P46273 (PGK_CANAL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoglycerate kinase
EC=2.7.2.3

Gene names

Name:	PGK1
ORF Names:	CaO19.11135, CaO19.3651

Organism

Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) [Reference proteome]

Taxonomic identifier

237561 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida › Candida albicans

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.
Subunit structure	Monomer.
Subcellular location	Cytoplasm. Secreted › cell wall.
Sequence similarities	Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation Glycolysis
Cellular component	Cell wall Cytoplasm Secreted
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycolysis Non-traceable author statement PubMed 7997178. Source: CGD induction by symbiont of host defense response Inferred from direct assay PubMed 11681208. Source: CGD
Cellular_component	cytoplasm Inferred from direct assay Ref.1. Source: CGD external side of plasma membrane Inferred from direct assay Ref.1. Source: CGD extracellular region Inferred from electronic annotation. Source: UniProtKB-KW hyphal cell wall Inferred from direct assay PubMed 12782322PubMed 1383159. Source: CGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoglycerate kinase activity Inferred from sequence or structural similarity Ref.1. Source: CGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 417	417	Phosphoglycerate kinase		PRO_0000145876
Regions
	Nucleotide binding	372 – 375	4	ATP By similarity
	Region	24 – 26	3	Substrate binding By similarity
	Region	63 – 66	4	Substrate binding By similarity
Sites
	Binding site	39	1	Substrate By similarity
	Binding site	122	1	Substrate By similarity
	Binding site	170	1	Substrate By similarity
	Binding site	219	1	ATP By similarity
	Binding site	312	1	ATP; via carbonyl oxygen By similarity
	Binding site	343	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P46273 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 6F147F640F5EC784	FASTA	417	45,180
	10 20 30 40 50 60 MSLSNKLSVK DLDVAGKRVF IRVDFNVPLD GKTITNNQRI VAALPTIKYV EEHKPKYIVL 70 80 90 100 110 120 ASHLGRPNGE RNDKYSLAPV ATELEKLLGQ KVTFLNDCVG PEVTKAVENA KDGEIFLLEN 130 140 150 160 170 180 LRYHIEEEGS SKDKDGKKVK ADPEAVKKFR QELTSLADVY INDAFGTAHR AHSSMVGLEV 190 200 210 220 230 240 PQRAAGFLMS KELEYFAKAL ENPERPFLAI LGGAKVSDKI QLIDNLLDKV DMLIVGGGMA 250 260 270 280 290 300 FTFKKILNKM PIGDSLFDEA GAKNVEHLVE KAKKNNVELI LPVDFVTADK FDKDAKTSSA 310 320 330 340 350 360 TDAEGIPDNW MGLDCGPKSV ELFQQAVAKA KTIVWNGPPG VFEFEKFANG TKSLLDAAVK 370 380 390 400 410 SAENGNIVII GGGDTATVAK KYGVVEKLSH VSTGGGASLE LLEGKDLPGV VALSNKN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"3-phosphoglycerate kinase: a glycolytic enzyme protein present in the cell wall of Candida albicans." Alloush H.M., Lopez-Ribot J.L., Masten B.J., Chaffin W.L. Microbiology 143:321-330(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 3153A.
[2]	"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314 / ATCC MYA-2876.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U25180 Genomic DNA. Translation: AAA66523.1. AACQ01000203 Genomic DNA. Translation: EAK92092.1. AACQ01000201 Genomic DNA. Translation: EAK92141.1.
RefSeq	XP_711323.1. XM_706231.1. XP_711371.1. XM_706279.1.
3D structure databases
ProteinModelPortal	P46273.
SMR	P46273. Positions 2-416.
ModBase	Search...
Protein-protein interaction databases
STRING	5476.CAL0000415.
Protein family/group databases
Allergome	5991. Cand a PGK.
2D gel databases
COMPLUYEAST-2DPAGE	P46273.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3647038. 3647081.
KEGG	cal:CaO19.11135. cal:CaO19.3651.
Organism-specific databases
CGD	CAL0000415. PGK1.
Phylogenomic databases
eggNOG	COG0126.
KO	K00927.
Enzyme and pathway databases
BRENDA	2.7.2.3. 1096.
UniPathway	UPA00109; UER00185.
Family and domain databases
Gene3D	3.40.50.1260. 1 hit. 3.40.50.1270. 1 hit.
InterPro	IPR001576. Phosphoglycerate_kinase. IPR015901. Phosphoglycerate_kinase_C. IPR015911. Phosphoglycerate_kinase_CS. IPR015824. Phosphoglycerate_kinase_N. [Graphical view]
PANTHER	PTHR11406. PTHR11406. 1 hit.
Pfam	PF00162. PGK. 1 hit. [Graphical view]
PIRSF	PIRSF000724. Pgk. 1 hit.
PRINTS	PR00477. PHGLYCKINASE.
SUPFAM	SSF53748. PGK. 1 hit.
PROSITE	PS00111. PGLYCERATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGK_CANAL

Accession

Primary (citable) accession number: P46273
Secondary accession number(s): Q59NN7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program