SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P46367

- ALDH4_YEAST

UniProt

P46367 - ALDH4_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Potassium-activated aldehyde dehydrogenase, mitochondrial
Gene
ALD4, ALD7, ALDH2, YOR374W, O6730
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Potassium-activated aldehyde dehydrogenase involved in acetate formation during anaerobic growth on glucose.1 Publication

Catalytic activityi

An aldehyde + NAD(P)+ + H2O = a carboxylate + NAD(P)H.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei192 – 1921Transition state stabilizer By similarity
Active sitei290 – 2901Proton acceptor By similarity
Active sitei324 – 3241Nucleophile By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi268 – 2736NAD By similarity

GO - Molecular functioni

  1. aldehyde dehydrogenase (NAD) activity Source: SGD
  2. aldehyde dehydrogenase [NAD(P)+] activity Source: SGD
Complete GO annotation...

GO - Biological processi

  1. NADPH regeneration Source: SGD
  2. acetate biosynthetic process Source: SGD
  3. ethanol metabolic process Source: SGD
  4. pyruvate metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciYEAST:YOR374W-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium-activated aldehyde dehydrogenase, mitochondrial (EC:1.2.1.5)
Alternative name(s):
K(+)-activated acetaldehyde dehydrogenase
Short name:
K(+)-ACDH
Gene namesi
Name:ALD4
Synonyms:ALD7, ALDH2
Ordered Locus Names:YOR374W
ORF Names:O6730
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XV

Organism-specific databases

CYGDiYOR374w.
SGDiS000005901. ALD4.

Subcellular locationi

Mitochondrion matrix 1 Publication

GO - Cellular componenti

  1. mitochondrial nucleoid Source: SGD
  2. mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2424Mitochondrion2 Publications
Add
BLAST
Chaini25 – 519495Potassium-activated aldehyde dehydrogenase, mitochondrial
PRO_0000007165Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei96 – 961Phosphoserine1 Publication
Modified residuei216 – 2161Phosphothreonine1 Publication
Modified residuei500 – 5001Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP46367.
PaxDbiP46367.
PeptideAtlasiP46367.
PRIDEiP46367.

Expressioni

Gene expression databases

GenevestigatoriP46367.

Interactioni

Protein-protein interaction databases

BioGridi34757. 24 interactions.
DIPiDIP-4053N.
IntActiP46367. 5 interactions.
MINTiMINT-539582.
STRINGi4932.YOR374W.

Structurei

3D structure databases

ProteinModelPortaliP46367.
SMRiP46367. Positions 47-507.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG1012.
GeneTreeiENSGT00740000115528.
HOGENOMiHOG000271505.
KOiK00128.
OMAiMACWKMA.
OrthoDBiEOG7S226Z.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P46367-1 [UniParc]FASTAAdd to Basket

« Hide

MFSRSTLCLK TSASSIGRLQ LRYFSHLPMT VPIKLPNGLE YEQPTGLFIN    50
NKFVPSKQNK TFEVINPSTE EEICHIYEGR EDDVEEAVQA ADRAFSNGSW 100
NGIDPIDRGK ALYRLAELIE QDKDVIASIE TLDNGKAISS SRGDVDLVIN 150
YLKSSAGFAD KIDGRMIDTG RTHFSYTKRQ PLGVCGQIIP WNFPLLMWAW 200
KIAPALVTGN TVVLKTAEST PLSALYVSKY IPQAGIPPGV INIVSGFGKI 250
VGEAITNHPK IKKVAFTGST ATGRHIYQSA AAGLKKVTLE LGGKSPNIVF 300
ADAELKKAVQ NIILGIYYNS GEVCCAGSRV YVEESIYDKF IEEFKAASES 350
IKVGDPFDES TFQGAQTSQM QLNKILKYVD IGKNEGATLI TGGERLGSKG 400
YFIKPTVFGD VKEDMRIVKE EIFGPVVTVT KFKSADEVIN MANDSEYGLA 450
AGIHTSNINT ALKVADRVNA GTVWINTYND FHHAVPFGGF NASGLGREMS 500
VDALQNYLQV KAVRAKLDE 519
Length:519
Mass (Da):56,724
Last modified:November 1, 1997 - v2
Checksum:iE7D9944EA25F948E
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti51 – 511N → NN AA sequence 1 Publication
Sequence conflicti63 – 631E → V AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z75282 Genomic DNA. Translation: CAA99705.1.
BK006948 Genomic DNA. Translation: DAA11133.1.
PIRiS67286.
RefSeqiNP_015019.1. NM_001183794.1.

