P46392 (BCCA_MYCLE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetyl-/propionyl-coenzyme A carboxylase alpha chain

Including the following 2 domains:

1. Biotin carboxylase
   EC=6.3.4.14
2. Biotin carboxyl carrier protein
   Short name=BCCP

Gene names

Name:	bccA
Ordered Locus Names:	ML0726
ORF Names:	B1308_C1_129

Organism

Mycobacterium leprae [Complete proteome] [HAMAP]

Taxonomic identifier

1769 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	598 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This protein carries two functions: biotin carboxyl carrier protein and biotin carboxyltransferase By similarity.
Catalytic activity	ATP + biotin-[carboxyl-carrier-protein] + CO2 = ADP + phosphate + carboxy-biotin-[carboxyl-carrier-protein].
Cofactor	Biotin.
Pathway	Lipid metabolism; fatty acid biosynthesis.
Subunit structure	Multimer comprised of 2 different subunits, the larger one (63/64 kDa) has biotin carboxylase and biotin carrier functions, while the smaller subunit possesses carboxyltransferase and substrate binding activity By similarity.
Sequence similarities	Contains 1 ATP-grasp domain. Contains 1 biotin carboxylation domain. Contains 1 biotinyl-binding domain.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Ligand	ATP-binding Biotin Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW biotin carboxylase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 598	598	Acetyl-/propionyl-coenzyme A carboxylase alpha chain		PRO_0000146797
Regions
	Domain	8 – 452	445	Biotin carboxylation
	Domain	127 – 324	198	ATP-grasp
	Domain	530 – 597	68	Biotinyl-binding
Sites
	Active site	299	1	By similarity
	Binding site	123	1	ATP By similarity
	Binding site	207	1	ATP By similarity
	Binding site	242	1	ATP By similarity
Amino acid modifications
	Modified residue	564	1	N6-biotinyllysine By similarity
Experimental info
	Sequence conflict	30	1	D → H in CAA45070. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P46392 [UniParc]. Last modified April 27, 2001. Version 2. Checksum: 5F2E291D7C54515D	FASTA	598	63,863
	10 20 30 40 50 60 MASHASSRIA KVLVANRGEI AVRVIRAARD ARLPSVAVYA EPDAEAPHVR LADEAFALGG 70 80 90 100 110 120 HTSAESYLDF GKILDAAAKS GANAIHPGYG FLAENADFAQ AVIDAGLIWI GPSPQSIRDL 130 140 150 160 170 180 GDKVTARHIA ARAQAPLVPG TPDPVKNADE VVAFAKEHGV PIAIKAAFGG GGKGMKVART 190 200 210 220 230 240 LEEISELYES AVREATVAFG RGECFVERYL DKPRHVEAQV IADQHGNIVV AGTRDCSLQR 250 260 270 280 290 300 RFQKLVEEAP APFLTDAQRK EIHESAKRIC KEAHYYGAGT VEYLVGQDGL ISFLEVNTRL 310 320 330 340 350 360 QVEHPVTEET TGIDLVLQQF KIANGEKLEL IKDPIPCGHA IEFRINGEDA GRNFLPSPGP 370 380 390 400 410 420 VSKFHPPTGP GVRLDSGVET GSVIGGQFDS MLAKLIVHGA TRQEALARAR RALDEFEVEG 430 440 450 460 470 480 LATVIPFHRA VVSDPALIGD NNSFSVHTRW IETEWNNTIE PFIDNQPLDE EDTRPQQTVI 490 500 510 520 530 540 VEVDGRRLEV SLPADLALAN PAGCNPAGVI RKKPKPRKRG GHTGAATSGD AVTAPMQGTV 550 560 570 580 590 VKVAVAEGQT VMTGDLVVVL EAMKMENPVT AHKDGIITGL AVEAGTAITQ GTVLAEIK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Lipid synthesis in mycobacteria: characterization of the biotin carboxyl carrier protein genes from Mycobacterium leprae and M. tuberculosis." Norman E., de Smet K.A.L., Stoker N.G., Ratledge C., Wheeler P.R., Dale J.W. J. Bacteriol. 176:2525-2531(1994) [PubMed: 7909542] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Smith D.R., Robison K. Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Massive gene decay in the leprosy bacillus." Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S. , Feltwell T., Fraser A., Hamlin N., Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S., Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R., Barrell B.G. Nature 409:1007-1011(2001) [PubMed: 11234002] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TN.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X63470 Genomic DNA. Translation: CAA45070.1. U00012 Genomic DNA. Translation: AAA85920.1. AL583919 Genomic DNA. Translation: CAC30235.1.
PIR	A55579. G86999.
RefSeq	NP_301567.1. NC_002677.1.
3D structure databases
ProteinModelPortal	P46392.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000029281; EBMYCP00000028887; EBMYCG00000029276.
GeneID	909662.
GenomeReviews	Gene locus ML0726 in contig AL450380_GR.
KEGG	mle:ML0726.
NMPDR	fig|272631.1.peg.439.
PATRIC	18052750. VBIMycLep78757_1321.
Organism-specific databases
Leproma	ML0726.
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000015717.
HOGENOM	HBG535236.
OMA	DAVAAPM.
ProtClustDB	CLSK2460473.
Enzyme and pathway databases
BioCyc	MLEP272631:ML0726-MONOMER.
Family and domain databases
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR001882. Biotin_BS. IPR011764. Biotin_carboxylation_dom. IPR005482. Biotin_COase_C. IPR000089. Biotin_lipoyl. IPR005479. CarbamoylP_synth_lsu_ATP-bd. IPR005481. CarbamoylP_synth_lsu_N. IPR013817. Pre-ATP_grasp. IPR016185. PreATP-grasp-like. IPR011054. Rudment_hybrid_motif. IPR011053. Single_hybrid_motif. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
KO	K11263.
Pfam	PF02785. Biotin_carb_C. 1 hit. PF00364. Biotin_lipoyl. 1 hit. PF00289. CPSase_L_chain. 1 hit. PF02786. CPSase_L_D2. 1 hit. [Graphical view]
SMART	SM00878. Biotin_carb_C. 1 hit. [Graphical view]
SUPFAM	SSF51230. Hybrid_motif. 1 hit. SSF52440. PreATP-grasp-like. 1 hit. SSF51246. Rudmnt_hyb_motif. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS50979. BC. 1 hit. PS00188. BIOTIN. 1 hit. PS50968. BIOTINYL_LIPOYL. 1 hit. PS00866. CPSASE_1. 1 hit. PS00867. CPSASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BCCA_MYCLE

Accession

Primary (citable) accession number: P46392

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	April 27, 2001
Last modified:	January 25, 2012
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program