P46354 (PUNA_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Purine nucleoside phosphorylase 1
Short name=PNP 1
EC=2.4.2.1

Alternative name(s):
Inosine phosphorylase
Inosine-guanosine phosphorylase
Purine nucleoside phosphorylase I
Short name=PNP I
Short name=PU-NPase I

Gene names

Name:	punA
Synonyms:	deoD, pnp, yqkO
Ordered Locus Names:	BSU23490

Organism

Bacillus subtilis (strain 168) [Reference proteome] [HAMAP]

Taxonomic identifier

224308 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus subtilis group › Bacillus subtilis › Bacillus subtilis subsp. subtilis

Protein attributes

Sequence length	271 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate. Cleaves guanosine and inosine.
Catalytic activity	Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate.
Pathway	Purine metabolism; purine nucleoside salvage.
Sequence similarities	Belongs to the PNP/MTAP phosphorylase family.

Ontologies

Keywords
Molecular function	Glycosyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	purine-nucleoside phosphorylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 271	271	Purine nucleoside phosphorylase 1		PRO_0000184540
Amino acid modifications
	Modified residue	28	1	Phosphoserine Ref.5
Experimental info
	Sequence conflict	184	1	A → D in M85047. Ref.4
	Sequence conflict	227	1	G → A in M85047. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P46354 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 420B26E7309D9FC9	FASTA	271	29,127
	10 20 30 40 50 60 MKDRIERAAA FIKQNLPESP KIGLILGSGL GILADEIENP VKLKYEDIPE FPVSTVEGHA 70 80 90 100 110 120 GQLVLGTLEG VSVIAMQGRF HFYEGYSMEK VTFPVRVMKA LGVEALIVTN AAGGVNTEFR 130 140 150 160 170 180 AGDLMIITDH INFMGTNPLI GPNEADFGAR FPDMSSAYDK DLSSLAEKIA KDLNIPIQKG 190 200 210 220 230 240 VYTAVTGPSY ETPAEVRFLR TMGSDAVGMS TVPEVIVANH AGMRVLGISC ISNAAAGILD 250 260 270 QPLSHDEVME VTEKVKAGFL KLVKAIVAQY E

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleosides as a carbon source in Bacillus subtilis: characterization of the drm-pupG operon." Schuch R., Garibian A., Saxild H.H., Piggot P.J., Nygaard P. Microbiology 145:2957-2966(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / BR151.
[2]	"Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes." Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M., Kobayashi Y. Microbiology 142:3103-3111(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / JH642.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[4]	"Characterization of a Bacillus subtilis sporulation operon that includes genes for an RNA polymerase sigma factor and for a putative DD-carboxypeptidase." Wu J.-J., Schuch R., Piggot P.J. J. Bacteriol. 174:4885-4892(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 161-271. Strain: 168 / MB24.
[5]	"The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis." Macek B., Mijakovic I., Olsen J.V., Gnad F., Kumar C., Jensen P.R., Mann M. Mol. Cell. Proteomics 6:697-707(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-28, MASS SPECTROMETRY. Strain: 168.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U32685 Genomic DNA. Translation: AAA74434.1. D84432 Genomic DNA. Translation: BAA12651.1. AL009126 Genomic DNA. Translation: CAB14281.1. M85047 Genomic DNA. No translation available.
PIR	D69680.
RefSeq	NP_390230.1. NC_000964.3.
3D structure databases
ProteinModelPortal	P46354.
SMR	P46354. Positions 1-270.
ModBase	Search...
Protein-protein interaction databases
STRING	224308.BSU23490.
PTM databases
PhosSite	P0802232.
Proteomic databases
PaxDb	P46354.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAB14281; CAB14281; BSU23490.
GeneID	938731.
KEGG	bsu:BSU23490.
PATRIC	18976517. VBIBacSub10457_2449.
Organism-specific databases
GenoList	BSU23490. [Micado]
Phylogenomic databases
eggNOG	COG0005.
HOGENOM	HOG000045183.
KO	K03783.
OMA	MKVMTTP.
ProtClustDB	PRK08202.
Enzyme and pathway databases
BioCyc	BSUB:BSU23490-MONOMER.
UniPathway	UPA00606.
Family and domain databases
InterPro	IPR000845. Nucleoside_phosphorylase_d. IPR001369. PNP/MTAP. IPR011270. Pur_Nuc_Pase_Ino/Guo-sp. IPR011268. Purine_phosphorylase. IPR018099. Purine_phosphorylase-2_CS. [Graphical view]
PANTHER	PTHR11904. PTHR11904. 1 hit. PTHR11904:SF9. PTHR11904:SF9. 1 hit.
Pfam	PF01048. PNP_UDP_1. 1 hit. [Graphical view]
PIRSF	PIRSF000477. PurNPase. 1 hit.
TIGRFAMs	TIGR01700. PNPH. 1 hit. TIGR01697. PNPH-PUNA-XAPA. 1 hit.
PROSITE	PS01240. PNP_MTAP_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUNA_BACSU

Accession

Primary (citable) accession number: P46354

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program