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Protein

Translation initiation factor IF-2, mitochondrial

Gene

MTIF2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi187 – 1948GTPBy similarity
Nucleotide bindingi234 – 2374GTPBy similarity
Nucleotide bindingi288 – 2914GTPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-5368286. Mitochondrial translation initiation.

Names & Taxonomyi

Protein namesi
Recommended name:
Translation initiation factor IF-2, mitochondrial
Short name:
IF-2(Mt)
Short name:
IF-2Mt
Short name:
IF2(mt)
Gene namesi
Name:MTIF2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:7441. MTIF2.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: BHF-UCL
  • nucleoplasm Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA31243.

Polymorphism and mutation databases

BioMutaiMTIF2.
DMDMi223590069.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2929MitochondrionSequence analysisAdd
BLAST
Chaini30 – 727698Translation initiation factor IF-2, mitochondrialPRO_0000014480Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei688 – 6881PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP46199.
MaxQBiP46199.
PaxDbiP46199.
PeptideAtlasiP46199.
PRIDEiP46199.

PTM databases

iPTMnetiP46199.
PhosphoSiteiP46199.

Expressioni

Tissue specificityi

Expressed in all tissues examined. Highest level in skeletal muscle.

Gene expression databases

BgeeiP46199.
CleanExiHS_MTIF2.
ExpressionAtlasiP46199. baseline and differential.
GenevisibleiP46199. HS.

Organism-specific databases

HPAiHPA006021.
HPA061422.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi110625. 36 interactions.
DIPiDIP-27596N.
IntActiP46199. 5 interactions.
MINTiMINT-88709.
STRINGi9606.ENSP00000263629.

Structurei

3D structure databases

ProteinModelPortaliP46199.
SMRiP46199. Positions 178-713.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini178 – 348171tr-type GAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni187 – 1948G1By similarity
Regioni212 – 2165G2By similarity
Regioni234 – 2374G3By similarity
Regioni288 – 2914G4By similarity
Regioni324 – 3263G5By similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1145. Eukaryota.
COG0532. LUCA.
GeneTreeiENSGT00550000074932.
HOGENOMiHOG000076906.
HOVERGENiHBG006124.
InParanoidiP46199.
KOiK02519.
OMAiKVVEVWI.
OrthoDBiEOG776SPH.
PhylomeDBiP46199.
TreeFamiTF105682.

Family and domain databases

Gene3Di3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00100_B. IF_2_B.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR000178. TF_IF2_bacterial-like.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
[Graphical view]
PfamiPF11987. IF-2. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 2 hits.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51722. G_TR_2. 1 hit.
PS01176. IF2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P46199-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQKLLKLEN LLRFHTIYRQ LHSLCQRRAL RQWRHGFSSA YPVWTAQLCA
60 70 80 90 100
WPWPTDVLTG AALSQYRLLV TKKEEGPWKS QLSSTKSKKV VEVWIGMTIE
110 120 130 140 150
ELARAMEKNT DYVYEALLNT DIDIDSLEAD SHLDEVWIKE VITKAGMKLK
160 170 180 190 200
WSKLKQDKVR KNKDAVRRPQ ADPALLTPRS PVVTIMGHVD HGKTTLLDKF
210 220 230 240 250
RKTQVAAVET GGITQHIGAF LVSLPSGEKI TFLDTPGHAA FSAMRARGAQ
260 270 280 290 300
VTDIVVLVVA ADDGVMKQTV ESIQHAKDAQ VPIILAVNKC DKAEADPEKV
310 320 330 340 350
KKELLAYDVV CEDYGGDVQA VPVSALTGDN LMALAEATVA LAEMLELKAD
360 370 380 390 400
PNGPVEGTVI ESFTDKGRGL VTTAIIQRGT LRKGSVLVAG KCWAKVRLMF
410 420 430 440 450
DENGKTIDEA YPSMPVGITG WRDLPSAGEE ILEVESEPRA REVVDWRKYE
460 470 480 490 500
QEQEKGQEDL KIIEEKRKEH KEAHQKAREK YGHLLWKKRS ILRFLERKEQ
510 520 530 540 550
IPLKPKEKRE RDSNVLSVII KGDVDGSVEA ILNIIDTYDA SHECELELVH
560 570 580 590 600
FGVGDVSAND VNLAETFDGV IYGFNVNAGN VIQQSAAKKG VKIKLHKIIY
610 620 630 640 650
RLVEDLQEEL SSRLPCAVEE HPVGEASILA TFSVTEGKKK VPVAGCRVQK
660 670 680 690 700
GQLEKQKKFK LTRNGHVIWK GSLTSLKHHK DDISIVKTGM DCGLSLDEDN
710 720
MEFQVGDRIV CYEEKQIQAK TSWDPGF
Length:727
Mass (Da):81,317
Last modified:February 10, 2009 - v2
Checksum:iAD2951AF3A5EB9E6
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti59 – 591T → N.2 Publications
Corresponds to variant rs1056445 [ dbSNP | Ensembl ].
VAR_054428
Natural varianti556 – 5561V → I.2 Publications
Corresponds to variant rs11357 [ dbSNP | Ensembl ].
VAR_014883

