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P46215

- SYI_SULAC

UniProt

P46215 - SYI_SULAC

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 2 (30 Aug 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei597 – 5971ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciSACI330779:GH9J-591-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Saci_0599
    OrganismiSulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
    Taxonomic identifieri330779 [NCBI]
    Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
    ProteomesiUP000001018: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10501050Isoleucine--tRNA ligasePRO_0000098592Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi330779.Saci_0599.

    Structurei

    3D structure databases

    ProteinModelPortaliP46215.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi45 – 5612"HIGH" regionAdd
    BLAST
    Motifi594 – 5985"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiKWIISEI.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P46215-1 [UniParc]FASTAAdd to Basket

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    MTSKFDPLKF EEEVLKYWDD NNIYKKLKEI NEKNHKKFLF IDGPPYPSSP     50
    IPHIGTVWNK TIKDCILRYE RLMGYSVKDQ PGYDTHGLPI EVETEKRLGI 100
    KSKAEIIEKV GVDNFISKCK EFAVNNSKSL TQNFRNLGIF MDWENPYFTF 150
    NNDYISNSWA VIKKAYERGL LYKGVHVLHW CSRCETTLAD YEVSEYRDLE 200
    DPSIYVKFRV KGEPNRYLVI WTTTPWTLPA NVFVMINKDF EYADVRVGDE 250
    ILVIAKDRVK ELMKEARIKE YKILRVYKGE ELLGLEYEHP LADIVSAQSK 300
    INNHHKVLDG GEAVTLQEGT GLVHSAPGHG DVDFEIGKKY DMPVVMLVND 350
    KGEFTQDSGK YAGKYVRSAS EEIISDLKQR SALLHASKIV HRYPVCWRCK 400
    TPLILRAIEQ WFIAVSKLKD HLMGEIDRVR WIPDWGKTRI GNMVKEVRDW 450
    VISRQRFWGT PLPIWVCSNC QNIIVVGGVD ELSKISINQV PQDLHRPWID 500
    SVVVRCEKCG GEARRISDVA DVWFDSGVAF FASLGQDWRK RWSELGPVDL 550
    VLEGHDQLRG WFFSLLRTGV ILMDKAPYEA VLVHGFMLDE QGREMHKSSG 600
    NYVEPSQVVS KYGRDTLRLW LLRNTTWEDA KFSWKSLDMT RRDLNIIWNV 650
    YVFANTYMSL DEFKYSKYSY NDIKDYLKLE DIWLLSRYYR MLKEVIEAMK 700
    EYKVHELANK VTAFIIDDIS RFYLRVTRKR AWNEANDPDK IAMYYVLYHV 750
    LKGSLILLST VIPFTAEKIY LDFVQERLES ISMEKIPEIK EEFINSEIEE 800
    AFEVAKEIID AGLNARAKAG IKLRWPLKEV YVFLVSDKDR RSIEKITDVL 850
    SSLLNSKSII IEGIDGYKRF SKIRATPNTG SIGPTFKRLS VKVAEYIQNN 900
    SDKVAQDIVS KGYHEFNVDS ENLRLDISHV NLVEEVEKGY VSARFSKGVV 950
    LLKQEMSKEE EEEGIIRDLI RRIQFMRKQL SLNVNEYILL SIRAPDDKVD 1000
    LIKKWEQYIK NETRAKELRI GDVSGDLIQD WDVEEETYTI GVSKADVSVS 1050
    Length:1,050
    Mass (Da):122,024
    Last modified:August 30, 2005 - v2
    Checksum:iA25145ED9D005935
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti164 – 1674KAYE → SIR in AAC41447. (PubMed:7708661)Curated
    Sequence conflicti483 – 4831S → G in AAC41447. (PubMed:7708661)Curated
    Sequence conflicti486 – 4861S → P in AAC41447. (PubMed:7708661)Curated
    Sequence conflicti504 – 5129VRCEKCGGE → LDVKNVEGQ in AAC41447. (PubMed:7708661)Curated
    Sequence conflicti614 – 63623RDTLR…FSWKS → QRYVKIMVTLEIPHGKMSKS HGN(PubMed:7708661)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000077 Genomic DNA. Translation: AAY79991.1.
    L37106 Genomic DNA. Translation: AAC41447.1.
    RefSeqiWP_011277493.1. NC_007181.1.
    YP_255284.1. NC_007181.1.

    Genome annotation databases

    EnsemblBacteriaiAAY79991; AAY79991; Saci_0599.
    GeneIDi3473519.
    KEGGisai:Saci_0599.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000077 Genomic DNA. Translation: AAY79991.1 .
    L37106 Genomic DNA. Translation: AAC41447.1 .
    RefSeqi WP_011277493.1. NC_007181.1.
    YP_255284.1. NC_007181.1.

    3D structure databases

    ProteinModelPortali P46215.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 330779.Saci_0599.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAY79991 ; AAY79991 ; Saci_0599 .
    GeneIDi 3473519.
    KEGGi sai:Saci_0599.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi KWIISEI.

    Enzyme and pathway databases

    BioCyci SACI330779:GH9J-591-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of Sulfolobus acidocaldarius, a model organism of the Crenarchaeota."
      Chen L., Bruegger K., Skovgaard M., Redder P., She Q., Torarinsson E., Greve B., Awayez M., Zibat A., Klenk H.-P., Garrett R.A.
      J. Bacteriol. 187:4992-4999(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770.
    2. "Root of the universal tree of life based on ancient aminoacyl-tRNA synthetase gene duplications."
      Brown J.R., Doolittle W.F.
      Proc. Natl. Acad. Sci. U.S.A. 92:2441-2445(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 84-636.

    Entry informationi

    Entry nameiSYI_SULAC
    AccessioniPrimary (citable) accession number: P46215
    Secondary accession number(s): Q4JB38
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: August 30, 2005
    Last modified: October 1, 2014
    This is version 95 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3