SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P46215

- SYI_SULAC

UniProt

P46215 - SYI_SULAC

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Isoleucine--tRNA ligase
Gene
ileS, Saci_0599
Organism
Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei597 – 5971ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSACI330779:GH9J-591-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Saci_0599
OrganismiSulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Taxonomic identifieri330779 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
ProteomesiUP000001018: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10501050Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098592Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi330779.Saci_0599.

Structurei

3D structure databases

ProteinModelPortaliP46215.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi45 – 5612"HIGH" regionUniRule annotation
Add
BLAST
Motifi594 – 5985"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiKWIISEI.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P46215-1 [UniParc]FASTAAdd to Basket

« Hide

MTSKFDPLKF EEEVLKYWDD NNIYKKLKEI NEKNHKKFLF IDGPPYPSSP     50
IPHIGTVWNK TIKDCILRYE RLMGYSVKDQ PGYDTHGLPI EVETEKRLGI 100
KSKAEIIEKV GVDNFISKCK EFAVNNSKSL TQNFRNLGIF MDWENPYFTF 150
NNDYISNSWA VIKKAYERGL LYKGVHVLHW CSRCETTLAD YEVSEYRDLE 200
DPSIYVKFRV KGEPNRYLVI WTTTPWTLPA NVFVMINKDF EYADVRVGDE 250
ILVIAKDRVK ELMKEARIKE YKILRVYKGE ELLGLEYEHP LADIVSAQSK 300
INNHHKVLDG GEAVTLQEGT GLVHSAPGHG DVDFEIGKKY DMPVVMLVND 350
KGEFTQDSGK YAGKYVRSAS EEIISDLKQR SALLHASKIV HRYPVCWRCK 400
TPLILRAIEQ WFIAVSKLKD HLMGEIDRVR WIPDWGKTRI GNMVKEVRDW 450
VISRQRFWGT PLPIWVCSNC QNIIVVGGVD ELSKISINQV PQDLHRPWID 500
SVVVRCEKCG GEARRISDVA DVWFDSGVAF FASLGQDWRK RWSELGPVDL 550
VLEGHDQLRG WFFSLLRTGV ILMDKAPYEA VLVHGFMLDE QGREMHKSSG 600
NYVEPSQVVS KYGRDTLRLW LLRNTTWEDA KFSWKSLDMT RRDLNIIWNV 650
YVFANTYMSL DEFKYSKYSY NDIKDYLKLE DIWLLSRYYR MLKEVIEAMK 700
EYKVHELANK VTAFIIDDIS RFYLRVTRKR AWNEANDPDK IAMYYVLYHV 750
LKGSLILLST VIPFTAEKIY LDFVQERLES ISMEKIPEIK EEFINSEIEE 800
AFEVAKEIID AGLNARAKAG IKLRWPLKEV YVFLVSDKDR RSIEKITDVL 850
SSLLNSKSII IEGIDGYKRF SKIRATPNTG SIGPTFKRLS VKVAEYIQNN 900
SDKVAQDIVS KGYHEFNVDS ENLRLDISHV NLVEEVEKGY VSARFSKGVV 950
LLKQEMSKEE EEEGIIRDLI RRIQFMRKQL SLNVNEYILL SIRAPDDKVD 1000
LIKKWEQYIK NETRAKELRI GDVSGDLIQD WDVEEETYTI GVSKADVSVS 1050
Length:1,050
Mass (Da):122,024
Last modified:August 30, 2005 - v2
Checksum:iA25145ED9D005935
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti164 – 1674KAYE → SIR in AAC41447. 1 Publication
Sequence conflicti483 – 4831S → G in AAC41447. 1 Publication
Sequence conflicti486 – 4861S → P in AAC41447. 1 Publication
Sequence conflicti504 – 5129VRCEKCGGE → LDVKNVEGQ in AAC41447. 1 Publication
Sequence conflicti614 – 63623RDTLR…FSWKS → QRYVKIMVTLEIPHGKMSKS HGN1 Publication
Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000077 Genomic DNA. Translation: AAY79991.1.
L37106 Genomic DNA. Translation: AAC41447.1.
RefSeqiWP_011277493.1. NC_007181.1.
YP_255284.1. NC_007181.1.

Genome annotation databases

EnsemblBacteriaiAAY79991; AAY79991; Saci_0599.
GeneIDi3473519.
KEGGisai:Saci_0599.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000077 Genomic DNA. Translation: AAY79991.1 .
L37106 Genomic DNA. Translation: AAC41447.1 .
RefSeqi WP_011277493.1. NC_007181.1.
YP_255284.1. NC_007181.1.

3D structure databases

ProteinModelPortali P46215.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 330779.Saci_0599.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAY79991 ; AAY79991 ; Saci_0599 .
GeneIDi 3473519.
KEGGi sai:Saci_0599.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi KWIISEI.

Enzyme and pathway databases

BioCyci SACI330779:GH9J-591-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome of Sulfolobus acidocaldarius, a model organism of the Crenarchaeota."
    Chen L., Bruegger K., Skovgaard M., Redder P., She Q., Torarinsson E., Greve B., Awayez M., Zibat A., Klenk H.-P., Garrett R.A.
    J. Bacteriol. 187:4992-4999(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770.
  2. "Root of the universal tree of life based on ancient aminoacyl-tRNA synthetase gene duplications."
    Brown J.R., Doolittle W.F.
    Proc. Natl. Acad. Sci. U.S.A. 92:2441-2445(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 84-636.

Entry informationi

Entry nameiSYI_SULAC
AccessioniPrimary (citable) accession number: P46215
Secondary accession number(s): Q4JB38
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: August 30, 2005
Last modified: September 3, 2014
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi