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P46392

- BCCA_MYCLE

UniProt

P46392 - BCCA_MYCLE

Protein

Acetyl-/propionyl-coenzyme A carboxylase alpha chain

Gene

bccA

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 2 (27 Apr 2001)
      Previous versions | rss
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    Functioni

    This protein carries two functions: biotin carboxyl carrier protein and biotin carboxyltransferase.By similarity

    Catalytic activityi

    ATP + biotin-[carboxyl-carrier-protein] + CO2 = ADP + phosphate + carboxy-biotin-[carboxyl-carrier-protein].

    Cofactori

    Biotin.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei123 – 1231ATPBy similarity
    Binding sitei207 – 2071ATPBy similarity
    Binding sitei242 – 2421ATPBy similarity
    Active sitei299 – 2991By similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. biotin carboxylase activity Source: UniProtKB-EC
    3. metal ion binding Source: InterPro

    GO - Biological processi

    1. fatty acid biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

    Keywords - Ligandi

    ATP-binding, Biotin, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciRETL1328306-WGS:GSTH-5080-MONOMER.
    UniPathwayiUPA00094.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acetyl-/propionyl-coenzyme A carboxylase alpha chain
    Including the following 2 domains:
    Biotin carboxylase (EC:6.3.4.14)
    Biotin carboxyl carrier protein
    Short name:
    BCCP
    Gene namesi
    Name:bccA
    Ordered Locus Names:ML0726
    ORF Names:B1308_C1_129
    OrganismiMycobacterium leprae (strain TN)
    Taxonomic identifieri272631 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
    ProteomesiUP000000806: Chromosome

    Organism-specific databases

    LepromaiML0726.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 598598Acetyl-/propionyl-coenzyme A carboxylase alpha chainPRO_0000146797Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei564 – 5641N6-biotinyllysineBy similarity

    Interactioni

    Subunit structurei

    Multimer comprised of 2 different subunits, the larger one (63/64 kDa) has biotin carboxylase and biotin carrier functions, while the smaller subunit possesses carboxyltransferase and substrate binding activity.By similarity

    Protein-protein interaction databases

    STRINGi272631.ML0726.

    Structurei

    3D structure databases

    ProteinModelPortaliP46392.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini8 – 452445Biotin carboxylationAdd
    BLAST
    Domaini127 – 324198ATP-graspPROSITE-ProRule annotationAdd
    BLAST
    Domaini530 – 59768Biotinyl-bindingAdd
    BLAST

    Sequence similaritiesi

    Contains 1 ATP-grasp domain.PROSITE-ProRule annotation
    Contains 1 biotin carboxylation domain.Curated
    Contains 1 biotinyl-binding domain.Curated

    Phylogenomic databases

    eggNOGiCOG4770.
    HOGENOMiHOG000008988.
    KOiK11263.
    OMAiVCFSGHA.
    OrthoDBiEOG6CVV6Z.

    Family and domain databases

    Gene3Di3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.20. 1 hit.
    InterProiIPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR001882. Biotin_BS.
    IPR011764. Biotin_carboxylation_dom.
    IPR005482. Biotin_COase_C.
    IPR000089. Biotin_lipoyl.
    IPR005481. CarbamoylP_synth_lsu_N.
    IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
    IPR016185. PreATP-grasp_dom.
    IPR011054. Rudment_hybrid_motif.
    IPR011053. Single_hybrid_motif.
    [Graphical view]
    PfamiPF02785. Biotin_carb_C. 1 hit.
    PF00364. Biotin_lipoyl. 1 hit.
    PF00289. CPSase_L_chain. 1 hit.
    PF02786. CPSase_L_D2. 1 hit.
    [Graphical view]
    SMARTiSM00878. Biotin_carb_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF51230. SSF51230. 1 hit.
    SSF51246. SSF51246. 1 hit.
    SSF52440. SSF52440. 1 hit.
    PROSITEiPS50975. ATP_GRASP. 1 hit.
    PS50979. BC. 1 hit.
    PS00188. BIOTIN. 1 hit.
    PS50968. BIOTINYL_LIPOYL. 1 hit.
    PS00866. CPSASE_1. 1 hit.
    PS00867. CPSASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P46392-1 [UniParc]FASTAAdd to Basket

