Reviewed, UniProtKB/Swiss-Prot P46170 (DFB12_BOVIN)

Names and origin

Protein names

Recommended name:
    Beta-defensin 12
Alternative name(s):
    BNDB-12
    BNBD-12

Gene names

Name:

DEFB12

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	38 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Has bactericidal activity. Active against E.coli ML35 and S.aureus 502A.
Subcellular location	Secreted.
Tissue specificity	Neutrophilic granules.
Sequence similarities	Belongs to the beta-defensin family.

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Antibiotic Antimicrobial Defensin
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Peptide

1 – 38

38

Beta-defensin 12

PRO_0000044727

Amino acid modifications

Disulfide bond

5 ↔ 34

Ref.2

Disulfide bond

12 ↔ 27

Ref.2

Disulfide bond

17 ↔ 35

Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

38

Helix Strand Turn

Details...

Turn

6 – 9

4

Beta strand

10 – 16

7

Beta strand

21 – 27

7

Turn

28 – 31

4

Beta strand

32 – 36

5

Sequences

	Sequence		Length	Mass (Da)
	P46170-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: FD0710704504E3E7	FASTA	38	4,106
	10 20 30 GPLSCGRNGG VCIPIRCPVP MRQIGTCFGR PVKCCRSW

References

[1]	"Purification, primary structures, and antibacterial activities of beta-defensins, a new family of antimicrobial peptides from bovine neutrophils." Selsted M.E., Tang Y.-Q., Morris W.L., McGuire P.A., Novotny M.J., Smith W., Henschen A.H., Cullor J.S. J. Biol. Chem. 268:6641-6648(1993) [PubMed: 8454635] [Abstract] Cited for: PROTEIN SEQUENCE. Strain: Hereford. Tissue: Neutrophil.
[2]	"Characterization of the disulfide motif in BNBD-12, an antimicrobial beta-defensin peptide from bovine neutrophils." Tang Y.-Q., Selsted M.E. J. Biol. Chem. 268:6649-6653(1993) [PubMed: 8454636] [Abstract] Cited for: DISULFIDE BONDS.
[3]	"Solution structure of bovine neutrophil beta-defensin-12: the peptide fold of the beta-defensins is identical to that of the classical defensins." Zimmermann G.R., Legault P., Selsted M.E., Pardi A. Biochemistry 34:13663-13671(1995) [PubMed: 7577957] [Abstract] Cited for: STRUCTURE BY NMR.

Cross-references

Sequence databases

IPI

IPI00692831.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BNB	NMR	-	A	2-38	[»]

DisProt

DP00209.

ModBase

Search...

Phylogenomic databases

HOVERGEN

P46170.

Family and domain databases

InterPro

IPR001855. Defensin_beta.
IPR006080. Defensin_mammal.
[Graphical view]

Pfam

PF00711. Defensin_beta. 1 hit.
[Graphical view]

SMART

SM00048. DEFSN. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

DFB12_BOVIN

Accession

Primary (citable) accession number: P46170

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)