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P46214

- SYI_PYRFU

UniProt

P46214 - SYI_PYRFU

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei628 – 6281ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:PF1096
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000001013: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10661066Isoleucine--tRNA ligasePRO_0000098589Add
BLAST

Proteomic databases

PRIDEiP46214.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi186497.PF1096.

Structurei

3D structure databases

ProteinModelPortaliP46214.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi49 – 5911"HIGH" regionAdd
BLAST
Motifi625 – 6295"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiMNIRDAN.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P46214-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIKEPEFRDY TPGKLEEKIE QFWKESNIYQ KVKELRKNGP KYYFLDGPPY
60 70 80 90 100
VSGAIHLGTA WNKIIKDMII RFRTMQGYNV WRQPGYDMHG LPIEVKVEQA
110 120 130 140 150
LGLKTKKEIE EKIGVENFIQ KCKEFALNNL RIMTEQFKML GVWMDWDNPY
160 170 180 190 200
MTIKNEYIES AWFTLKRAWE KGLLEKDKRV LHWCPRCETA LAEHEVRGEY
210 220 230 240 250
KLRKDPSIYV KFPVEGKENE YLLIWTTTPW TLPANLAVSA HPDYDYVKVR
260 270 280 290 300
VDLNGREEYW ILAKALVEKV LGDIGVKGEV VEEFKGKELE GLRYVHILMD
310 320 330 340 350
EYPRQKEFRE KYEWVHRVIL ADFVTLEEGT GLVHTAPGHG EEDFEVGQKY
360 370 380 390 400
GLPVYSPVDD QGKYVEGKWK GVYVKEADPQ IIEHLKEKGY LVKAGEIEHK
410 420 430 440 450
YPHCWRCKTP LIFRATDQWF LKVSKVKEKI IKENDEKVTW YPEWVKIRFD
460 470 480 490 500
NGVRDSGDWV ISRQRYWGIP LPIWQSEDGE IYVVGSWKEL VELAVAIEVN
510 520 530 540 550
GERIDLPESY EEKLKVIEEK LGPEDLHRPY VDAFIIKVNG KEMRRVKDVV
560 570 580 590 600
DVWFDSGIAS WASLGYPRNK ELFEKLWPAD FIVEGEDQVT KWFYSQQAAS
610 620 630 640 650
VIAFDTVPYR AVAMHGYVLD EKGDKMSKSL GNIIRPEEVV EKAGRDTFRF
660 670 680 690 700
YMLWATNPWE NLKFSWKGVE QVRRMLNILW NVYVLSATYM SLDNFDPRNV
710 720 730 740 750
KVEELEFREE DKWILSRVNN LIKEVENGIE TFYLTKATRA LYNFVVEDLS
760 770 780 790 800
RWYVRLIRKR LWVEGDDPDK LAAYYTLWKV FDVLLRLMAP FTPYITEEIY
810 820 830 840 850
QNIMRPFTGI ESVHMLDWPK PDESAVDEEL EREMEFIRRI VEAGSAARQK
860 870 880 890 900
AKIKLRYPVR KIIIETQDDT VKKAVERLNY ILRDQLNAKE VVVGNVEREI
910 920 930 940 950
TVKPNFAKVG PEFKGDARLV AKWISEHGLE LYEKGEVDVE IEGKKFHLTR
960 970 980 990 1000
EHIIVEENIP DFLVAEDFEG GRVYVDKTLT RELLAEGLAR EFVRRIQEMR
1010 1020 1030 1040 1050
KRLDLDVNDR IVVTIETTEE NRELLQENLE YIMRETRAIE VRFEEAKGYV
1060
VEWPEVQAKI GIEKIE
Length:1,066
Mass (Da):125,752
Last modified:September 26, 2001 - v2
Checksum:i4704936EBC5D2272
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti88 – 881M → T in AAC41446. (PubMed:7708661)Curated
Sequence conflicti119 – 1191I → V in AAC41446. (PubMed:7708661)Curated
Sequence conflicti167 – 1671R → W in AAC41446. (PubMed:7708661)Curated
Sequence conflicti241 – 2411H → L in AAC41446. (PubMed:7708661)Curated
Sequence conflicti250 – 2501R → G in AAC41446. (PubMed:7708661)Curated
Sequence conflicti494 – 4941A → T in AAC41446. (PubMed:7708661)Curated
Sequence conflicti501 – 5011G → S in AAC41446. (PubMed:7708661)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE009950 Genomic DNA. Translation: AAL81220.1.
L37105 Genomic DNA. Translation: AAC41446.1.
RefSeqiNP_578825.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL81220; AAL81220; PF1096.
GeneIDi1468964.
KEGGipfu:PF1096.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE009950 Genomic DNA. Translation: AAL81220.1 .
L37105 Genomic DNA. Translation: AAC41446.1 .
RefSeqi NP_578825.1. NC_003413.1.

3D structure databases

ProteinModelPortali P46214.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 186497.PF1096.

Proteomic databases

PRIDEi P46214.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL81220 ; AAL81220 ; PF1096 .
GeneIDi 1468964.
KEGGi pfu:PF1096.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi MNIRDAN.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
    Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
    Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
  2. "Root of the universal tree of life based on ancient aminoacyl-tRNA synthetase gene duplications."
    Brown J.R., Doolittle W.F.
    Proc. Natl. Acad. Sci. U.S.A. 92:2441-2445(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 87-632.

Entry informationi

Entry nameiSYI_PYRFU
AccessioniPrimary (citable) accession number: P46214
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: September 26, 2001
Last modified: October 29, 2014
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3