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L34600 mRNA. Translation: AAA67038.1.
AF494407 mRNA. Translation: AAM14617.1.
AF495546
, AF495543, AF495544, AF495545 Genomic DNA. Translation: AAM70196.1.
AC012358 Genomic DNA. No translation available.
CH471053 Genomic DNA. Translation: EAX00104.1.
CH471053 Genomic DNA. Translation: EAX00105.1.
CCDSiCCDS1853.1.
PIRiA55628.
RefSeqiNP_001005369.1. NM_001005369.1.
NP_001307930.1. NM_001321001.1.
NP_001307931.1. NM_001321002.1.
NP_001307932.1. NM_001321003.1.
NP_001307933.1. NM_001321004.1.
NP_001307934.1. NM_001321005.1.
NP_002444.2. NM_002453.2.
XP_005264392.1. XM_005264335.2.
XP_011531171.1. XM_011532869.1.
XP_011531173.1. XM_011532871.1.
UniGeneiHs.149894.

Genome annotation databases

EnsembliENST00000263629; ENSP00000263629; ENSG00000085760.
ENST00000394600; ENSP00000378099; ENSG00000085760.
ENST00000403721; ENSP00000384481; ENSG00000085760.
GeneIDi4528.
KEGGihsa:4528.
UCSCiuc002ryo.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L34600 mRNA. Translation: AAA67038.1.
AF494407 mRNA. Translation: AAM14617.1.
AF495546
, AF495543, AF495544, AF495545 Genomic DNA. Translation: AAM70196.1.
AC012358 Genomic DNA. No translation available.
CH471053 Genomic DNA. Translation: EAX00104.1.
CH471053 Genomic DNA. Translation: EAX00105.1.
CCDSiCCDS1853.1.
PIRiA55628.
RefSeqiNP_001005369.1. NM_001005369.1.
NP_001307930.1. NM_001321001.1.
NP_001307931.1. NM_001321002.1.
NP_001307932.1. NM_001321003.1.
NP_001307933.1. NM_001321004.1.
NP_001307934.1. NM_001321005.1.
NP_002444.2. NM_002453.2.
XP_005264392.1. XM_005264335.2.
XP_011531171.1. XM_011532869.1.
XP_011531173.1. XM_011532871.1.
UniGeneiHs.149894.

3D structure databases

ProteinModelPortaliP46199.
SMRiP46199. Positions 178-713.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110625. 36 interactions.
DIPiDIP-27596N.
IntActiP46199. 5 interactions.
MINTiMINT-88709.
STRINGi9606.ENSP00000263629.

PTM databases

iPTMnetiP46199.
PhosphoSiteiP46199.

Polymorphism and mutation databases

BioMutaiMTIF2.
DMDMi223590069.

Proteomic databases

EPDiP46199.
MaxQBiP46199.
PaxDbiP46199.
PeptideAtlasiP46199.
PRIDEiP46199.

Protocols and materials databases

DNASUi4528.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000263629; ENSP00000263629; ENSG00000085760.
ENST00000394600; ENSP00000378099; ENSG00000085760.
ENST00000403721; ENSP00000384481; ENSG00000085760.
GeneIDi4528.
KEGGihsa:4528.
UCSCiuc002ryo.3. human.

Organism-specific databases

CTDi4528.
GeneCardsiMTIF2.
H-InvDBHIX0200341.
HGNCiHGNC:7441. MTIF2.
HPAiHPA006021.
HPA061422.
MIMi603766. gene.
neXtProtiNX_P46199.
PharmGKBiPA31243.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1145. Eukaryota.
COG0532. LUCA.
GeneTreeiENSGT00550000074932.
HOGENOMiHOG000076906.
HOVERGENiHBG006124.
InParanoidiP46199.
KOiK02519.
OMAiKVVEVWI.
OrthoDBiEOG776SPH.
PhylomeDBiP46199.
TreeFamiTF105682.

Enzyme and pathway databases

ReactomeiR-HSA-5368286. Mitochondrial translation initiation.

Miscellaneous databases

ChiTaRSiMTIF2. human.
GeneWikiiMTIF2.
GenomeRNAii4528.
PROiP46199.
SOURCEiSearch...

Gene expression databases

BgeeiP46199.
CleanExiHS_MTIF2.
ExpressionAtlasiP46199. baseline and differential.
GenevisibleiP46199. HS.

Family and domain databases

Gene3Di3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00100_B. IF_2_B.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR000178. TF_IF2_bacterial-like.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
[Graphical view]
PfamiPF11987. IF-2. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 2 hits.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51722. G_TR_2. 1 hit.
PS01176. IF2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequence analysis of the human mitochondrial translational initiation factor 2 cDNA."
    Ma L., Spremulli L.L.
    J. Biol. Chem. 270:1859-1865(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS ASN-59 AND ILE-556.
    Tissue: Liver.
  2. "The human mitochondrial translation initiation factor 2 gene (MTIF2): transcriptional analysis and identification of a pseudogene."
    Overman R.G. Jr., Enderle P.J., Farrow J.M. III, Wiley J.E., Farwell M.A.
    Biochim. Biophys. Acta 1628:195-205(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], VARIANTS ASN-59 AND ILE-556.
    Tissue: Heart.
  3. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiIF2M_HUMAN
AccessioniPrimary (citable) accession number: P46199
Secondary accession number(s): D6W5D0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: February 10, 2009
Last modified: July 6, 2016
This is version 156 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.