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P46350

- RXL7_BACSU

UniProt

P46350 - RXL7_BACSU

Protein

Ribosome-associated protein L7Ae-like

Gene

rplGB

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    RNA-binding protein that recognizes the K-turn motif present in ribosomal RNA, but also in box C/D and box C'/D' sRNAs.1 Publication

    GO - Molecular functioni

    1. RNA binding Source: UniProtKB-KW
    2. structural constituent of ribosome Source: InterPro

    GO - Biological processi

    1. translation Source: InterPro

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Keywords - Ligandi

    RNA-binding

    Enzyme and pathway databases

    BioCyciBSUB:BSU01090-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribosome-associated protein L7Ae-like
    Gene namesi
    Name:rplGB
    Synonyms:ybaB, ybxF
    Ordered Locus Names:BSU01090
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU01090. [Micado]

    Subcellular locationi

    Cytoplasm
    Note: Localizes to the cell pole in exponential growth.

    GO - Cellular componenti

    1. ribosome Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    None; has no effect on viability, growth or sporulation at low, normal or elevated temperatures.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi17 – 171K → A: No effect. 1 Publication
    Mutagenesisi21 – 211K → A: No effect. 1 Publication
    Mutagenesisi24 – 241K → A: Decreased ribosomal association. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 8282Ribosome-associated protein L7Ae-likePRO_0000136812Add
    BLAST

    Proteomic databases

    PaxDbiP46350.

    Interactioni

    Subunit structurei

    Found associated with the 50S subunit of the ribosome during exponential growth but not during stationary phase.2 Publications

    Protein-protein interaction databases

    STRINGi224308.BSU01090.

    Structurei

    Secondary structure

    1
    82
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi3 – 86
    Beta strandi10 – 156
    Helixi16 – 238
    Turni24 – 263
    Beta strandi28 – 347
    Helixi39 – 5214
    Beta strandi56 – 605
    Helixi62 – 687
    Beta strandi76 – 816

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3V7EX-ray2.80A/B2-82[»]
    4LCKX-ray3.20A/D2-82[»]
    ProteinModelPortaliP46350.
    SMRiP46350. Positions 2-81.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribosomal protein L7Ae family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1358.
    HOGENOMiHOG000055225.
    KOiK07590.
    OMAiLMELICW.
    OrthoDBiEOG6B8XRH.

    Family and domain databases

    Gene3Di3.30.1330.30. 1 hit.
    HAMAPiMF_00574. Ribosomal_L7Ae_Bact.
    InterProiIPR029064. L30e-like.
    IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
    IPR000948. Ribosomal_L7Ae_prok.
    IPR023460. Ribosomal_L7Ae_putative.
    [Graphical view]
    PfamiPF01248. Ribosomal_L7Ae. 1 hit.
    [Graphical view]
    PRINTSiPR00884. RIBOSOMALHS6.
    SUPFAMiSSF55315. SSF55315. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P46350-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSYDKVSQAK SIIIGTKQTV KALKRGSVKE VVVAKDADPI LTSSVVSLAE   50
    DQGISVSMVE SMKKLGKACG IEVGAAAVAI IL 82
    Length:82
    Mass (Da):8,462
    Last modified:July 26, 2002 - v3
    Checksum:iB8E301C141E77211
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L43593 Genomic DNA. Translation: AAB59113.1.
    D64127 Genomic DNA. Translation: BAA11000.1.
    AL009126 Genomic DNA. Translation: CAB11885.1.
    PIRiG69751.
    RefSeqiNP_387990.1. NC_000964.3.
    WP_003225778.1. NZ_CM000487.1.

    Genome annotation databases

    EnsemblBacteriaiCAB11885; CAB11885; BSU01090.
    GeneIDi936829.
    KEGGibsu:BSU01090.
    PATRICi18971727. VBIBacSub10457_0112.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L43593 Genomic DNA. Translation: AAB59113.1 .
    D64127 Genomic DNA. Translation: BAA11000.1 .
    AL009126 Genomic DNA. Translation: CAB11885.1 .
    PIRi G69751.
    RefSeqi NP_387990.1. NC_000964.3.
    WP_003225778.1. NZ_CM000487.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3V7E X-ray 2.80 A/B 2-82 [» ]
    4LCK X-ray 3.20 A/D 2-82 [» ]
    ProteinModelPortali P46350.
    SMRi P46350. Positions 2-81.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU01090.

    Proteomic databases

    PaxDbi P46350.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB11885 ; CAB11885 ; BSU01090 .
    GeneIDi 936829.
    KEGGi bsu:BSU01090.
    PATRICi 18971727. VBIBacSub10457_0112.

    Organism-specific databases

    GenoListi BSU01090. [Micado ]

    Phylogenomic databases

    eggNOGi COG1358.
    HOGENOMi HOG000055225.
    KOi K07590.
    OMAi LMELICW.
    OrthoDBi EOG6B8XRH.

    Enzyme and pathway databases

    BioCyci BSUB:BSU01090-MONOMER.

    Family and domain databases

    Gene3Di 3.30.1330.30. 1 hit.
    HAMAPi MF_00574. Ribosomal_L7Ae_Bact.
    InterProi IPR029064. L30e-like.
    IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
    IPR000948. Ribosomal_L7Ae_prok.
    IPR023460. Ribosomal_L7Ae_putative.
    [Graphical view ]
    Pfami PF01248. Ribosomal_L7Ae. 1 hit.
    [Graphical view ]
    PRINTSi PR00884. RIBOSOMALHS6.
    SUPFAMi SSF55315. SSF55315. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genetic and transcriptional organization of the region encoding the beta subunit of Bacillus subtilis RNA polymerase."
      Boor K.J., Duncan M.L., Price C.W.
      J. Biol. Chem. 270:20329-20336(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
    2. "Sequence analysis of a 50 kb region between spo0H and rrnH on the Bacillus subtilis chromosome."
      Yasumoto K., Liu H., Jeong S.M., Ohashi Y., Kakinuma S., Tanaka K., Kawamura F., Yoshikawa H., Takahashi H.
      Microbiology 142:3039-3046(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    4. "YbxF, a protein associated with exponential-phase ribosomes in Bacillus subtilis."
      Sojka L., Fucik V., Krasny L., Barvik I., Jonak J.
      J. Bacteriol. 189:4809-4814(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: RIBOSOME ASSOCIATION, DISRUPTION PHENOTYPE, MUTAGENESIS OF LYS-17; LYS-21 AND LYS-24.
      Strain: 168.
    5. "YbxF and YlxQ are bacterial homologs of L7Ae and bind K-turns but not K-loops."
      Baird N.J., Zhang J., Hamma T., Ferre-D'Amare A.R.
      RNA 18:759-770(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) IN COMPLEX WITH RNA, FUNCTION, RNA-BINDING.
    6. "Co-crystal structure of a T-box riboswitch stem I domain in complex with its cognate tRNA."
      Zhang J., Ferre-D'Amare A.R.
      Nature 500:363-366(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS) IN COMPLEX WITH K-TURN RNA.

    Entry informationi

    Entry nameiRXL7_BACSU
    AccessioniPrimary (citable) accession number: P46350
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: July 26, 2002
    Last modified: October 1, 2014
    This is version 104 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. Ribosomal proteins
      Ribosomal proteins families and list of entries
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3