P46240 (AROE_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Shikimate dehydrogenase
EC=1.1.1.25

Gene names

Name:	aroE
Ordered Locus Names:	BUsg_474

Organism

Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]

Taxonomic identifier

198804 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Schizaphis graminum)

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Shikimate + NADP+ = 3-dehydroshikimate + NADPH. HAMAP-Rule MF_00222
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7. HAMAP-Rule MF_00222
Sequence similarities	Belongs to the shikimate dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP chorismate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway shikimate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	NADP binding Inferred from electronic annotation. Source: InterPro shikimate 3-dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 273	273	Shikimate dehydrogenase HAMAP-Rule MF_00222		PRO_0000135996
Regions
	Nucleotide binding	131 – 135	5	NADP By similarity
Sites
	Active site	70	1	Proton acceptor Potential
Experimental info
	Sequence conflict	203 – 204	2	LP → T in AAD09433. Ref.1
	Sequence conflict	206	1	S → I in AAD09433. Ref.1
	Sequence conflict	212	1	T → R in AAD09433. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P46240 [UniParc]. Last modified August 30, 2002. Version 2. Checksum: 50507AF854774AF6	FASTA	273	30,885
	10 20 30 40 50 60 MCKLEKFNYA LFGNPIDHSQ SPKIHNFFAT QTGILHIYKA INIPLDQFSS VVSDFFKKNI 70 80 90 100 110 120 KGANVTAPFK KEAYFFSDKL TERAKIAQSV NTLKKISDKC ILGDNTDGIG LLSDLVRLNF 130 140 150 160 170 180 IKKNFSILIL GAGGAVKGVL LPLLSLGCSV YILNRTILNA KILVKQFNKY GKIFVFDRQN 190 200 210 220 230 240 FKQQNFDLVI NAMSRNTEKK NFLPLSLITS KTFFYDMNYS TRNTPFINWC SKAGGSFISN 250 260 270 GIGMLVFQAA YSFLEWHNVL PEINYIINIL NIK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of a putative 23S-5S rRNA operon of Buchnera aphidicola (endosymbiont of aphids) unlinked to the 16S rRNA-encoding gene." Rouhbakhsh D., Baumann P. Gene 155:107-112(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sg.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U09230 Genomic DNA. Translation: AAD09433.1. AE013218 Genomic DNA. Translation: AAM68017.1.
RefSeq	NP_660806.1. NC_004061.1.
3D structure databases
ProteinModelPortal	P46240.
ModBase	Search...
Protein-protein interaction databases
STRING	198804.BUsg474.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM68017; AAM68017; BUsg_474.
GeneID	1005693.
KEGG	bas:BUsg474.
PATRIC	21247812. VBIBucAph100086_0501.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0169.
KO	K00014.
OMA	AFDLIIN.
ProtClustDB	PRK00258.
Enzyme and pathway databases
BioCyc	BAPH198804:GHMG-519-MONOMER.
UniPathway	UPA00053; UER00087.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
HAMAP	MF_00222. Shikimate_DH_AroE.
InterPro	IPR016040. NAD(P)-bd_dom. IPR011342. Shikimate_DH. IPR013708. Shikimate_DH-bd_N. IPR022893. Shikimate_quinate_DH. IPR006151. Shikm_DH/Glu-tRNA_Rdtase. [Graphical view]
Pfam	PF01488. Shikimate_DH. 1 hit. PF08501. Shikimate_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00507. aroE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AROE_BUCAP

Accession

Primary (citable) accession number: P46240

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	August 30, 2002
Last modified:	May 1, 2013
This is version 97 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program