P46151 (MTHR1_YEAST) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 29, 2013.
Version 113.
History...
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Methylenetetrahydrofolate reductase 1 EC=1.5.1.20 | ||||||
| Gene names |
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| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome] | ||||||
| Taxonomic identifier | 559292 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › ![]() |
Protein attributes
| Sequence length | 657 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Catalytic activity | 5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H. |
| Cofactor | FAD By similarity. |
| Pathway | |
| Sequence similarities | Belongs to the methylenetetrahydrofolate reductase family. |
Ontologies
| Keywords | |
|---|---|
| Ligand | FAD Flavoprotein NADP |
| Molecular function | Oxidoreductase |
| PTM | Phosphoprotein |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | methionine metabolic process Inferred from electronic annotation. Source: InterPro tetrahydrofolate interconversionInferred from electronic annotation. Source: UniProtKB-UniPathway |
| Molecular_function | methylenetetrahydrofolate reductase (NADPH) activity Inferred from direct assay PubMed 10600168. Source: SGD |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| MET13 | P53128 | 2 | EBI-11567,EBI-11572 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 657 | 657 | Methylenetetrahydrofolate reductase 1 | PRO_0000190255 | |||||
Amino acid modifications | |||||||||
| Modified residue | 120 | 1 | Phosphoserine Ref.7 | ||||||
| Modified residue | 301 | 1 | Phosphoserine Ref.7 | ||||||
| Modified residue | 358 | 1 | Phosphoserine Ref.6 Ref.7 | ||||||
| Modified residue | 359 | 1 | Phosphoserine Ref.6 Ref.7 | ||||||
| Modified residue | 407 | 1 | Phosphothreonine Ref.7 | ||||||
Experimental info | |||||||||
| Sequence conflict | 110 – 111 | 2 | DP → NL in K02070. Ref.3 | ||||||
| Sequence conflict | 115 – 116 | 2 | ED → VV in K02070. Ref.3 | ||||||
| Sequence conflict | 119 | 1 | D → V in K02070. Ref.3 | ||||||
| Sequence conflict | 124 – 131 | 8 | ESPFKYAV → RLLNMRLF in K02070. Ref.3 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. Hani J.Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972. |
| [2] | Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c. |
| [3] | "Molecular cloning and nucleotide sequence analysis of the Saccharomyces cerevisiae RAD1 gene." Yang E., Friedberg E.C. Mol. Cell. Biol. 4:2161-2169(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-131. |
| [4] | "Human methylenetetrahydrofolate reductase: isolation of cDNA, mapping and mutation identification." Goyette P., Sumner J.S., Milos R., Duncan A.M.V., Rosenblatt D.S., Matthews R.G., Rozen R. Nat. Genet. 7:195-200(1994) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION. |
| [5] | Erratum Goyette P., Sumner J.S., Milos R., Duncan A.M.V., Rosenblatt D.S., Matthews R.G., Rozen R. Nat. Genet. 7:551-551(1994) [PubMed] [Europe PMC] [Abstract] |
| [6] | "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-358 AND SER-359, MASS SPECTROMETRY. |
| [7] | "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-120; SER-301; SER-358; SER-359 AND THR-407, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | U36624 Genomic DNA. Translation: AAB68164.1. K02070 Genomic DNA. No translation available. BK006949 Genomic DNA. Translation: DAA11405.1. |
| PIR | S63459. |
| RefSeq | NP_015302.1. NM_001183837.1. |
3D structure databases | |
| ProteinModelPortal | P46151. |
| SMR | P46151. Positions 1-300. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-2825N. |
| IntAct | P46151. 5 interactions. |
| MINT | MINT-1699985. |
| STRING | 4932.YPL023C. |
Proteomic databases | |
| PaxDb | P46151. |
| PeptideAtlas | P46151. |
| PRIDE | P46151. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblFungi | YPL023C; YPL023C; YPL023C. |
| GeneID | 856084. |
| KEGG | sce:YPL023C. |
Organism-specific databases | |
| CYGD | YPL023c. |
| SGD | S000005944. MET12. |
Phylogenomic databases | |
| eggNOG | COG0685. |
| GeneTree | ENSGT00650000094052. |
| HOGENOM | HOG000246234. |
| KO | K00297. |
| OMA | YGEIDGY. |
| OrthoDB | EOG444PTG. |
Enzyme and pathway databases | |
| BioCyc | YEAST:YPL023C-MONOMER. |
| UniPathway | UPA00193. |
Gene expression databases | |
| Genevestigator | P46151. |
| GermOnline | YPL023C. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR004621. Fadh2_euk. IPR003171. Mehydrof_redctse. [Graphical view] |
| Pfam | PF02219. MTHFR. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR00677. fadh2_euk. 1 hit. |
| ProtoNet | Search... |
Other | |
| NextBio | 981098. |
Entry information
| Entry name | MTHR1_YEAST | ||||||||
| Accession | Primary (citable) accession number: P46151 Secondary accession number(s): D6W3Y9 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Fungal Protein Annotation Program | ||||||||
Relevant documents
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome XVI Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with
