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Reviewed, UniProtKB/Swiss-Prot P46240 (AROE_BUCAP)

Last modified November 25, 2008. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Shikimate dehydrogenase
    EC=1.1.1.25
Gene names
Name: aroE
Ordered Locus Names: BUsg_474
OrganismBuchnera aphidicola subsp. Schizaphis graminum [Complete proteome] [HAMAP]
Taxonomic identifier98794 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

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Protein attributes

Sequence length273 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Catalytic activity

Shikimate + NADP(+) = 3-dehydroshikimate + NADPH.

Pathway

Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and PEP: step 4/7.

Sequence similarities

Belongs to the shikimate dehydrogenase family.

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Ontologies

Keywords

   Biological processAmino-acid biosynthesis
Aromatic amino acid biosynthesis
   LigandNADP
   Molecular functionOxidoreductase
   Technical termComplete proteome

Gene Ontology (GO)

   Biological processaromatic amino acid family biosynthetic process

Inferred from electronic annotation. Source: HAMAP

oxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: InterPro

   Molecular functionNADP binding

Inferred from electronic annotation. Source: InterPro

shikimate 5-dehydrogenase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 273273Shikimate dehydrogenase
PRO_0000135996

Regions

Nucleotide binding131 – 1355NADP By similarity

Sites

Active site701Proton acceptor Potential

Experimental info

Sequence conflict203 – 2042LP → T Ref.1
Sequence conflict2061S → I Ref.1
Sequence conflict2121T → R in AAD09433. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
P46240-1 [UniParc].

Last modified August 30, 2002. Version 2.
Checksum: 50507AF854774AF6

FASTA27330,885
        10         20         30         40         50         60 
MCKLEKFNYA LFGNPIDHSQ SPKIHNFFAT QTGILHIYKA INIPLDQFSS VVSDFFKKNI 

        70         80         90        100        110        120 
KGANVTAPFK KEAYFFSDKL TERAKIAQSV NTLKKISDKC ILGDNTDGIG LLSDLVRLNF 

       130        140        150        160        170        180 
IKKNFSILIL GAGGAVKGVL LPLLSLGCSV YILNRTILNA KILVKQFNKY GKIFVFDRQN 

       190        200        210        220        230        240 
FKQQNFDLVI NAMSRNTEKK NFLPLSLITS KTFFYDMNYS TRNTPFINWC SKAGGSFISN 

       250        260        270 
GIGMLVFQAA YSFLEWHNVL PEINYIINIL NIK

« Hide

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References

« Hide 'large scale' references
[1]"Characterization of a putative 23S-5S rRNA operon of Buchnera aphidicola (endosymbiont of aphids) unlinked to the 16S rRNA-encoding gene."
Rouhbakhsh D., Baumann P.
Gene 155:107-112(1995) [PubMed: 7535281] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"50 million years of genomic stasis in endosymbiotic bacteria."
Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.
Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases

U09230 Genomic DNA. Translation: AAD09433.1.
AE013218 Genomic DNA. Translation: AAM68017.1.
RefSeqNP_660806.1.

3D structure databases

HSSPHSSP built from PDB template 1NYT based on UniProtKB P15770.
ModBaseSearch...

Genome annotation databases

GeneID1005693.
GenomeReviewsGene locus BUsg_474 in contig AE013218_GR.
KEGGbas:BUsg474.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMP46240.

Enzyme and pathway databases

BioCycBAPH198804:BUSG474-MON.

Family and domain databases

HAMAPMF_00222.
[Tree]
InterProIPR011342. Quinate/shikimate_5-DHase.
IPR013708. Shikimate_DHase-bd_N.
IPR006151. Shikm_DHase/Glu-tRNA_Rdtase.
[Graphical view]
PfamPF01488. Shikimate_DH. 1 hit.
PF08501. Shikimate_dh_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR00507. aroE. 1 hit.
ProtoNetSearch...

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Entry information

Entry nameAROE_BUCAP
AccessionPrimary (citable) accession number: P46240
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: August 30, 2002
Last modified: November 25, 2008
This is version 61 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Buchnera aphidicola (subsp. Schizaphis graminum)

Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents