P46286 (RL81_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
60S ribosomal protein L8-1

Alternative name(s):
60S ribosomal protein L2
Protein EMBRYO DEFECTIVE 2296

Gene names

Name:	RPL8A
Synonyms:	EMB2296, RPL2
Ordered Locus Names:	At2g18020
ORF Names:	T27K22.11

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Cytoplasm.
Post-translational modification	Ubiquitinated. Ref.6
Sequence similarities	Belongs to the ribosomal protein L2P family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: InterPro
Cellular component	chloroplast Inferred from direct assay. Source: TAIR cytosolic large ribosomal subunit Inferred from direct assay. Source: TAIR nucleolus Inferred from direct assay. Source: TAIR plasma membrane Inferred from direct assay. Source: TAIR vacuolar membrane Inferred from direct assay. Source: TAIR
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: TAIR structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 258	258	60S ribosomal protein L8-1		PRO_0000129733
Experimental info
	Sequence conflict	81 – 87	7	GMYTGQF → VCTPVSS in CAA60445. Ref.1
	Sequence conflict	139	1	H → E in CAA60445. Ref.1
	Sequence conflict	162	1	C → S in CAA60445. Ref.1
	Sequence conflict	222	1	A → S in CAA60445. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P46286 [UniParc]. Last modified November 2, 2001. Version 2. Checksum: C88C5942DF9AC4B2	FASTA	258	27,859
	10 20 30 40 50 60 MGRVIRAQRK GAGSVFKSHT HHRKGPAKFR SLDFGERNGY LKGVVTEIIH DPGRGAPLAR 70 80 90 100 110 120 VTFRHPFRFK KQKELFVAAE GMYTGQFLYC GKKATLVVGN VLPLRSIPEG AVVCNVEHHV 130 140 150 160 170 180 GDRGVLARAS GDYAIVIAHN PDSDTTRIKL PSGSKKIVPS GCRAMIGQVA GGGRTEKPML 190 200 210 220 230 240 KAGNAYHKYR VKRNSWPKVR GVAMNPVEHP HGGGNHQHIG HASTVRRDAP PGQKVGLIAA 250 RRTGRLRGQA AASAAKAD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Schuster W. Submitted (APR-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. C24.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"The organization of cytoplasmic ribosomal protein genes in the Arabidopsis genome." Barakat A., Szick-Miranda K., Chang I.-F., Guyot R., Blanc G., Cooke R., Delseny M., Bailey-Serres J. Plant Physiol. 127:398-415(2001) [PubMed: 11598216] [Abstract] Cited for: GENE FAMILY ORGANIZATION, NOMENCLATURE.
[6]	"Tandem affinity purification and mass spectrometric analysis of ubiquitylated proteins in Arabidopsis." Saracco S.A., Hansson M., Scalf M., Walker J.M., Smith L.M., Vierstra R.D. Plant J. 59:344-358(2009) [PubMed: 19292762] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X86765 mRNA. Translation: CAA60445.1. AC006201 Genomic DNA. Translation: AAD20124.1. CP002685 Genomic DNA. Translation: AEC06716.1. AY128314 mRNA. Translation: AAM91517.1. BT001177 mRNA. Translation: AAN65064.1.
IPI	IPI00517953.
PIR	C84559.
RefSeq	NP_179393.1. NM_127358.5.
UniGene	At.24098. At.71470. At.74928.
3D structure databases
ProteinModelPortal	P46286.
SMR	P46286. Positions 1-255.
ModBase	Search...
Protein-protein interaction databases
IntAct	P46286. 1 interaction.
STRING	P46286.
Proteomic databases
PRIDE	P46286.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G18020.1; AT2G18020.1; AT2G18020.
GeneID	816314.
GenomeReviews	Gene locus AT2G18020 in contig CT485783_GR.
KEGG	ath:AT2G18020.
NMPDR	fig|3702.1.peg.8797.
Organism-specific databases
GeneFarm	405. 43.
TAIR	At2g18020.
Phylogenomic databases
eggNOG	KOG2309.
HOGENOM	HBG748739.
InParanoid	P46286.
OMA	PFVKAGK.
PhylomeDB	P46286.
ProtClustDB	CLSN2683927.
Gene expression databases
ArrayExpress	P46286.
Genevestigator	P46286.
GermOnline	AT2G18020. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR022666. Rbsml_prot_L2_RNA-bd_dom. IPR002171. Ribosomal_L2. IPR014726. Ribosomal_L2_3. IPR023672. Ribosomal_L2_arc. IPR022669. Ribosomal_L2_C. IPR022671. Ribosomal_L2_CS. IPR014722. Transl_SH3-like_sub. IPR008991. Translation_prot_SH3-like. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:2.30.30.30. Ribosomal_L2. 1 hit. G3DSA:4.10.950.10. Ribosomal_L2. 1 hit.
KO	K02938.
PANTHER	PTHR13691. Ribosomal_L2. 1 hit.
Pfam	PF00181. Ribosomal_L2. 1 hit. PF03947. Ribosomal_L2_C. 1 hit. [Graphical view]
PIRSF	PIRSF002158. Ribosomal_L2. 1 hit.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF50104. Transl_SH3_like. 1 hit.
PROSITE	PS00467. RIBOSOMAL_L2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RL81_ARATH

Accession

Primary (citable) accession number: P46286
Secondary accession number(s): Q9SL44

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 2, 2001
Last modified:	November 16, 2011
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program