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P46215

- SYI_SULAC

UniProt

P46215 - SYI_SULAC

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei597 – 5971ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSACI330779:GH9J-591-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Saci_0599
OrganismiSulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Taxonomic identifieri330779 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
ProteomesiUP000001018: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10501050Isoleucine--tRNA ligasePRO_0000098592Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi330779.Saci_0599.

Structurei

3D structure databases

ProteinModelPortaliP46215.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi45 – 5612"HIGH" regionAdd
BLAST
Motifi594 – 5985"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiKWIISEI.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P46215-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTSKFDPLKF EEEVLKYWDD NNIYKKLKEI NEKNHKKFLF IDGPPYPSSP
60 70 80 90 100
IPHIGTVWNK TIKDCILRYE RLMGYSVKDQ PGYDTHGLPI EVETEKRLGI
110 120 130 140 150
KSKAEIIEKV GVDNFISKCK EFAVNNSKSL TQNFRNLGIF MDWENPYFTF
160 170 180 190 200
NNDYISNSWA VIKKAYERGL LYKGVHVLHW CSRCETTLAD YEVSEYRDLE
210 220 230 240 250
DPSIYVKFRV KGEPNRYLVI WTTTPWTLPA NVFVMINKDF EYADVRVGDE
260 270 280 290 300
ILVIAKDRVK ELMKEARIKE YKILRVYKGE ELLGLEYEHP LADIVSAQSK
310 320 330 340 350
INNHHKVLDG GEAVTLQEGT GLVHSAPGHG DVDFEIGKKY DMPVVMLVND
360 370 380 390 400
KGEFTQDSGK YAGKYVRSAS EEIISDLKQR SALLHASKIV HRYPVCWRCK
410 420 430 440 450
TPLILRAIEQ WFIAVSKLKD HLMGEIDRVR WIPDWGKTRI GNMVKEVRDW
460 470 480 490 500
VISRQRFWGT PLPIWVCSNC QNIIVVGGVD ELSKISINQV PQDLHRPWID
510 520 530 540 550
SVVVRCEKCG GEARRISDVA DVWFDSGVAF FASLGQDWRK RWSELGPVDL
560 570 580 590 600
VLEGHDQLRG WFFSLLRTGV ILMDKAPYEA VLVHGFMLDE QGREMHKSSG
610 620 630 640 650
NYVEPSQVVS KYGRDTLRLW LLRNTTWEDA KFSWKSLDMT RRDLNIIWNV
660 670 680 690 700
YVFANTYMSL DEFKYSKYSY NDIKDYLKLE DIWLLSRYYR MLKEVIEAMK
710 720 730 740 750
EYKVHELANK VTAFIIDDIS RFYLRVTRKR AWNEANDPDK IAMYYVLYHV
760 770 780 790 800
LKGSLILLST VIPFTAEKIY LDFVQERLES ISMEKIPEIK EEFINSEIEE
810 820 830 840 850
AFEVAKEIID AGLNARAKAG IKLRWPLKEV YVFLVSDKDR RSIEKITDVL
860 870 880 890 900
SSLLNSKSII IEGIDGYKRF SKIRATPNTG SIGPTFKRLS VKVAEYIQNN
910 920 930 940 950
SDKVAQDIVS KGYHEFNVDS ENLRLDISHV NLVEEVEKGY VSARFSKGVV
960 970 980 990 1000
LLKQEMSKEE EEEGIIRDLI RRIQFMRKQL SLNVNEYILL SIRAPDDKVD
1010 1020 1030 1040 1050
LIKKWEQYIK NETRAKELRI GDVSGDLIQD WDVEEETYTI GVSKADVSVS
Length:1,050
Mass (Da):122,024
Last modified:August 30, 2005 - v2
Checksum:iA25145ED9D005935
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti164 – 1674KAYE → SIR in AAC41447. (PubMed:7708661)Curated
Sequence conflicti483 – 4831S → G in AAC41447. (PubMed:7708661)Curated
Sequence conflicti486 – 4861S → P in AAC41447. (PubMed:7708661)Curated
Sequence conflicti504 – 5129VRCEKCGGE → LDVKNVEGQ in AAC41447. (PubMed:7708661)Curated
Sequence conflicti614 – 63623RDTLR…FSWKS → QRYVKIMVTLEIPHGKMSKS HGN(PubMed:7708661)CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000077 Genomic DNA. Translation: AAY79991.1.
L37106 Genomic DNA. Translation: AAC41447.1.
RefSeqiWP_011277493.1. NC_007181.1.
YP_255284.1. NC_007181.1.

Genome annotation databases

EnsemblBacteriaiAAY79991; AAY79991; Saci_0599.
GeneIDi3473519.
KEGGisai:Saci_0599.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000077 Genomic DNA. Translation: AAY79991.1 .
L37106 Genomic DNA. Translation: AAC41447.1 .
RefSeqi WP_011277493.1. NC_007181.1.
YP_255284.1. NC_007181.1.

3D structure databases

ProteinModelPortali P46215.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 330779.Saci_0599.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAY79991 ; AAY79991 ; Saci_0599 .
GeneIDi 3473519.
KEGGi sai:Saci_0599.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi KWIISEI.

Enzyme and pathway databases

BioCyci SACI330779:GH9J-591-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome of Sulfolobus acidocaldarius, a model organism of the Crenarchaeota."
    Chen L., Bruegger K., Skovgaard M., Redder P., She Q., Torarinsson E., Greve B., Awayez M., Zibat A., Klenk H.-P., Garrett R.A.
    J. Bacteriol. 187:4992-4999(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770.
  2. "Root of the universal tree of life based on ancient aminoacyl-tRNA synthetase gene duplications."
    Brown J.R., Doolittle W.F.
    Proc. Natl. Acad. Sci. U.S.A. 92:2441-2445(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 84-636.

Entry informationi

Entry nameiSYI_SULAC
AccessioniPrimary (citable) accession number: P46215
Secondary accession number(s): Q4JB38
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: August 30, 2005
Last modified: November 26, 2014
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3