P46256 (ALF1_PEA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Fructose-bisphosphate aldolase, cytoplasmic isozyme 1 EC=4.1.2.13
Organism	Pisum sativum (Garden pea)
Taxonomic identifier	3888 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Fabeae › Pisum

Protein attributes

Sequence length	357 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class I fructose-bisphosphate aldolase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	fructose-bisphosphate aldolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 357	357	Fructose-bisphosphate aldolase, cytoplasmic isozyme 1		PRO_0000216923
Sites
	Active site	183	1	Proton acceptor By similarity
	Active site	225	1	Schiff-base intermediate with dihydroxyacetone-P By similarity
	Binding site	52	1	Substrate By similarity
	Binding site	142	1	Substrate By similarity
	Site	357	1	Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P46256 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: BB30E70BA0401D61	FASTA	357	38,446
	10 20 30 40 50 60 MSAFVGKYAD ELIKNAKYIA TPGKGILAAD ESTGTIGKRL ASINVENIEA NRQALRELLF 70 80 90 100 110 120 TSPNALQYLS GVILFEETLY QKSSEGKPFV EILQENNVIP GIKVDKGVVE LAGTDGETTT 130 140 150 160 170 180 QGFDSLGARC QQYYKAGARF AKWRAVLKIG PNEPSELSIQ QNAQGLARYA IICQENGLVL 190 200 210 220 230 240 FVEPEILTDG SHDIAKCAAV TETVLAACYK ALNDQHVLLE GTLLKPNMVT PGSDSPKVSP 250 260 270 280 290 300 EVIGEYTVNA LRRTVPAAVP GIVFLSGGQS EEQATLNLNA MNKFDVVKPW TLSFSFGRAL 310 320 330 340 350 QQSTLKTWSG KKENVGKAQD VFLARCKANS EATLGKYGGG SGTGLASESL HVKDYKY

References

[1]	Pelzer-Reith B., Schnarrenberger C. Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Leaf.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X89828 mRNA. Translation: CAA61946.1.
PIR	S58168.
3D structure databases
ProteinModelPortal	P46256.
SMR	P46256. Positions 10-337.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000741. Aldolase_I. IPR013785. Aldolase_TIM. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR11627. Aldolase_I. 1 hit.
Pfam	PF00274. Glycolytic. 1 hit. [Graphical view]
PROSITE	PS00158. ALDOLASE_CLASS_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALF1_PEA

Accession

Primary (citable) accession number: P46256

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 31, 2011
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program