Reviewed, UniProtKB/Swiss-Prot P46239 (GUNF_FUSOX)

Names and origin

Protein names	Recommended name:     Putative endoglucanase type F     EC=3.2.1.4 Alternative name(s):     Endo-1,4-beta-glucanase     Cellulase
Organism	Fusarium oxysporum
Taxonomic identifier	5507 [NCBI]
Taxonomic lineage	Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Hypocreomycetidae › Hypocreales › mitosporic Hypocreales › Fusarium › Fusarium oxysporum species complex

Protein attributes

Sequence length	385 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Sequence similarities	Belongs to the glycosyl hydrolase 10 (cellulase F) family. Contains 1 CBM1 (fungal-type carbohydrate-binding) domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Domain	Signal
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: InterPro
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro cellulase activity Inferred from electronic annotation. Source: EC cellulose binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 385	366	Putative endoglucanase type F		PRO_0000007961
Regions
	Domain	23 – 53	31	CBM1
	Region	53 – 84	32	Linker
	Region	85 – 385	301	Catalytic
Sites
	Active site	210	1	Proton donor By similarity
	Active site	321	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	P46239-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: B3C3807C07D3C0EC	FASTA	385	41,225
	10 20 30 40 50 60 MHTLSVLLAL APVSALAQAP IWGQCGGNGW TGATTCASGL KCEKINDWYY QCVPGSGGSE 70 80 90 100 110 120 PQPSSTQGGG TPQPTGGNSG GTGLDAKFKA KGKQYFGTEI DHYHLNNNPL INIVKAQFGQ 130 140 150 160 170 180 VTCENSMKWD AIEPSRNSFT FSNADKVVDF ATQNGKLIRG HTLLWHSQLP QWVQNINDRS 190 200 210 220 230 240 TLTAVIENHV KTMVTRYKGK ILQWDVVNNE IFAEDGNLRD SVFSRVLGED FVGIAFRAAR 250 260 270 280 290 300 AADPAAKLYI NDYNLDKSDY AKVTRGMVAH VNKWIAAGIP IDGIGSQGHL AAPSGWNPAS 310 320 330 340 350 360 GVPAALRALA ASDAKEIAIT ELDIAGASAN DYLTVMNACL AVPKCVGITV WGVSDKDSWR 370 380 PGDNPLLYDS NYQPKAAFNA LANAL

References

[1]

"The use of conserved cellulase family-specific sequences to clone cellulase homologue cDNAs from Fusarium oxysporum." Sheppard P.O., Grant F.J., Oort P.J., Sprecher C.A., Foster D.C., Hagen F.S., Upshall A., McKnight G.L., O'Hara P.J. Gene 150:163-167(1994) [PubMed: 7959045] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	L29380 mRNA. Translation: AAA65588.1.
3D structure databases
HSSP	HSSP built from PDB template 1B31 based on UniProtKB P56588.
ModBase	Search...
Protein family/group databases
CAZy	CBM1. Carbohydrate-Binding Module Family 1. GH10. Glycoside Hydrolase Family 10.
Enzyme and pathway databases
BRENDA	3.2.1.4. 15244.
Family and domain databases
InterPro	IPR000254. CBD_fun. IPR001000. Glyco_hydro_10. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00734. CBM_1. 1 hit. PF00331. Glyco_hydro_10. 1 hit. [Graphical view]
PRINTS	PR00134. GLHYDRLASE10.
ProDom	PD001821. CBD_fungal. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00236. fCBD. 1 hit. SM00633. Glyco_10. 1 hit. [Graphical view]
PROSITE	PS00562. CBM1_1. 1 hit. PS51164. CBM1_2. 1 hit. PS00591. GLYCOSYL_HYDROL_F10. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUNF_FUSOX

Accession

Primary (citable) accession number: P46239

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)