Reviewed, UniProtKB/Swiss-Prot P46240 (AROE_BUCAP)

Names and origin

Protein names

Recommended name:
    Shikimate dehydrogenase
    EC=1.1.1.25

Gene names

Name:	aroE
Ordered Locus Names:	BUsg_474

Organism

Buchnera aphidicola subsp. Schizaphis graminum [Complete proteome] [HAMAP]

Taxonomic identifier

98794 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Shikimate + NADP+ = 3-dehydroshikimate + NADPH. HAMAP MF_00222
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and PEP: step 4/7. HAMAP MF_00222
Sequence similarities	Belongs to the shikimate dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro shikimate 5-dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 273	273	Shikimate dehydrogenase HAMAP MF_00222		PRO_0000135996
Regions
	Nucleotide binding	131 – 135	5	NADP By similarity
Sites
	Active site	70	1	Proton acceptor Potential
Experimental info
	Sequence conflict	203 – 204	2	LP → T Ref.1
	Sequence conflict	206	1	S → I Ref.1
	Sequence conflict	212	1	T → R in AAD09433. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P46240-1 [UniParc]. Last modified August 30, 2002. Version 2. Checksum: 50507AF854774AF6	FASTA	273	30,885
	10 20 30 40 50 60 MCKLEKFNYA LFGNPIDHSQ SPKIHNFFAT QTGILHIYKA INIPLDQFSS VVSDFFKKNI 70 80 90 100 110 120 KGANVTAPFK KEAYFFSDKL TERAKIAQSV NTLKKISDKC ILGDNTDGIG LLSDLVRLNF 130 140 150 160 170 180 IKKNFSILIL GAGGAVKGVL LPLLSLGCSV YILNRTILNA KILVKQFNKY GKIFVFDRQN 190 200 210 220 230 240 FKQQNFDLVI NAMSRNTEKK NFLPLSLITS KTFFYDMNYS TRNTPFINWC SKAGGSFISN 250 260 270 GIGMLVFQAA YSFLEWHNVL PEINYIINIL NIK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of a putative 23S-5S rRNA operon of Buchnera aphidicola (endosymbiont of aphids) unlinked to the 16S rRNA-encoding gene." Rouhbakhsh D., Baumann P. Gene 155:107-112(1995) [PubMed: 7535281] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	U09230 Genomic DNA. Translation: AAD09433.1. AE013218 Genomic DNA. Translation: AAM68017.1.
RefSeq	NP_660806.1.
3D structure databases
HSSP	HSSP built from PDB template 1NYT based on UniProtKB P15770.
ModBase	Search...
Genome annotation databases
GeneID	1005693.
GenomeReviews	Gene locus BUsg_474 in contig AE013218_GR.
KEGG	bas:BUsg474.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P46240.
OMA	P46240. YDMMYGR.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG474-MON.
Family and domain databases
HAMAP	MF_00222. [Tree]
InterPro	IPR011342. Quinate/shikimate_5-DH. IPR013708. Shikimate_DH-bd_N. IPR006151. Shikm_DH/Glu-tRNA_Rdtase. [Graphical view]
Pfam	PF01488. Shikimate_DH. 1 hit. PF08501. Shikimate_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00507. aroE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AROE_BUCAP

Accession

Primary (citable) accession number: P46240

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	August 30, 2002
Last modified:	June 16, 2009
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)