P46236 (GUNB_FUSOX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Putative endoglucanase type B EC=3.2.1.4 Alternative name(s): Cellulase Endo-1,4-beta-glucanase
Organism	Fusarium oxysporum (Panama disease fungus)
Taxonomic identifier	5507 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › sordariomyceta › Sordariomycetes › Hypocreomycetidae › Hypocreales › Nectriaceae › mitosporic Nectriaceae › Fusarium › Fusarium oxysporum species complex

Protein attributes

Sequence length	462 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Sequence similarities	Belongs to the glycosyl hydrolase 6 (cellulase B) family. Contains 1 CBM1 (fungal-type carbohydrate-binding) domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: InterPro
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC cellulose binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Chain	17 – 462	446	Putative endoglucanase type B		PRO_0000007910
Regions
	Domain	25 – 61	37	CBM1
	Region	66 – 99	34	Linker
	Region	100 – 462	363	Catalytic
Sites
	Active site	190	1	By similarity
	Active site	236	1	Proton donor By similarity
	Active site	416	1	Nucleophile By similarity
Amino acid modifications
	Glycosylation	37	1	N-linked (GlcNAc...) Potential
	Glycosylation	223	1	N-linked (GlcNAc...) Potential
	Glycosylation	272	1	N-linked (GlcNAc...) Potential
	Glycosylation	317	1	N-linked (GlcNAc...) Potential
	Disulfide bond	33 ↔ 50		By similarity
	Disulfide bond	44 ↔ 60		By similarity
	Disulfide bond	191 ↔ 250		By similarity
	Disulfide bond	383 ↔ 430		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P46236 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: E25B2F5B828B637F	FASTA	462	49,208
	10 20 30 40 50 60 MAYKLILAAF AATALAAPVE ERQSCSNGVW AQCGGQNWSG TPCCTSGNKC VKLNDFYSQC 70 80 90 100 110 120 QPGSAEPSST AAGPSSTTAT KTTATGGSST TAGGSVTSAP PAASDNPYAG VDLWANNYYR 130 140 150 160 170 180 SEVMNLAVPK LSGAKATAAA KVADVPSFQW MDTYDHISLM EDTLADIRKA NKAGGKYAGQ 190 200 210 220 230 240 FVVYDLPNRD CAAAASNGEY SLDKDGANKY KAYIAKIKGI LQNYSDTKVI LVIEPDSLAN 250 260 270 280 290 300 LVTNLNVDKC AKAESAYKEL TVYAIKELNL PNVSMYLDAG HGGWLGWPAN IGPAAKLYAQ 310 320 330 340 350 360 IYKDAGKPSR VRGLVTNVSN YNGWKLSTKP DYTESNPNYD EQRYINAFAP LLAQEGWSNV 370 380 390 400 410 420 KFIVDQGRSG KQPTGQKAQG DWCNAKGTGF GLRPSTNTGD ALADAFVWVK PGGESDGTSD 430 440 450 460 TSAARYDYHC GLDDALKPAP EAGTWFQAYF EQLLDNANPS FL

References

[1]

"The use of conserved cellulase family-specific sequences to clone cellulase homologue cDNAs from Fusarium oxysporum." Sheppard P.O., Grant F.J., Oort P.J., Sprecher C.A., Foster D.C., Hagen F.S., Upshall A., McKnight G.L., O'Hara P.J. Gene 150:163-167(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	L29377 mRNA. Translation: AAA65585.1.
3D structure databases
ProteinModelPortal	P46236.
SMR	P46236. Positions 106-461.
ModBase	Search...
Protein family/group databases
CAZy	CBM1. Carbohydrate-Binding Module Family 1. GH6. Glycoside Hydrolase Family 6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.20.20.40. 1 hit.
InterPro	IPR016288. Beta_cellobiohydrolase. IPR000254. Cellulose-bd_dom_fun. IPR001524. Glyco_hydro_6_CS. [Graphical view]
Pfam	PF00734. CBM_1. 1 hit. PF01341. Glyco_hydro_6. 1 hit. [Graphical view]
PIRSF	PIRSF001100. Beta_cellobiohydrolase. 1 hit.
PRINTS	PR00733. GLHYDRLASE6.
ProDom	PD001821. CBD_fun. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00236. fCBD. 1 hit. [Graphical view]
SUPFAM	SSF57180. CBD_fun. 1 hit. SSF51989. Glyco_hydro_6. 1 hit.
PROSITE	PS00562. CBM1_1. 1 hit. PS51164. CBM1_2. 1 hit. PS00655. GLYCOSYL_HYDROL_F6_1. 1 hit. PS00656. GLYCOSYL_HYDROL_F6_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUNB_FUSOX

Accession

Primary (citable) accession number: P46236

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program