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Reviewed, UniProtKB/Swiss-Prot P46225 (TPIC_SECCE)

Last modified June 16, 2009. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Triosephosphate isomerase, chloroplastic
      Short name=TIM
      Short name=Triose-phosphate isomerase
    EC=5.3.1.1
OrganismSecale cereale (Rye)
Taxonomic identifier4550 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Secale

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Protein attributes

Sequence length298 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Catalytic activity

D-glyceraldehyde 3-phosphate = glycerone phosphate.

Pathway

Carbohydrate biosynthesis; Calvin cycle.

Subunit structure

Homodimer By similarity.

Subcellular location

Plastid › chloroplast.

Miscellaneous

In plants, there are two types of TPIS, cytosolic and plastid.

Sequence similarities

Belongs to the triosephosphate isomerase family.

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Ontologies

Keywords
   Biological processCalvin cycle
   Cellular componentChloroplast
Plastid
   DomainTransit peptide
   Molecular functionIsomerase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological processreductive pentose-phosphate cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentchloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functiontriose-phosphate isomerase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4343Chloroplast Ref.1
Chain44 – 298255Triosephosphate isomerase, chloroplastic
PRO_0000035651

Sites

Active site1381Electrophile By similarity
Active site2081Proton acceptor By similarity
Binding site541Substrate By similarity
Binding site561Substrate By similarity

Amino acid modifications

Modified residue1861Cysteine derivative

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Sequences

Sequence LengthMass (Da)Tools
P46225-1 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: C5B9A42227E5B750

FASTA29831,633
        10         20         30         40         50         60 
MAARRPSPPP ASPPPPRPRS TTTTRTTSSA SAAPAAAQRL VAMAGSGKFF VGGNWKCNGT 

        70         80         90        100        110        120 
KESISKLVSD LNAATLESDV DVVVAPPFIY IDQVKSSLTD RIEVSAQNTW IGKGGAFTGE 

       130        140        150        160        170        180 
ISAEQLVDIG CQWVILGHSE RRHVIGEDDE FIGKKAAYAL SQNLKVMACI GELLEEREAG 

       190        200        210        220        230        240 
KTFDVCFKQM KAFADNITDW TNVVIAYEPV WAIGTGKVAS PEQAQEVHAA VRDWLKTNVS 

       250        260        270        280        290 
ADVASTVRII YGGSVNAANC AELAKKEDID GFLVGGASLK GPDFATICNS VTSKKVTA

« Hide

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References

[1]"Analysis of the primary structure of the chloroplast isozyme of triosephosphate isomerase from rye leaves by protein and cDNA sequencing indicates a eukaryotic origin of its gene."
Schmidt M., Svendsen I., Feierabend J.
Biochim. Biophys. Acta 1261:257-264(1995) [PubMed: 7711069] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 44-297.
Strain: cv. Halo.
Tissue: Leaf.

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Cross-references

Sequence databases

Z32521 mRNA. Translation: CAA83533.1.
PIRS53761.

3D structure databases

HSSPHSSP built from PDB template 1MO0 based on UniProtKB Q10657.
ModBaseSearch...

Organism-specific databases

GrameneP46225.

Enzyme and pathway databases

BRENDA5.3.1.1. 1472.

Family and domain databases

InterProIPR013785. Aldolase_TIM.
IPR000652. Triosephosphate_isomerase.
[Graphical view]
Gene3DG3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHERPTHR21139. Triophos_ismrse. 1 hit.
PfamPF00121. TIM. 1 hit.
[Graphical view]
ProDomPD001005. Triophos_ismrse. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsTIGR00419. tim. 1 hit.
PROSITEPS00171. TIM_1. 1 hit.
PS51440. TIM_2. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameTPIC_SECCE
AccessionPrimary (citable) accession number: P46225
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 16, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents