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P46235 (LGUL_VIBPA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable lactoylglutathione lyase

EC=4.4.1.5
Alternative name(s):
Aldoketomutase
Glyoxalase I
Short name=Glx I
Ketone-aldehyde mutase
Methylglyoxalase
S-D-lactoylglutathione methylglyoxal lyase
Gene names
Name:gloA
Ordered Locus Names:VP2109
OrganismVibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633) [Complete proteome] [HAMAP]
Taxonomic identifier223926 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Protein attributes

Sequence length138 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione By similarity.

Catalytic activity

(R)-S-lactoylglutathione = glutathione + methylglyoxal.

Cofactor

Binds 1 nickel ion per subunit By similarity.

Pathway

Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 1/2.

Sequence similarities

Belongs to the glyoxalase I family.

Ontologies

Keywords
   LigandMetal-binding
Nickel
   Molecular functionLyase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functionlactoylglutathione lyase activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 138138Probable lactoylglutathione lyase
PRO_0000168098

Sites

Active site1251Proton donor/acceptor By similarity
Metal binding81Nickel; via tele nitrogen By similarity
Metal binding591Nickel By similarity
Metal binding771Nickel; via tele nitrogen By similarity
Metal binding1251Nickel By similarity
Binding site121Substrate By similarity
Binding site631Substrate By similarity
Binding site771Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
P46235 [UniParc].

Last modified April 4, 2003. Version 2.
Checksum: 5738D502E3CA35FC

FASTA13815,034
        10         20         30         40         50         60 
MSNGRILHTM LRVGDLDKSI KFYTEVMGMQ LLRTNENKEY EYTLAFVGYG DESQGAVIEL 

        70         80         90        100        110        120 
TYNWGKTEYD LGTAFGHIAI GVDDIYATCD AIKAAGGNVT REAGPVKGGT THIAFVKDPD 

       130 
GYMIELIQNK QASAGLEG 

« Hide

References

« Hide 'large scale' references
[1]"Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism distinct from that of V. cholerae."
Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K., Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S., Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T.
Lancet 361:743-749(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: RIMD 2210633.
[2]"MotY, a component of the sodium-type flagellar motor."
McCarter L.L.
J. Bacteriol. 176:4219-4225(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 6-138.
Strain: BB22.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000031 Genomic DNA. Translation: BAC60372.1.
U06949 Genomic DNA. Translation: AAA21576.1.
RefSeqNP_798488.1. NC_004603.1.

3D structure databases

ProteinModelPortalP46235.
SMRP46235. Positions 5-128.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING223926.VP2109.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC60372; BAC60372; BAC60372.
GeneID1189621.
KEGGvpa:VP2109.
PATRIC20142378. VBIVibPar50997_2019.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0346.
HOGENOMHOG000232011.
KOK01759.
OMAIGEEDIY.
OrthoDBEOG6DVJXX.

Enzyme and pathway databases

BioCycVPAR223926:GHK4-2165-MONOMER.
UniPathwayUPA00619; UER00675.

Family and domain databases

Gene3D3.10.180.10. 1 hit.
InterProIPR029068. Glyas_Bleomycin-R_OHBP_Dase.
IPR004360. Glyas_Fos-R_dOase_dom.
IPR004361. Glyoxalase_1.
IPR018146. Glyoxalase_1_CS.
[Graphical view]
PfamPF00903. Glyoxalase. 1 hit.
[Graphical view]
SUPFAMSSF54593. SSF54593. 1 hit.
TIGRFAMsTIGR00068. glyox_I. 1 hit.
PROSITEPS00934. GLYOXALASE_I_1. 1 hit.
PS00935. GLYOXALASE_I_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLGUL_VIBPA
AccessionPrimary (citable) accession number: P46235
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: April 4, 2003
Last modified: June 11, 2014
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways