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Protein

Transcription factor GATA-4

Gene

Gata4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development (By similarity). In cooperation with TBX5, it binds to cardiac super-enhancers and promotes cardiomyocyte gene expression, while it downregulates endocardial and endothelial gene expression (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement (By similarity). Required during testicular development (By similarity). Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression (PubMed:15329343). Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (PubMed:15329343). May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, spingosine-1-phosphate lyase (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri216 – 240GATA-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri270 – 294GATA-type 2PROSITE-ProRule annotationAdd BLAST25

GO - Molecular functioni

  • activating transcription factor binding Source: BHF-UCL
  • chromatin binding Source: GO_Central
  • co-SMAD binding Source: Ensembl
  • DNA binding Source: BHF-UCL
  • enhancer sequence-specific DNA binding Source: UniProtKB
  • NFAT protein binding Source: RGD
  • protein kinase binding Source: RGD
  • RNA polymerase II regulatory region sequence-specific DNA binding Source: GO_Central
  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: RGD
  • RNA polymerase II transcription factor binding Source: GO_Central
  • sequence-specific DNA binding Source: RGD
  • transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding Source: GO_Central
  • transcription coactivator activity Source: Ensembl
  • transcription factor activity, RNA polymerase II transcription factor binding Source: Ensembl
  • transcription factor activity, sequence-specific DNA binding Source: BHF-UCL
  • transcription factor binding Source: RGD
  • transcription regulatory region DNA binding Source: BHF-UCL
  • zinc ion binding Source: RGD

GO - Biological processi

  • atrial septum secundum morphogenesis Source: Ensembl
  • cardiac muscle cell differentiation Source: RGD
  • cell growth involved in cardiac muscle cell development Source: RGD
  • cellular response to glucose stimulus Source: RGD
  • endocardial cushion development Source: Ensembl
  • heart development Source: RGD
  • intestinal epithelial cell differentiation Source: Ensembl
  • male gonad development Source: Ensembl
  • negative regulation of autophagy Source: RGD
  • positive regulation of BMP signaling pathway Source: BHF-UCL
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: RGD
  • regulation of cardiac muscle cell contraction Source: RGD
  • regulation of transcription, DNA-templated Source: RGD
  • response to drug Source: Ensembl
  • response to mechanical stimulus Source: RGD
  • response to vitamin A Source: RGD
  • transdifferentiation Source: RGD

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-RNO-983231. Factors involved in megakaryocyte development and platelet production.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor GATA-4
Alternative name(s):
DNA-binding protein GATA-GT2
GATA-binding factor 4
Gene namesi
Name:Gata4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 15

Organism-specific databases

RGDi2665. Gata4.

Subcellular locationi

  • Nucleus By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000834151 – 440Transcription factor GATA-4Add BLAST440

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei299N6-methyllysine; by EZH2By similarity1

Post-translational modificationi

Methylation at Lys-299 attenuates transcriptional activity.By similarity

Keywords - PTMi

Methylation

Proteomic databases

PaxDbiP46152.

PTM databases

iPTMnetiP46152.
PhosphoSitePlusiP46152.

Expressioni

Tissue specificityi

Expressed predominantly in gastric mucosa and at much lower levels in the intestine. Also expressed in testis.

Gene expression databases

BgeeiENSRNOG00000010708.
GenevisibleiP46152. RN.

Interactioni

Subunit structurei

Interacts with the homeobox domain of NKX2-5 through its C-terminal zinc finger. Also interacts with JARID2 which represses its ability to activate transcription of ANF. Interacts with NFATC4 and LMCD1. Forms a complex made of CDK9, CCNT1/cyclin-T1, EP300 and GATA4 that stimulates hypertrophy in cardiomyocytes. Interacts with NR5A1, ZFPM2 and TBX5. Interacts with ZNF260. Interacts with TBX18.By similarity1 Publication

GO - Molecular functioni

  • activating transcription factor binding Source: BHF-UCL
  • co-SMAD binding Source: Ensembl
  • NFAT protein binding Source: RGD
  • protein kinase binding Source: RGD
  • RNA polymerase II transcription factor binding Source: GO_Central
  • transcription factor binding Source: RGD

Protein-protein interaction databases

BioGridi248477. 4 interactors.
DIPiDIP-60791N.
IntActiP46152. 1 interactor.
STRINGi10116.ENSRNOP00000014320.

