Reviewed, UniProtKB/Swiss-Prot P46363 (BIRA_HAEIN)

Names and origin

Protein names

Recommended name:
    Bifunctional protein birA
Including the following 2 domains:
    1- Recommended name:
            Biotin operon repressor
    2- Recommended name:
            Biotin--[acetyl-CoA-carboxylase] synthetase
              EC=6.3.4.15
        Alternative name(s):
            Biotin--protein ligase

Gene names

Name:	birA
Ordered Locus Names:	HI0220.1

Organism

Haemophilus influenzae [Complete proteome] [HAMAP]

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	302 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'-adenylate and transfers the biotin moiety to biotin-accepting proteins By similarity.
Catalytic activity	ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)].
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the biotin--protein ligase family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	ATP-binding Biotin DNA-binding Nucleotide-binding
Molecular function	Ligase Repressor
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein modification process Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW biotin-[acetyl-CoA-carboxylase] ligase activity Inferred from electronic annotation. Source: EC transcription repressor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 302	302	Bifunctional protein birA		PRO_0000064933

Sequences

	Sequence		Length	Mass (Da)
	P46363-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 50EE9D6E9820378B	FASTA	302	34,342
	10 20 30 40 50 60 MMNFTLLTYL ADCQPKVRSE LEKFSKNLEE DIQQLREIGL DILVDGQDYR LVPMLPLLNP 70 80 90 100 110 120 QQISTALFPY SIHYQPIISS TNEWILQNIL SLKKGDLCVA EYQTAGRGRR GRQWLSPFAG 130 140 150 160 170 180 QIMFSFYWAF DPKKSIEGLS LVIGLAIAEV LNVQVKWPND ILFDERKLGG ILVEIANHKN 190 200 210 220 230 240 GMLNLVIGIG INVSLSKQTE ISQPYAEVCE IDPDVERQTL LPKLIQHLYT RLNIFEQNGI 250 260 270 280 290 300 DEEFQQAWQS YNAFSNSEIN VLTEQGVISG IEQGIDERGY LKVLCGNKIQ MFNGGEVSLR KK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
[2]	Koonin E.V., Rudd K.E. Submitted (SEP-1995) to UniProtKB Cited for: IDENTIFICATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L42023 Genomic DNA. Translation: AAC21889.1.
RefSeq	NP_438391.1.
3D structure databases
HSSP	HSSP built from PDB template 1BIA based on UniProtKB P06709.
ModBase	Search...
Genome annotation databases
GeneID	951136.
GenomeReviews	Gene locus HI0220.1 in contig L42023_GR.
KEGG	hin:HI0220.1.
NMPDR	fig|71421.1.peg.209.
TIGR	HI0220.1.
Phylogenomic databases
HOGENOM	P46363.
OMA	ISSTNEW.
Enzyme and pathway databases
BioCyc	HINF71421:HI_0220.1-MON.
BRENDA	6.3.4.15. 109.
Family and domain databases
InterPro	IPR004408. Biotin_CoA_COase_ligase. IPR003142. BPL_C. IPR004143. BPL_LipA_LipB. IPR004409. HTHbiotin_repres. [Graphical view]
PANTHER	PTHR12835. BirA_ligase. 1 hit.
Pfam	PF02237. BPL_C. 1 hit. PF03099. BPL_LipA_LipB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00121. birA_ligase. 1 hit. TIGR00122. birA_repr_reg. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIRA_HAEIN

Accession

Primary (citable) accession number: P46363

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 3, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)