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Protein

Probable 6-phospho-beta-glucosidase

Gene

licH

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Hydrolyzes phospho-beta-glucosides.Curated

Catalytic activityi

6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H2O = D-glucose + D-glucose 6-phosphate.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei96SubstrateBy similarity1
Sitei112Increases basicity of active site TyrBy similarity1
Binding sitei150SubstrateBy similarity1
Metal bindingi172ManganeseBy similarity1
Metal bindingi202ManganeseBy similarity1
Active sitei256Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi5 – 73NADBy similarityAdd BLAST69

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Manganese, Metal-binding, NAD

Enzyme and pathway databases

BioCyciBSUB:BSU38560-MONOMER.

Protein family/group databases

CAZyiGH4. Glycoside Hydrolase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable 6-phospho-beta-glucosidase (EC:3.2.1.86)
Gene namesi
Name:licH
Synonyms:celD, celF
Ordered Locus Names:BSU38560
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001698611 – 442Probable 6-phospho-beta-glucosidaseAdd BLAST442

Proteomic databases

PaxDbiP46320.
PRIDEiP46320.

Expressioni

Inductioni

Induced by lichenan, lichenan hydrolysate and cellobiose. Subject to carbon catabolite repression.

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100020816.

Structurei

3D structure databases

ProteinModelPortaliP46320.
SMRiP46320.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 4 family.Curated

Phylogenomic databases

eggNOGiENOG4105F8D. Bacteria.
COG1486. LUCA.
HOGENOMiHOG000239811.
InParanoidiP46320.
KOiK01222.
OMAiLIPCGYH.
PhylomeDBiP46320.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR019802. GlycHydrolase_4_CS.
IPR001088. Glyco_hydro_4.
IPR022616. Glyco_hydro_4_C.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF02056. Glyco_hydro_4. 1 hit.
PF11975. Glyco_hydro_4C. 1 hit.
[Graphical view]
PRINTSiPR00732. GLHYDRLASE4.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
PROSITEiPS01324. GLYCOSYL_HYDROL_F4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P46320-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKGLKIVTI GGGSSYTPEL VEGFIKRYDE LPVRELWLVD IPEGEEKLNI
60 70 80 90 100
VGTLAKRMVE KAGVPIDIHL TLDRRKALKD ADFVTTQFRV GLLQARAKDE
110 120 130 140 150
RIPLKYGVIG QETNGPGGLF KGLRTIPVIL EIAKDIEELC PNAWLVNFTN
160 170 180 190 200
PAGMVTEALL RYSNLKKVVG LCNVPIGIKM GVAKALDVDV DRVEVQFAGL
210 220 230 240 250
NHMVFGLDVF LDGVSVKEQV IEAMGDPKNA MTMKNISGAE WEPDFLKALN
260 270 280 290 300
VIPCGYHRYY FKTKEMLEHE LEASQTEGTR AEVVQKVEKE LFELYKDPNL
310 320 330 340 350
AIKPPQLEKR GGAYYSDAAC NLISSIYNDK HDIQPVNTIN NGAIASIPDD
360 370 380 390 400
SAVEVNCVMT KTGPKPIAVG DLPVSVRGLV QQIKSFERVA AEAAVTGDYQ
410 420 430 440
TALLAMTINP LVPSDTVAKQ ILDEMLEAHK AYLPQFFNKI EA
Length:442
Mass (Da):48,711
Last modified:November 1, 1995 - v1
Checksum:i91CB42C8BB93F567
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49992 Genomic DNA. Translation: CAA90288.1.
AL009126 Genomic DNA. Translation: CAB15882.1.
D83026 Genomic DNA. Translation: BAA11746.1.
PIRiS57762.
RefSeqiNP_391735.1. NC_000964.3.
WP_003244285.1. NZ_JNCM01000034.1.

Genome annotation databases

EnsemblBacteriaiCAB15882; CAB15882; BSU38560.
GeneIDi937373.
KEGGibsu:BSU38560.
PATRICi18979760. VBIBacSub10457_4042.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49992 Genomic DNA. Translation: CAA90288.1.
AL009126 Genomic DNA. Translation: CAB15882.1.
D83026 Genomic DNA. Translation: BAA11746.1.
PIRiS57762.
RefSeqiNP_391735.1. NC_000964.3.
WP_003244285.1. NZ_JNCM01000034.1.

3D structure databases

ProteinModelPortaliP46320.
SMRiP46320.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100020816.

Protein family/group databases

CAZyiGH4. Glycoside Hydrolase Family 4.

Proteomic databases

PaxDbiP46320.
PRIDEiP46320.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15882; CAB15882; BSU38560.
GeneIDi937373.
KEGGibsu:BSU38560.
PATRICi18979760. VBIBacSub10457_4042.

Phylogenomic databases

eggNOGiENOG4105F8D. Bacteria.
COG1486. LUCA.
HOGENOMiHOG000239811.
InParanoidiP46320.
KOiK01222.
OMAiLIPCGYH.
PhylomeDBiP46320.

Enzyme and pathway databases

BioCyciBSUB:BSU38560-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR019802. GlycHydrolase_4_CS.
IPR001088. Glyco_hydro_4.
IPR022616. Glyco_hydro_4_C.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF02056. Glyco_hydro_4. 1 hit.
PF11975. Glyco_hydro_4C. 1 hit.
[Graphical view]
PRINTSiPR00732. GLHYDRLASE4.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
PROSITEiPS01324. GLYCOSYL_HYDROL_F4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLICH_BACSU
AccessioniPrimary (citable) accession number: P46320
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.