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Protein

Transcription factor GATA-4

Gene

Gata4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement (By similarity). Required during testicular development (By similarity). Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression (PubMed:15329343). Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (PubMed:15329343). May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, spingosine-1-phosphate lyase (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri216 – 24025GATA-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri270 – 29425GATA-type 2PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • activating transcription factor binding Source: BHF-UCL
  • DNA binding Source: BHF-UCL
  • enhancer sequence-specific DNA binding Source: UniProtKB
  • NFAT protein binding Source: RGD
  • protein kinase binding Source: RGD
  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: RGD
  • sequence-specific DNA binding Source: RGD
  • transcription coactivator activity Source: Ensembl
  • transcription factor activity, RNA polymerase II transcription factor binding Source: Ensembl
  • transcription factor activity, sequence-specific DNA binding Source: BHF-UCL
  • transcription factor binding Source: UniProtKB
  • transcription regulatory region DNA binding Source: BHF-UCL
  • zinc ion binding Source: RGD

GO - Biological processi

  • atrial septum secundum morphogenesis Source: Ensembl
  • cardiac muscle cell differentiation Source: RGD
  • cell growth involved in cardiac muscle cell development Source: RGD
  • cellular response to glucose stimulus Source: RGD
  • endocardial cushion development Source: Ensembl
  • heart development Source: RGD
  • intestinal epithelial cell differentiation Source: Ensembl
  • male gonad development Source: Ensembl
  • negative regulation of autophagy Source: RGD
  • positive regulation of BMP signaling pathway Source: BHF-UCL
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: RGD
  • regulation of cardiac muscle cell contraction Source: RGD
  • regulation of transcription, DNA-templated Source: RGD
  • response to drug Source: Ensembl
  • response to mechanical stimulus Source: RGD
  • response to vitamin A Source: RGD
  • transcription, DNA-templated Source: UniProtKB-KW
  • transdifferentiation Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-RNO-983231. Factors involved in megakaryocyte development and platelet production.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor GATA-4
Alternative name(s):
DNA-binding protein GATA-GT2
GATA-binding factor 4
Gene namesi
Name:Gata4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 15

Organism-specific databases

RGDi2665. Gata4.

Subcellular locationi

  • Nucleus By similarity

GO - Cellular componenti

  • nucleus Source: RGD
  • RNA polymerase II transcription factor complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 440440Transcription factor GATA-4PRO_0000083415Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei299 – 2991N6-methyllysine; by EZH2By similarity

Post-translational modificationi

Methylation at Lys-299 attenuates transcriptional activity.By similarity

Keywords - PTMi

Methylation

Proteomic databases

PaxDbiP46152.

PTM databases

iPTMnetiP46152.
PhosphoSiteiP46152.

Expressioni

Tissue specificityi

Expressed predominantly in gastric mucosa and at much lower levels in the intestine. Also expressed in testis.

Gene expression databases

GenevisibleiP46152. RN.

Interactioni

Subunit structurei

Interacts with the homeobox domain of NKX2-5 through its C-terminal zinc finger. Also interacts with JARID2 which represses its ability to activate transcription of ANF. Interacts with NFATC4 and LMCD1. Forms a complex made of CDK9, CCNT1/cyclin-T1, EP300 and GATA4 that stimulates hypertrophy in cardiomyocytes. Interacts with NR5A1, ZFPM2 and TBX5. Interacts with ZNF260. Interacts with TBX18.By similarity1 Publication

GO - Molecular functioni

  • activating transcription factor binding Source: BHF-UCL
  • NFAT protein binding Source: RGD
  • protein kinase binding Source: RGD
  • transcription factor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi248477. 4 interactions.
DIPiDIP-60791N.
IntActiP46152. 1 interaction.
STRINGi10116.ENSRNOP00000014320.

Structurei

3D structure databases

ProteinModelPortaliP46152.
SMRiP46152. Positions 211-255, 264-319.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi118 – 1269Poly-Ala
Compositional biasi174 – 1818Poly-Ala
Compositional biasi275 – 2795Poly-Thr
Compositional biasi353 – 3586Poly-Ser

Sequence similaritiesi

Contains 2 GATA-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri216 – 24025GATA-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri270 – 29425GATA-type 2PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1601. Eukaryota.
COG5641. LUCA.
GeneTreeiENSGT00760000119221.
HOGENOMiHOG000047700.
HOVERGENiHBG051703.
InParanoidiP46152.
KOiK09183.
OMAiSPGWSQA.
OrthoDBiEOG7CCBRF.
PhylomeDBiP46152.

