Reviewed, UniProtKB/Swiss-Prot P46367 (ALDH4_YEAST)

Names and origin

Protein names

Recommended name:
    Potassium-activated aldehyde dehydrogenase, mitochondrial
    EC=1.2.1.3
Alternative name(s):
    K(+)-activated acetaldehyde dehydrogenase
      Short name=K(+)-ACDH

Gene names

Name:	ALD4
Synonyms:	ALD7, ALDH2
Ordered Locus Names:	YOR374W
ORF Names:	O6730

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	519 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	An aldehyde + NAD(+) + H(2)O = an acid + NADH.
Subcellular location	Mitochondrion matrix.
Miscellaneous	Present with 22200 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	ethanol metabolic process Ref.4 Inferred from mutant phenotype. Source: SGD oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial nucleoid Inferred from direct assay. Source: SGD
Molecular function	aldehyde dehydrogenase (NAD) activity Ref.4 Inferred from electronic annotation. Source: EC aldehyde dehydrogenase [NAD(P)+] activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 24	24	Mitochondrion
	Chain	25 – 519	495	Potassium-activated aldehyde dehydrogenase, mitochondrial		PRO_0000007165
Regions
	Nucleotide binding	268 – 273	6	NAD By similarity
Sites
	Active site	290	1	Proton acceptor By similarity
	Active site	324	1	Nucleophile By similarity
	Site	192	1	Transition state stabilizer By similarity
Amino acid modifications
	Modified residue	216	1	Phosphothreonine
	Modified residue	500	1	Phosphoserine
Experimental info
	Sequence conflict	51	1	N → NN AA sequence Ref.2
	Sequence conflict	63	1	E → V AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P46367-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: E7D9944EA25F948E	FASTA	519	56,724
	10 20 30 40 50 60 MFSRSTLCLK TSASSIGRLQ LRYFSHLPMT VPIKLPNGLE YEQPTGLFIN NKFVPSKQNK 70 80 90 100 110 120 TFEVINPSTE EEICHIYEGR EDDVEEAVQA ADRAFSNGSW NGIDPIDRGK ALYRLAELIE 130 140 150 160 170 180 QDKDVIASIE TLDNGKAISS SRGDVDLVIN YLKSSAGFAD KIDGRMIDTG RTHFSYTKRQ 190 200 210 220 230 240 PLGVCGQIIP WNFPLLMWAW KIAPALVTGN TVVLKTAEST PLSALYVSKY IPQAGIPPGV 250 260 270 280 290 300 INIVSGFGKI VGEAITNHPK IKKVAFTGST ATGRHIYQSA AAGLKKVTLE LGGKSPNIVF 310 320 330 340 350 360 ADAELKKAVQ NIILGIYYNS GEVCCAGSRV YVEESIYDKF IEEFKAASES IKVGDPFDES 370 380 390 400 410 420 TFQGAQTSQM QLNKILKYVD IGKNEGATLI TGGERLGSKG YFIKPTVFGD VKEDMRIVKE 430 440 450 460 470 480 EIFGPVVTVT KFKSADEVIN MANDSEYGLA AGIHTSNINT ALKVADRVNA GTVWINTYND 490 500 510 FHHAVPFGGF NASGLGREMS VDALQNYLQV KAVRAKLDE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"Comparison of benzyl alcohol dehydrogenases and benzaldehyde dehydrogenases from the benzyl alcohol and mandelate pathways in Acinetobacter calcoaceticus and from the TOL-plasmid-encoded toluene pathway in Pseudomonas putida. N-terminal amino acid sequences, amino acid compositions and immunological cross-reactions." Chalmers R.M., Keen J.N., Fewson C.A. Biochem. J. 273:99-107(1991) [PubMed: 1989592] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-65.
[3]	"Identification of two-dimensional gel electrophoresis resolved yeast proteins by matrix-assisted laser desorption ionization mass spectrometry." Larsson T., Norbeck J., Karlsson H., Karlsson K.-A., Blomberg A. Electrophoresis 18:418-423(1997) [PubMed: 9150920] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-34 AND 378-386.
[4]	"Identification and disruption of the gene encoding the K(+)-activated acetaldehyde dehydrogenase of Saccharomyces cerevisiae." Tessier W.D., Meaden P.G., Dickinson F.M., Midgley M. FEMS Microbiol. Lett. 164:29-34(1998) [PubMed: 9675847] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION.
[5]	"Yeast mitochondrial dehydrogenases are associated in a supramolecular complex." Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N., Manon S., Schmitter J.-M. Biochemistry 40:9758-9769(2001) [PubMed: 11502169] [Abstract] Cited for: SUBCELLULAR LOCATION.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase." Reinders J., Wagner K., Zahedi R.P., Stojanovski D., Eyrich B., van der Laan M., Rehling P., Sickmann A., Pfanner N., Meisinger C. Mol. Cell. Proteomics 6:1896-1906(2007) [PubMed: 17761666] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-216 AND SER-500, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z75282 Genomic DNA. Translation: CAA99705.1.
PIR	S67286.
RefSeq	NP_015019.1.
3D structure databases
HSSP	HSSP built from PDB template 1BI9 based on UniProtKB Q63639.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4053N.
IntAct	P46367.
Proteomic databases
PeptideAtlas	P46367.
Genome annotation databases
Ensembl	YOR374W. Saccharomyces cerevisiae. [Contig view]
GeneID	854556.
GenomeReviews	Gene locus YOR374W in contig Y13140_GR.
KEGG	sce:YOR374W.
NMPDR	fig|4932.3.peg.6138.
Organism-specific databases
CYGD	YOR374w.
SGD	S000005901. ALD4.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P46367.
Gene expression databases
GermOnline	YOR374W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR016160. Ald_DHase_CS. IPR016162. Ald_DHase_N. IPR015590. Aldehyde_DHase. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
LinkHub	P46367.
NextBio	976985.

Entry information

Entry name

ALDH4_YEAST

Accession

Primary (citable) accession number: P46367
Secondary accession number(s): Q08898

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1997
Last modified:	November 25, 2008
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)