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P46367

- ALDH4_YEAST

UniProt

P46367 - ALDH4_YEAST

Protein

Potassium-activated aldehyde dehydrogenase, mitochondrial

Gene

ALD4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Potassium-activated aldehyde dehydrogenase involved in acetate formation during anaerobic growth on glucose.1 Publication

    Catalytic activityi

    An aldehyde + NAD(P)+ + H2O = a carboxylate + NAD(P)H.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei192 – 1921Transition state stabilizerBy similarity
    Active sitei290 – 2901Proton acceptorPROSITE-ProRule annotation
    Active sitei324 – 3241NucleophilePROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi268 – 2736NADBy similarity

    GO - Molecular functioni

    1. aldehyde dehydrogenase (NAD) activity Source: SGD
    2. aldehyde dehydrogenase [NAD(P)+] activity Source: SGD

    GO - Biological processi

    1. acetate biosynthetic process Source: SGD
    2. ethanol metabolic process Source: SGD
    3. NADPH regeneration Source: SGD
    4. pyruvate metabolic process Source: SGD

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciYEAST:YOR374W-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Potassium-activated aldehyde dehydrogenase, mitochondrial (EC:1.2.1.5)
    Alternative name(s):
    K(+)-activated acetaldehyde dehydrogenase
    Short name:
    K(+)-ACDH
    Gene namesi
    Name:ALD4
    Synonyms:ALD7, ALDH2
    Ordered Locus Names:YOR374W
    ORF Names:O6730
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    CYGDiYOR374w.
    SGDiS000005901. ALD4.

    Subcellular locationi

    Mitochondrion matrix 1 Publication

    GO - Cellular componenti

    1. mitochondrial nucleoid Source: SGD
    2. mitochondrion Source: SGD

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2424Mitochondrion2 PublicationsAdd
    BLAST
    Chaini25 – 519495Potassium-activated aldehyde dehydrogenase, mitochondrialPRO_0000007165Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei96 – 961Phosphoserine1 Publication
    Modified residuei216 – 2161Phosphothreonine1 Publication
    Modified residuei500 – 5001Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP46367.
    PaxDbiP46367.
    PeptideAtlasiP46367.
    PRIDEiP46367.

    Expressioni

    Gene expression databases

    GenevestigatoriP46367.

    Interactioni

    Protein-protein interaction databases

    BioGridi34757. 24 interactions.
    DIPiDIP-4053N.
    IntActiP46367. 5 interactions.
    MINTiMINT-539582.
    STRINGi4932.YOR374W.

    Structurei

    3D structure databases

    ProteinModelPortaliP46367.
    SMRiP46367. Positions 47-507.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the aldehyde dehydrogenase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG1012.
    GeneTreeiENSGT00740000115528.
    HOGENOMiHOG000271505.
    KOiK00128.
    OMAiMACWKMA.
    OrthoDBiEOG7S226Z.

    Family and domain databases

    Gene3Di3.40.309.10. 1 hit.
    3.40.605.10. 1 hit.
    InterProiIPR016161. Ald_DH/histidinol_DH.
    IPR016163. Ald_DH_C.
    IPR016160. Ald_DH_CS_CYS.
    IPR029510. Ald_DH_CS_GLU.
    IPR016162. Ald_DH_N.
    IPR015590. Aldehyde_DH_dom.
    [Graphical view]
    PfamiPF00171. Aldedh. 1 hit.
    [Graphical view]
    SUPFAMiSSF53720. SSF53720. 1 hit.
    PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
    PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P46367-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFSRSTLCLK TSASSIGRLQ LRYFSHLPMT VPIKLPNGLE YEQPTGLFIN    50
    NKFVPSKQNK TFEVINPSTE EEICHIYEGR EDDVEEAVQA ADRAFSNGSW 100
    NGIDPIDRGK ALYRLAELIE QDKDVIASIE TLDNGKAISS SRGDVDLVIN 150
    YLKSSAGFAD KIDGRMIDTG RTHFSYTKRQ PLGVCGQIIP WNFPLLMWAW 200
    KIAPALVTGN TVVLKTAEST PLSALYVSKY IPQAGIPPGV INIVSGFGKI 250
    VGEAITNHPK IKKVAFTGST ATGRHIYQSA AAGLKKVTLE LGGKSPNIVF 300
    ADAELKKAVQ NIILGIYYNS GEVCCAGSRV YVEESIYDKF IEEFKAASES 350
    IKVGDPFDES TFQGAQTSQM QLNKILKYVD IGKNEGATLI TGGERLGSKG 400
    YFIKPTVFGD VKEDMRIVKE EIFGPVVTVT KFKSADEVIN MANDSEYGLA 450
    AGIHTSNINT ALKVADRVNA GTVWINTYND FHHAVPFGGF NASGLGREMS 500
    VDALQNYLQV KAVRAKLDE 519
    Length:519
    Mass (Da):56,724
    Last modified:November 1, 1997 - v2
    Checksum:iE7D9944EA25F948E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti51 – 511N → NN AA sequence (PubMed:1989592)Curated
    Sequence conflicti63 – 631E → V AA sequence (PubMed:1989592)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z75282 Genomic DNA. Translation: CAA99705.1.
    BK006948 Genomic DNA. Translation: DAA11133.1.
    PIRiS67286.
    RefSeqiNP_015019.1. NM_001183794.1.

