P46194 (CP19A_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 19A1
EC=1.14.14.1

Alternative name(s):
Aromatase
CYPXIX
Cytochrome P-450AROM
Estrogen synthase

Gene names

Name:	CYP19A1
Synonyms:	CYP19

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	503 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the formation of aromatic C18 estrogens from C19 androgens.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Membrane; Peripheral membrane protein.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Membrane
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aromatase activity Inferred from sequence or structural similarity. Source: UniProtKB electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 503	503	Cytochrome P450 19A1		PRO_0000051950
Sites
	Metal binding	437	1	Iron (heme axial ligand) By similarity
	Binding site	309	1	Substrate By similarity
	Binding site	374	1	Substrate; via amide nitrogen By similarity
Amino acid modifications
	Modified residue	162	1	Phosphothreonine By similarity
Experimental info
	Sequence conflict	216	1	K → N in AAA62244. Ref.1
	Sequence conflict	260	1	E → A in AAA62244. Ref.1
	Sequence conflict	436	1	A → G in AAA62244. Ref.1
	Sequence conflict	443	1	T → A in AAA62244. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P46194 [UniParc]. Last modified August 29, 2001. Version 3. Checksum: 47C70292D21AFF97	FASTA	503	58,090
	10 20 30 40 50 60 MLLEVLNPRH YNVTSMVSEV VPIASIAILL LTGFLLLVWN YEDTSSIPGP SYFLGIGPLI 70 80 90 100 110 120 SHCRFLWMGI GSACNYYNKM YGEFMRVWVC GEETLIISKS SSMFHVMKHS HYISRFGSKL 130 140 150 160 170 180 GLQFIGMHEK GIIFNNNPAL WKAVRPFFTK ALSGPGLVRM VTICADSITK HLDRLEEVCN 190 200 210 220 230 240 DLGYVDVLTL MRRIMLDTSN MLFLGIPLDE SAIVVKIQGY FDAWQALLLK PDIFFKISWL 250 260 270 280 290 300 CRKYEKSVKD LKDAMEILIE EKRHRISTAE KLEDSIDFAT ELIFAEKRGE LTRENVNQCI 310 320 330 340 350 360 LEMLIAAPDT MSVSVFFMLF LIAKHPQVEE AIIREIQTVV GERDIRIDDM QKLKVVENFI 370 380 390 400 410 420 NESMRYQPVV DLVMRKALED DVIDGYPVKK GTNIILNLGR MHRLEFFPKP NEFTLENFAK 430 440 450 460 470 480 NVPYRYFQPF GFGPRACAGK YITMVMMKVV LVTLLRRFHV QTLQGRCVEK MQKKNDLSLH 490 500 PDETRDRLEM IFTPRNSDKC LER

References

[1]	"Isolation and characterization of a complementary deoxyribonucleic acid insert encoding bovine aromatase cytochrome P450." Hinshelwood M.M., Corbin C.J., Tsang P.C., Simpson E.R. Endocrinology 133:1971-1977(1993) [PubMed: 8404644] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Ovary.
[2]	"Novel aromatase transcripts from bovine placenta contain repeated sequence motifs." Vanselow J., Furbass R. Gene 154:281-286(1995) [PubMed: 7890178] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Placenta.
[3]	"Tissue-specific expression of the bovine aromatase-encoding gene uses multiple transcriptional start sites and alternative first exons." Furbass R., Kalbe C., Vanselow J. Endocrinology 138:2813-2819(1997) [PubMed: 9202222] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	Furbass R. Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 443.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18447 mRNA. Translation: AAA62244.1. Z32741 mRNA. Translation: CAA83651.1. Z69242 , Z69243, Z69244, Z69245, Z69246, Z69247, Z69248, Z69249, Z69250 Genomic DNA. Translation: CAC12940.1.
IPI	IPI00693174.
PIR	I46015.
RefSeq	NP_776730.1. NM_174305.1.
UniGene	Bt.4447.
3D structure databases
ProteinModelPortal	P46194.
ModBase	Search...
Protein-protein interaction databases
STRING	P46194.
Proteomic databases
PRIDE	P46194.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	281740.
KEGG	bta:281740.
Organism-specific databases
CTD	1588.
Phylogenomic databases
eggNOG	maNOG09449.
HOVERGEN	HBG050750.
InParanoid	P46194.
OrthoDB	EOG4ZCT4K.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
KO	K07434.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP19A_BOVIN

Accession

Primary (citable) accession number: P46194
Secondary accession number(s): Q29445, Q29453

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	August 29, 2001
Last modified:	November 16, 2011
This is version 86 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program