Reviewed, UniProtKB/Swiss-Prot P46257 (ALF2_PEA)

Names and origin

Protein names	Recommended name:     Fructose-bisphosphate aldolase, cytoplasmic isozyme 2     EC=4.1.2.13
Organism	Pisum sativum (Garden pea)
Taxonomic identifier	3888 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Fabeae › Pisum

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class I fructose-bisphosphate aldolase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	fructose-bisphosphate aldolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 359	359	Fructose-bisphosphate aldolase, cytoplasmic isozyme 2		PRO_0000216924
Sites
	Active site	184	1	Proton acceptor By similarity
	Active site	226	1	Schiff-base intermediate with dihydroxyacetone-P By similarity
	Binding site	52	1	Substrate By similarity
	Binding site	143	1	Substrate By similarity
	Site	359	1	Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P46257-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: C0CAB16E9CC1B9EF	FASTA	359	38,491
	10 20 30 40 50 60 MSHFKSKYHD ELIANAAYIG TPGKGILAAD ESTGTIGKRL SSINVENVES NRQALRELLF 70 80 90 100 110 120 TASWLFLQYL SGVILFEETL YQKTAAGKPF VDVLNEAGVL PGIKVDKGTV ELAGTDGETT 130 140 150 160 170 180 TQGLDGLGAR CRKYYEAGAR FAKWRAVLKI GANEPSEHSI HENAYGLARY AVICQENGLV 190 200 210 220 230 240 PIVEPEILVD GSHDILKCAA ITERVLAATY KALSDHHVIL EGTLLKPNMV TPGSDAPKVA 250 260 270 280 290 300 PEVIAEHTVR ALQRTVPAAV PAVVFLSGGQ SEEEASVNLN AINQIKGKKP WTLSFSFGRA 310 320 330 340 350 LQQSTLKAWG GKTENVKAAQ DALLTRAKAN SEATLGTYKG ASNLGAGASE SLHVKDYKY

References

[1]	Pelzer-Reith B., Schnarrenberger C. Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Leaf.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X89829 Genomic DNA. Translation: CAA61947.1.
PIR	S58167.
3D structure databases
SMR	P46257. Positions 11-359.
ModBase	Search...
Enzyme and pathway databases
BRENDA	4.1.2.13. 287.
Family and domain databases
InterPro	IPR000741. Aldolase_I. IPR013785. Aldolase_TIM. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR11627. Aldolase_I. 1 hit.
Pfam	PF00274. Glycolytic. 1 hit. [Graphical view]
PROSITE	PS00158. ALDOLASE_CLASS_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALF2_PEA

Accession

Primary (citable) accession number: P46257

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	February 9, 2010
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)