Reviewed, UniProtKB/Swiss-Prot P46238 (GUXC_FUSOX)

Names and origin

Protein names	Recommended name:     Putative exoglucanase type C     EC=3.2.1.91 Alternative name(s):     Exocellobiohydrolase I     1,4-beta-cellobiohydrolase     Beta-glucancellobiohydrolase
Organism	Fusarium oxysporum
Taxonomic identifier	5507 [NCBI]
Taxonomic lineage	Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Hypocreomycetidae › Hypocreales › mitosporic Hypocreales › Fusarium › Fusarium oxysporum species complex

Protein attributes

Sequence length	514 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Hydrolysis of 1,4-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains.
Sequence similarities	Belongs to the glycosyl hydrolase 7 (cellulase C) family. Contains 1 CBM1 (fungal-type carbohydrate-binding) domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: InterPro
Molecular function	cellulose 1,4-beta-cellobiosidase activity Inferred from electronic annotation. Source: EC cellulose binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 514	497	Putative exoglucanase type C		PRO_0000007913
Regions
	Domain	478 – 514	37	CBM1
	Region	18 – 439	422	Catalytic
	Region	440 – 482	43	Linker
Sites
	Active site	229	1	Nucleophile By similarity
	Active site	234	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	287	1	N-linked (GlcNAc...) Potential
	Glycosylation	490	1	N-linked (GlcNAc...) Potential
	Disulfide bond	486 ↔ 503		By similarity
	Disulfide bond	497 ↔ 513		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P46238-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 6A4617323A46E062	FASTA	514	54,704
	10 20 30 40 50 60 MYRIVATASA LIAAARAQQV CSLNTETKPA LTWSKCTSSG CSDVKGSVVI DANWRWTHQT 70 80 90 100 110 120 SGSTNCYTGN KWDTSICTDG KTCAEKCCLD GADYSGTYGI TSSGNQLSLG FVTNGPYSKN 130 140 150 160 170 180 IGSRTYLMEN ENTYQMFQLL GNEFTFDVDV SGIGCGLNGA PHFVSMDEDG GKAKYSGNKA 190 200 210 220 230 240 GAKYGTGYCD AQCPRDVKFI NGVANSEGWK PSDSDVNAGV GNLGTCCPEM DIWEANSIST 250 260 270 280 290 300 AFTPHPCTKL TQHSCTGDSC GGTYSSDRYG GTCDADGCDF NAYRQGNKTF YGPGSNFNID 310 320 330 340 350 360 TTKKMTVVTQ FHKGSNGRLS EITRLYVQNG KVIANSESKI AGNPGSSLTS DFCSKQKSVF 370 380 390 400 410 420 GDIDDFSKKG GWNGMSDALS APMVLVMSLW HDHHSNMLWL DSTYPTDSTK VGSQRGSCAT 430 440 450 460 470 480 TSGKPSDLER DVPNSKVSFS NIKFGPIGST YKSDGTTPNP PASSSTTGSS TPTNPPAGSV 490 500 510 DQWGQCGGQN YSGPTTCKSP FTCKKINDFY SQCQ

References

[1]

"The use of conserved cellulase family-specific sequences to clone cellulase homologue cDNAs from Fusarium oxysporum." Sheppard P.O., Grant F.J., Oort P.J., Sprecher C.A., Foster D.C., Hagen F.S., Upshall A., McKnight G.L., O'Hara P.J. Gene 150:163-167(1994) [PubMed: 7959045] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	L29379 mRNA. Translation: AAA65587.1.
3D structure databases
HSSP	HSSP built from PDB template 6CEL based on UniProtKB P00725.
ModBase	Search...
Protein family/group databases
CAZy	CBM1. Carbohydrate-Binding Module Family 1. GH7. Glycoside Hydrolase Family 7.
Enzyme and pathway databases
BRENDA	3.2.1.91. 15244.
Family and domain databases
InterPro	IPR000254. CBD_fun. IPR001722. Glyco_hydro_7. [Graphical view]
Gene3D	G3DSA:2.70.100.10. Glyco_hydro_7. 1 hit.
Pfam	PF00734. CBM_1. 1 hit. PF00840. Glyco_hydro_7. 1 hit. [Graphical view]
PRINTS	PR00734. GLHYDRLASE7.
ProDom	PD001821. CBD_fungal. 1 hit. PD186135. Glyco_hydro_7. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00236. fCBD. 1 hit. [Graphical view]
PROSITE	PS00562. CBM1_1. 1 hit. PS51164. CBM1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUXC_FUSOX

Accession

Primary (citable) accession number: P46238

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 14, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)