Reviewed, UniProtKB/Swiss-Prot P46368 (DHA2_RALEH)

Names and origin

Protein names

Recommended name:
    Acetaldehyde dehydrogenase 2
    EC=1.2.1.3
Alternative name(s):
    Acetaldehyde dehydrogenase II
      Short name=ACDH-II

Gene names

Name:	acoD
Ordered Locus Names:	H16_B1960

Organism

Ralstonia eutropha (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)) [Complete proteome] [HAMAP]

Taxonomic identifier

381666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	506 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the catabolism of acetoin and ethanol.
Catalytic activity	An aldehyde + NAD+ + H2O = an acid + NADH.
Pathway	Alcohol metabolism; ethanol degradation; acetate from ethanol: step 2/2. Ketone degradation; acetoin degradation.
Sequence similarities	Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
Biological process	Acetoin catabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	acetoin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aldehyde dehydrogenase (NAD) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 506	506	Acetaldehyde dehydrogenase 2		PRO_0000056566
Regions
	Nucleotide binding	240 – 245	6	NAD By similarity
Sites
	Active site	262	1	By similarity
	Active site	301	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P46368-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: A8715BD93B126D4D	FASTA	506	54,882
	10 20 30 40 50 60 MNMAEIAQLG VSNPYKQQYE NYIGGAWVPP AGGEYFESTT PITGKPFTRV PRSGQQDVDA 70 80 90 100 110 120 ALDAAHAAKA AWARTSTTER ANILNRIADR IEANLKLLAV AESIDNGKPV RETTAADLPL 130 140 150 160 170 180 AVDHFRYFAG CIRAQEGGIS EIDADTIAYH FHEPLGVVGQ IIPWNFPLLM ATWKLAPALA 190 200 210 220 230 240 AGNCVVLKPA EQTPASILVL MEVIGDLLPP GVVNVINGFG LEAGKPLASS PRISKVAFTG 250 260 270 280 290 300 ETTTGRLIMQ YASQNLIPVT LELGGKSPNI FFEDVLAADD AFFDKALEGF AMFALNQGEV 310 320 330 340 350 360 CTCPSRALIQ ESIYDRFMER ALKRVAAIRQ GHPLDTGTMI GAQASAEQLE KILSYIDLGR 370 380 390 400 410 420 KEGAQCLTGG ERNVLDGDLA GGYYVKPTVF AGHNKMRIFQ EEIFGPVVSV TTFKDEEEAL 430 440 450 460 470 480 AIANDTLYGL GAGVWTRDGA RAFRMGRGIQ AGRVWTNCYH AYPAHAAFGG YKQSGIGREN 490 500 HRMMLDHYQQ TKNLLVSYSP NALGFF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and molecular characterization of the gene coding for acetaldehyde dehydrogenase II (acoD) of Alcaligenes eutrophus." Priefert H., Krueger N., Jendrossek D., Schmidt B., Steinbuechel A. J. Bacteriol. 174:899-907(1992) [PubMed: 1732222] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-20.
[2]	"Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia eutropha H16." Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R., Eitinger T., Ewering C., Poetter M., Schwartz E., Strittmatter A., Voss I., Gottschalk G., Steinbuechel A., Friedrich B., Bowien B. Nat. Biotechnol. 24:1257-1262(2006) [PubMed: 16964242] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	M74003 Genomic DNA. Translation: AAA21943.1. AM260480 Genomic DNA. Translation: CAJ96742.1.
PIR	A42597.
RefSeq	YP_841472.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P46368.
Genome annotation databases
GeneID	4456449.
GenomeReviews	Gene locus H16_B1960 in contig AM260480_GR.
KEGG	reh:H16_B1960.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P46368.
OMA	INGQPFC
Family and domain databases
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHA2_RALEH

Accession

Primary (citable) accession number: P46368
Secondary accession number(s): Q0JZT2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 24, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)