P46318 (PTJB_BACSU) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 92.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Lichenan-specific phosphotransferase enzyme IIB component EC=2.7.1.69 Alternative name(s): EIIB-Lic PTS system lichenan-specific EIIB component | ||||||
| Gene names |
| ||||||
| Organism | Bacillus subtilis | ||||||
| Taxonomic identifier | 1423 [NCBI] | ||||||
| Taxonomic lineage | Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus |
Protein attributes
| Sequence length | 102 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in lichenan transport. |
| Catalytic activity | Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate. |
| Subcellular location | Cytoplasm Potential. |
| Induction | Induced by lichenan, lichenan hydrolysate and cellobiose. Subject to carbon catabolite repression. |
| Domain | The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain. |
| Sequence similarities | Contains 1 PTS EIIB type-3 domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Phosphotransferase system Sugar transport Transport |
| Cellular component | Cytoplasm |
| Molecular function | Kinase Transferase |
| PTM | Phosphoprotein |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activityInferred from electronic annotation. Source: EC sugar bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 102 | 102 | Lichenan-specific phosphotransferase enzyme IIB component | PRO_0000186487 | |||||
Regions | |||||||||
| Domain | 1 – 102 | 102 | PTS EIIB type-3 | ||||||
Sites | |||||||||
| Active site | 7 | 1 | Phosphocysteine intermediate By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 37 | 1 | Phosphoserine Ref.4 | ||||||
Sequences
References
| « Hide 'large scale' references | |
| [1] | "Identification and characterization of a new beta-glucoside utilization system in Bacillus subtilis." Tobisch S., Glaser P., Krueger S., Hecker M. J. Bacteriol. 179:496-506(1997) [PubMed: 8990303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168. |
| [2] | "Sequencing of a 65 kb region of the Bacillus subtilis genome containing the lic and cel loci, and creation of a 177 kb contig covering the gnt-sacXY region." Yoshida K., Shindo K., Sano H., Seki S., Fujimura M., Yanai N., Miwa Y., Fujita Y. Microbiology 142:3113-3123(1996) [PubMed: 8969509] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / BGSC1A1. |
| [3] | "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. Danchin A.Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168. |
| [4] | "The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis." Macek B., Mijakovic I., Olsen J.V., Gnad F., Kumar C., Jensen P.R., Mann M. Mol. Cell. Proteomics 6:697-707(2007) [PubMed: 17218307] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-37, MASS SPECTROMETRY. Strain: 168. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | Z49992 Genomic DNA. Translation: CAA90285.1. D83026 Genomic DNA. Translation: BAA11743.1. AL009126 Genomic DNA. Translation: CAB15885.1. |
| PIR | E69651. |
| RefSeq | NP_391738.1. NC_000964.3. |
3D structure databases | |
| ProteinModelPortal | P46318. |
| SMR | P46318. Positions 1-102. |
| ModBase | Search... |
Protein family/group databases | |
| TCDB | 4.A.3.2.2. PTS lactose-N,N'-diacetylchitobiose-beta,-glucoside (Lac) family. |
PTM databases | |
| PhosSite | P46318. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblBacteria | EBBACT00000000040; EBBACP00000000040; EBBACG00000000040. |
| GeneID | 937379. |
| GenomeReviews | Gene locus BSU38590 in contig AL009126_GR. |
| KEGG | bsu:BSU38590. |
| NMPDR | fig|224308.1.peg.3864. |
| PATRIC | 18979766. VBIBacSub10457_4045. |
Organism-specific databases | |
| GenoList | BSU38590. [Micado] |
Phylogenomic databases | |
| GeneTree | EBGT00050000001261. |
| HOGENOM | HBG697211. |
| OMA | KTIMLAC. |
| PhylomeDB | P46318. |
| ProtClustDB | CLSK888115. |
Enzyme and pathway databases | |
| BioCyc | BSUB:BSU38590-MONOMER. |
Family and domain databases | |
| InterPro | IPR014350. PTrfase_system_EIIB_3_subgr. IPR003501. PTS_EIIB_2/3. IPR013012. PTS_EIIB_3. [Graphical view] |
| KO | K02760. |
| Pfam | PF02302. PTS_IIB. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR00853. Pts-lac. 1 hit. |
| PROSITE | PS51100. PTS_EIIB_TYPE_3. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | PTJB_BACSU | ||||||||
| Accession | Primary (citable) accession number: P46318 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| Bacillus subtilis Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList |
| SIMILARITY comments Index of protein domains and families |

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