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P46194 (CP19A_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 22, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Aromatase

EC=1.14.14.14
Alternative name(s):
CYPXIX
Cytochrome P-450AROM
Cytochrome P450 19A1
Estrogen synthase
Gene names
Name:CYP19A1
Synonyms:CYP19
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length503 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the formation of aromatic C18 estrogens from C19 androgens.

Catalytic activity

Testosterone + 3 O2 + 3 reduced flavoproteins = 17-beta-estradiol + formate + 4 H2O + 3 oxidized flavoproteins.

Androst-4-ene-3,17-dione + 3 O2 + 3 reduced flavoproteins = estrone + formate + 4 H2O + 3 oxidized flavoproteins.

Cofactor

Heme group By similarity.

Subcellular location

Membrane; Peripheral membrane protein.

Sequence similarities

Belongs to the cytochrome P450 family.

Ontologies

Keywords
   Cellular componentMembrane
   LigandHeme
Iron
Metal-binding
   Molecular functionMonooxygenase
Oxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentmembrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionaromatase activity

Inferred from sequence or structural similarity. Source: UniProtKB

heme binding

Inferred from sequence or structural similarity. Source: UniProtKB

iron ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 503503Aromatase
PRO_0000051950

Sites

Metal binding4371Iron (heme axial ligand) By similarity
Binding site3091Substrate By similarity
Binding site3741Substrate; via amide nitrogen By similarity

Experimental info

Sequence conflict2161K → N in AAA62244. Ref.1
Sequence conflict2601E → A in AAA62244. Ref.1
Sequence conflict4361A → G in AAA62244. Ref.1
Sequence conflict4431T → A in AAA62244. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P46194 [UniParc].

Last modified August 29, 2001. Version 3.
Checksum: 47C70292D21AFF97

FASTA50358,090
        10         20         30         40         50         60 
MLLEVLNPRH YNVTSMVSEV VPIASIAILL LTGFLLLVWN YEDTSSIPGP SYFLGIGPLI 

        70         80         90        100        110        120 
SHCRFLWMGI GSACNYYNKM YGEFMRVWVC GEETLIISKS SSMFHVMKHS HYISRFGSKL 

       130        140        150        160        170        180 
GLQFIGMHEK GIIFNNNPAL WKAVRPFFTK ALSGPGLVRM VTICADSITK HLDRLEEVCN 

       190        200        210        220        230        240 
DLGYVDVLTL MRRIMLDTSN MLFLGIPLDE SAIVVKIQGY FDAWQALLLK PDIFFKISWL 

       250        260        270        280        290        300 
CRKYEKSVKD LKDAMEILIE EKRHRISTAE KLEDSIDFAT ELIFAEKRGE LTRENVNQCI 

       310        320        330        340        350        360 
LEMLIAAPDT MSVSVFFMLF LIAKHPQVEE AIIREIQTVV GERDIRIDDM QKLKVVENFI 

       370        380        390        400        410        420 
NESMRYQPVV DLVMRKALED DVIDGYPVKK GTNIILNLGR MHRLEFFPKP NEFTLENFAK 

       430        440        450        460        470        480 
NVPYRYFQPF GFGPRACAGK YITMVMMKVV LVTLLRRFHV QTLQGRCVEK MQKKNDLSLH 

       490        500 
PDETRDRLEM IFTPRNSDKC LER 

« Hide

References

[1]"Isolation and characterization of a complementary deoxyribonucleic acid insert encoding bovine aromatase cytochrome P450."
Hinshelwood M.M., Corbin C.J., Tsang P.C., Simpson E.R.
Endocrinology 133:1971-1977(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Ovary.
[2]"Novel aromatase transcripts from bovine placenta contain repeated sequence motifs."
Vanselow J., Furbass R.
Gene 154:281-286(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Placenta.
[3]"Tissue-specific expression of the bovine aromatase-encoding gene uses multiple transcriptional start sites and alternative first exons."
Furbass R., Kalbe C., Vanselow J.
Endocrinology 138:2813-2819(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]Furbass R.
Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO 443.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U18447 mRNA. Translation: AAA62244.1.
Z32741 mRNA. Translation: CAA83651.1.
Z69242 expand/collapse EMBL AC list , Z69243, Z69244, Z69245, Z69246, Z69247, Z69248, Z69249, Z69250 Genomic DNA. Translation: CAC12940.1.
PIRI46015.
RefSeqNP_776730.1. NM_174305.1.
UniGeneBt.4447.

3D structure databases

ProteinModelPortalP46194.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP46194.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID281740.
KEGGbta:281740.

Organism-specific databases

CTD1588.

Phylogenomic databases

eggNOGCOG2124.
HOGENOMHOG000111912.
HOVERGENHBG050750.
InParanoidP46194.
KOK07434.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20805660.

Entry information

Entry nameCP19A_BOVIN
AccessionPrimary (citable) accession number: P46194
Secondary accession number(s): Q29445, Q29453
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: August 29, 2001
Last modified: January 22, 2014
This is version 99 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families