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Protein

Shikimate dehydrogenase (NADP(+))

Gene

aroE

Organism
Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).UniRule annotation

Catalytic activityi

Shikimate + NADP+ = 3-dehydroshikimate + NADPH.UniRule annotation

Pathway: chorismate biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive (aroH)
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroQ)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei66 – 661ShikimateUniRule annotation
Active sitei70 – 701Proton acceptorUniRule annotation
Binding sitei82 – 821NADPUniRule annotation
Binding sitei91 – 911ShikimateUniRule annotation
Binding sitei107 – 1071ShikimateUniRule annotation
Binding sitei217 – 2171NADP; via carbonyl oxygenUniRule annotation
Binding sitei219 – 2191ShikimateUniRule annotation
Binding sitei241 – 2411NADP; via carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi131 – 1355NADPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciBAPH198804:GHMG-502-MONOMER.
UniPathwayiUPA00053; UER00087.

Names & Taxonomyi

Protein namesi
Recommended name:
Shikimate dehydrogenase (NADP(+))UniRule annotation (EC:1.1.1.25UniRule annotation)
Short name:
SDHUniRule annotation
Gene namesi
Name:aroEUniRule annotation
Ordered Locus Names:BUsg_474
OrganismiBuchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Taxonomic identifieri198804 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000000416 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 273273Shikimate dehydrogenase (NADP(+))PRO_0000135996Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi198804.BUsg474.

Structurei

3D structure databases

ProteinModelPortaliP46240.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni19 – 213Shikimate bindingUniRule annotation

Sequence similaritiesi

Belongs to the shikimate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0169.
KOiK00014.
OMAiSASQIPY.
OrthoDBiEOG6RRKMV.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00222. Shikimate_DH_AroE.
InterProiIPR016040. NAD(P)-bd_dom.
IPR011342. Shikimate_DH.
IPR013708. Shikimate_DH-bd_N.
IPR022893. Shikimate_DH_fam.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view]
PfamiPF01488. Shikimate_DH. 1 hit.
PF08501. Shikimate_dh_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00507. aroE. 1 hit.

Sequencei

Sequence statusi: Complete.

P46240-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCKLEKFNYA LFGNPIDHSQ SPKIHNFFAT QTGILHIYKA INIPLDQFSS
60 70 80 90 100
VVSDFFKKNI KGANVTAPFK KEAYFFSDKL TERAKIAQSV NTLKKISDKC
110 120 130 140 150
ILGDNTDGIG LLSDLVRLNF IKKNFSILIL GAGGAVKGVL LPLLSLGCSV
160 170 180 190 200
YILNRTILNA KILVKQFNKY GKIFVFDRQN FKQQNFDLVI NAMSRNTEKK
210 220 230 240 250
NFLPLSLITS KTFFYDMNYS TRNTPFINWC SKAGGSFISN GIGMLVFQAA
260 270
YSFLEWHNVL PEINYIINIL NIK
Length:273
Mass (Da):30,885
Last modified:August 30, 2002 - v2
Checksum:i50507AF854774AF6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti203 – 2042LP → T in AAD09433 (PubMed:7535281).Curated
Sequence conflicti206 – 2061S → I in AAD09433 (PubMed:7535281).Curated
Sequence conflicti212 – 2121T → R in AAD09433 (PubMed:7535281).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09230 Genomic DNA. Translation: AAD09433.1.
AE013218 Genomic DNA. Translation: AAM68017.1.
RefSeqiNP_660806.1. NC_004061.1.
WP_011053983.1. NC_004061.1.

Genome annotation databases

EnsemblBacteriaiAAM68017; AAM68017; BUsg_474.
KEGGibas:BUsg474.
PATRICi21247812. VBIBucAph100086_0501.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09230 Genomic DNA. Translation: AAD09433.1.
AE013218 Genomic DNA. Translation: AAM68017.1.
RefSeqiNP_660806.1. NC_004061.1.
WP_011053983.1. NC_004061.1.

3D structure databases

ProteinModelPortaliP46240.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi198804.BUsg474.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM68017; AAM68017; BUsg_474.
KEGGibas:BUsg474.
PATRICi21247812. VBIBucAph100086_0501.

Phylogenomic databases

eggNOGiCOG0169.
KOiK00014.
OMAiSASQIPY.
OrthoDBiEOG6RRKMV.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00087.
BioCyciBAPH198804:GHMG-502-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00222. Shikimate_DH_AroE.
InterProiIPR016040. NAD(P)-bd_dom.
IPR011342. Shikimate_DH.
IPR013708. Shikimate_DH-bd_N.
IPR022893. Shikimate_DH_fam.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view]
PfamiPF01488. Shikimate_DH. 1 hit.
PF08501. Shikimate_dh_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00507. aroE. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of a putative 23S-5S rRNA operon of Buchnera aphidicola (endosymbiont of aphids) unlinked to the 16S rRNA-encoding gene."
    Rouhbakhsh D., Baumann P.
    Gene 155:107-112(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Sg.

Entry informationi

Entry nameiAROE_BUCAP
AccessioniPrimary (citable) accession number: P46240
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: August 30, 2002
Last modified: April 29, 2015
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Buchnera aphidicola (subsp. Schizaphis graminum)
    Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.