P46380 (AROQ_AMYME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-dehydroquinate dehydratase
Short name=3-dehydroquinase
EC=4.2.1.10

Alternative name(s):
Type II DHQase

Gene names

Name:

aroQ

Organism

Amycolatopsis methanolica

Taxonomic identifier

1814 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Pseudonocardineae › Pseudonocardiaceae › Amycolatopsis

Protein attributes

Sequence length	20 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes a trans-dehydration via an enolate intermediate. Is involved in both the catabolism of quinate and the biosynthesis of aromatic amino acids. HAMAP MF_00169
Catalytic activity	3-dehydroquinate = 3-dehydroshikimate + H2O. HAMAP MF_00169
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7. HAMAP MF_00169
Subunit structure	Homododecamer.
Sequence similarities	Belongs to the type-II 3-dehydroquinase family.
Biophysicochemical properties	pH dependence: Optimum pH is 9.0. HAMAP MF_00169 Temperature dependence: Optimum temperature is 76 degrees Celsius. Thermostable.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Quinate metabolism
Molecular function	Lyase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW quinate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-dehydroquinate dehydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›20	›20	3-dehydroquinate dehydratase HAMAP MF_00169		PRO_0000159865
Sites
	Site	17	1	Transition state stabilizer By similarity
Experimental info
	Non-terminal residue	20	1

Sequences

	Sequence		Length	Mass (Da)
	P46380 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: C24AA183E5CFDF0A	FASTA	20	2,197
	10 20 MKVFVLNGPN LGRLGKREPA

References

[1]

"Purification and characterization of a dual function 3-dehydroquinate dehydratase from Amycolatopsis methanolica." Euverink G.J.W., Hessels G.I., Vrijbloed J.W., Coggins J.R., Dijkhuizen L. J. Gen. Microbiol. 138:2449-2457(1992) [PubMed: 1479361] [Abstract] Cited for: PROTEIN SEQUENCE. Strain: DSM 44096 / JCM 8087 / NBRC 15065 / NCIMB 11946.

Cross-references

Sequence databases
PIR	A47687.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-15327.
Family and domain databases
HAMAP	MF_00169. AroQ. [Tree]
InterPro	IPR001874. DHquinase_II. [Graphical view]
Gene3D	G3DSA:3.40.50.9100. DHquinase_II. 1 hit.
Pfam	PF01220. DHquinase_II. 1 hit. [Graphical view]
PROSITE	PS01029. DEHYDROQUINASE_II. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROQ_AMYME

Accession

Primary (citable) accession number: P46380

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 31, 2011
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program