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Protein

Guanylate kinase

Gene

GUK1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Essential for recycling GMP and indirectly, cGMP.

Catalytic activityi

ATP + GMP = ADP + GDP.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei44 – 441By similarity
Active sitei137 – 1371By similarity
Active sitei148 – 1481By similarity
Binding sitei171 – 1711ATPBy similarity
Binding sitei172 – 1721ATP; via carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 188ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. guanylate kinase activity Source: UniProtKB

GO - Biological processi

  1. nucleotide phosphorylation Source: GOC
  2. purine nucleotide metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_274361. Abacavir metabolism.
REACT_305524. Synthesis and interconversion of nucleotide di- and triphosphates.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanylate kinase (EC:2.7.4.8)
Alternative name(s):
GMP kinase
Gene namesi
Name:GUK1
Synonyms:GUK
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Chromosome 7

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 198197Guanylate kinasePRO_0000170650Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiP46195.

Expressioni

Gene expression databases

ExpressionAtlasiP46195. baseline.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000019656.

Structurei

3D structure databases

ProteinModelPortaliP46195.
SMRiP46195. Positions 5-194.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 186183Guanylate kinase-likePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the guanylate kinase family.Curated
Contains 1 guanylate kinase-like domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0194.
GeneTreeiENSGT00730000110700.
HOGENOMiHOG000037640.
HOVERGENiHBG003344.
InParanoidiP46195.
KOiK00942.
OrthoDBiEOG7J1814.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR008145. GK/Ca_channel_bsu.
IPR008144. Guanylate_kin-like.
IPR017665. Guanylate_kinase.
IPR020590. Guanylate_kinase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00625. Guanylate_kin. 1 hit.
[Graphical view]
SMARTiSM00072. GuKc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR03263. guanyl_kin. 1 hit.
PROSITEiPS00856. GUANYLATE_KINASE_1. 1 hit.
PS50052. GUANYLATE_KINASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P46195-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGPRPVVLS GPSGAGKSTL LKKLLQEHGS IFGFSVSHTT RDPRPGEENG
60 70 80 90 100
KDYYFVTREV MQRDIAAGDF IEHAEFSGNL YGTSKAAVRA VQAMNRICVL
110 120 130 140 150
DVDLQGVRNI KKTDLRPIYI FVQPPSLDVL EQRLRQRNTE TEESLAKRLA
160 170 180 190
AARADMESSK EPGLFDLIIV NDSLDKAYWA LKEALSEEIK KAQGTGQS
Length:198
Mass (Da):21,910
Last modified:January 23, 2007 - v2
Checksum:i9A153A0924C1F3AB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67029 mRNA. Translation: CAA47423.1.
BC102478 mRNA. Translation: AAI02479.1.
PIRiS39447.
RefSeqiNP_001152884.1. NM_001159412.1.
NP_001152885.1. NM_001159413.1.
NP_776503.1. NM_174078.3.
XP_005208455.1. XM_005208398.2.
UniGeneiBt.4287.

Genome annotation databases

EnsembliENSBTAT00000036545; ENSBTAP00000036402; ENSBTAG00000014775.
ENSBTAT00000066343; ENSBTAP00000054864; ENSBTAG00000014775.
GeneIDi281217.
KEGGibta:281217.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67029 mRNA. Translation: CAA47423.1.
BC102478 mRNA. Translation: AAI02479.1.
PIRiS39447.
RefSeqiNP_001152884.1. NM_001159412.1.
NP_001152885.1. NM_001159413.1.
NP_776503.1. NM_174078.3.
XP_005208455.1. XM_005208398.2.
UniGeneiBt.4287.

3D structure databases

ProteinModelPortaliP46195.
SMRiP46195. Positions 5-194.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000019656.

Proteomic databases

PRIDEiP46195.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000036545; ENSBTAP00000036402; ENSBTAG00000014775.
ENSBTAT00000066343; ENSBTAP00000054864; ENSBTAG00000014775.
GeneIDi281217.
KEGGibta:281217.

Organism-specific databases

CTDi2987.

Phylogenomic databases

eggNOGiCOG0194.
GeneTreeiENSGT00730000110700.
HOGENOMiHOG000037640.
HOVERGENiHBG003344.
InParanoidiP46195.
KOiK00942.
OrthoDBiEOG7J1814.

Enzyme and pathway databases

ReactomeiREACT_274361. Abacavir metabolism.
REACT_305524. Synthesis and interconversion of nucleotide di- and triphosphates.

Miscellaneous databases

NextBioi20805269.

Gene expression databases

ExpressionAtlasiP46195. baseline.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR008145. GK/Ca_channel_bsu.
IPR008144. Guanylate_kin-like.
IPR017665. Guanylate_kinase.
IPR020590. Guanylate_kinase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00625. Guanylate_kin. 1 hit.
[Graphical view]
SMARTiSM00072. GuKc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR03263. guanyl_kin. 1 hit.
PROSITEiPS00856. GUANYLATE_KINASE_1. 1 hit.
PS50052. GUANYLATE_KINASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Guanylate kinase from bovine retina: isolation, primary structure, and expression in E. coli."
    Gaidarov I.O., Suslov O.N., Ovchinnikova T.V., Abdulaev N.G.
    Bioorg. Khim. 20:367-381(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Retina.
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Ileum.
  3. "Enzymes of the cyclic GMP metabolism in bovine retina. I. Cloning and expression of the gene for guanylate kinase."
    Gaidarov I.O., Suslov O.N., Abdulaev N.G.
    FEBS Lett. 335:81-84(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 10-17; 24-32; 35-82; 90-97; 121-126; 139-146; 149-154 AND 178-182.

Entry informationi

Entry nameiKGUA_BOVIN
AccessioniPrimary (citable) accession number: P46195
Secondary accession number(s): Q3ZCE9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: April 1, 2015
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.