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Protein

Ribosome-associated protein L7Ae-like

Gene

rplGB

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

RNA-binding protein that recognizes the K-turn motif present in ribosomal RNA, but also in box C/D and box C'/D' sRNAs.1 Publication

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
  2. structural constituent of ribosome Source: InterPro

GO - Biological processi

  1. translation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciBSUB:BSU01090-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosome-associated protein L7Ae-like
Gene namesi
Name:rplGB
Synonyms:ybaB, ybxF
Ordered Locus Names:BSU01090
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU01090. [Micado]

Subcellular locationi

Cytoplasm
Note: Localizes to the cell pole in exponential growth.

GO - Cellular componenti

  1. ribosome Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

None; has no effect on viability, growth or sporulation at low, normal or elevated temperatures.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi17 – 171K → A: No effect. 1 Publication
Mutagenesisi21 – 211K → A: No effect. 1 Publication
Mutagenesisi24 – 241K → A: Decreased ribosomal association. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 8282Ribosome-associated protein L7Ae-likePRO_0000136812Add
BLAST

Proteomic databases

PaxDbiP46350.

Interactioni

Subunit structurei

Found associated with the 50S subunit of the ribosome during exponential growth but not during stationary phase.2 Publications

Protein-protein interaction databases

STRINGi224308.BSU01090.

Structurei

Secondary structure

1
82
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 86Combined sources
Beta strandi10 – 156Combined sources
Helixi16 – 238Combined sources
Turni24 – 263Combined sources
Beta strandi28 – 347Combined sources
Helixi39 – 5214Combined sources
Beta strandi56 – 605Combined sources
Helixi62 – 687Combined sources
Beta strandi76 – 816Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3V7EX-ray2.80A/B2-82[»]
4LCKX-ray3.20A/D2-82[»]
4TZPX-ray8.50A/D2-82[»]
4TZVX-ray5.03A2-82[»]
4TZWX-ray4.67A2-82[»]
4TZZX-ray3.64A/D/G/J2-82[»]
ProteinModelPortaliP46350.
SMRiP46350. Positions 2-81.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein L7Ae family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1358.
HOGENOMiHOG000055225.
InParanoidiP46350.
KOiK07590.
OMAiMSYEKVA.
OrthoDBiEOG6B8XRH.

Family and domain databases

Gene3Di3.30.1330.30. 1 hit.
HAMAPiMF_00574. Ribosomal_L7Ae_Bact.
InterProiIPR029064. L30e-like.
IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
IPR000948. Ribosomal_L7Ae_prok.
IPR023460. Ribosomal_L7Ae_putative.
[Graphical view]
PfamiPF01248. Ribosomal_L7Ae. 1 hit.
[Graphical view]
PRINTSiPR00884. RIBOSOMALHS6.
SUPFAMiSSF55315. SSF55315. 1 hit.

Sequencei

Sequence statusi: Complete.

P46350-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYDKVSQAK SIIIGTKQTV KALKRGSVKE VVVAKDADPI LTSSVVSLAE
60 70 80
DQGISVSMVE SMKKLGKACG IEVGAAAVAI IL
Length:82
Mass (Da):8,462
Last modified:July 26, 2002 - v3
Checksum:iB8E301C141E77211
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43593 Genomic DNA. Translation: AAB59113.1.
D64127 Genomic DNA. Translation: BAA11000.1.
AL009126 Genomic DNA. Translation: CAB11885.1.
PIRiG69751.
RefSeqiNP_387990.1. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB11885; CAB11885; BSU01090.
GeneIDi936829.
KEGGibsu:BSU01090.
PATRICi18971727. VBIBacSub10457_0112.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43593 Genomic DNA. Translation: AAB59113.1.
D64127 Genomic DNA. Translation: BAA11000.1.
AL009126 Genomic DNA. Translation: CAB11885.1.
PIRiG69751.
RefSeqiNP_387990.1. NC_000964.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3V7EX-ray2.80A/B2-82[»]
4LCKX-ray3.20A/D2-82[»]
4TZPX-ray8.50A/D2-82[»]
4TZVX-ray5.03A2-82[»]
4TZWX-ray4.67A2-82[»]
4TZZX-ray3.64A/D/G/J2-82[»]
ProteinModelPortaliP46350.
SMRiP46350. Positions 2-81.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.BSU01090.

Proteomic databases

PaxDbiP46350.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB11885; CAB11885; BSU01090.
GeneIDi936829.
KEGGibsu:BSU01090.
PATRICi18971727. VBIBacSub10457_0112.

Organism-specific databases

GenoListiBSU01090. [Micado]

Phylogenomic databases

eggNOGiCOG1358.
HOGENOMiHOG000055225.
InParanoidiP46350.
KOiK07590.
OMAiMSYEKVA.
OrthoDBiEOG6B8XRH.

Enzyme and pathway databases

BioCyciBSUB:BSU01090-MONOMER.

Family and domain databases

Gene3Di3.30.1330.30. 1 hit.
HAMAPiMF_00574. Ribosomal_L7Ae_Bact.
InterProiIPR029064. L30e-like.
IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
IPR000948. Ribosomal_L7Ae_prok.
IPR023460. Ribosomal_L7Ae_putative.
[Graphical view]
PfamiPF01248. Ribosomal_L7Ae. 1 hit.
[Graphical view]
PRINTSiPR00884. RIBOSOMALHS6.
SUPFAMiSSF55315. SSF55315. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genetic and transcriptional organization of the region encoding the beta subunit of Bacillus subtilis RNA polymerase."
    Boor K.J., Duncan M.L., Price C.W.
    J. Biol. Chem. 270:20329-20336(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
  2. "Sequence analysis of a 50 kb region between spo0H and rrnH on the Bacillus subtilis chromosome."
    Yasumoto K., Liu H., Jeong S.M., Ohashi Y., Kakinuma S., Tanaka K., Kawamura F., Yoshikawa H., Takahashi H.
    Microbiology 142:3039-3046(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  4. "YbxF, a protein associated with exponential-phase ribosomes in Bacillus subtilis."
    Sojka L., Fucik V., Krasny L., Barvik I., Jonak J.
    J. Bacteriol. 189:4809-4814(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: RIBOSOME ASSOCIATION, DISRUPTION PHENOTYPE, MUTAGENESIS OF LYS-17; LYS-21 AND LYS-24.
    Strain: 168.
  5. "YbxF and YlxQ are bacterial homologs of L7Ae and bind K-turns but not K-loops."
    Baird N.J., Zhang J., Hamma T., Ferre-D'Amare A.R.
    RNA 18:759-770(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) IN COMPLEX WITH RNA, FUNCTION, RNA-BINDING.
  6. "Co-crystal structure of a T-box riboswitch stem I domain in complex with its cognate tRNA."
    Zhang J., Ferre-D'Amare A.R.
    Nature 500:363-366(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS) IN COMPLEX WITH K-TURN RNA.

Entry informationi

Entry nameiRXL7_BACSU
AccessioniPrimary (citable) accession number: P46350
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: July 26, 2002
Last modified: January 7, 2015
This is version 106 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Ribosomal proteins
    Ribosomal proteins families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.