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Protein

Methylenetetrahydrofolate reductase 1

Gene

MET12

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactori

FADBy similarity

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei18Proton donor/acceptorBy similarity1
Binding sitei78FADBy similarity1
Binding sitei110SubstrateBy similarity1
Binding sitei152FADBy similarity1
Binding sitei171FADBy similarity1
Binding sitei178FADBy similarity1
Binding sitei189SubstrateBy similarity1
Binding sitei286SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi18 – 23NADBy similarity6
Nucleotide bindingi49 – 50NAD and FADBy similarity2
Nucleotide bindingi108 – 110FADBy similarity3
Nucleotide bindingi129 – 130FADBy similarity2

GO - Molecular functioni

  • methylenetetrahydrofolate reductase (NAD(P)H) activity Source: SGD

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandFAD, Flavoprotein, NADP

Enzyme and pathway databases

BioCyciYEAST:YPL023C-MONOMER
UniPathwayiUPA00193

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate reductase 1 (EC:1.5.1.20)
Gene namesi
Name:MET12
Ordered Locus Names:YPL023C
ORF Names:LPB8C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL023C
SGDiS000005944 MET12

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001902551 – 657Methylenetetrahydrofolate reductase 1Add BLAST657

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei120PhosphoserineCombined sources1
Modified residuei301PhosphoserineCombined sources1
Modified residuei358PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP46151
PaxDbiP46151
PRIDEiP46151

PTM databases

iPTMnetiP46151

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
MET13P531282EBI-11567,EBI-11572

Protein-protein interaction databases

BioGridi36154, 134 interactors
DIPiDIP-2825N
IntActiP46151, 5 interactors
MINTiP46151
STRINGi4932.YPL023C

Structurei

Secondary structure

1657
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 9Combined sources7
Beta strandi14 – 19Combined sources6
Helixi25 – 38Combined sources14
Helixi39 – 41Combined sources3
Beta strandi46 – 48Combined sources3
Helixi52 – 54Combined sources3
Helixi57 – 71Combined sources15
Beta strandi75 – 80Combined sources6
Helixi86 – 98Combined sources13
Beta strandi103 – 107Combined sources5
Helixi116 – 118Combined sources3
Helixi130 – 141Combined sources12
Helixi142 – 144Combined sources3
Beta strandi145 – 151Combined sources7
Helixi168 – 180Combined sources13
Beta strandi185 – 190Combined sources6
Helixi194 – 207Combined sources14
Beta strandi210 – 212Combined sources3
Beta strandi214 – 218Combined sources5
Helixi224 – 234Combined sources11
Helixi240 – 243Combined sources4
Helixi248 – 251Combined sources4
Helixi254 – 275Combined sources22
Turni276 – 278Combined sources3
Beta strandi282 – 286Combined sources5
Helixi292 – 300Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
6FNUX-ray1.56A1-302[»]
ProteinModelPortaliP46151
SMRiP46151
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000246234
InParanoidiP46151
KOiK00297
OMAiNVTWGAG
OrthoDBiEOG092C12V2

Family and domain databases

CDDicd00537 MTHFR, 1 hit
InterProiView protein in InterPro
IPR029041 FAD-linked_oxidoreductase-like
IPR004621 Fadh2_euk
IPR003171 Mehydrof_redctse
PfamiView protein in Pfam
PF02219 MTHFR, 1 hit
SUPFAMiSSF51730 SSF51730, 1 hit
TIGRFAMsiTIGR00677 fadh2_euk, 1 hit

Sequencei

Sequence statusi: Complete.

P46151-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIRDLYHAR ASPFISLEFF PPKTELGTRN LMERMHRMTA LDPLFITVTW
60 70 80 90 100
GAGGTTAEKT LTLASLAQQT LNIPVCMHLT CTNTEKAIID DALDRCYNAG
110 120 130 140 150
IRNILALRGD PPIGEDWLDS QSNESPFKYA VDLVRYIKQS YGDKFCVGVA
160 170 180 190 200
AYPEGHCEGE AEGHEQDPLK DLVYLKEKVE AGADFVITQL FYDVEKFLTF
210 220 230 240 250
EMLFRERISQ DLPLFPGLMP INSYLLFHRA AKLSHASIPP AILSRFPPEI
260 270 280 290 300
QSDDNAVKSI GVDILIELIQ EIYQRTSGRI KGFHFYTLNL EKAIAQIVSQ
310 320 330 340 350
SPVLSHIVNE SSEEEGEDET SGEIGSIENV PIEDADGDIV LDDSNEETVA
360 370 380 390 400
NRKRRRHSSL DSAKLIFNRA IVTEKGLRYN NENGSMPSKK ALISISKGHG
410 420 430 440 450
TLGRDATWDE FPNGRFGDSR SPAYGEIDGY GPSIKVSKSK ALELWGIPKT
460 470 480 490 500
IGDLKDIFIK YLEGSTDAIP WSDLGLSAET ALIQEELIQL NYRGYLTLAS
510 520 530 540 550
QPATNATLSS DKIFGWGPAK GRLYQKAFVE MFIHRQQWET TLKPKLDHYG
560 570 580 590 600
RRKFSYYAGD SSGSFETNLD PHSSSVVTWG VFPNSPVKQT TIIEEESFKA
610 620 630 640 650
WRDEAFSIWS EWAKLFPRNT PANILLRLVH KDYCLVSIVH HDFKETDELW

EMLLDQA
Length:657
Mass (Da):73,942
Last modified:October 1, 1996 - v2
Checksum:iD75612EA92D67500
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti110 – 111DP → NL in K02070 (PubMed:6095044).Curated2
Sequence conflicti115 – 116ED → VV in K02070 (PubMed:6095044).Curated2
Sequence conflicti119D → V in K02070 (PubMed:6095044).Curated1
Sequence conflicti124 – 131ESPFKYAV → RLLNMRLF in K02070 (PubMed:6095044).Curated8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U36624 Genomic DNA Translation: AAB68164.1
K02070 Genomic DNA No translation available.
BK006949 Genomic DNA Translation: DAA11405.1
PIRiS63459
RefSeqiNP_015302.1, NM_001183837.1

Genome annotation databases

EnsemblFungiiYPL023C; YPL023C; YPL023C
GeneIDi856084
KEGGisce:YPL023C

Similar proteinsi

Entry informationi

Entry nameiMTHR1_YEAST
AccessioniPrimary (citable) accession number: P46151
Secondary accession number(s): D6W3Y9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 157 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

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