Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei597ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Saci_0599
OrganismiSulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Taxonomic identifieri330779 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001018 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000985921 – 1050Isoleucine--tRNA ligaseAdd BLAST1050

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi330779.Saci_0599

Structurei

3D structure databases

ProteinModelPortaliP46215
SMRiP46215
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi45 – 56"HIGH" regionAdd BLAST12
Motifi594 – 598"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00807 Archaea
COG0060 LUCA
HOGENOMiHOG000246403
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG093Z0054

Family and domain databases

CDDicd07961 Anticodon_Ia_Ile_ABEc, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02003 Ile_tRNA_synth_type2, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033709 Anticodon_Ile_ABEc
IPR002301 Ile-tRNA-ligase
IPR023586 Ile-tRNA-ligase_type2
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 2 hits
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

P46215-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSKFDPLKF EEEVLKYWDD NNIYKKLKEI NEKNHKKFLF IDGPPYPSSP
60 70 80 90 100
IPHIGTVWNK TIKDCILRYE RLMGYSVKDQ PGYDTHGLPI EVETEKRLGI
110 120 130 140 150
KSKAEIIEKV GVDNFISKCK EFAVNNSKSL TQNFRNLGIF MDWENPYFTF
160 170 180 190 200
NNDYISNSWA VIKKAYERGL LYKGVHVLHW CSRCETTLAD YEVSEYRDLE
210 220 230 240 250
DPSIYVKFRV KGEPNRYLVI WTTTPWTLPA NVFVMINKDF EYADVRVGDE
260 270 280 290 300
ILVIAKDRVK ELMKEARIKE YKILRVYKGE ELLGLEYEHP LADIVSAQSK
310 320 330 340 350
INNHHKVLDG GEAVTLQEGT GLVHSAPGHG DVDFEIGKKY DMPVVMLVND
360 370 380 390 400
KGEFTQDSGK YAGKYVRSAS EEIISDLKQR SALLHASKIV HRYPVCWRCK
410 420 430 440 450
TPLILRAIEQ WFIAVSKLKD HLMGEIDRVR WIPDWGKTRI GNMVKEVRDW
460 470 480 490 500
VISRQRFWGT PLPIWVCSNC QNIIVVGGVD ELSKISINQV PQDLHRPWID
510 520 530 540 550
SVVVRCEKCG GEARRISDVA DVWFDSGVAF FASLGQDWRK RWSELGPVDL
560 570 580 590 600
VLEGHDQLRG WFFSLLRTGV ILMDKAPYEA VLVHGFMLDE QGREMHKSSG
610 620 630 640 650
NYVEPSQVVS KYGRDTLRLW LLRNTTWEDA KFSWKSLDMT RRDLNIIWNV
660 670 680 690 700
YVFANTYMSL DEFKYSKYSY NDIKDYLKLE DIWLLSRYYR MLKEVIEAMK
710 720 730 740 750
EYKVHELANK VTAFIIDDIS RFYLRVTRKR AWNEANDPDK IAMYYVLYHV
760 770 780 790 800
LKGSLILLST VIPFTAEKIY LDFVQERLES ISMEKIPEIK EEFINSEIEE
810 820 830 840 850
AFEVAKEIID AGLNARAKAG IKLRWPLKEV YVFLVSDKDR RSIEKITDVL
860 870 880 890 900
SSLLNSKSII IEGIDGYKRF SKIRATPNTG SIGPTFKRLS VKVAEYIQNN
910 920 930 940 950
SDKVAQDIVS KGYHEFNVDS ENLRLDISHV NLVEEVEKGY VSARFSKGVV
960 970 980 990 1000
LLKQEMSKEE EEEGIIRDLI RRIQFMRKQL SLNVNEYILL SIRAPDDKVD
1010 1020 1030 1040 1050
LIKKWEQYIK NETRAKELRI GDVSGDLIQD WDVEEETYTI GVSKADVSVS
Length:1,050
Mass (Da):122,024
Last modified:August 30, 2005 - v2
Checksum:iA25145ED9D005935
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti164 – 167KAYE → SIR in AAC41447 (PubMed:7708661).Curated4
Sequence conflicti483S → G in AAC41447 (PubMed:7708661).Curated1
Sequence conflicti486S → P in AAC41447 (PubMed:7708661).Curated1
Sequence conflicti504 – 512VRCEKCGGE → LDVKNVEGQ in AAC41447 (PubMed:7708661).Curated9
Sequence conflicti614 – 636RDTLR…FSWKS → QRYVKIMVTLEIPHGKMSKS HGN (PubMed:7708661).CuratedAdd BLAST23

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000077 Genomic DNA Translation: AAY79991.1
L37106 Genomic DNA Translation: AAC41447.1
RefSeqiWP_011277493.1, NC_007181.1

Genome annotation databases

EnsemblBacteriaiAAY79991; AAY79991; Saci_0599
GeneIDi3473519
KEGGisai:Saci_0599
PATRICifig|330779.12.peg.578

Similar proteinsi

Entry informationi

Entry nameiSYI_SULAC
AccessioniPrimary (citable) accession number: P46215
Secondary accession number(s): Q4JB38
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: August 30, 2005
Last modified: March 28, 2018
This is version 116 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health