Reviewed, UniProtKB/Swiss-Prot P46196 (MK01_BOVIN)

Names and origin

Protein names

Recommended name:
    Mitogen-activated protein kinase 1
    EC=2.7.11.24
Alternative name(s):
    Extracellular signal-regulated kinase 2
      Short name=ERK-2
    Mitogen-activated protein kinase 2
      Short name=MAP kinase 2
      Short name=MAPK 2
    p42-MAPK
    ERT1

Gene names

Name:	MAPK1
Synonyms:	ERK2, PRKM1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	360 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1. Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2 By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium By similarity.
Enzyme regulation	Activated by phosphorylation on tyrosine and threonine in response to insulin and NGF. Both phosphorylations are required for activity By similarity.
Subunit structure	Interacts with ARHGEF2, NISCH, MORG1 and HSF4 By similarity. Interacts (phosphorylated form) with CAV2 ('Tyr-19'-phosphorylated form); the interaction, promoted by insulin, leads to nuclear location and MAPK1 activation By similarity.
Domain	The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Post-translational modification	Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Cell cycle
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 360	359	Mitogen-activated protein kinase 1		PRO_0000186246
Regions
	Domain	25 – 313	289	Protein kinase
	Nucleotide binding	31 – 39	9	ATP By similarity
	Motif	185 – 187	3	TXY
	Compositional bias	2 – 9	8	Poly-Ala
Sites
	Active site	149	1	Proton acceptor By similarity
	Binding site	54	1	ATP By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	185	1	Phosphothreonine By similarity
	Modified residue	187	1	Phosphotyrosine By similarity
	Modified residue	190	1	Phosphothreonine By similarity
	Modified residue	202	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P46196-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: E85D0B2A4E9549DE	FASTA	360	41,376
	10 20 30 40 50 60 MAAAAAAGAG PEMVRGQVFD VGPRYTNLSY IGEGAYGMVC SAYDNVNKVR VAIKKISPFE 70 80 90 100 110 120 HQTYCQRTLR EIKILLRFRH ENIIGINDII RAPTIEQMKD VYIVQDLMET DLYKLLKTQH 130 140 150 160 170 180 LSNDHICYFL YQILRGLKYI HSANVLHRDL KPSNLLLNTT CDLKICDFGL ARVADPDHDH 190 200 210 220 230 240 TGFLTEYVAT RWYRAPEIML NSKGYTKSID IWSVGCILAE MLSNRPIFPG KHYLDQLNHI 250 260 270 280 290 300 LGILGSPSQE DLNCIINLKA RNYLLSLPHK NKVPWNRLFP NADSKALDLL DKMLTFNPHK 310 320 330 340 350 360 RIEVEQALAH PYLEQYYDPS DEPVAEAPFK FDMELDDLPK EKLKELIFEE TARFQPGYRS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of ERK2 from a bovine adrenal medulla cDNA library." Ely C.M., Cox M.E., Her J., Parsons S.J. Submitted (JUL-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Adrenal medulla.
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Fetal pons.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z14089 mRNA. Translation: CAA78467.1. BC133588 mRNA. Translation: AAI33589.2.
IPI	IPI00713672.
PIR	S25011.
RefSeq	NP_786987.1.
UniGene	Bt.167
3D structure databases
HSSP	HSSP built from PDB template 1PME based on UniProtKB P28482.
SMR	P46196. Positions 11-355, 12-356.
ModBase	Search...
Protein-protein interaction databases
STRING	P46196.
Genome annotation databases
GeneID	327672.
KEGG	bta:327672.
Organism-specific databases
CTD	327672.
Phylogenomic databases
HOVERGEN	P46196.
Enzyme and pathway databases
BRENDA	2.7.11.24. 251.
Family and domain databases
InterPro	IPR008349. Erk_1_2_MAPK. IPR003527. MAP_kinase_CS. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PRINTS	PR01770. ERK1ERK2MAPK.
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS01351. MAPK. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MK01_BOVIN

Accession

Primary (citable) accession number: P46196
Secondary accession number(s): A2VE60

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	January 23, 2007
Last modified:	September 22, 2009
This is version 86 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)