Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P46195

- KGUA_BOVIN

UniProt

P46195 - KGUA_BOVIN

Protein

Guanylate kinase

Gene

GUK1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Essential for recycling GMP and indirectly, cGMP.

    Catalytic activityi

    ATP + GMP = ADP + GDP.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei44 – 441By similarity
    Active sitei137 – 1371By similarity
    Active sitei148 – 1481By similarity
    Binding sitei171 – 1711ATPBy similarity
    Binding sitei172 – 1721ATP; via carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi11 – 188ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. guanylate kinase activity Source: UniProtKB

    GO - Biological processi

    1. nucleotide phosphorylation Source: GOC
    2. purine nucleotide metabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_222543. Abacavir metabolism.
    REACT_223832. Synthesis and interconversion of nucleotide di- and triphosphates.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Guanylate kinase (EC:2.7.4.8)
    Alternative name(s):
    GMP kinase
    Gene namesi
    Name:GUK1
    Synonyms:GUK
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 7

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 198197Guanylate kinasePRO_0000170650Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PRIDEiP46195.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000019656.

    Structurei

    3D structure databases

    ProteinModelPortaliP46195.
    SMRiP46195. Positions 5-194.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 186183Guanylate kinase-likePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the guanylate kinase family.Curated
    Contains 1 guanylate kinase-like domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0194.
    GeneTreeiENSGT00730000110700.
    HOGENOMiHOG000037640.
    HOVERGENiHBG003344.
    InParanoidiP46195.
    KOiK00942.
    OrthoDBiEOG7J1814.

    Family and domain databases

    Gene3Di3.40.50.300. 3 hits.
    InterProiIPR008145. GK/Ca_channel_bsu.
    IPR008144. Guanylate_kin-like.
    IPR017665. Guanylate_kinase.
    IPR020590. Guanylate_kinase_CS.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00625. Guanylate_kin. 1 hit.
    [Graphical view]
    SMARTiSM00072. GuKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR03263. guanyl_kin. 1 hit.
    PROSITEiPS00856. GUANYLATE_KINASE_1. 1 hit.
    PS50052. GUANYLATE_KINASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P46195-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSGPRPVVLS GPSGAGKSTL LKKLLQEHGS IFGFSVSHTT RDPRPGEENG    50
    KDYYFVTREV MQRDIAAGDF IEHAEFSGNL YGTSKAAVRA VQAMNRICVL 100
    DVDLQGVRNI KKTDLRPIYI FVQPPSLDVL EQRLRQRNTE TEESLAKRLA 150
    AARADMESSK EPGLFDLIIV NDSLDKAYWA LKEALSEEIK KAQGTGQS 198
    Length:198
    Mass (Da):21,910
    Last modified:January 23, 2007 - v2
    Checksum:i9A153A0924C1F3AB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X67029 mRNA. Translation: CAA47423.1.
    BC102478 mRNA. Translation: AAI02479.1.
    PIRiS39447.
    RefSeqiNP_001152884.1. NM_001159412.1.
    NP_001152885.1. NM_001159413.1.
    NP_776503.1. NM_174078.3.
    XP_005208455.1. XM_005208398.1.
    UniGeneiBt.4287.

    Genome annotation databases

    EnsembliENSBTAT00000036545; ENSBTAP00000036402; ENSBTAG00000014775.
    ENSBTAT00000066343; ENSBTAP00000054864; ENSBTAG00000014775.
    GeneIDi281217.
    KEGGibta:281217.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X67029 mRNA. Translation: CAA47423.1 .
    BC102478 mRNA. Translation: AAI02479.1 .
    PIRi S39447.
    RefSeqi NP_001152884.1. NM_001159412.1.
    NP_001152885.1. NM_001159413.1.
    NP_776503.1. NM_174078.3.
    XP_005208455.1. XM_005208398.1.
    UniGenei Bt.4287.

    3D structure databases

    ProteinModelPortali P46195.
    SMRi P46195. Positions 5-194.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000019656.

    Proteomic databases

    PRIDEi P46195.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000036545 ; ENSBTAP00000036402 ; ENSBTAG00000014775 .
    ENSBTAT00000066343 ; ENSBTAP00000054864 ; ENSBTAG00000014775 .
    GeneIDi 281217.
    KEGGi bta:281217.

    Organism-specific databases

    CTDi 2987.

    Phylogenomic databases

    eggNOGi COG0194.
    GeneTreei ENSGT00730000110700.
    HOGENOMi HOG000037640.
    HOVERGENi HBG003344.
    InParanoidi P46195.
    KOi K00942.
    OrthoDBi EOG7J1814.

    Enzyme and pathway databases

    Reactomei REACT_222543. Abacavir metabolism.
    REACT_223832. Synthesis and interconversion of nucleotide di- and triphosphates.

    Miscellaneous databases

    NextBioi 20805269.

    Family and domain databases

    Gene3Di 3.40.50.300. 3 hits.
    InterProi IPR008145. GK/Ca_channel_bsu.
    IPR008144. Guanylate_kin-like.
    IPR017665. Guanylate_kinase.
    IPR020590. Guanylate_kinase_CS.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00625. Guanylate_kin. 1 hit.
    [Graphical view ]
    SMARTi SM00072. GuKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR03263. guanyl_kin. 1 hit.
    PROSITEi PS00856. GUANYLATE_KINASE_1. 1 hit.
    PS50052. GUANYLATE_KINASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Guanylate kinase from bovine retina: isolation, primary structure, and expression in E. coli."
      Gaidarov I.O., Suslov O.N., Ovchinnikova T.V., Abdulaev N.G.
      Bioorg. Khim. 20:367-381(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Retina.
    2. NIH - Mammalian Gene Collection (MGC) project
      Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Crossbred X Angus.
      Tissue: Ileum.
    3. "Enzymes of the cyclic GMP metabolism in bovine retina. I. Cloning and expression of the gene for guanylate kinase."
      Gaidarov I.O., Suslov O.N., Abdulaev N.G.
      FEBS Lett. 335:81-84(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 10-17; 24-32; 35-82; 90-97; 121-126; 139-146; 149-154 AND 178-182.

    Entry informationi

    Entry nameiKGUA_BOVIN
    AccessioniPrimary (citable) accession number: P46195
    Secondary accession number(s): Q3ZCE9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 94 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3