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Protein

Probable lactoylglutathione lyase

Gene

gloA

Organism
Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione.By similarity

Catalytic activityi

(R)-S-lactoylglutathione = glutathione + methylglyoxal.

Cofactori

Ni2+By similarityNote: Binds 1 nickel ion per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi8 – 81Nickel; via tele nitrogenBy similarity
Binding sitei12 – 121SubstrateBy similarity
Metal bindingi59 – 591NickelBy similarity
Binding sitei63 – 631SubstrateBy similarity
Metal bindingi77 – 771Nickel; via tele nitrogenBy similarity
Binding sitei77 – 771SubstrateBy similarity
Active sitei125 – 1251Proton donor/acceptorBy similarity
Metal bindingi125 – 1251NickelBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Metal-binding, Nickel

Enzyme and pathway databases

BioCyciVPAR223926:GHK4-2165-MONOMER.
UniPathwayiUPA00619; UER00675.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable lactoylglutathione lyase (EC:4.4.1.5)
Alternative name(s):
Aldoketomutase
Glyoxalase I
Short name:
Glx I
Ketone-aldehyde mutase
Methylglyoxalase
S-D-lactoylglutathione methylglyoxal lyase
Gene namesi
Name:gloA
Ordered Locus Names:VP2109
OrganismiVibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
Taxonomic identifieri223926 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
ProteomesiUP000002493 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 138138Probable lactoylglutathione lyasePRO_0000168098Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi223926.VP2109.

Structurei

3D structure databases

ProteinModelPortaliP46235.
SMRiP46235. Positions 5-128.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glyoxalase I family.Curated

Phylogenomic databases

eggNOGiCOG0346.
HOGENOMiHOG000232011.
KOiK01759.
OMAiKYRLAFV.
OrthoDBiEOG6DVJXX.

Family and domain databases

Gene3Di3.10.180.10. 1 hit.
InterProiIPR029068. Glyas_Bleomycin-R_OHBP_Dase.
IPR004360. Glyas_Fos-R_dOase_dom.
IPR004361. Glyoxalase_1.
IPR018146. Glyoxalase_1_CS.
[Graphical view]
PfamiPF00903. Glyoxalase. 1 hit.
[Graphical view]
SUPFAMiSSF54593. SSF54593. 1 hit.
TIGRFAMsiTIGR00068. glyox_I. 1 hit.
PROSITEiPS00934. GLYOXALASE_I_1. 1 hit.
PS00935. GLYOXALASE_I_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P46235-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNGRILHTM LRVGDLDKSI KFYTEVMGMQ LLRTNENKEY EYTLAFVGYG
60 70 80 90 100
DESQGAVIEL TYNWGKTEYD LGTAFGHIAI GVDDIYATCD AIKAAGGNVT
110 120 130
REAGPVKGGT THIAFVKDPD GYMIELIQNK QASAGLEG
Length:138
Mass (Da):15,034
Last modified:April 4, 2003 - v2
Checksum:i5738D502E3CA35FC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000031 Genomic DNA. Translation: BAC60372.1.
U06949 Genomic DNA. Translation: AAA21576.1.
RefSeqiNP_798488.1. NC_004603.1.
WP_005480812.1. NC_004603.1.

Genome annotation databases

EnsemblBacteriaiBAC60372; BAC60372; BAC60372.
GeneIDi1189621.
KEGGivpa:VP2109.
PATRICi20142378. VBIVibPar50997_2019.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000031 Genomic DNA. Translation: BAC60372.1.
U06949 Genomic DNA. Translation: AAA21576.1.
RefSeqiNP_798488.1. NC_004603.1.
WP_005480812.1. NC_004603.1.

3D structure databases

ProteinModelPortaliP46235.
SMRiP46235. Positions 5-128.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi223926.VP2109.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC60372; BAC60372; BAC60372.
GeneIDi1189621.
KEGGivpa:VP2109.
PATRICi20142378. VBIVibPar50997_2019.

Phylogenomic databases

eggNOGiCOG0346.
HOGENOMiHOG000232011.
KOiK01759.
OMAiKYRLAFV.
OrthoDBiEOG6DVJXX.

Enzyme and pathway databases

UniPathwayiUPA00619; UER00675.
BioCyciVPAR223926:GHK4-2165-MONOMER.

Family and domain databases

Gene3Di3.10.180.10. 1 hit.
InterProiIPR029068. Glyas_Bleomycin-R_OHBP_Dase.
IPR004360. Glyas_Fos-R_dOase_dom.
IPR004361. Glyoxalase_1.
IPR018146. Glyoxalase_1_CS.
[Graphical view]
PfamiPF00903. Glyoxalase. 1 hit.
[Graphical view]
SUPFAMiSSF54593. SSF54593. 1 hit.
TIGRFAMsiTIGR00068. glyox_I. 1 hit.
PROSITEiPS00934. GLYOXALASE_I_1. 1 hit.
PS00935. GLYOXALASE_I_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism distinct from that of V. cholerae."
    Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K., Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S., Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T.
    Lancet 361:743-749(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: RIMD 2210633.
  2. "MotY, a component of the sodium-type flagellar motor."
    McCarter L.L.
    J. Bacteriol. 176:4219-4225(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 6-138.
    Strain: BB22.

Entry informationi

Entry nameiLGUL_VIBPA
AccessioniPrimary (citable) accession number: P46235
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: April 4, 2003
Last modified: May 27, 2015
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.