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P46151

- MTHR1_YEAST

UniProt

P46151 - MTHR1_YEAST

Protein

Methylenetetrahydrofolate reductase 1

Gene

MET12

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 2 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

    Cofactori

    FAD.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei18 – 181Proton donor/acceptorBy similarity
    Binding sitei78 – 781FADBy similarity
    Binding sitei110 – 1101SubstrateBy similarity
    Binding sitei152 – 1521FADBy similarity
    Binding sitei171 – 1711FADBy similarity
    Binding sitei178 – 1781FADBy similarity
    Binding sitei189 – 1891SubstrateBy similarity
    Binding sitei286 – 2861SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi18 – 236NADBy similarity
    Nucleotide bindingi49 – 502NAD and FADBy similarity
    Nucleotide bindingi108 – 1103FADBy similarity
    Nucleotide bindingi129 – 1302FADBy similarity

    GO - Molecular functioni

    1. methylenetetrahydrofolate reductase (NAD(P)H) activity Source: SGD
    2. protein binding Source: IntAct

    GO - Biological processi

    1. methionine metabolic process Source: InterPro
    2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    FAD, Flavoprotein, NADP

    Enzyme and pathway databases

    BioCyciYEAST:YPL023C-MONOMER.
    UniPathwayiUPA00193.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methylenetetrahydrofolate reductase 1 (EC:1.5.1.20)
    Gene namesi
    Name:MET12
    Ordered Locus Names:YPL023C
    ORF Names:LPB8C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPL023c.
    SGDiS000005944. MET12.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 657657Methylenetetrahydrofolate reductase 1PRO_0000190255Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei120 – 1201Phosphoserine1 Publication
    Modified residuei301 – 3011Phosphoserine1 Publication
    Modified residuei358 – 3581Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP46151.
    PaxDbiP46151.
    PeptideAtlasiP46151.
    PRIDEiP46151.

    Expressioni

    Gene expression databases

    GenevestigatoriP46151.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    MET13P531282EBI-11567,EBI-11572

    Protein-protein interaction databases

    BioGridi36154. 50 interactions.
    DIPiDIP-2825N.
    IntActiP46151. 5 interactions.
    MINTiMINT-1699985.
    STRINGi4932.YPL023C.

    Structurei

    3D structure databases

    ProteinModelPortaliP46151.
    SMRiP46151. Positions 1-300.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0685.
    HOGENOMiHOG000246234.
    KOiK00297.
    OMAiNVTWGAG.
    OrthoDBiEOG7M0P15.

    Family and domain databases

    Gene3Di3.20.20.220. 1 hit.
    InterProiIPR029041. FAD-linked_oxidoreductase-like.
    IPR004621. Fadh2_euk.
    IPR003171. Mehydrof_redctse.
    [Graphical view]
    PfamiPF02219. MTHFR. 1 hit.
    [Graphical view]
    SUPFAMiSSF51730. SSF51730. 1 hit.
    TIGRFAMsiTIGR00677. fadh2_euk. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P46151-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSIRDLYHAR ASPFISLEFF PPKTELGTRN LMERMHRMTA LDPLFITVTW    50
    GAGGTTAEKT LTLASLAQQT LNIPVCMHLT CTNTEKAIID DALDRCYNAG 100
    IRNILALRGD PPIGEDWLDS QSNESPFKYA VDLVRYIKQS YGDKFCVGVA 150
    AYPEGHCEGE AEGHEQDPLK DLVYLKEKVE AGADFVITQL FYDVEKFLTF 200
    EMLFRERISQ DLPLFPGLMP INSYLLFHRA AKLSHASIPP AILSRFPPEI 250
    QSDDNAVKSI GVDILIELIQ EIYQRTSGRI KGFHFYTLNL EKAIAQIVSQ 300
    SPVLSHIVNE SSEEEGEDET SGEIGSIENV PIEDADGDIV LDDSNEETVA 350
    NRKRRRHSSL DSAKLIFNRA IVTEKGLRYN NENGSMPSKK ALISISKGHG 400
    TLGRDATWDE FPNGRFGDSR SPAYGEIDGY GPSIKVSKSK ALELWGIPKT 450
    IGDLKDIFIK YLEGSTDAIP WSDLGLSAET ALIQEELIQL NYRGYLTLAS 500
    QPATNATLSS DKIFGWGPAK GRLYQKAFVE MFIHRQQWET TLKPKLDHYG 550
    RRKFSYYAGD SSGSFETNLD PHSSSVVTWG VFPNSPVKQT TIIEEESFKA 600
    WRDEAFSIWS EWAKLFPRNT PANILLRLVH KDYCLVSIVH HDFKETDELW 650
    EMLLDQA 657
    Length:657
    Mass (Da):73,942
    Last modified:October 1, 1996 - v2
    Checksum:iD75612EA92D67500
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti110 – 1112DP → NL in K02070. (PubMed:6095044)Curated
    Sequence conflicti115 – 1162ED → VV in K02070. (PubMed:6095044)Curated
    Sequence conflicti119 – 1191D → V in K02070. (PubMed:6095044)Curated
    Sequence conflicti124 – 1318ESPFKYAV → RLLNMRLF in K02070. (PubMed:6095044)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U36624 Genomic DNA. Translation: AAB68164.1.
    K02070 Genomic DNA. No translation available.
    BK006949 Genomic DNA. Translation: DAA11405.1.
    PIRiS63459.
    RefSeqiNP_015302.1. NM_001183837.1.

