Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P46320

- LICH_BACSU

UniProt

P46320 - LICH_BACSU

Protein

Probable 6-phospho-beta-glucosidase

Gene

licH

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Hydrolyzes phospho-beta-glucosides.Curated

    Catalytic activityi

    6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H2O = D-glucose + D-glucose 6-phosphate.

    Cofactori

    NAD.By similarity
    Divalent metal ion.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei96 – 961SubstrateBy similarity
    Sitei112 – 1121Increases basicity of active site TyrBy similarity
    Binding sitei150 – 1501SubstrateBy similarity
    Metal bindingi172 – 1721ManganeseBy similarity
    Metal bindingi202 – 2021ManganeseBy similarity
    Active sitei256 – 2561Proton acceptorBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi5 – 7369NADBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. 6-phospho-beta-glucosidase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW
    3. oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Source: InterPro

    GO - Biological processi

    1. carbohydrate metabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism

    Keywords - Ligandi

    Manganese, Metal-binding, NAD

    Enzyme and pathway databases

    BioCyciBSUB:BSU38560-MONOMER.

    Protein family/group databases

    CAZyiGH4. Glycoside Hydrolase Family 4.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable 6-phospho-beta-glucosidase (EC:3.2.1.86)
    Gene namesi
    Name:licH
    Synonyms:celD, celF
    Ordered Locus Names:BSU38560
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU38560. [Micado]

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 442442Probable 6-phospho-beta-glucosidasePRO_0000169861Add
    BLAST

    Proteomic databases

    PaxDbiP46320.

    Expressioni

    Inductioni

    Induced by lichenan, lichenan hydrolysate and cellobiose. Subject to carbon catabolite repression.

    Interactioni

    Protein-protein interaction databases

    STRINGi224308.BSU38560.

    Structurei

    3D structure databases

    ProteinModelPortaliP46320.
    SMRiP46320. Positions 5-439.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 4 family.Curated

    Phylogenomic databases

    eggNOGiCOG1486.
    HOGENOMiHOG000239811.
    KOiK01222.
    OMAiNISGAEW.
    OrthoDBiEOG6CP3SG.
    PhylomeDBiP46320.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProiIPR019802. GlycHydrolase_4_CS.
    IPR001088. Glyco_hydro_4.
    IPR022616. Glyco_hydro_4_C.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF02056. Glyco_hydro_4. 1 hit.
    PF11975. Glyco_hydro_4C. 1 hit.
    [Graphical view]
    PRINTSiPR00732. GLHYDRLASE4.
    SUPFAMiSSF56327. SSF56327. 1 hit.
    PROSITEiPS01324. GLYCOSYL_HYDROL_F4. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P46320-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTKGLKIVTI GGGSSYTPEL VEGFIKRYDE LPVRELWLVD IPEGEEKLNI    50
    VGTLAKRMVE KAGVPIDIHL TLDRRKALKD ADFVTTQFRV GLLQARAKDE 100
    RIPLKYGVIG QETNGPGGLF KGLRTIPVIL EIAKDIEELC PNAWLVNFTN 150
    PAGMVTEALL RYSNLKKVVG LCNVPIGIKM GVAKALDVDV DRVEVQFAGL 200
    NHMVFGLDVF LDGVSVKEQV IEAMGDPKNA MTMKNISGAE WEPDFLKALN 250
    VIPCGYHRYY FKTKEMLEHE LEASQTEGTR AEVVQKVEKE LFELYKDPNL 300
    AIKPPQLEKR GGAYYSDAAC NLISSIYNDK HDIQPVNTIN NGAIASIPDD 350
    SAVEVNCVMT KTGPKPIAVG DLPVSVRGLV QQIKSFERVA AEAAVTGDYQ 400
    TALLAMTINP LVPSDTVAKQ ILDEMLEAHK AYLPQFFNKI EA 442
    Length:442
    Mass (Da):48,711
    Last modified:November 1, 1995 - v1
    Checksum:i91CB42C8BB93F567
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49992 Genomic DNA. Translation: CAA90288.1.
    AL009126 Genomic DNA. Translation: CAB15882.1.
    D83026 Genomic DNA. Translation: BAA11746.1.
    PIRiS57762.
    RefSeqiNP_391735.1. NC_000964.3.

    Genome annotation databases

    EnsemblBacteriaiCAB15882; CAB15882; BSU38560.
    GeneIDi937373.
    KEGGibsu:BSU38560.
    PATRICi18979760. VBIBacSub10457_4042.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49992 Genomic DNA. Translation: CAA90288.1 .
    AL009126 Genomic DNA. Translation: CAB15882.1 .
    D83026 Genomic DNA. Translation: BAA11746.1 .
    PIRi S57762.
    RefSeqi NP_391735.1. NC_000964.3.

    3D structure databases

    ProteinModelPortali P46320.
    SMRi P46320. Positions 5-439.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU38560.

    Protein family/group databases

    CAZyi GH4. Glycoside Hydrolase Family 4.

    Proteomic databases

    PaxDbi P46320.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB15882 ; CAB15882 ; BSU38560 .
    GeneIDi 937373.
    KEGGi bsu:BSU38560.
    PATRICi 18979760. VBIBacSub10457_4042.

    Organism-specific databases

    GenoListi BSU38560. [Micado ]

    Phylogenomic databases

    eggNOGi COG1486.
    HOGENOMi HOG000239811.
    KOi K01222.
    OMAi NISGAEW.
    OrthoDBi EOG6CP3SG.
    PhylomeDBi P46320.

    Enzyme and pathway databases

    BioCyci BSUB:BSU38560-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProi IPR019802. GlycHydrolase_4_CS.
    IPR001088. Glyco_hydro_4.
    IPR022616. Glyco_hydro_4_C.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF02056. Glyco_hydro_4. 1 hit.
    PF11975. Glyco_hydro_4C. 1 hit.
    [Graphical view ]
    PRINTSi PR00732. GLHYDRLASE4.
    SUPFAMi SSF56327. SSF56327. 1 hit.
    PROSITEi PS01324. GLYCOSYL_HYDROL_F4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and characterization of a new beta-glucoside utilization system in Bacillus subtilis."
      Tobisch S., Glaser P., Krueger S., Hecker M.
      J. Bacteriol. 179:496-506(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    3. "Sequencing of a 65 kb region of the Bacillus subtilis genome containing the lic and cel loci, and creation of a 177 kb contig covering the gnt-sacXY region."
      Yoshida K., Shindo K., Sano H., Seki S., Fujimura M., Yanai N., Miwa Y., Fujita Y.
      Microbiology 142:3113-3123(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-305.
      Strain: 168 / BGSC1A1.

    Entry informationi

    Entry nameiLICH_BACSU
    AccessioniPrimary (citable) accession number: P46320
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3