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P46236 (GUNB_FUSOX) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative endoglucanase type B

EC=3.2.1.4
Alternative name(s):
Cellulase
Endo-1,4-beta-glucanase
OrganismFusarium oxysporum (Fusarium vascular wilt)
Taxonomic identifier5507 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesNectriaceaeFusariumFusarium oxysporum species complex

Protein attributes

Sequence length462 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sequence similarities

Belongs to the glycosyl hydrolase 6 (cellulase B) family.

Contains 1 CBM1 (fungal-type carbohydrate-binding) domain.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Cellulose degradation
Polysaccharide degradation
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
Glycoprotein
Gene Ontology (GO)
   Biological_processcellulose catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: InterPro

   Molecular_functioncellulase activity

Inferred from electronic annotation. Source: UniProtKB-EC

cellulose binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1616 Potential
Chain17 – 462446Putative endoglucanase type B
PRO_0000007910

Regions

Domain25 – 6137CBM1
Region66 – 9934Linker
Region100 – 462363Catalytic

Sites

Active site1901 By similarity
Active site2361Proton donor By similarity
Active site4161Nucleophile By similarity

Amino acid modifications

Glycosylation371N-linked (GlcNAc...) Potential
Glycosylation2231N-linked (GlcNAc...) Potential
Glycosylation2721N-linked (GlcNAc...) Potential
Glycosylation3171N-linked (GlcNAc...) Potential
Disulfide bond33 ↔ 50 By similarity
Disulfide bond44 ↔ 60 By similarity
Disulfide bond191 ↔ 250 By similarity
Disulfide bond383 ↔ 430 By similarity

Sequences

Sequence LengthMass (Da)Tools
P46236 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: E25B2F5B828B637F

FASTA46249,208
        10         20         30         40         50         60 
MAYKLILAAF AATALAAPVE ERQSCSNGVW AQCGGQNWSG TPCCTSGNKC VKLNDFYSQC 

        70         80         90        100        110        120 
QPGSAEPSST AAGPSSTTAT KTTATGGSST TAGGSVTSAP PAASDNPYAG VDLWANNYYR 

       130        140        150        160        170        180 
SEVMNLAVPK LSGAKATAAA KVADVPSFQW MDTYDHISLM EDTLADIRKA NKAGGKYAGQ 

       190        200        210        220        230        240 
FVVYDLPNRD CAAAASNGEY SLDKDGANKY KAYIAKIKGI LQNYSDTKVI LVIEPDSLAN 

       250        260        270        280        290        300 
LVTNLNVDKC AKAESAYKEL TVYAIKELNL PNVSMYLDAG HGGWLGWPAN IGPAAKLYAQ 

       310        320        330        340        350        360 
IYKDAGKPSR VRGLVTNVSN YNGWKLSTKP DYTESNPNYD EQRYINAFAP LLAQEGWSNV 

       370        380        390        400        410        420 
KFIVDQGRSG KQPTGQKAQG DWCNAKGTGF GLRPSTNTGD ALADAFVWVK PGGESDGTSD 

       430        440        450        460 
TSAARYDYHC GLDDALKPAP EAGTWFQAYF EQLLDNANPS FL 

« Hide

References

[1]"The use of conserved cellulase family-specific sequences to clone cellulase homologue cDNAs from Fusarium oxysporum."
Sheppard P.O., Grant F.J., Oort P.J., Sprecher C.A., Foster D.C., Hagen F.S., Upshall A., McKnight G.L., O'Hara P.J.
Gene 150:163-167(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L29377 mRNA. Translation: AAA65585.1.

3D structure databases

ProteinModelPortalP46236.
SMRP46236. Positions 106-461.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyCBM1. Carbohydrate-Binding Module Family 1.
GH6. Glycoside Hydrolase Family 6.

Proteomic databases

PRIDEP46236.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.20.20.40. 1 hit.
InterProIPR016288. Beta_cellobiohydrolase.
IPR000254. Cellulose-bd_dom_fun.
IPR001524. Glyco_hydro_6_CS.
[Graphical view]
PfamPF00734. CBM_1. 1 hit.
PF01341. Glyco_hydro_6. 1 hit.
[Graphical view]
PIRSFPIRSF001100. Beta_cellobiohydrolase. 1 hit.
PRINTSPR00733. GLHYDRLASE6.
ProDomPD001821. CBD_fun. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00236. fCBD. 1 hit.
[Graphical view]
SUPFAMSSF51989. SSF51989. 1 hit.
SSF57180. SSF57180. 1 hit.
PROSITEPS00562. CBM1_1. 1 hit.
PS51164. CBM1_2. 1 hit.
PS00655. GLYCOSYL_HYDROL_F6_1. 1 hit.
PS00656. GLYCOSYL_HYDROL_F6_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGUNB_FUSOX
AccessionPrimary (citable) accession number: P46236
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 14, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries