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Protein

Shikimate dehydrogenase (NADP(+))

Gene

aroE

Organism
Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).UniRule annotation

Catalytic activityi

Shikimate + NADP+ = 3-dehydroshikimate + NADPH.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive (aroH)
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroQ)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei66ShikimateUniRule annotation1
Active sitei70Proton acceptorUniRule annotation1
Binding sitei82NADPUniRule annotation1
Binding sitei91ShikimateUniRule annotation1
Binding sitei107ShikimateUniRule annotation1
Binding sitei217NADP; via carbonyl oxygenUniRule annotation1
Binding sitei219ShikimateUniRule annotation1
Binding sitei241NADP; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi131 – 135NADPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis
LigandNADP

Enzyme and pathway databases

BioCyciBAPH198804:G1FZU-504-MONOMER
UniPathwayiUPA00053; UER00087

Names & Taxonomyi

Protein namesi
Recommended name:
Shikimate dehydrogenase (NADP(+))UniRule annotation (EC:1.1.1.25UniRule annotation)
Short name:
SDHUniRule annotation
Gene namesi
Name:aroEUniRule annotation
Ordered Locus Names:BUsg_474
OrganismiBuchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Taxonomic identifieri198804 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesErwiniaceaeBuchnera
Proteomesi
  • UP000000416 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001359961 – 273Shikimate dehydrogenase (NADP(+))Add BLAST273

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi198804.BUsg474

Structurei

3D structure databases

ProteinModelPortaliP46240
SMRiP46240
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni19 – 21Shikimate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the shikimate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105E2X Bacteria
COG0169 LUCA
KOiK00014
OMAiFGNPIKH
OrthoDBiPOG091H02DY

Family and domain databases

HAMAPiMF_00222 Shikimate_DH_AroE, 1 hit
InterProiView protein in InterPro
IPR036291 NAD(P)-bd_dom_sf
IPR011342 Shikimate_DH
IPR013708 Shikimate_DH-bd_N
IPR022893 Shikimate_DH_fam
IPR006151 Shikm_DH/Glu-tRNA_Rdtase
PfamiView protein in Pfam
PF01488 Shikimate_DH, 1 hit
PF08501 Shikimate_dh_N, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR00507 aroE, 1 hit

Sequencei

Sequence statusi: Complete.

P46240-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCKLEKFNYA LFGNPIDHSQ SPKIHNFFAT QTGILHIYKA INIPLDQFSS
60 70 80 90 100
VVSDFFKKNI KGANVTAPFK KEAYFFSDKL TERAKIAQSV NTLKKISDKC
110 120 130 140 150
ILGDNTDGIG LLSDLVRLNF IKKNFSILIL GAGGAVKGVL LPLLSLGCSV
160 170 180 190 200
YILNRTILNA KILVKQFNKY GKIFVFDRQN FKQQNFDLVI NAMSRNTEKK
210 220 230 240 250
NFLPLSLITS KTFFYDMNYS TRNTPFINWC SKAGGSFISN GIGMLVFQAA
260 270
YSFLEWHNVL PEINYIINIL NIK
Length:273
Mass (Da):30,885
Last modified:August 30, 2002 - v2
Checksum:i50507AF854774AF6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti203 – 204LP → T in AAD09433 (PubMed:7535281).Curated2
Sequence conflicti206S → I in AAD09433 (PubMed:7535281).Curated1
Sequence conflicti212T → R in AAD09433 (PubMed:7535281).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09230 Genomic DNA Translation: AAD09433.1
AE013218 Genomic DNA Translation: AAM68017.1
RefSeqiWP_011053983.1, NC_004061.1

Genome annotation databases

EnsemblBacteriaiAAM68017; AAM68017; BUsg_474
KEGGibas:BUsg_474

Similar proteinsi

Entry informationi

Entry nameiAROE_BUCAP
AccessioniPrimary (citable) accession number: P46240
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: August 30, 2002
Last modified: March 28, 2018
This is version 122 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health