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P46392 (BCCA_MYCLE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Acetyl-/propionyl-coenzyme A carboxylase alpha chain

Including the following 2 domains:

  1. Biotin carboxylase
    EC=6.3.4.14
  2. Biotin carboxyl carrier protein
    Short name=BCCP
Gene names
Name:bccA
Ordered Locus Names:ML0726
ORF Names:B1308_C1_129
OrganismMycobacterium leprae (strain TN) [Complete proteome] [HAMAP]
Taxonomic identifier272631 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium

Protein attributes

Sequence length598 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This protein carries two functions: biotin carboxyl carrier protein and biotin carboxyltransferase By similarity.

Catalytic activity

ATP + biotin-[carboxyl-carrier-protein] + CO2 = ADP + phosphate + carboxy-biotin-[carboxyl-carrier-protein].

Cofactor

Biotin.

Pathway

Lipid metabolism; fatty acid biosynthesis.

Subunit structure

Multimer comprised of 2 different subunits, the larger one (63/64 kDa) has biotin carboxylase and biotin carrier functions, while the smaller subunit possesses carboxyltransferase and substrate binding activity By similarity.

Sequence similarities

Contains 1 ATP-grasp domain.

Contains 1 biotin carboxylation domain.

Contains 1 biotinyl-binding domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 598598Acetyl-/propionyl-coenzyme A carboxylase alpha chain
PRO_0000146797

Regions

Domain8 – 452445Biotin carboxylation
Domain127 – 324198ATP-grasp
Domain530 – 59768Biotinyl-binding

Sites

Active site2991 By similarity
Binding site1231ATP By similarity
Binding site2071ATP By similarity
Binding site2421ATP By similarity

Amino acid modifications

Modified residue5641N6-biotinyllysine By similarity

Experimental info

Sequence conflict301D → H in CAA45070. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P46392 [UniParc].

Last modified April 27, 2001. Version 2.
Checksum: 5F2E291D7C54515D

FASTA59863,863
        10         20         30         40         50         60 
MASHASSRIA KVLVANRGEI AVRVIRAARD ARLPSVAVYA EPDAEAPHVR LADEAFALGG 

        70         80         90        100        110        120 
HTSAESYLDF GKILDAAAKS GANAIHPGYG FLAENADFAQ AVIDAGLIWI GPSPQSIRDL 

       130        140        150        160        170        180 
GDKVTARHIA ARAQAPLVPG TPDPVKNADE VVAFAKEHGV PIAIKAAFGG GGKGMKVART 

       190        200        210        220        230        240 
LEEISELYES AVREATVAFG RGECFVERYL DKPRHVEAQV IADQHGNIVV AGTRDCSLQR 

       250        260        270        280        290        300 
RFQKLVEEAP APFLTDAQRK EIHESAKRIC KEAHYYGAGT VEYLVGQDGL ISFLEVNTRL 

       310        320        330        340        350        360 
QVEHPVTEET TGIDLVLQQF KIANGEKLEL IKDPIPCGHA IEFRINGEDA GRNFLPSPGP 

       370        380        390        400        410        420 
VSKFHPPTGP GVRLDSGVET GSVIGGQFDS MLAKLIVHGA TRQEALARAR RALDEFEVEG 

       430        440        450        460        470        480 
LATVIPFHRA VVSDPALIGD NNSFSVHTRW IETEWNNTIE PFIDNQPLDE EDTRPQQTVI 

       490        500        510        520        530        540 
VEVDGRRLEV SLPADLALAN PAGCNPAGVI RKKPKPRKRG GHTGAATSGD AVTAPMQGTV 

       550        560        570        580        590 
VKVAVAEGQT VMTGDLVVVL EAMKMENPVT AHKDGIITGL AVEAGTAITQ GTVLAEIK 

« Hide

References

« Hide 'large scale' references
[1]"Lipid synthesis in mycobacteria: characterization of the biotin carboxyl carrier protein genes from Mycobacterium leprae and M. tuberculosis."
Norman E., de Smet K.A.L., Stoker N.G., Ratledge C., Wheeler P.R., Dale J.W.
J. Bacteriol. 176:2525-2531(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]Smith D.R., Robison K.
Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Massive gene decay in the leprosy bacillus."
Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S. expand/collapse author list , Feltwell T., Fraser A., Hamlin N., Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S., Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R., Barrell B.G.
Nature 409:1007-1011(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: TN.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X63470 Genomic DNA. Translation: CAA45070.1.
U00012 Genomic DNA. Translation: AAA85920.1.
AL583919 Genomic DNA. Translation: CAC30235.1.
PIRA55579.
G86999.
RefSeqNP_301567.1. NC_002677.1.

3D structure databases

ProteinModelPortalP46392.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272631.ML0726.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAC30235; CAC30235; CAC30235.
GeneID909662.
KEGGmle:ML0726.
PATRIC18052750. VBIMycLep78757_1321.

Organism-specific databases

LepromaML0726.
CMRSearch...

Phylogenomic databases

eggNOGCOG4770.
HOGENOMHOG000008988.
KOK11263.
OMAVCFSGHA.
OrthoDBEOG6CVV6Z.

Enzyme and pathway databases

BioCycRETL1328306-WGS:GSTH-5080-MONOMER.
UniPathwayUPA00094.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR001882. Biotin_BS.
IPR011764. Biotin_carboxylation_dom.
IPR005482. Biotin_COase_C.
IPR000089. Biotin_lipoyl.
IPR005481. CarbamoylP_synth_lsu_N.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR016185. PreATP-grasp_dom.
IPR011054. Rudment_hybrid_motif.
IPR011053. Single_hybrid_motif.
[Graphical view]
PfamPF02785. Biotin_carb_C. 1 hit.
PF00364. Biotin_lipoyl. 1 hit.
PF00289. CPSase_L_chain. 1 hit.
PF02786. CPSase_L_D2. 1 hit.
[Graphical view]
SMARTSM00878. Biotin_carb_C. 1 hit.
[Graphical view]
SUPFAMSSF51230. SSF51230. 1 hit.
SSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS50979. BC. 1 hit.
PS00188. BIOTIN. 1 hit.
PS50968. BIOTINYL_LIPOYL. 1 hit.
PS00866. CPSASE_1. 1 hit.
PS00867. CPSASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBCCA_MYCLE
AccessionPrimary (citable) accession number: P46392
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: April 27, 2001
Last modified: July 9, 2014
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways