Reviewed, UniProtKB/Swiss-Prot P46320 (LICH_BACSU)

Names and origin

Protein names

Recommended name:
    Probable 6-phospho-beta-glucosidase
    EC=3.2.1.86

Gene names

Name:	licH
Synonyms:	celD, celF
Ordered Locus Names:	BSU38560

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	442 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Hydrolyzes phospho-beta-glucosides Probable.
Catalytic activity	6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H2O = D-glucose + D-glucose 6-phosphate.
Cofactor	NAD By similarity. Divalent metal ion By similarity.
Induction	Induced by lichenan, lichenan hydrolysate and cellobiose. Subject to carbon catabolite repression.
Sequence similarities	Belongs to the glycosyl hydrolase 4 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Ligand	Manganese Metal-binding NAD
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	6-phospho-beta-glucosidase activity Inferred from electronic annotation. Source: EC manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 442	442	Probable 6-phospho-beta-glucosidase		PRO_0000169861
Regions
	Nucleotide binding	5 – 73	69	NAD By similarity
Sites
	Active site	256	1	Proton acceptor By similarity
	Metal binding	172	1	Manganese By similarity
	Metal binding	202	1	Manganese By similarity
	Binding site	96	1	Substrate By similarity
	Binding site	150	1	Substrate By similarity
	Site	112	1	Increases basicity of active site Tyr By similarity

Sequences

	Sequence		Length	Mass (Da)
	P46320-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 91CB42C8BB93F567	FASTA	442	48,711
	10 20 30 40 50 60 MTKGLKIVTI GGGSSYTPEL VEGFIKRYDE LPVRELWLVD IPEGEEKLNI VGTLAKRMVE 70 80 90 100 110 120 KAGVPIDIHL TLDRRKALKD ADFVTTQFRV GLLQARAKDE RIPLKYGVIG QETNGPGGLF 130 140 150 160 170 180 KGLRTIPVIL EIAKDIEELC PNAWLVNFTN PAGMVTEALL RYSNLKKVVG LCNVPIGIKM 190 200 210 220 230 240 GVAKALDVDV DRVEVQFAGL NHMVFGLDVF LDGVSVKEQV IEAMGDPKNA MTMKNISGAE 250 260 270 280 290 300 WEPDFLKALN VIPCGYHRYY FKTKEMLEHE LEASQTEGTR AEVVQKVEKE LFELYKDPNL 310 320 330 340 350 360 AIKPPQLEKR GGAYYSDAAC NLISSIYNDK HDIQPVNTIN NGAIASIPDD SAVEVNCVMT 370 380 390 400 410 420 KTGPKPIAVG DLPVSVRGLV QQIKSFERVA AEAAVTGDYQ TALLAMTINP LVPSDTVAKQ 430 440 ILDEMLEAHK AYLPQFFNKI EA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of a new beta-glucoside utilization system in Bacillus subtilis." Tobisch S., Glaser P., Krueger S., Hecker M. J. Bacteriol. 179:496-506(1997) [PubMed: 8990303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"Sequencing of a 65 kb region of the Bacillus subtilis genome containing the lic and cel loci, and creation of a 177 kb contig covering the gnt-sacXY region." Yoshida K., Shindo K., Sano H., Seki S., Fujimura M., Yanai N., Miwa Y., Fujita Y. Microbiology 142:3113-3123(1996) [PubMed: 8969509] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-305. Strain: 168 / BGSC1A1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z49992 Genomic DNA. Translation: CAA90288.1. AL009126 Genomic DNA. Translation: CAB15882.1. D83026 Genomic DNA. Translation: BAA11746.1.
PIR	S57762.
RefSeq	NP_391735.1.
3D structure databases
SMR	P46320. Positions 5-439.
ModBase	Search...
Protein family/group databases
CAZy	GH4. Glycoside Hydrolase Family 4.
Genome annotation databases
GeneID	937373.
GenomeReviews	Gene locus BSU38560 in contig AL009126_GR.
KEGG	bsu:BSU38560.
NMPDR	fig|224308.1.peg.3861.
Organism-specific databases
SubtiList	BG11350. licH. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	P46320.
OMA	P46320. AKLDIIF.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU3852-MON.
BRENDA	3.2.1.86. 150.
Family and domain databases
InterPro	IPR019802. GlycHydrolase_4_CS. IPR001088. Glyco_hydro_4. IPR015955. Lactate_DH/Glyco_Ohase_4_C. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit.
Pfam	PF02056. Glyco_hydro_4. 1 hit. [Graphical view]
PRINTS	PR00732. GLHYDRLASE4.
ProDom	PD006892. Glyco_hydro_4. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS01324. GLYCOSYL_HYDROL_F4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LICH_BACSU

Accession

Primary (citable) accession number: P46320

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)