Structurei

3D structure databases

ProteinModelPortaliP46152.
SMRiP46152.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi118 – 126Poly-Ala9
Compositional biasi174 – 181Poly-Ala8
Compositional biasi275 – 279Poly-Thr5
Compositional biasi353 – 358Poly-Ser6

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri216 – 240GATA-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri270 – 294GATA-type 2PROSITE-ProRule annotationAdd BLAST25

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1601. Eukaryota.
COG5641. LUCA.
GeneTreeiENSGT00760000119221.
HOGENOMiHOG000047700.
HOVERGENiHBG051703.
InParanoidiP46152.
KOiK09183.
OMAiSPGWSQA.
OrthoDBiEOG091G0AUR.
PhylomeDBiP46152.

Family and domain databases

Gene3Di3.30.50.10. 1 hit.
InterProiView protein in InterPro
IPR008013. GATA_N.
IPR016375. TF_GATA_4/5/6.
IPR000679. Znf_GATA.
IPR013088. Znf_NHR/GATA.
PfamiView protein in Pfam
PF00320. GATA. 2 hits.
PF05349. GATA-N. 1 hit.
PIRSFiPIRSF003028. TF_GATA_4/5/6. 1 hit.
PRINTSiPR00619. GATAZNFINGER.
SMARTiView protein in SMART
SM00401. ZnF_GATA. 2 hits.
PROSITEiView protein in PROSITE
PS00344. GATA_ZN_FINGER_1. 2 hits.
PS50114. GATA_ZN_FINGER_2. 2 hits.

Sequencei

Sequence statusi: Complete.

P46152-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYQSLAMAAN HGPPPGAYEA GGPGAFMHSA GAASSPVYVP TPRVPSSVLG
60 70 80 90 100
LSYLQGGGSG AASGATSGGS SGAGPSGAGP GTQQGSPGWS QAGAEGAAYT
110 120 130 140 150
PPPVSPRFSF PGTTGSLAAA AAAAAAREAA AYSSSGGAAG AGLAGREQYG
160 170 180 190 200
RPGFAGSYSS PYPAYMADVG ASWAAAAAAS AGPFDSPVLH SLPGRANPAR
210 220 230 240 250
HPNLDMFDDF SEGRECVNCG AMSTPLWRRD GTGHYLCNAC GLYHKMNGIN
260 270 280 290 300
RPLIKPQRRL SASRRVGLSC ANCQTTTTTL WRRNAEGEPV CNACGLYMKL
310 320 330 340 350
HGVPRPLAMR KEGIQTRKRK PKNLNKSKTP AGPPGESLPP SSGASSNSSN
360 370 380 390 400
ATSSSSSSEE MRPIKTEPGL SSHYGHSSSM SQTFSTVSGH GSSIHPVLSA
410 420 430 440
LKLSPQGYPS PVTQTSQASS KQDSWNSLVL ADSHGDIITA
Length:440
Mass (Da):44,597
Last modified:November 1, 1995 - v1
Checksum:i7C594FA95080AD43
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22761 mRNA. Translation: AAA16159.1.
PIRiI61183.
RefSeqiNP_653331.1. NM_144730.1.
UniGeneiRn.26251.

Genome annotation databases

EnsembliENSRNOT00000014320; ENSRNOP00000014320; ENSRNOG00000010708.
GeneIDi54254.
KEGGirno:54254.
UCSCiRGD:2665. rat.

Similar proteinsi

Entry informationi

Entry nameiGATA4_RAT
AccessioniPrimary (citable) accession number: P46152
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: August 30, 2017
This is version 148 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome