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P46155 (TOP1_ENTAE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA topoisomerase 1

EC=5.99.1.2
Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene names
Name:topA
OrganismEnterobacter aerogenes (Aerobacter aerogenes)
Taxonomic identifier548 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacter

Protein attributes

Sequence length18 AA.
Sequence statusFragment.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.

Subunit structure

Monomer By similarity.

Sequence similarities

Belongs to the type IA topoisomerase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – 18›18DNA topoisomerase 1
PRO_0000145152

Experimental info

Non-terminal residue11

Sequences

Sequence LengthMass (Da)Tools
P46155 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 8C1C81238FF0EFA4

FASTA182,043
        10 
ATGWSAFFID GKWTEAKK 

« Hide

References

[1]"Characterization of the CysB protein of Klebsiella aerogenes: direct evidence that N-acetylserine rather than O-acetylserine serves as the inducer of the cysteine regulon."
Lynch A.S., Tyrrell R., Smerdon S.J., Briggs G.S., Wilkinson A.J.
Biochem. J. 299:129-136(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: NCTC 418 / ATCC 15380.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X78729 Genomic DNA. No translation available.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR013263. TopoI_Znr_bac.
[Graphical view]
PfamPF08272. Topo_Zn_Ribbon. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTOP1_ENTAE
AccessionPrimary (citable) accession number: P46155
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 9, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families