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Protein

Probable 6-phospho-beta-glucosidase

Gene

licH

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Hydrolyzes phospho-beta-glucosides.Curated

Catalytic activityi

6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H2O = D-glucose + D-glucose 6-phosphate.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei96 – 961SubstrateBy similarity
Sitei112 – 1121Increases basicity of active site TyrBy similarity
Binding sitei150 – 1501SubstrateBy similarity
Metal bindingi172 – 1721ManganeseBy similarity
Metal bindingi202 – 2021ManganeseBy similarity
Active sitei256 – 2561Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi5 – 7369NADBy similarityAdd
BLAST

GO - Molecular functioni

  1. 6-phospho-beta-glucosidase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
  3. oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Source: InterPro

GO - Biological processi

  1. carbohydrate metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Manganese, Metal-binding, NAD

Enzyme and pathway databases

BioCyciBSUB:BSU38560-MONOMER.

Protein family/group databases

CAZyiGH4. Glycoside Hydrolase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable 6-phospho-beta-glucosidase (EC:3.2.1.86)
Gene namesi
Name:licH
Synonyms:celD, celF
Ordered Locus Names:BSU38560
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU38560. [Micado]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 442442Probable 6-phospho-beta-glucosidasePRO_0000169861Add
BLAST

Proteomic databases

PaxDbiP46320.

Expressioni

Inductioni

Induced by lichenan, lichenan hydrolysate and cellobiose. Subject to carbon catabolite repression.

Interactioni

Protein-protein interaction databases

STRINGi224308.BSU38560.

Structurei

3D structure databases

ProteinModelPortaliP46320.
SMRiP46320. Positions 5-439.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 4 family.Curated

Phylogenomic databases

eggNOGiCOG1486.
HOGENOMiHOG000239811.
InParanoidiP46320.
KOiK01222.
OMAiDIIRDME.
OrthoDBiEOG6CP3SG.
PhylomeDBiP46320.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR019802. GlycHydrolase_4_CS.
IPR001088. Glyco_hydro_4.
IPR022616. Glyco_hydro_4_C.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF02056. Glyco_hydro_4. 1 hit.
PF11975. Glyco_hydro_4C. 1 hit.
[Graphical view]
PRINTSiPR00732. GLHYDRLASE4.
SUPFAMiSSF56327. SSF56327. 1 hit.
PROSITEiPS01324. GLYCOSYL_HYDROL_F4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P46320-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKGLKIVTI GGGSSYTPEL VEGFIKRYDE LPVRELWLVD IPEGEEKLNI
60 70 80 90 100
VGTLAKRMVE KAGVPIDIHL TLDRRKALKD ADFVTTQFRV GLLQARAKDE
110 120 130 140 150
RIPLKYGVIG QETNGPGGLF KGLRTIPVIL EIAKDIEELC PNAWLVNFTN
160 170 180 190 200
PAGMVTEALL RYSNLKKVVG LCNVPIGIKM GVAKALDVDV DRVEVQFAGL
210 220 230 240 250
NHMVFGLDVF LDGVSVKEQV IEAMGDPKNA MTMKNISGAE WEPDFLKALN
260 270 280 290 300
VIPCGYHRYY FKTKEMLEHE LEASQTEGTR AEVVQKVEKE LFELYKDPNL
310 320 330 340 350
AIKPPQLEKR GGAYYSDAAC NLISSIYNDK HDIQPVNTIN NGAIASIPDD
360 370 380 390 400
SAVEVNCVMT KTGPKPIAVG DLPVSVRGLV QQIKSFERVA AEAAVTGDYQ
410 420 430 440
TALLAMTINP LVPSDTVAKQ ILDEMLEAHK AYLPQFFNKI EA
Length:442
Mass (Da):48,711
Last modified:November 1, 1995 - v1
Checksum:i91CB42C8BB93F567
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49992 Genomic DNA. Translation: CAA90288.1.
AL009126 Genomic DNA. Translation: CAB15882.1.
D83026 Genomic DNA. Translation: BAA11746.1.
PIRiS57762.
RefSeqiNP_391735.1. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB15882; CAB15882; BSU38560.
GeneIDi937373.
KEGGibsu:BSU38560.
PATRICi18979760. VBIBacSub10457_4042.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49992 Genomic DNA. Translation: CAA90288.1.
AL009126 Genomic DNA. Translation: CAB15882.1.
D83026 Genomic DNA. Translation: BAA11746.1.
PIRiS57762.
RefSeqiNP_391735.1. NC_000964.3.

3D structure databases

ProteinModelPortaliP46320.
SMRiP46320. Positions 5-439.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.BSU38560.

Protein family/group databases

CAZyiGH4. Glycoside Hydrolase Family 4.

Proteomic databases

PaxDbiP46320.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15882; CAB15882; BSU38560.
GeneIDi937373.
KEGGibsu:BSU38560.
PATRICi18979760. VBIBacSub10457_4042.

Organism-specific databases

GenoListiBSU38560. [Micado]

Phylogenomic databases

eggNOGiCOG1486.
HOGENOMiHOG000239811.
InParanoidiP46320.
KOiK01222.
OMAiDIIRDME.
OrthoDBiEOG6CP3SG.
PhylomeDBiP46320.

Enzyme and pathway databases

BioCyciBSUB:BSU38560-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR019802. GlycHydrolase_4_CS.
IPR001088. Glyco_hydro_4.
IPR022616. Glyco_hydro_4_C.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF02056. Glyco_hydro_4. 1 hit.
PF11975. Glyco_hydro_4C. 1 hit.
[Graphical view]
PRINTSiPR00732. GLHYDRLASE4.
SUPFAMiSSF56327. SSF56327. 1 hit.
PROSITEiPS01324. GLYCOSYL_HYDROL_F4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of a new beta-glucoside utilization system in Bacillus subtilis."
    Tobisch S., Glaser P., Krueger S., Hecker M.
    J. Bacteriol. 179:496-506(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  3. "Sequencing of a 65 kb region of the Bacillus subtilis genome containing the lic and cel loci, and creation of a 177 kb contig covering the gnt-sacXY region."
    Yoshida K., Shindo K., Sano H., Seki S., Fujimura M., Yanai N., Miwa Y., Fujita Y.
    Microbiology 142:3113-3123(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-305.
    Strain: 168 / BGSC1A1.

Entry informationi

Entry nameiLICH_BACSU
AccessioniPrimary (citable) accession number: P46320
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: January 7, 2015
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.