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Protein

EPTC-inducible aldehyde dehydrogenase

Gene

thcA

Organism
Rhodococcus erythropolis (Arthrobacter picolinophilus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Degrades all aldehydes potentially generated by N dealkylation of thiocarbamates and may also participate in ethanolamine metabolism and further assimilation of degradation products by thiocarbamate-induced cytochrome P-450.

Catalytic activityi

An aldehyde + NAD+ + H2O = a carboxylate + NADH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei263 – 2631By similarity
Active sitei302 – 3021By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi219 – 2257NADBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
EPTC-inducible aldehyde dehydrogenase (EC:1.2.1.3)
Gene namesi
Name:thcA
OrganismiRhodococcus erythropolis (Arthrobacter picolinophilus)
Taxonomic identifieri1833 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesNocardiaceaeRhodococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 506505EPTC-inducible aldehyde dehydrogenasePRO_0000056453Add
BLAST

Proteomic databases

PRIDEiP46369.

Expressioni

Inductioni

By EPTC (S-ethyl dipropylcarbamothioate).

Interactioni

Protein-protein interaction databases

STRINGi234621.RER_18100.

Structurei

3D structure databases

ProteinModelPortaliP46369.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldehyde dehydrogenase family.Curated

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P46369-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKYARPGTA DAIMSFQSRY DNWIGNEWVA PVKGQYFENP TPVTGQNFCD
60 70 80 90 100
VARSTAEDIE LALDAAHAAA PAWGKTSVAE RAIILNKIAD RMEENLESIA
110 120 130 140 150
LAESWDNGKP IRETLNADIP LAIDHFRYFA GAIRAQEGSL SEINSDTVAY
160 170 180 190 200
HFHEPLGVVG QIIPWNFPIL MAVWKLAPAL AAGNAIVLKP AEQTPVSILH
210 220 230 240 250
LIGIIGDLLP AGVLNIVNGF GVEAGKPLAS SPRIKKIAFT GETTTGRLIM
260 270 280 290 300
QYASQNLIPV TLELGGKSPN VFFSDVLASN DDYQDKALEG FTMFALNQGE
310 320 330 340 350
VCTAPSRALI QEDIFDEFLA MAAIRTKAVR QGDPLDTDTM IGAQASNDQL
360 370 380 390 400
EKILSYIEIG KAEGAKVITG GERAELGGDL SGGYYVQPTV FTGNNKMRIF
410 420 430 440 450
QEIFGPVVSV TSFKDYDEAI EIANDTLYGL GAGVWSRDGG VAYRAGRDIQ
460 470 480 490 500
AGRVWTNTYH QYPAHAAFGG YKQSGIGREN HLMMLSHYQQ TKNLLVSYAQ

KAQGFF
Length:506
Mass (Da):54,994
Last modified:January 23, 2007 - v2
Checksum:iDDA25B7138B34B37
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17129 Genomic DNA. Translation: AAC77472.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17129 Genomic DNA. Translation: AAC77472.1.

3D structure databases

ProteinModelPortaliP46369.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi234621.RER_18100.

Proteomic databases

PRIDEiP46369.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Degradation of the thiocarbamate herbicide EPTC (S-ethyl dipropylcarbamothioate) and biosafening by Rhodococcus sp. strain NI86/21 involve an inducible cytochrome P-450 system and aldehyde dehydrogenase."
    Nagy I., Schoofs G., Compernolle F., Proost P., Vanderleyden J., de Mot R.
    J. Bacteriol. 177:676-687(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
    Strain: NI86/21.

Entry informationi

Entry nameiTHCA_RHOER
AccessioniPrimary (citable) accession number: P46369
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: June 24, 2015
This is version 69 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.