P46245 (AROH_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive
EC=2.5.1.54

Alternative name(s):
3-deoxy-D-arabino-heptulosonate 7-phosphate synthase
DAHP synthase
Phospho-2-keto-3-deoxyheptonate aldolase

Gene names

Name:	aroH
Ordered Locus Names:	BUsg_116

Organism

Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]

Taxonomic identifier

198804 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	348 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP).
Catalytic activity	Phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate.
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 1/7.
Sequence similarities	Belongs to the class-I DAHP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-deoxy-7-phosphoheptulonate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 348	348	Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive		PRO_0000140838
Experimental info
	Sequence conflict	137 – 142	6	MPAATE → CLQQQS in AAC43604. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P46245 [UniParc]. Last modified August 30, 2002. Version 2. Checksum: BD601FB68AFFD500	FASTA	348	39,148
	10 20 30 40 50 60 MKKTDELRTI RIDPLITPSE LAKKYAITSE IMDNVIITRQ NIARIMTGED LRLLVVIGPC 70 80 90 100 110 120 SVHDPIAAVE YAHRLYELRK KYQDRLEIIM RTYFEKPRTV VGWKGLISDP DLNGSFRVNH 130 140 150 160 170 180 GLAVARKLLL DINELGMPAA TEFLDMVIGQ FIADLISWGA IGARTTESQI HREMASALSC 190 200 210 220 230 240 PVGFKNGTDG NIRIAIDAIR AAQARHLFFA PNKDGQMTIN HTSGNPYGHI IMRGGRTPNY 250 260 270 280 290 300 HAHDINSAIE HLREFNLLEH LMIDFSHGNC LKEHIRQKDV AKSVSKQISQ GSKTIFGVMI 310 320 330 340 ESFLEEGFQT VKENQPLIYG KSITDACLNW KDSVLIIKQL ADAVDTRF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Aromatic amino acid biosynthesis in Buchnera aphidicola (endosymbiont of aphids): cloning and sequencing of a DNA fragment containing aroH-thrS-infC-rpmI-rplT." Kolibachuk D., Rouhbakhsh D., Baumann P. Curr. Microbiol. 30:313-316(1995) [PubMed: 7766160] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sg.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U11066 Genomic DNA. Translation: AAC43604.1. AE013218 Genomic DNA. Translation: AAM67685.1.
PIR	I40070.
RefSeq	NP_660474.1. NC_004061.1.
3D structure databases
ProteinModelPortal	P46245.
SMR	P46245. Positions 6-347.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000000265; EBBUCP00000000265; EBBUCG00000000265.
GeneID	1005933.
GenomeReviews	Gene locus BUsg_116 in contig AE013218_GR.
KEGG	bas:BUsg116.
PATRIC	21247013. VBIBucAph100086_0120.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000008184.
HOGENOM	HBG670536.
OMA	HRELASH.
ProtClustDB	PRK09261.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG116-MONOMER.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR006218. DAHP1/KDSA. IPR006219. DHAP_synth_1. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01626.
PANTHER	PTHR21225. AroFGH. 1 hit.
Pfam	PF00793. DAHP_synth_1. 1 hit. [Graphical view]
PIRSF	PIRSF001361. DAHP_synthase. 1 hit.
TIGRFAMs	TIGR00034. AroFGH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AROH_BUCAP

Accession

Primary (citable) accession number: P46245

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	August 30, 2002
Last modified:	January 25, 2012
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program