Reviewed, UniProtKB/Swiss-Prot P46155 (TOP1_KLEAE)

Names and origin

Protein names

Recommended name:
    DNA topoisomerase 1
    EC=5.99.1.2
Alternative name(s):
    DNA topoisomerase I
    Omega-protein
    Relaxing enzyme
    Untwisting enzyme
    Swivelase

Gene names

Name:

topA

Organism

Klebsiella aerogenes

Taxonomic identifier

28451 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	18 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
Subunit structure	Monomer By similarity.
Miscellaneous	When a topoisomerase transiently breaks a DNA backbone bond, it simultaneously forms a protein-DNA link, in which a tyrosyl oxygen in the enzyme is joined to a DNA phosphorus at one end of the enzyme-severed DNA strand.
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family.

Ontologies

Keywords
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 18	›18	DNA topoisomerase 1		PRO_0000145152
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P46155-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 8C1C81238FF0EFA4	FASTA	18	2,043
	10 ATGWSAFFID GKWTEAKK

References

[1]

"Characterization of the CysB protein of Klebsiella aerogenes: direct evidence that N-acetylserine rather than O-acetylserine serves as the inducer of the cysteine regulon." Lynch A.S., Tyrrell R., Smerdon S.J., Briggs G.S., Wilkinson A.J. Biochem. J. 299:129-136(1994) [PubMed: 8166630] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NCTC 418 / ATCC 15380.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X78729 Genomic DNA. No translation available.
3D structure databases
HSSP	HSSP built from PDB template 1YUA based on UniProtKB P06612.
ModBase	Search...
Enzyme and pathway databases
BRENDA	5.99.1.2. 366.
Family and domain databases
InterPro	IPR000380. Topo_IA_core. IPR013263. TopoI_Znr_bac. [Graphical view]
Pfam	PF08272. Topo_Zn_Ribbon. 1 hit. [Graphical view]
PROSITE	PS00396. TOPOISOMERASE_I_PROK. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TOP1_KLEAE

Accession

Primary (citable) accession number: P46155

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)