Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P46214

- SYI_PYRFU

UniProt

P46214 - SYI_PYRFU

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 2 (26 Sep 2001)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei628 – 6281ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:PF1096
    OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
    Taxonomic identifieri186497 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
    ProteomesiUP000001013: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10661066Isoleucine--tRNA ligasePRO_0000098589Add
    BLAST

    Proteomic databases

    PRIDEiP46214.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi186497.PF1096.

    Structurei

    3D structure databases

    ProteinModelPortaliP46214.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi49 – 5911"HIGH" regionAdd
    BLAST
    Motifi625 – 6295"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiMNIRDAN.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P46214-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIKEPEFRDY TPGKLEEKIE QFWKESNIYQ KVKELRKNGP KYYFLDGPPY     50
    VSGAIHLGTA WNKIIKDMII RFRTMQGYNV WRQPGYDMHG LPIEVKVEQA 100
    LGLKTKKEIE EKIGVENFIQ KCKEFALNNL RIMTEQFKML GVWMDWDNPY 150
    MTIKNEYIES AWFTLKRAWE KGLLEKDKRV LHWCPRCETA LAEHEVRGEY 200
    KLRKDPSIYV KFPVEGKENE YLLIWTTTPW TLPANLAVSA HPDYDYVKVR 250
    VDLNGREEYW ILAKALVEKV LGDIGVKGEV VEEFKGKELE GLRYVHILMD 300
    EYPRQKEFRE KYEWVHRVIL ADFVTLEEGT GLVHTAPGHG EEDFEVGQKY 350
    GLPVYSPVDD QGKYVEGKWK GVYVKEADPQ IIEHLKEKGY LVKAGEIEHK 400
    YPHCWRCKTP LIFRATDQWF LKVSKVKEKI IKENDEKVTW YPEWVKIRFD 450
    NGVRDSGDWV ISRQRYWGIP LPIWQSEDGE IYVVGSWKEL VELAVAIEVN 500
    GERIDLPESY EEKLKVIEEK LGPEDLHRPY VDAFIIKVNG KEMRRVKDVV 550
    DVWFDSGIAS WASLGYPRNK ELFEKLWPAD FIVEGEDQVT KWFYSQQAAS 600
    VIAFDTVPYR AVAMHGYVLD EKGDKMSKSL GNIIRPEEVV EKAGRDTFRF 650
    YMLWATNPWE NLKFSWKGVE QVRRMLNILW NVYVLSATYM SLDNFDPRNV 700
    KVEELEFREE DKWILSRVNN LIKEVENGIE TFYLTKATRA LYNFVVEDLS 750
    RWYVRLIRKR LWVEGDDPDK LAAYYTLWKV FDVLLRLMAP FTPYITEEIY 800
    QNIMRPFTGI ESVHMLDWPK PDESAVDEEL EREMEFIRRI VEAGSAARQK 850
    AKIKLRYPVR KIIIETQDDT VKKAVERLNY ILRDQLNAKE VVVGNVEREI 900
    TVKPNFAKVG PEFKGDARLV AKWISEHGLE LYEKGEVDVE IEGKKFHLTR 950
    EHIIVEENIP DFLVAEDFEG GRVYVDKTLT RELLAEGLAR EFVRRIQEMR 1000
    KRLDLDVNDR IVVTIETTEE NRELLQENLE YIMRETRAIE VRFEEAKGYV 1050
    VEWPEVQAKI GIEKIE 1066
    Length:1,066
    Mass (Da):125,752
    Last modified:September 26, 2001 - v2
    Checksum:i4704936EBC5D2272
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti88 – 881M → T in AAC41446. (PubMed:7708661)Curated
    Sequence conflicti119 – 1191I → V in AAC41446. (PubMed:7708661)Curated
    Sequence conflicti167 – 1671R → W in AAC41446. (PubMed:7708661)Curated
    Sequence conflicti241 – 2411H → L in AAC41446. (PubMed:7708661)Curated
    Sequence conflicti250 – 2501R → G in AAC41446. (PubMed:7708661)Curated
    Sequence conflicti494 – 4941A → T in AAC41446. (PubMed:7708661)Curated
    Sequence conflicti501 – 5011G → S in AAC41446. (PubMed:7708661)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE009950 Genomic DNA. Translation: AAL81220.1.
    L37105 Genomic DNA. Translation: AAC41446.1.
    RefSeqiNP_578825.1. NC_003413.1.
    WP_011012235.1. NC_003413.1.

    Genome annotation databases

    EnsemblBacteriaiAAL81220; AAL81220; PF1096.
    GeneIDi1468964.
    KEGGipfu:PF1096.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE009950 Genomic DNA. Translation: AAL81220.1 .
    L37105 Genomic DNA. Translation: AAC41446.1 .
    RefSeqi NP_578825.1. NC_003413.1.
    WP_011012235.1. NC_003413.1.

    3D structure databases

    ProteinModelPortali P46214.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 186497.PF1096.

    Proteomic databases

    PRIDEi P46214.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL81220 ; AAL81220 ; PF1096 .
    GeneIDi 1468964.
    KEGGi pfu:PF1096.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi MNIRDAN.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
      Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
      Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
    2. "Root of the universal tree of life based on ancient aminoacyl-tRNA synthetase gene duplications."
      Brown J.R., Doolittle W.F.
      Proc. Natl. Acad. Sci. U.S.A. 92:2441-2445(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 87-632.

    Entry informationi

    Entry nameiSYI_PYRFU
    AccessioniPrimary (citable) accession number: P46214
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: September 26, 2001
    Last modified: October 1, 2014
    This is version 96 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3