Family and domain databases

Gene3Di3.30.50.10. 2 hits.
InterProiIPR028436. GATA_4/pnr.
IPR008013. GATA_N.
IPR016375. TF_GATA_4/5/6.
IPR000679. Znf_GATA.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PANTHERiPTHR10071:SF154. PTHR10071:SF154. 1 hit.
PfamiPF00320. GATA. 2 hits.
PF05349. GATA-N. 1 hit.
[Graphical view]
PIRSFiPIRSF003028. TF_GATA_4/5/6. 1 hit.
PRINTSiPR00619. GATAZNFINGER.
SMARTiSM00401. ZnF_GATA. 2 hits.
[Graphical view]
PROSITEiPS00344. GATA_ZN_FINGER_1. 2 hits.
PS50114. GATA_ZN_FINGER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P46152-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYQSLAMAAN HGPPPGAYEA GGPGAFMHSA GAASSPVYVP TPRVPSSVLG
60 70 80 90 100
LSYLQGGGSG AASGATSGGS SGAGPSGAGP GTQQGSPGWS QAGAEGAAYT
110 120 130 140 150
PPPVSPRFSF PGTTGSLAAA AAAAAAREAA AYSSSGGAAG AGLAGREQYG
160 170 180 190 200
RPGFAGSYSS PYPAYMADVG ASWAAAAAAS AGPFDSPVLH SLPGRANPAR
210 220 230 240 250
HPNLDMFDDF SEGRECVNCG AMSTPLWRRD GTGHYLCNAC GLYHKMNGIN
260 270 280 290 300
RPLIKPQRRL SASRRVGLSC ANCQTTTTTL WRRNAEGEPV CNACGLYMKL
310 320 330 340 350
HGVPRPLAMR KEGIQTRKRK PKNLNKSKTP AGPPGESLPP SSGASSNSSN
360 370 380 390 400
ATSSSSSSEE MRPIKTEPGL SSHYGHSSSM SQTFSTVSGH GSSIHPVLSA
410 420 430 440
LKLSPQGYPS PVTQTSQASS KQDSWNSLVL ADSHGDIITA
Length:440
Mass (Da):44,597
Last modified:November 1, 1995 - v1
Checksum:i7C594FA95080AD43
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22761 mRNA. Translation: AAA16159.1.
PIRiI61183.
RefSeqiNP_653331.1. NM_144730.1.
UniGeneiRn.26251.

Genome annotation databases

EnsembliENSRNOT00000014320; ENSRNOP00000014320; ENSRNOG00000010708.
GeneIDi54254.
KEGGirno:54254.
UCSCiRGD:2665. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22761 mRNA. Translation: AAA16159.1.
PIRiI61183.
RefSeqiNP_653331.1. NM_144730.1.
UniGeneiRn.26251.

3D structure databases

ProteinModelPortaliP46152.
SMRiP46152. Positions 211-255, 264-319.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248477. 4 interactions.
DIPiDIP-60791N.
IntActiP46152. 1 interaction.
STRINGi10116.ENSRNOP00000014320.

PTM databases

iPTMnetiP46152.
PhosphoSiteiP46152.

Proteomic databases

PaxDbiP46152.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000014320; ENSRNOP00000014320; ENSRNOG00000010708.
GeneIDi54254.
KEGGirno:54254.
UCSCiRGD:2665. rat.

Organism-specific databases

CTDi2626.
RGDi2665. Gata4.

Phylogenomic databases

eggNOGiKOG1601. Eukaryota.
COG5641. LUCA.
GeneTreeiENSGT00760000119221.
HOGENOMiHOG000047700.
HOVERGENiHBG051703.
InParanoidiP46152.
KOiK09183.
OMAiSPGWSQA.
OrthoDBiEOG7CCBRF.
PhylomeDBiP46152.

Enzyme and pathway databases

ReactomeiR-RNO-983231. Factors involved in megakaryocyte development and platelet production.

Miscellaneous databases

NextBioi610762.
PROiP46152.

Gene expression databases

GenevisibleiP46152. RN.

Family and domain databases

Gene3Di3.30.50.10. 2 hits.
InterProiIPR028436. GATA_4/pnr.
IPR008013. GATA_N.
IPR016375. TF_GATA_4/5/6.
IPR000679. Znf_GATA.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PANTHERiPTHR10071:SF154. PTHR10071:SF154. 1 hit.
PfamiPF00320. GATA. 2 hits.
PF05349. GATA-N. 1 hit.
[Graphical view]
PIRSFiPIRSF003028. TF_GATA_4/5/6. 1 hit.
PRINTSiPR00619. GATAZNFINGER.
SMARTiSM00401. ZnF_GATA. 2 hits.
[Graphical view]
PROSITEiPS00344. GATA_ZN_FINGER_1. 2 hits.
PS50114. GATA_ZN_FINGER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Gastric DNA-binding proteins recognize upstream sequence motifs of parietal cell-specific genes."
    Tamura S., Wang X.H., Maeda M., Futai M.
    Proc. Natl. Acad. Sci. U.S.A. 90:10876-10880(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Stomach.
  2. "SMAD-mediated modulation of YY1 activity regulates the BMP response and cardiac-specific expression of a GATA4/5/6-dependent chick Nkx2.5 enhancer."
    Lee K.H., Evans S., Ruan T.Y., Lassar A.B.
    Development 131:4709-4723(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DNA-BINDING.
  3. "The zinc finger-only protein Zfp260 is a novel cardiac regulator and a nuclear effector of alpha1-adrenergic signaling."
    Debrus S., Rahbani L., Marttila M., Delorme B., Paradis P., Nemer M.
    Mol. Cell. Biol. 25:8669-8682(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ZNF260.

Entry informationi

Entry nameiGATA4_RAT
AccessioniPrimary (citable) accession number: P46152
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 11, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.