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Protein

Potassium-activated aldehyde dehydrogenase, mitochondrial

Gene

ALD4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Potassium-activated aldehyde dehydrogenase involved in acetate formation during anaerobic growth on glucose.1 Publication

Catalytic activityi

An aldehyde + NAD(P)+ + H2O = a carboxylate + NAD(P)H.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei192Transition state stabilizerBy similarity1
Active sitei290Proton acceptorPROSITE-ProRule annotation1
Active sitei324NucleophilePROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi268 – 273NADBy similarity6

GO - Molecular functioni

  • aldehyde dehydrogenase (NAD) activity Source: SGD
  • aldehyde dehydrogenase [NAD(P)+] activity Source: SGD

GO - Biological processi

  • acetate biosynthetic process Source: SGD
  • ethanol metabolic process Source: SGD
  • NADPH regeneration Source: SGD
  • pyruvate metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciYEAST:MONOMER-13666.
ReactomeiR-SCE-196757. Metabolism of folate and pterines.
R-SCE-380612. Metabolism of serotonin.
R-SCE-5365859. RA biosynthesis pathway.
R-SCE-6798163. Choline catabolism.
R-SCE-70350. Fructose catabolism.
R-SCE-71064. Lysine catabolism.
R-SCE-71384. Ethanol oxidation.

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium-activated aldehyde dehydrogenase, mitochondrial (EC:1.2.1.5)
Alternative name(s):
K(+)-activated acetaldehyde dehydrogenase
Short name:
K(+)-ACDH
Gene namesi
Name:ALD4
Synonyms:ALD7, ALDH2
Ordered Locus Names:YOR374W
ORF Names:O6730
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR374W.
SGDiS000005901. ALD4.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial nucleoid Source: SGD
  • mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 24Mitochondrion2 PublicationsAdd BLAST24
ChainiPRO_000000716525 – 519Potassium-activated aldehyde dehydrogenase, mitochondrialAdd BLAST495

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei96PhosphoserineCombined sources1
Modified residuei216PhosphothreonineCombined sources1
Modified residuei500PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP46367.
PRIDEiP46367.
TopDownProteomicsiP46367.

PTM databases

iPTMnetiP46367.

Interactioni

Protein-protein interaction databases

BioGridi34757. 28 interactors.
DIPiDIP-4053N.
IntActiP46367. 6 interactors.
MINTiMINT-539582.

Structurei

3D structure databases

ProteinModelPortaliP46367.
SMRiP46367.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldehyde dehydrogenase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00760000118999.
HOGENOMiHOG000271505.
InParanoidiP46367.
KOiK00128.
OMAiWINLSQA.
OrthoDBiEOG092C1LLH.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P46367-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFSRSTLCLK TSASSIGRLQ LRYFSHLPMT VPIKLPNGLE YEQPTGLFIN
60 70 80 90 100
NKFVPSKQNK TFEVINPSTE EEICHIYEGR EDDVEEAVQA ADRAFSNGSW
110 120 130 140 150
NGIDPIDRGK ALYRLAELIE QDKDVIASIE TLDNGKAISS SRGDVDLVIN
160 170 180 190 200
YLKSSAGFAD KIDGRMIDTG RTHFSYTKRQ PLGVCGQIIP WNFPLLMWAW
210 220 230 240 250
KIAPALVTGN TVVLKTAEST PLSALYVSKY IPQAGIPPGV INIVSGFGKI
260 270 280 290 300
VGEAITNHPK IKKVAFTGST ATGRHIYQSA AAGLKKVTLE LGGKSPNIVF
310 320 330 340 350
ADAELKKAVQ NIILGIYYNS GEVCCAGSRV YVEESIYDKF IEEFKAASES
360 370 380 390 400
IKVGDPFDES TFQGAQTSQM QLNKILKYVD IGKNEGATLI TGGERLGSKG
410 420 430 440 450
YFIKPTVFGD VKEDMRIVKE EIFGPVVTVT KFKSADEVIN MANDSEYGLA
460 470 480 490 500
AGIHTSNINT ALKVADRVNA GTVWINTYND FHHAVPFGGF NASGLGREMS
510
VDALQNYLQV KAVRAKLDE
Length:519
Mass (Da):56,724
Last modified:November 1, 1997 - v2
Checksum:iE7D9944EA25F948E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti51N → NN AA sequence (PubMed:1989592).Curated1
Sequence conflicti63E → V AA sequence (PubMed:1989592).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z75282 Genomic DNA. Translation: CAA99705.1.
BK006948 Genomic DNA. Translation: DAA11133.1.
PIRiS67286.
RefSeqiNP_015019.1. NM_001183794.1.

Genome annotation databases

EnsemblFungiiYOR374W; YOR374W; YOR374W.
GeneIDi854556.
KEGGisce:YOR374W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z75282 Genomic DNA. Translation: CAA99705.1.
BK006948 Genomic DNA. Translation: DAA11133.1.
PIRiS67286.
RefSeqiNP_015019.1. NM_001183794.1.

3D structure databases

ProteinModelPortaliP46367.
SMRiP46367.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34757. 28 interactors.
DIPiDIP-4053N.
IntActiP46367. 6 interactors.
MINTiMINT-539582.

PTM databases

iPTMnetiP46367.

Proteomic databases

MaxQBiP46367.
PRIDEiP46367.
TopDownProteomicsiP46367.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR374W; YOR374W; YOR374W.
GeneIDi854556.
KEGGisce:YOR374W.

Organism-specific databases

EuPathDBiFungiDB:YOR374W.
SGDiS000005901. ALD4.

Phylogenomic databases

GeneTreeiENSGT00760000118999.
HOGENOMiHOG000271505.
InParanoidiP46367.
KOiK00128.
OMAiWINLSQA.
OrthoDBiEOG092C1LLH.

Enzyme and pathway databases

BioCyciYEAST:MONOMER-13666.
ReactomeiR-SCE-196757. Metabolism of folate and pterines.
R-SCE-380612. Metabolism of serotonin.
R-SCE-5365859. RA biosynthesis pathway.
R-SCE-6798163. Choline catabolism.
R-SCE-70350. Fructose catabolism.
R-SCE-71064. Lysine catabolism.
R-SCE-71384. Ethanol oxidation.

Miscellaneous databases

PROiP46367.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALDH4_YEAST
AccessioniPrimary (citable) accession number: P46367
Secondary accession number(s): D6W367, Q08898
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1997
Last modified: November 30, 2016
This is version 150 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 22200 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.