Reviewed, UniProtKB/Swiss-Prot P46321 (LICR_BACSU)

Names and origin

Protein names

Recommended name:
    Probable licABCH operon regulator
Including the following 2 domains:
    1- Recommended name:
            Putative phosphotransferase enzyme IIB component
              EC=2.7.1.69
        Alternative name(s):
            Putative PTS system EIIB component
    2- Recommended name:
            Putative phosphotransferase enzyme IIA component
              EC=2.7.1.-
        Alternative name(s):
            Putative PTS system EIIA component

Gene names

Name:	licR
Synonyms:	celR
Ordered Locus Names:	BSU38600

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	641 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Positive regulator of the licABCH operon.
Catalytic activity	Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine. Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Domain	The PTS EIIB type-2 domain may serve a regulatory function, through its phosphorylation activity. The PTS EIIA type-2 domain may serve a regulatory function, through its phosphorylation activity.
Miscellaneous	Might be activated by phosphorylation of certain histidyl residues by HPr.
Sequence similarities	Belongs to the transcriptional antiterminator bglG family. Contains 2 PRD domains. Contains 1 PTS EIIA type-2 domain. Contains 1 PTS EIIB type-2 domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Domain	Repeat
Ligand	RNA-binding
Molecular function	Activator Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 641	641	Probable licABCH operon regulator		PRO_0000204245
Regions
	Domain	184 – 289	106	PRD 1
	Domain	296 – 403	108	PRD 2
	Domain	407 – 498	92	PTS EIIB type-2
	Domain	499 – 638	140	PTS EIIA type-2
Amino acid modifications
	Modified residue	219	1	Phosphohistidine Potential
	Modified residue	278	1	Phosphohistidine Potential
	Modified residue	333	1	Phosphohistidine Potential
	Modified residue	392	1	Phosphohistidine Potential
	Modified residue	413	1	Phosphocysteine; by EIIA By similarity
	Modified residue	559	1	Phosphohistidine; by HPr By similarity
Experimental info
	Mutagenesis	219	1	H → A: Residual activity. Ref.4
	Mutagenesis	219	1	H → D: Loss of activity. Ref.4
	Mutagenesis	278	1	H → A: Loss of activity. Ref.4
	Mutagenesis	278	1	H → E: Loss of activity. Ref.4
	Mutagenesis	333	1	H → A: Loss of activity. Ref.4
	Mutagenesis	392	1	H → E: Loss of activity. Ref.4
	Mutagenesis	392	1	H → I: Residual activity. Ref.4
	Mutagenesis	559	1	H → G: Increase in activity. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P46321-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: FCEF83BFC72A0168	FASTA	641	73,315
	10 20 30 40 50 60 MLHGRLRDIL RLLMAAEAPV TSSFFAAQLN VTTRTVRNDI KELQGVLSGH GAFVQSVRGS 70 80 90 100 110 120 GYKLRIDDEQ VFRTLLQDEF QQKKGLPVLP EERMAYLMKR LLLADHYLKL DELAEELFIS 130 140 150 160 170 180 KSTLQTDLKE VKKRLLPYRI VMETRPNYGF KLRGDEVQMR YCMAEYIVDE RETEIDVLNE 190 200 210 220 230 240 KADILPKEEI EIIRSAILKK MKNDRIPLSN MGLNNLIIHI AIACKRIRTE NYVSLFPKDM 250 260 270 280 290 300 DHILHQKEYQ AAEAIVKELE SKLSVTFPKD ETAYITMHLL GTKRMTQSQC GEDTFSIEEE 310 320 330 340 350 360 TDQLTLAMIK AVDRELKLGI LHDKELKIGL ALHMKPAISR NRYGMNLRNP MLAAIKEHYP 370 380 390 400 410 420 LAFEAGIIAG IVIKEQTGIE IHENEIGYLA LHFGAAIERK KTESPPKRCI IVCASGAGSA 430 440 450 460 470 480 QLLREKLRSH FGKRLDILGT AEYYSLDQMS YESIDFVIST IPIKKELPVP VLKVNTILGG 490 500 510 520 530 540 TDFTKIESIL SDEKEKANRY LKKELVFFQE DLRSKEEVIQ FLGQKVVECG FADEEIIDSI 550 560 570 580 590 600 FEREDMSPTC FGNLVAIPHP LVPQTKTTFW AVCTLKKPID WESQRVQFVC LLCVEKENKA 610 620 630 640 DLQSMYKLLG SILDDPAAMN QLIKCRSYQE LSDVFDQKML S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of a new beta-glucoside utilization system in Bacillus subtilis." Tobisch S., Glaser P., Krueger S., Hecker M. J. Bacteriol. 179:496-506(1997) [PubMed: 8990303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"Sequencing of a 65 kb region of the Bacillus subtilis genome containing the lic and cel loci, and creation of a 177 kb contig covering the gnt-sacXY region." Yoshida K., Shindo K., Sano H., Seki S., Fujimura M., Yanai N., Miwa Y., Fujita Y. Microbiology 142:3113-3123(1996) [PubMed: 8969509] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / BGSC1A1.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[4]	"Regulation of the lic operon of Bacillus subtilis and characterization of potential phosphorylation sites of the LicR regulator protein by site-directed mutagenesis." Tobisch S., Stuelke J., Hecker M. J. Bacteriol. 181:4995-5003(1999) [PubMed: 10438772] [Abstract] Cited for: MUTAGENESIS OF HIS-219; HIS-278; HIS-333; HIS-392 AND HIS-559.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z49992 Genomic DNA. Translation: CAA90284.1. D83026 Genomic DNA. Translation: BAA11742.1. AL009126 Genomic DNA. Translation: CAB15886.1.
PIR	H69651.
RefSeq	NP_391739.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	937383.
GenomeReviews	Gene locus BSU38600 in contig AL009126_GR.
KEGG	bsu:BSU38600.
NMPDR	fig|224308.1.peg.3865.
Organism-specific databases
SubtiList	BG11346. licR. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	P46321.
OMA	IHIAIAL.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU3856-MON.
BRENDA	2.7.1.69. 150.
Family and domain databases
InterPro	IPR013196. HTH_11. IPR007737. Mga. IPR011608. PRD. IPR002178. PTS_EIIA_2. IPR013011. PTS_EIIB_2. IPR001550. Transcrpt_antitermin_CS. [Graphical view]
Gene3D	G3DSA:1.10.1790.10. PRD. 2 hits. G3DSA:3.40.930.10. PTS_EIIA_2. 1 hit.
Pfam	PF08279. HTH_11. 1 hit. PF05043. Mga. 1 hit. PF00874. PRD. 2 hits. PF00359. PTS_EIIA_2. 1 hit. [Graphical view]
ProDom	PD001689. PTS_EIIA_2. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00654. PRD_1. False negative. PS51372. PRD_2. 2 hits. PS51094. PTS_EIIA_TYPE_2. 1 hit. PS51099. PTS_EIIB_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LICR_BACSU

Accession

Primary (citable) accession number: P46321

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 3, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)