P46195 (KGUA_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Guanylate kinase
EC=2.7.4.8

Alternative name(s):
GMP kinase

Gene names

Name:	GUK1
Synonyms:	GUK

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	198 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential for recycling GMP and indirectly, cGMP.
Catalytic activity	ATP + GMP = ADP + GDP.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the guanylate kinase family. Contains 1 guanylate kinase-like domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	purine nucleotide metabolic process Inferred from mutant phenotype Ref.1. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW guanylate kinase activity Inferred from mutant phenotype Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 198	197	Guanylate kinase		PRO_0000170650
Regions
	Domain	4 – 186	183	Guanylate kinase-like
	Nucleotide binding	11 – 18	8	ATP By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylserine By similarity
	Modified residue	53	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P46195 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 9A153A0924C1F3AB	FASTA	198	21,910
	10 20 30 40 50 60 MSGPRPVVLS GPSGAGKSTL LKKLLQEHGS IFGFSVSHTT RDPRPGEENG KDYYFVTREV 70 80 90 100 110 120 MQRDIAAGDF IEHAEFSGNL YGTSKAAVRA VQAMNRICVL DVDLQGVRNI KKTDLRPIYI 130 140 150 160 170 180 FVQPPSLDVL EQRLRQRNTE TEESLAKRLA AARADMESSK EPGLFDLIIV NDSLDKAYWA 190 LKEALSEEIK KAQGTGQS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Guanylate kinase from bovine retina: isolation, primary structure, and expression in E. coli." Gaidarov I.O., Suslov O.N., Ovchinnikova T.V., Abdulaev N.G. Bioorg. Khim. 20:367-381(1994) [PubMed: 7911663] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Retina.
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Ileum.
[3]	"Enzymes of the cyclic GMP metabolism in bovine retina. I. Cloning and expression of the gene for guanylate kinase." Gaidarov I.O., Suslov O.N., Abdulaev N.G. FEBS Lett. 335:81-84(1993) [PubMed: 8243671] [Abstract] Cited for: PROTEIN SEQUENCE OF 10-17; 24-32; 35-82; 90-97; 121-126; 139-146; 149-154 AND 178-182.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X67029 mRNA. Translation: CAA47423.1. BC102478 mRNA. Translation: AAI02479.1.
IPI	IPI00716399.
PIR	S39447.
RefSeq	NP_001152884.1. NM_001159412.1. NP_001152885.1. NM_001159413.1. NP_776503.1. NM_174078.3.
UniGene	Bt.4287.
3D structure databases
ProteinModelPortal	P46195.
SMR	P46195. Positions 5-194.
ModBase	Search...
Protein-protein interaction databases
STRING	P46195.
Proteomic databases
PRIDE	P46195.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000036545; ENSBTAP00000036402; ENSBTAG00000014775. ENSBTAT00000066343; ENSBTAP00000054864; ENSBTAG00000014775.
GeneID	281217.
KEGG	bta:281217.
Organism-specific databases
CTD	2987.
Phylogenomic databases
eggNOG	maNOG18989.
GeneTree	ENSGT00560000076879.
HOVERGEN	HBG003344.
InParanoid	P46195.
OrthoDB	EOG4NS3CS.
PhylomeDB	P46195.
Family and domain databases
InterPro	IPR008144. Guanylate_kin. IPR008145. Guanylate_kin/L-typ_Ca_channel. IPR020590. Guanylate_kinase_CS. IPR017665. Guanylate_kinase_sub. [Graphical view]
KO	K00942.
Pfam	PF00625. Guanylate_kin. 1 hit. [Graphical view]
SMART	SM00072. GuKc. 1 hit. [Graphical view]
TIGRFAMs	TIGR03263. Guanyl_kin. 1 hit.
PROSITE	PS00856. GUANYLATE_KINASE_1. 1 hit. PS50052. GUANYLATE_KINASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KGUA_BOVIN

Accession

Primary (citable) accession number: P46195
Secondary accession number(s): Q3ZCE9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	January 23, 2007
Last modified:	November 16, 2011
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program