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P46320 (LICH_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable 6-phospho-beta-glucosidase

EC=3.2.1.86
Gene names
Name:licH
Synonyms:celD, celF
Ordered Locus Names:BSU38560
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length442 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Hydrolyzes phospho-beta-glucosides Probable.

Catalytic activity

6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H2O = D-glucose + D-glucose 6-phosphate.

Cofactor

NAD By similarity.

Divalent metal ion By similarity.

Induction

Induced by lichenan, lichenan hydrolysate and cellobiose. Subject to carbon catabolite repression.

Sequence similarities

Belongs to the glycosyl hydrolase 4 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 442442Probable 6-phospho-beta-glucosidase
PRO_0000169861

Regions

Nucleotide binding5 – 7369NAD By similarity

Sites

Active site2561Proton acceptor By similarity
Metal binding1721Manganese By similarity
Metal binding2021Manganese By similarity
Binding site961Substrate By similarity
Binding site1501Substrate By similarity
Site1121Increases basicity of active site Tyr By similarity

Sequences

Sequence LengthMass (Da)Tools
P46320 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 91CB42C8BB93F567

FASTA44248,711
        10         20         30         40         50         60 
MTKGLKIVTI GGGSSYTPEL VEGFIKRYDE LPVRELWLVD IPEGEEKLNI VGTLAKRMVE 

        70         80         90        100        110        120 
KAGVPIDIHL TLDRRKALKD ADFVTTQFRV GLLQARAKDE RIPLKYGVIG QETNGPGGLF 

       130        140        150        160        170        180 
KGLRTIPVIL EIAKDIEELC PNAWLVNFTN PAGMVTEALL RYSNLKKVVG LCNVPIGIKM 

       190        200        210        220        230        240 
GVAKALDVDV DRVEVQFAGL NHMVFGLDVF LDGVSVKEQV IEAMGDPKNA MTMKNISGAE 

       250        260        270        280        290        300 
WEPDFLKALN VIPCGYHRYY FKTKEMLEHE LEASQTEGTR AEVVQKVEKE LFELYKDPNL 

       310        320        330        340        350        360 
AIKPPQLEKR GGAYYSDAAC NLISSIYNDK HDIQPVNTIN NGAIASIPDD SAVEVNCVMT 

       370        380        390        400        410        420 
KTGPKPIAVG DLPVSVRGLV QQIKSFERVA AEAAVTGDYQ TALLAMTINP LVPSDTVAKQ 

       430        440 
ILDEMLEAHK AYLPQFFNKI EA 

« Hide

References

« Hide 'large scale' references
[1]"Identification and characterization of a new beta-glucoside utilization system in Bacillus subtilis."
Tobisch S., Glaser P., Krueger S., Hecker M.
J. Bacteriol. 179:496-506(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"Sequencing of a 65 kb region of the Bacillus subtilis genome containing the lic and cel loci, and creation of a 177 kb contig covering the gnt-sacXY region."
Yoshida K., Shindo K., Sano H., Seki S., Fujimura M., Yanai N., Miwa Y., Fujita Y.
Microbiology 142:3113-3123(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-305.
Strain: 168 / BGSC1A1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49992 Genomic DNA. Translation: CAA90288.1.
AL009126 Genomic DNA. Translation: CAB15882.1.
D83026 Genomic DNA. Translation: BAA11746.1.
PIRS57762.
RefSeqNP_391735.1. NC_000964.3.

3D structure databases

ProteinModelPortalP46320.
SMRP46320. Positions 5-439.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224308.BSU38560.

Protein family/group databases

CAZyGH4. Glycoside Hydrolase Family 4.

Proteomic databases

PaxDbP46320.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB15882; CAB15882; BSU38560.
GeneID937373.
KEGGbsu:BSU38560.
PATRIC18979760. VBIBacSub10457_4042.

Organism-specific databases

GenoListBSU38560. [Micado]

Phylogenomic databases

eggNOGCOG1486.
HOGENOMHOG000239811.
KOK01222.
OMANISGAEW.
OrthoDBEOG6CP3SG.
PhylomeDBP46320.

Enzyme and pathway databases

BioCycBSUB:BSU38560-MONOMER.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProIPR019802. GlycHydrolase_4_CS.
IPR001088. Glyco_hydro_4.
IPR022616. Glyco_hydro_4_C.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamPF02056. Glyco_hydro_4. 1 hit.
PF11975. Glyco_hydro_4C. 1 hit.
[Graphical view]
PRINTSPR00732. GLHYDRLASE4.
SUPFAMSSF56327. SSF56327. 1 hit.
PROSITEPS01324. GLYCOSYL_HYDROL_F4. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLICH_BACSU
AccessionPrimary (citable) accession number: P46320
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 9, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList