PF00289. CPSase_L_chain. 1 hit.
PF02786. CPSase_L_D2. 1 hit.
[Graphical view]
SMARTiSM00878. Biotin_carb_C. 1 hit.
[Graphical view]
SUPFAMiSSF51230. SSF51230. 1 hit.
SSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS50979. BC. 1 hit.
PS00188. BIOTIN. 1 hit.
PS50968. BIOTINYL_LIPOYL. 1 hit.
PS00866. CPSASE_1. 1 hit.
PS00867. CPSASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P46392-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASHASSRIA KVLVANRGEI AVRVIRAARD ARLPSVAVYA EPDAEAPHVR
60 70 80 90 100
LADEAFALGG HTSAESYLDF GKILDAAAKS GANAIHPGYG FLAENADFAQ
110 120 130 140 150
AVIDAGLIWI GPSPQSIRDL GDKVTARHIA ARAQAPLVPG TPDPVKNADE
160 170 180 190 200
VVAFAKEHGV PIAIKAAFGG GGKGMKVART LEEISELYES AVREATVAFG
210 220 230 240 250
RGECFVERYL DKPRHVEAQV IADQHGNIVV AGTRDCSLQR RFQKLVEEAP
260 270 280 290 300
APFLTDAQRK EIHESAKRIC KEAHYYGAGT VEYLVGQDGL ISFLEVNTRL
310 320 330 340 350
QVEHPVTEET TGIDLVLQQF KIANGEKLEL IKDPIPCGHA IEFRINGEDA
360 370 380 390 400
GRNFLPSPGP VSKFHPPTGP GVRLDSGVET GSVIGGQFDS MLAKLIVHGA
410 420 430 440 450
TRQEALARAR RALDEFEVEG LATVIPFHRA VVSDPALIGD NNSFSVHTRW
460 470 480 490 500
IETEWNNTIE PFIDNQPLDE EDTRPQQTVI VEVDGRRLEV SLPADLALAN
510 520 530 540 550
PAGCNPAGVI RKKPKPRKRG GHTGAATSGD AVTAPMQGTV VKVAVAEGQT
560 570 580 590
VMTGDLVVVL EAMKMENPVT AHKDGIITGL AVEAGTAITQ GTVLAEIK
Length:598
Mass (Da):63,863
Last modified:April 27, 2001 - v2
Checksum:i5F2E291D7C54515D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti30 – 301D → H in CAA45070. (PubMed:7909542)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63470 Genomic DNA. Translation: CAA45070.1.
U00012 Genomic DNA. Translation: AAA85920.1.
AL583919 Genomic DNA. Translation: CAC30235.1.
PIRiA55579.
G86999.
RefSeqiNP_301567.1. NC_002677.1.
WP_010907891.1. NC_002677.1.

Genome annotation databases

EnsemblBacteriaiCAC30235; CAC30235; CAC30235.
GeneIDi909662.
KEGGimle:ML0726.
PATRICi18052750. VBIMycLep78757_1321.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63470 Genomic DNA. Translation: CAA45070.1 .
U00012 Genomic DNA. Translation: AAA85920.1 .
AL583919 Genomic DNA. Translation: CAC30235.1 .
PIRi A55579.
G86999.
RefSeqi NP_301567.1. NC_002677.1.
WP_010907891.1. NC_002677.1.

3D structure databases

ProteinModelPortali P46392.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272631.ML0726.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAC30235 ; CAC30235 ; CAC30235 .
GeneIDi 909662.
KEGGi mle:ML0726.
PATRICi 18052750. VBIMycLep78757_1321.

Organism-specific databases

Lepromai ML0726.

Phylogenomic databases

eggNOGi COG4770.
HOGENOMi HOG000008988.
KOi K11263.
OMAi VCFSGHA.
OrthoDBi EOG6CVV6Z.

Enzyme and pathway databases

UniPathwayi UPA00094 .
BioCyci RETL1328306-WGS:GSTH-5080-MONOMER.

Family and domain databases

Gene3Di 3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
InterProi IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR001882. Biotin_BS.
IPR011764. Biotin_carboxylation_dom.
IPR005482. Biotin_COase_C.
IPR000089. Biotin_lipoyl.
IPR005481. CarbamoylP_synth_lsu_N.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR016185. PreATP-grasp_dom.
IPR011054. Rudment_hybrid_motif.
IPR011053. Single_hybrid_motif.
[Graphical view ]
Pfami PF02785. Biotin_carb_C. 1 hit.
PF00364. Biotin_lipoyl. 1 hit.
PF00289. CPSase_L_chain. 1 hit.
PF02786. CPSase_L_D2. 1 hit.
[Graphical view ]
SMARTi SM00878. Biotin_carb_C. 1 hit.
[Graphical view ]
SUPFAMi SSF51230. SSF51230. 1 hit.
SSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
PROSITEi PS50975. ATP_GRASP. 1 hit.
PS50979. BC. 1 hit.
PS00188. BIOTIN. 1 hit.
PS50968. BIOTINYL_LIPOYL. 1 hit.
PS00866. CPSASE_1. 1 hit.
PS00867. CPSASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Lipid synthesis in mycobacteria: characterization of the biotin carboxyl carrier protein genes from Mycobacterium leprae and M. tuberculosis."
    Norman E., de Smet K.A.L., Stoker N.G., Ratledge C., Wheeler P.R., Dale J.W.
    J. Bacteriol. 176:2525-2531(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Smith D.R., Robison K.
    Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TN.

Entry informationi

Entry nameiBCCA_MYCLE
AccessioniPrimary (citable) accession number: P46392
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: April 27, 2001
Last modified: November 26, 2014
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3