P46368 (DHA2_CUPNH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetaldehyde dehydrogenase 2
EC=1.2.1.3

Alternative name(s):
Acetaldehyde dehydrogenase II
Short name=ACDH-II

Gene names

Name:	acoD
Ordered Locus Names:	H16_B1960

Organism

Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha) [Complete proteome] [HAMAP]

Taxonomic identifier

381666 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus › Cupriavidus necator

Protein attributes

Sequence length	506 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the catabolism of acetoin and ethanol.
Catalytic activity	An aldehyde + NAD+ + H2O = a carboxylate + NADH.
Pathway	Alcohol metabolism; ethanol degradation; acetate from ethanol: step 2/2. Ketone degradation; acetoin degradation.
Sequence similarities	Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
Biological process	Acetoin catabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological_process	acetoin catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway ethanol catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	aldehyde dehydrogenase (NAD) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 506	506	Acetaldehyde dehydrogenase 2		PRO_0000056566
Regions
	Nucleotide binding	240 – 245	6	NAD By similarity
Sites
	Active site	262	1	By similarity
	Active site	301	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P46368 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: A8715BD93B126D4D	FASTA	506	54,882
	10 20 30 40 50 60 MNMAEIAQLG VSNPYKQQYE NYIGGAWVPP AGGEYFESTT PITGKPFTRV PRSGQQDVDA 70 80 90 100 110 120 ALDAAHAAKA AWARTSTTER ANILNRIADR IEANLKLLAV AESIDNGKPV RETTAADLPL 130 140 150 160 170 180 AVDHFRYFAG CIRAQEGGIS EIDADTIAYH FHEPLGVVGQ IIPWNFPLLM ATWKLAPALA 190 200 210 220 230 240 AGNCVVLKPA EQTPASILVL MEVIGDLLPP GVVNVINGFG LEAGKPLASS PRISKVAFTG 250 260 270 280 290 300 ETTTGRLIMQ YASQNLIPVT LELGGKSPNI FFEDVLAADD AFFDKALEGF AMFALNQGEV 310 320 330 340 350 360 CTCPSRALIQ ESIYDRFMER ALKRVAAIRQ GHPLDTGTMI GAQASAEQLE KILSYIDLGR 370 380 390 400 410 420 KEGAQCLTGG ERNVLDGDLA GGYYVKPTVF AGHNKMRIFQ EEIFGPVVSV TTFKDEEEAL 430 440 450 460 470 480 AIANDTLYGL GAGVWTRDGA RAFRMGRGIQ AGRVWTNCYH AYPAHAAFGG YKQSGIGREN 490 500 HRMMLDHYQQ TKNLLVSYSP NALGFF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and molecular characterization of the gene coding for acetaldehyde dehydrogenase II (acoD) of Alcaligenes eutrophus." Priefert H., Krueger N., Jendrossek D., Schmidt B., Steinbuechel A. J. Bacteriol. 174:899-907(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-20.
[2]	"Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia eutropha H16." Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R., Eitinger T., Ewering C., Poetter M., Schwartz E., Strittmatter A., Voss I., Gottschalk G., Steinbuechel A., Friedrich B., Bowien B. Nat. Biotechnol. 24:1257-1262(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17699 / H16 / DSM 428 / Stanier 337.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M74003 Genomic DNA. Translation: AAA21943.1. AM260480 Genomic DNA. Translation: CAJ96742.1.
PIR	A42597.
RefSeq	YP_841472.1. NC_008314.1.
3D structure databases
ProteinModelPortal	P46368.
ModBase	Search...
Protein-protein interaction databases
STRING	381666.H16_B1960.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAJ96742; CAJ96742; H16_B1960.
GeneID	4456449.
KEGG	reh:H16_B1960.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1012.
HOGENOM	HOG000271505.
KO	K00128.
OMA	IERDRAY.
ProtClustDB	CLSK716202.
Enzyme and pathway databases
BioCyc	CNEC381666:GJUJ-5660-MONOMER.
UniPathway	UPA00040. UPA00780; UER00768.
Family and domain databases
Gene3D	3.40.309.10. 1 hit. 3.40.605.10. 1 hit.
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. [Graphical view]
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHA2_CUPNH

Accession

Primary (citable) accession number: P46368
Secondary accession number(s): Q0JZT2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program