    Genome annotation databases

    EnsemblFungiiYPL023C; YPL023C; YPL023C.
    GeneIDi856084.
    KEGGisce:YPL023C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U36624 Genomic DNA. Translation: AAB68164.1 .
    K02070 Genomic DNA. No translation available.
    BK006949 Genomic DNA. Translation: DAA11405.1 .
    PIRi S63459.
    RefSeqi NP_015302.1. NM_001183837.1.

    3D structure databases

    ProteinModelPortali P46151.
    SMRi P46151. Positions 1-300.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36154. 50 interactions.
    DIPi DIP-2825N.
    IntActi P46151. 5 interactions.
    MINTi MINT-1699985.
    STRINGi 4932.YPL023C.

    Proteomic databases

    MaxQBi P46151.
    PaxDbi P46151.
    PeptideAtlasi P46151.
    PRIDEi P46151.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPL023C ; YPL023C ; YPL023C .
    GeneIDi 856084.
    KEGGi sce:YPL023C.

    Organism-specific databases

    CYGDi YPL023c.
    SGDi S000005944. MET12.

    Phylogenomic databases

    eggNOGi COG0685.
    HOGENOMi HOG000246234.
    KOi K00297.
    OMAi NVTWGAG.
    OrthoDBi EOG7M0P15.

    Enzyme and pathway databases

    UniPathwayi UPA00193 .
    BioCyci YEAST:YPL023C-MONOMER.

    Miscellaneous databases

    NextBioi 981098.

    Gene expression databases

    Genevestigatori P46151.

    Family and domain databases

    Gene3Di 3.20.20.220. 1 hit.
    InterProi IPR029041. FAD-linked_oxidoreductase-like.
    IPR004621. Fadh2_euk.
    IPR003171. Mehydrof_redctse.
    [Graphical view ]
    Pfami PF02219. MTHFR. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51730. SSF51730. 1 hit.
    TIGRFAMsi TIGR00677. fadh2_euk. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Molecular cloning and nucleotide sequence analysis of the Saccharomyces cerevisiae RAD1 gene."
      Yang E., Friedberg E.C.
      Mol. Cell. Biol. 4:2161-2169(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-131.
    4. "Human methylenetetrahydrofolate reductase: isolation of cDNA, mapping and mutation identification."
      Goyette P., Sumner J.S., Milos R., Duncan A.M.V., Rosenblatt D.S., Matthews R.G., Rozen R.
      Nat. Genet. 7:195-200(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION.
    5. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-120 AND SER-301, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    6. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-358, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiMTHR1_YEAST
    AccessioniPrimary (citable) accession number: P46151
    Secondary accession number(s): D6W3Y9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 124 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3