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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei553Aminoacyl-adenylateUniRule annotation1
Binding sitei597ATPUniRule annotation1
Metal bindingi887ZincUniRule annotation1
Metal bindingi890ZincUniRule annotation1
Metal bindingi907ZincUniRule annotation1
Metal bindingi910ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:TM_1361
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000984941 – 919Isoleucine--tRNA ligaseAdd BLAST919

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi243274.TM1361

Structurei

3D structure databases

ProteinModelPortaliP46213
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi57 – 67"HIGH" regionAdd BLAST11
Motifi594 – 598"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
InParanoidiP46213
KOiK01870
OMAiHLGTAWN

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

P46213-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEYKNTLNLP KTSFPMKANL VNKEKVFLEE WEKMDLYNYV LEQRKGKPLF
60 70 80 90 100
VLHDGPPYAN GHIHIGTALN KILKDIVVKY KTMRGYRAPY VPGWDTHGLP
110 120 130 140 150
IEHRVSQELG EKIKEMSPAE IRKKCEEFAL RFVDIQREEF KRLGVRGDWE
160 170 180 190 200
NPYITLKPDY EVKILDVFKT LVEQGNVYRS LKPIYWCPRC RTALAEAEIE
210 220 230 240 250
YHDHKSPSIY VKFRSKEDPN FFIVIWTTTP WTLPANVGIA LHPDYEYSVV
260 270 280 290 300
KVGEEKWVIA TDLLDAFSKE TGIDCSNVVE KIKGKDLEGK EFVHPIFDDR
310 320 330 340 350
TSRVILADYV SLETGTGCVH IAPGHGEEDY IYGHVQYGLP IVSPVDEEGR
360 370 380 390 400
FTEEAGKYKG MFIEDANEVI IEDLKRKGIL VHASSITHSY PHCWRCKGPV
410 420 430 440 450
IFRATEQWFI SVDHNNLRQK VLEEIDKVKW IPEWGRNRIR SMVEERPDWC
460 470 480 490 500
ISRQRVWGTP IPAVKCKECG EVVLDPKVIE HFMKIVEKEG TNAWFEKEVE
510 520 530 540 550
ELIPEDFVCP KCGKRSFEKM LDTLDVWIDS GASFEYITTK REDHPFPLDM
560 570 580 590 600
YLEGSDQHRG WFHSSIFLAV AKRGSAPYKE VLTHGFIKDE QGRKMSKSLG
610 620 630 640 650
NVVDPMEVVE KYGAEILRLW LASSDYFNDI KISMRIVEQQ TEVYRKIRNT
660 670 680 690 700
FRFLLGNLED FDPELDRVPH EKLLTIDRWA LGRLQEIIKR ATEYYDSYEF
710 720 730 740 750
SKVYNLVVKY CTTELSSLYL DVVKDRLYVE AKDSIYRRSA QTVMHEILIS
760 770 780 790 800
LMKILAPIMT FTMEEVYSHL HEKDRKYKTV QAEYWPEYRE EFIDRKLMED
810 820 830 840 850
FEKLLSIRED VLKALEEKRQ QDVIGHSLDA EVVLVPKNDT IKALLEKYRD
860 870 880 890 900
ILEELFIVSK VSLSDASGEL KGELVEVTVK HAEGEKCQRC WKYTTEISAS
910
EDFPGVCPRC LAVLKGERK
Length:919
Mass (Da):107,156
Last modified:May 30, 2000 - v2
Checksum:i40E4D0876010C385
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA Translation: AAD36431.1
L37104 Genomic DNA Translation: AAC41448.1
PIRiB72263
RefSeqiNP_229162.1, NC_000853.1
WP_004081557.1, NZ_CP011107.1

Genome annotation databases

EnsemblBacteriaiAAD36431; AAD36431; TM_1361
GeneIDi898119
KEGGitma:TM1361

Similar proteinsi

Entry informationi

Entry nameiSYI_THEMA
AccessioniPrimary (citable) accession number: P46213
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: May 30, 2000
Last modified: March 28, 2018
This is version 138 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health