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Protein

Purine nucleoside phosphorylase 1

Gene

punA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate. Cleaves guanosine, inosine, 2'-deoxyguanosine and 2'-deoxyinosine.By similarity

Catalytic activityi

Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate.By similarity

Pathwayi: purine nucleoside salvage

This protein is involved in the pathway purine nucleoside salvage, which is part of Purine metabolism.
View all proteins of this organism that are known to be involved in the pathway purine nucleoside salvage and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei28PhosphateBy similarity1
Binding sitei59PhosphateBy similarity1
Binding sitei111Phosphate; via amide nitrogenBy similarity1
Binding sitei191Purine nucleosideBy similarity1
Binding sitei210PhosphateBy similarity1
Binding sitei233Purine nucleosideBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciBSUB:BSU23490-MONOMER.
BRENDAi2.4.2.1. 658.
UniPathwayiUPA00606.

Names & Taxonomyi

Protein namesi
Recommended name:
Purine nucleoside phosphorylase 1 (EC:2.4.2.1)
Short name:
PNP 1
Alternative name(s):
Inosine phosphorylase
Inosine-guanosine phosphorylase
Purine nucleoside phosphorylase I
Short name:
PNP I
Short name:
Pu-NPase I
Gene namesi
Name:punA
Synonyms:deoD, pnp, yqkO
Ordered Locus Names:BSU23490
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001845401 – 271Purine nucleoside phosphorylase 1Add BLAST271

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei28Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP46354.
PRIDEiP46354.

PTM databases

iPTMnetiP46354.

Interactioni

Subunit structurei

Homotrimer.By similarity

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100012891.

Structurei

3D structure databases

ProteinModelPortaliP46354.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni79 – 81Phosphate bindingBy similarity3

Sequence similaritiesi

Belongs to the PNP/MTAP phosphorylase family.Curated

Phylogenomic databases

eggNOGiENOG4105CB1. Bacteria.
COG0005. LUCA.
HOGENOMiHOG000045183.
InParanoidiP46354.
KOiK03783.
OMAiMENGYTY.
PhylomeDBiP46354.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
InterProiIPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR011270. Pur_Nuc_Pase_Ino/Guo-sp.
IPR011268. Purine_phosphorylase.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PTHR11904:SF9. PTHR11904:SF9. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000477. PurNPase. 1 hit.
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01700. PNPH. 1 hit.
TIGR01697. PNPH-PUNA-XAPA. 1 hit.
PROSITEiPS01240. PNP_MTAP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P46354-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKDRIERAAA FIKQNLPESP KIGLILGSGL GILADEIENP VKLKYEDIPE
60 70 80 90 100
FPVSTVEGHA GQLVLGTLEG VSVIAMQGRF HFYEGYSMEK VTFPVRVMKA
110 120 130 140 150
LGVEALIVTN AAGGVNTEFR AGDLMIITDH INFMGTNPLI GPNEADFGAR
160 170 180 190 200
FPDMSSAYDK DLSSLAEKIA KDLNIPIQKG VYTAVTGPSY ETPAEVRFLR
210 220 230 240 250
TMGSDAVGMS TVPEVIVANH AGMRVLGISC ISNAAAGILD QPLSHDEVME
260 270
VTEKVKAGFL KLVKAIVAQY E
Length:271
Mass (Da):29,127
Last modified:November 1, 1995 - v1
Checksum:i420B26E7309D9FC9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti184A → D in M85047 (PubMed:1629150).Curated1
Sequence conflicti227G → A in M85047 (PubMed:1629150).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U32685 Genomic DNA. Translation: AAA74434.1.
D84432 Genomic DNA. Translation: BAA12651.1.
AL009126 Genomic DNA. Translation: CAB14281.1.
M85047 Genomic DNA. No translation available.
PIRiD69680.
RefSeqiNP_390230.1. NC_000964.3.
WP_003230447.1. NZ_JNCM01000036.1.

Genome annotation databases

EnsemblBacteriaiCAB14281; CAB14281; BSU23490.
GeneIDi938731.
KEGGibsu:BSU23490.
PATRICi18976517. VBIBacSub10457_2449.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U32685 Genomic DNA. Translation: AAA74434.1.
D84432 Genomic DNA. Translation: BAA12651.1.
AL009126 Genomic DNA. Translation: CAB14281.1.
M85047 Genomic DNA. No translation available.
PIRiD69680.
RefSeqiNP_390230.1. NC_000964.3.
WP_003230447.1. NZ_JNCM01000036.1.

3D structure databases

ProteinModelPortaliP46354.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100012891.

PTM databases

iPTMnetiP46354.

Proteomic databases

PaxDbiP46354.
PRIDEiP46354.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB14281; CAB14281; BSU23490.
GeneIDi938731.
KEGGibsu:BSU23490.
PATRICi18976517. VBIBacSub10457_2449.

Phylogenomic databases

eggNOGiENOG4105CB1. Bacteria.
COG0005. LUCA.
HOGENOMiHOG000045183.
InParanoidiP46354.
KOiK03783.
OMAiMENGYTY.
PhylomeDBiP46354.

Enzyme and pathway databases

UniPathwayiUPA00606.
BioCyciBSUB:BSU23490-MONOMER.
BRENDAi2.4.2.1. 658.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
InterProiIPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR011270. Pur_Nuc_Pase_Ino/Guo-sp.
IPR011268. Purine_phosphorylase.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PTHR11904:SF9. PTHR11904:SF9. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000477. PurNPase. 1 hit.
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01700. PNPH. 1 hit.
TIGR01697. PNPH-PUNA-XAPA. 1 hit.
PROSITEiPS01240. PNP_MTAP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPUNA_BACSU
AccessioniPrimary (citable) accession number: P46354
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.