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P46350 (RXL7_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribosome-associated protein L7Ae-like
Gene names
Name:rplGB
Synonyms:ybaB, ybxF
Ordered Locus Names:BSU01090
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length82 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

RNA-binding protein that recognizes the K-turn motif present in ribosomal RNA, but also in box C/D and box C'/D' sRNAs. Ref.5

Subunit structure

Found associated with the 50S subunit of the ribosome during exponential growth but not during stationary phase.

Subcellular location

Cytoplasm. Note: Localizes to the cell pole in exponential growth. HAMAP-Rule MF_00574

Disruption phenotype

None; has no effect on viability, growth or sporulation at low, normal or elevated temperatures. Ref.4

Sequence similarities

Belongs to the ribosomal protein L7Ae family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandRNA-binding
   Molecular functionRibonucleoprotein
Ribosomal protein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtranslation

Inferred from electronic annotation. Source: InterPro

   Cellular_componentribosome

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

structural constituent of ribosome

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 8282Ribosome-associated protein L7Ae-like HAMAP-Rule MF_00574
PRO_0000136812

Experimental info

Mutagenesis171K → A: No effect. Ref.4
Mutagenesis211K → A: No effect. Ref.4
Mutagenesis241K → A: Decreased ribosomal association. Ref.4

Secondary structure

................. 82
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P46350 [UniParc].

Last modified July 26, 2002. Version 3.
Checksum: B8E301C141E77211

FASTA828,462
        10         20         30         40         50         60 
MSYDKVSQAK SIIIGTKQTV KALKRGSVKE VVVAKDADPI LTSSVVSLAE DQGISVSMVE 

        70         80 
SMKKLGKACG IEVGAAAVAI IL 

« Hide

References

« Hide 'large scale' references
[1]"Genetic and transcriptional organization of the region encoding the beta subunit of Bacillus subtilis RNA polymerase."
Boor K.J., Duncan M.L., Price C.W.
J. Biol. Chem. 270:20329-20336(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
[2]"Sequence analysis of a 50 kb region between spo0H and rrnH on the Bacillus subtilis chromosome."
Yasumoto K., Liu H., Jeong S.M., Ohashi Y., Kakinuma S., Tanaka K., Kawamura F., Yoshikawa H., Takahashi H.
Microbiology 142:3039-3046(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[3]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[4]"YbxF, a protein associated with exponential-phase ribosomes in Bacillus subtilis."
Sojka L., Fucik V., Krasny L., Barvik I., Jonak J.
J. Bacteriol. 189:4809-4814(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: RIBOSOME ASSOCIATION, DISRUPTION PHENOTYPE, MUTAGENESIS OF LYS-17; LYS-21 AND LYS-24.
Strain: 168.
[5]"YbxF and YlxQ are bacterial homologs of L7Ae and bind K-turns but not K-loops."
Baird N.J., Zhang J., Hamma T., Ferre-D'Amare A.R.
RNA 18:759-770(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) IN COMPLEX WITH RNA, FUNCTION, RNA-BINDING.
[6]"Co-crystal structure of a T-box riboswitch stem I domain in complex with its cognate tRNA."
Zhang J., Ferre-D'Amare A.R.
Nature 500:363-366(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS) IN COMPLEX WITH K-TURN RNA.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L43593 Genomic DNA. Translation: AAB59113.1.
D64127 Genomic DNA. Translation: BAA11000.1.
AL009126 Genomic DNA. Translation: CAB11885.1.
PIRG69751.
RefSeqNP_387990.1. NC_000964.3.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3V7EX-ray2.80A/B2-82[»]
4LCKX-ray3.20A/D2-82[»]
ProteinModelPortalP46350.
SMRP46350. Positions 2-81.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224308.BSU01090.

Proteomic databases

PaxDbP46350.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB11885; CAB11885; BSU01090.
GeneID936829.
KEGGbsu:BSU01090.
PATRIC18971727. VBIBacSub10457_0112.

Organism-specific databases

GenoListBSU01090. [Micado]

Phylogenomic databases

eggNOGCOG1358.
HOGENOMHOG000055225.
KOK07590.
OMALMELICW.
OrthoDBEOG6B8XRH.

Enzyme and pathway databases

BioCycBSUB:BSU01090-MONOMER.

Family and domain databases

Gene3D3.30.1330.30. 1 hit.
HAMAPMF_00574. Ribosomal_L7Ae_Bact.
InterProIPR029064. L30e-like.
IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
IPR000948. Ribosomal_L7Ae_prok.
IPR023460. Ribosomal_L7Ae_putative.
[Graphical view]
PfamPF01248. Ribosomal_L7Ae. 1 hit.
[Graphical view]
PRINTSPR00884. RIBOSOMALHS6.
SUPFAMSSF55315. SSF55315. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRXL7_BACSU
AccessionPrimary (citable) accession number: P46350
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: July 26, 2002
Last modified: June 11, 2014
This is version 102 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Ribosomal proteins

Ribosomal proteins families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList