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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei628ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:PF1096
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000001013 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000985891 – 1066Isoleucine--tRNA ligaseAdd BLAST1066

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi186497.PF1096.

Structurei

3D structure databases

ProteinModelPortaliP46214.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi49 – 59"HIGH" regionAdd BLAST11
Motifi625 – 629"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00807. Archaea.
COG0060. LUCA.
HOGENOMiHOG000246403.
KOiK01870.
OMAiGFGRAPY.

Family and domain databases

CDDicd07961. Anticodon_Ia_Ile_ABEc. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033709. Anticodon_Ile_ABEc.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P46214-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKEPEFRDY TPGKLEEKIE QFWKESNIYQ KVKELRKNGP KYYFLDGPPY
60 70 80 90 100
VSGAIHLGTA WNKIIKDMII RFRTMQGYNV WRQPGYDMHG LPIEVKVEQA
110 120 130 140 150
LGLKTKKEIE EKIGVENFIQ KCKEFALNNL RIMTEQFKML GVWMDWDNPY
160 170 180 190 200
MTIKNEYIES AWFTLKRAWE KGLLEKDKRV LHWCPRCETA LAEHEVRGEY
210 220 230 240 250
KLRKDPSIYV KFPVEGKENE YLLIWTTTPW TLPANLAVSA HPDYDYVKVR
260 270 280 290 300
VDLNGREEYW ILAKALVEKV LGDIGVKGEV VEEFKGKELE GLRYVHILMD
310 320 330 340 350
EYPRQKEFRE KYEWVHRVIL ADFVTLEEGT GLVHTAPGHG EEDFEVGQKY
360 370 380 390 400
GLPVYSPVDD QGKYVEGKWK GVYVKEADPQ IIEHLKEKGY LVKAGEIEHK
410 420 430 440 450
YPHCWRCKTP LIFRATDQWF LKVSKVKEKI IKENDEKVTW YPEWVKIRFD
460 470 480 490 500
NGVRDSGDWV ISRQRYWGIP LPIWQSEDGE IYVVGSWKEL VELAVAIEVN
510 520 530 540 550
GERIDLPESY EEKLKVIEEK LGPEDLHRPY VDAFIIKVNG KEMRRVKDVV
560 570 580 590 600
DVWFDSGIAS WASLGYPRNK ELFEKLWPAD FIVEGEDQVT KWFYSQQAAS
610 620 630 640 650
VIAFDTVPYR AVAMHGYVLD EKGDKMSKSL GNIIRPEEVV EKAGRDTFRF
660 670 680 690 700
YMLWATNPWE NLKFSWKGVE QVRRMLNILW NVYVLSATYM SLDNFDPRNV
710 720 730 740 750
KVEELEFREE DKWILSRVNN LIKEVENGIE TFYLTKATRA LYNFVVEDLS
760 770 780 790 800
RWYVRLIRKR LWVEGDDPDK LAAYYTLWKV FDVLLRLMAP FTPYITEEIY
810 820 830 840 850
QNIMRPFTGI ESVHMLDWPK PDESAVDEEL EREMEFIRRI VEAGSAARQK
860 870 880 890 900
AKIKLRYPVR KIIIETQDDT VKKAVERLNY ILRDQLNAKE VVVGNVEREI
910 920 930 940 950
TVKPNFAKVG PEFKGDARLV AKWISEHGLE LYEKGEVDVE IEGKKFHLTR
960 970 980 990 1000
EHIIVEENIP DFLVAEDFEG GRVYVDKTLT RELLAEGLAR EFVRRIQEMR
1010 1020 1030 1040 1050
KRLDLDVNDR IVVTIETTEE NRELLQENLE YIMRETRAIE VRFEEAKGYV
1060
VEWPEVQAKI GIEKIE
Length:1,066
Mass (Da):125,752
Last modified:September 26, 2001 - v2
Checksum:i4704936EBC5D2272
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti88M → T in AAC41446 (PubMed:7708661).Curated1
Sequence conflicti119I → V in AAC41446 (PubMed:7708661).Curated1
Sequence conflicti167R → W in AAC41446 (PubMed:7708661).Curated1
Sequence conflicti241H → L in AAC41446 (PubMed:7708661).Curated1
Sequence conflicti250R → G in AAC41446 (PubMed:7708661).Curated1
Sequence conflicti494A → T in AAC41446 (PubMed:7708661).Curated1
Sequence conflicti501G → S in AAC41446 (PubMed:7708661).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL81220.1.
L37105 Genomic DNA. Translation: AAC41446.1.
RefSeqiWP_011012235.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL81220; AAL81220; PF1096.
GeneIDi1468964.
KEGGipfu:PF1096.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009950 Genomic DNA. Translation: AAL81220.1.
L37105 Genomic DNA. Translation: AAC41446.1.
RefSeqiWP_011012235.1. NC_003413.1.

3D structure databases

ProteinModelPortaliP46214.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi186497.PF1096.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL81220; AAL81220; PF1096.
GeneIDi1468964.
KEGGipfu:PF1096.

Phylogenomic databases

eggNOGiarCOG00807. Archaea.
COG0060. LUCA.
HOGENOMiHOG000246403.
KOiK01870.
OMAiGFGRAPY.

Family and domain databases

CDDicd07961. Anticodon_Ia_Ile_ABEc. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033709. Anticodon_Ile_ABEc.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYI_PYRFU
AccessioniPrimary (citable) accession number: P46214
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: September 26, 2001
Last modified: October 5, 2016
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.