    Genome annotation databases

    EnsemblFungiiYOR374W; YOR374W; YOR374W.
    GeneIDi854556.
    KEGGisce:YOR374W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z75282 Genomic DNA. Translation: CAA99705.1 .
    BK006948 Genomic DNA. Translation: DAA11133.1 .
    PIRi S67286.
    RefSeqi NP_015019.1. NM_001183794.1.

    3D structure databases

    ProteinModelPortali P46367.
    SMRi P46367. Positions 47-507.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34757. 24 interactions.
    DIPi DIP-4053N.
    IntActi P46367. 5 interactions.
    MINTi MINT-539582.
    STRINGi 4932.YOR374W.

    Proteomic databases

    MaxQBi P46367.
    PaxDbi P46367.
    PeptideAtlasi P46367.
    PRIDEi P46367.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOR374W ; YOR374W ; YOR374W .
    GeneIDi 854556.
    KEGGi sce:YOR374W.

    Organism-specific databases

    CYGDi YOR374w.
    SGDi S000005901. ALD4.

    Phylogenomic databases

    eggNOGi COG1012.
    GeneTreei ENSGT00740000115528.
    HOGENOMi HOG000271505.
    KOi K00128.
    OMAi MACWKMA.
    OrthoDBi EOG7S226Z.

    Enzyme and pathway databases

    BioCyci YEAST:YOR374W-MONOMER.

    Miscellaneous databases

    NextBioi 976985.

    Gene expression databases

    Genevestigatori P46367.

    Family and domain databases

    Gene3Di 3.40.309.10. 1 hit.
    3.40.605.10. 1 hit.
    InterProi IPR016161. Ald_DH/histidinol_DH.
    IPR016163. Ald_DH_C.
    IPR016160. Ald_DH_CS_CYS.
    IPR029510. Ald_DH_CS_GLU.
    IPR016162. Ald_DH_N.
    IPR015590. Aldehyde_DH_dom.
    [Graphical view ]
    Pfami PF00171. Aldedh. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53720. SSF53720. 1 hit.
    PROSITEi PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
    PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Comparison of benzyl alcohol dehydrogenases and benzaldehyde dehydrogenases from the benzyl alcohol and mandelate pathways in Acinetobacter calcoaceticus and from the TOL-plasmid-encoded toluene pathway in Pseudomonas putida. N-terminal amino acid sequences, amino acid compositions and immunological cross-reactions."
      Chalmers R.M., Keen J.N., Fewson C.A.
      Biochem. J. 273:99-107(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 25-65.
    4. "Identification of two-dimensional gel electrophoresis resolved yeast proteins by matrix-assisted laser desorption ionization mass spectrometry."
      Larsson T., Norbeck J., Karlsson H., Karlsson K.-A., Blomberg A.
      Electrophoresis 18:418-423(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 25-34 AND 378-386.
    5. "Identification and disruption of the gene encoding the K(+)-activated acetaldehyde dehydrogenase of Saccharomyces cerevisiae."
      Tessier W.D., Meaden P.G., Dickinson F.M., Midgley M.
      FEMS Microbiol. Lett. 164:29-34(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION.
    6. "Molecular cloning, characterization, and potential roles of cytosolic and mitochondrial aldehyde dehydrogenases in ethanol metabolism in Saccharomyces cerevisiae."
      Wang X., Mann C.J., Bai Y., Ni L., Weiner H.
      J. Bacteriol. 180:822-830(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: CATALYTIC ACTIVITY.
    7. "Yeast mitochondrial dehydrogenases are associated in a supramolecular complex."
      Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N., Manon S., Schmitter J.-M.
      Biochemistry 40:9758-9769(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "Functional analysis of the ALD gene family of Saccharomyces cerevisiae during anaerobic growth on glucose: the NADP+-dependent Ald6p and Ald5p isoforms play a major role in acetate formation."
      Saint-Prix F., Boenquist L., Dequin S.
      Microbiology 150:2209-2220(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    10. "Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase."
      Reinders J., Wagner K., Zahedi R.P., Stojanovski D., Eyrich B., van der Laan M., Rehling P., Sickmann A., Pfanner N., Meisinger C.
      Mol. Cell. Proteomics 6:1896-1906(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-216 AND SER-500, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ATCC 76625 / YPH499.
    11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-96 AND SER-500, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiALDH4_YEAST
    AccessioniPrimary (citable) accession number: P46367
    Secondary accession number(s): D6W367, Q08898
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 130 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 22200 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3