Genome annotation databases

EnsemblFungiiYOR374W; YOR374W; YOR374W.
GeneIDi854556.
KEGGisce:YOR374W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z75282 Genomic DNA. Translation: CAA99705.1 .
BK006948 Genomic DNA. Translation: DAA11133.1 .
PIRi S67286.
RefSeqi NP_015019.1. NM_001183794.1.

3D structure databases

ProteinModelPortali P46367.
SMRi P46367. Positions 47-507.
ModBasei Search...

Protein-protein interaction databases

BioGridi 34757. 24 interactions.
DIPi DIP-4053N.
IntActi P46367. 5 interactions.
MINTi MINT-539582.
STRINGi 4932.YOR374W.

Proteomic databases

MaxQBi P46367.
PaxDbi P46367.
PeptideAtlasi P46367.
PRIDEi P46367.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YOR374W ; YOR374W ; YOR374W .
GeneIDi 854556.
KEGGi sce:YOR374W.

Organism-specific databases

CYGDi YOR374w.
SGDi S000005901. ALD4.

Phylogenomic databases

eggNOGi COG1012.
GeneTreei ENSGT00740000115528.
HOGENOMi HOG000271505.
KOi K00128.
OMAi MACWKMA.
OrthoDBi EOG7S226Z.

Enzyme and pathway databases

BioCyci YEAST:YOR374W-MONOMER.

Miscellaneous databases

NextBioi 976985.

Gene expression databases

Genevestigatori P46367.

Family and domain databases

Gene3Di 3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProi IPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
[Graphical view ]
Pfami PF00171. Aldedh. 1 hit.
[Graphical view ]
SUPFAMi SSF53720. SSF53720. 1 hit.
PROSITEi PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Comparison of benzyl alcohol dehydrogenases and benzaldehyde dehydrogenases from the benzyl alcohol and mandelate pathways in Acinetobacter calcoaceticus and from the TOL-plasmid-encoded toluene pathway in Pseudomonas putida. N-terminal amino acid sequences, amino acid compositions and immunological cross-reactions."
    Chalmers R.M., Keen J.N., Fewson C.A.
    Biochem. J. 273:99-107(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 25-65.
  4. "Identification of two-dimensional gel electrophoresis resolved yeast proteins by matrix-assisted laser desorption ionization mass spectrometry."
    Larsson T., Norbeck J., Karlsson H., Karlsson K.-A., Blomberg A.
    Electrophoresis 18:418-423(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 25-34 AND 378-386.
  5. "Identification and disruption of the gene encoding the K(+)-activated acetaldehyde dehydrogenase of Saccharomyces cerevisiae."
    Tessier W.D., Meaden P.G., Dickinson F.M., Midgley M.
    FEMS Microbiol. Lett. 164:29-34(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION.
  6. "Molecular cloning, characterization, and potential roles of cytosolic and mitochondrial aldehyde dehydrogenases in ethanol metabolism in Saccharomyces cerevisiae."
    Wang X., Mann C.J., Bai Y., Ni L., Weiner H.
    J. Bacteriol. 180:822-830(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY.
  7. "Yeast mitochondrial dehydrogenases are associated in a supramolecular complex."
    Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N., Manon S., Schmitter J.-M.
    Biochemistry 40:9758-9769(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Functional analysis of the ALD gene family of Saccharomyces cerevisiae during anaerobic growth on glucose: the NADP+-dependent Ald6p and Ald5p isoforms play a major role in acetate formation."
    Saint-Prix F., Boenquist L., Dequin S.
    Microbiology 150:2209-2220(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase."
    Reinders J., Wagner K., Zahedi R.P., Stojanovski D., Eyrich B., van der Laan M., Rehling P., Sickmann A., Pfanner N., Meisinger C.
    Mol. Cell. Proteomics 6:1896-1906(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-216 AND SER-500, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-96 AND SER-500, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiALDH4_YEAST
AccessioniPrimary (citable) accession number: P46367
Secondary accession number(s): D6W367, Q08898
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1997
Last modified: June 11, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 22200 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3

Similar proteinsi