P46157 (GLL1A_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Gallinacin-1 alpha Short name=Gal-1 alpha Alternative name(s): Antimicrobial peptide CHP2 Chicken heterophil peptide 2
Organism	Gallus gallus (Chicken) [Reference proteome]
Taxonomic identifier	9031 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Sauropsida › Sauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	65 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has bactericidal activity. Potent activity against E.coli ML-35, L.monocytogenes EGD and C.albicans.
Subcellular location	Secreted. Cytoplasmic granule.
Sequence similarities	Belongs to the beta-defensin family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Antibiotic Antimicrobial Defensin Fungicide
PTM	Disulfide bond
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW defense response to fungus Inferred from electronic annotation. Source: UniProtKB-KW killing of cells of other organism Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Propeptide	20 – 25	6			PRO_0000007010
	Peptide	26 – 64	39	Gallinacin-1 alpha Ref.3		PRO_0000007011
Amino acid modifications
	Disulfide bond	31 ↔ 59		By similarity
	Disulfide bond	38 ↔ 53		By similarity
	Disulfide bond	43 ↔ 60		By similarity
Experimental info
	Sequence conflict	52	1	K → L AA sequence Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P46157 [UniParc]. Last modified August 30, 2005. Version 2. Checksum: BC835B79574F2ECE	FASTA	65	7,286
	10 20 30 40 50 60 MRIVYLLLPF ILLLAQGAAG SSQALGRKSD CFRKNGFCAF LKCPYLTLIS GKCSRFHLCC KRIWG

References

[1]	"Gallinacin-3, an inducible epithelial beta-defensin in the chicken." Zhao C., Nguyen T., Liu L., Sacco R.E., Brogden K.A., Lehrer R.I. Infect. Immun. 69:2684-2691(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Trachea.
[2]	"Chicken beta-defensin in China chicken breeds." Cao Y., Chen Y., Bi Y., Xie Q., Chen J. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Guangxi Huang and Qingyuan Ma.
[3]	"Gallinacins: cysteine-rich antimicrobial peptides of chicken leukocytes." Harwig S.S.L., Swiderek K.M., Kokryakov V.N., Tan L., Lee T.D., Panyutich E.A., Aleshina G.M., Shamova O.V., Lehrer R.I. FEBS Lett. 342:281-285(1994) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 26-64. Strain: Cross Broiler-6. Tissue: Leukocyte.
[4]	"Isolation of antimicrobial peptides from avian heterophils." Evans E.W., Beach G.G., Wunderlich J., Harmon B.G. J. Leukoc. Biol. 56:661-665(1994) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 26-59.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF181951 mRNA. Translation: AAG09211.1. DQ858337 mRNA. Translation: ABI48253.1. DQ858297 mRNA. Translation: ABI48213.1.
IPI	IPI00593185.
PIR	S43282.
UniGene	Gga.176.
3D structure databases
ProteinModelPortal	P46157.
ModBase	Search...
Protein family/group databases
TCDB	1.C.85.3.1. pore-forming beta,-defensin (beta,-Defensin) family.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG004222.
Family and domain databases
InterPro	IPR001855. Defensin_beta-typ. [Graphical view]
Pfam	PF00711. Defensin_beta. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLL1A_CHICK

Accession

Primary (citable) accession number: P46157
Secondary accession number(s): P80390, Q09MR8, Q9DG59

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	August 30, 2005
Last modified:	April 3, 2013
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program