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    MASHASSRIA KVLVANRGEI AVRVIRAARD ARLPSVAVYA EPDAEAPHVR    50
    LADEAFALGG HTSAESYLDF GKILDAAAKS GANAIHPGYG FLAENADFAQ 100
    AVIDAGLIWI GPSPQSIRDL GDKVTARHIA ARAQAPLVPG TPDPVKNADE 150
    VVAFAKEHGV PIAIKAAFGG GGKGMKVART LEEISELYES AVREATVAFG 200
    RGECFVERYL DKPRHVEAQV IADQHGNIVV AGTRDCSLQR RFQKLVEEAP 250
    APFLTDAQRK EIHESAKRIC KEAHYYGAGT VEYLVGQDGL ISFLEVNTRL 300
    QVEHPVTEET TGIDLVLQQF KIANGEKLEL IKDPIPCGHA IEFRINGEDA 350
    GRNFLPSPGP VSKFHPPTGP GVRLDSGVET GSVIGGQFDS MLAKLIVHGA 400
    TRQEALARAR RALDEFEVEG LATVIPFHRA VVSDPALIGD NNSFSVHTRW 450
    IETEWNNTIE PFIDNQPLDE EDTRPQQTVI VEVDGRRLEV SLPADLALAN 500
    PAGCNPAGVI RKKPKPRKRG GHTGAATSGD AVTAPMQGTV VKVAVAEGQT 550
    VMTGDLVVVL EAMKMENPVT AHKDGIITGL AVEAGTAITQ GTVLAEIK 598
    Length:598
    Mass (Da):63,863
    Last modified:April 27, 2001 - v2
    Checksum:i5F2E291D7C54515D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti30 – 301D → H in CAA45070. (PubMed:7909542)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X63470 Genomic DNA. Translation: CAA45070.1.
    U00012 Genomic DNA. Translation: AAA85920.1.
    AL583919 Genomic DNA. Translation: CAC30235.1.
    PIRiA55579.
    G86999.
    RefSeqiNP_301567.1. NC_002677.1.
    WP_010907891.1. NC_002677.1.

    Genome annotation databases

    EnsemblBacteriaiCAC30235; CAC30235; CAC30235.
    GeneIDi909662.
    KEGGimle:ML0726.
    PATRICi18052750. VBIMycLep78757_1321.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X63470 Genomic DNA. Translation: CAA45070.1 .
    U00012 Genomic DNA. Translation: AAA85920.1 .
    AL583919 Genomic DNA. Translation: CAC30235.1 .
    PIRi A55579.
    G86999.
    RefSeqi NP_301567.1. NC_002677.1.
    WP_010907891.1. NC_002677.1.

    3D structure databases

    ProteinModelPortali P46392.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272631.ML0726.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAC30235 ; CAC30235 ; CAC30235 .
    GeneIDi 909662.
    KEGGi mle:ML0726.
    PATRICi 18052750. VBIMycLep78757_1321.

    Organism-specific databases

    Lepromai ML0726.

    Phylogenomic databases

    eggNOGi COG4770.
    HOGENOMi HOG000008988.
    KOi K11263.
    OMAi VCFSGHA.
    OrthoDBi EOG6CVV6Z.

    Enzyme and pathway databases

    UniPathwayi UPA00094 .
    BioCyci RETL1328306-WGS:GSTH-5080-MONOMER.

    Family and domain databases

    Gene3Di 3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.20. 1 hit.
    InterProi IPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR001882. Biotin_BS.
    IPR011764. Biotin_carboxylation_dom.
    IPR005482. Biotin_COase_C.
    IPR000089. Biotin_lipoyl.
    IPR005481. CarbamoylP_synth_lsu_N.
    IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
    IPR016185. PreATP-grasp_dom.
    IPR011054. Rudment_hybrid_motif.
    IPR011053. Single_hybrid_motif.
    [Graphical view ]
    Pfami PF02785. Biotin_carb_C. 1 hit.
    PF00364. Biotin_lipoyl. 1 hit.
    PF00289. CPSase_L_chain. 1 hit.
    PF02786. CPSase_L_D2. 1 hit.
    [Graphical view ]
    SMARTi SM00878. Biotin_carb_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51230. SSF51230. 1 hit.
    SSF51246. SSF51246. 1 hit.
    SSF52440. SSF52440. 1 hit.
    PROSITEi PS50975. ATP_GRASP. 1 hit.
    PS50979. BC. 1 hit.
    PS00188. BIOTIN. 1 hit.
    PS50968. BIOTINYL_LIPOYL. 1 hit.
    PS00866. CPSASE_1. 1 hit.
    PS00867. CPSASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Lipid synthesis in mycobacteria: characterization of the biotin carboxyl carrier protein genes from Mycobacterium leprae and M. tuberculosis."
      Norman E., de Smet K.A.L., Stoker N.G., Ratledge C., Wheeler P.R., Dale J.W.
      J. Bacteriol. 176:2525-2531(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Smith D.R., Robison K.
      Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: TN.

    Entry informationi

    Entry nameiBCCA_MYCLE
    AccessioniPrimary (citable) accession number: P46392
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: April 27, 2001
    Last modified: October 1, 2014
    This is version 112 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3