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P46151

- MTHR1_YEAST

UniProt

P46151 - MTHR1_YEAST

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Protein

Methylenetetrahydrofolate reductase 1

Gene

MET12

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactori

FADBy similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei18 – 181Proton donor/acceptorBy similarity
Binding sitei78 – 781FADBy similarity
Binding sitei110 – 1101SubstrateBy similarity
Binding sitei152 – 1521FADBy similarity
Binding sitei171 – 1711FADBy similarity
Binding sitei178 – 1781FADBy similarity
Binding sitei189 – 1891SubstrateBy similarity
Binding sitei286 – 2861SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi18 – 236NADBy similarity
Nucleotide bindingi49 – 502NAD and FADBy similarity
Nucleotide bindingi108 – 1103FADBy similarity
Nucleotide bindingi129 – 1302FADBy similarity

GO - Molecular functioni

  1. methylenetetrahydrofolate reductase (NAD(P)H) activity Source: SGD

GO - Biological processi

  1. methionine metabolic process Source: InterPro
  2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, NADP

Enzyme and pathway databases

BioCyciYEAST:YPL023C-MONOMER.
ReactomeiREACT_261783. Metabolism of folate and pterines.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate reductase 1 (EC:1.5.1.20)
Gene namesi
Name:MET12
Ordered Locus Names:YPL023C
ORF Names:LPB8C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XVI

Organism-specific databases

CYGDiYPL023c.
SGDiS000005944. MET12.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 657657Methylenetetrahydrofolate reductase 1PRO_0000190255Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei120 – 1201Phosphoserine1 Publication
Modified residuei301 – 3011Phosphoserine1 Publication
Modified residuei358 – 3581Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP46151.
PaxDbiP46151.
PeptideAtlasiP46151.
PRIDEiP46151.

Expressioni

Gene expression databases

GenevestigatoriP46151.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
MET13P531282EBI-11567,EBI-11572

Protein-protein interaction databases

BioGridi36154. 52 interactions.
DIPiDIP-2825N.
IntActiP46151. 5 interactions.
MINTiMINT-1699985.
STRINGi4932.YPL023C.

Structurei

3D structure databases

ProteinModelPortaliP46151.
SMRiP46151. Positions 1-300.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0685.
HOGENOMiHOG000246234.
InParanoidiP46151.
KOiK00297.
OMAiNVTWGAG.
OrthoDBiEOG7M0P15.

Family and domain databases

Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR004621. Fadh2_euk.
IPR003171. Mehydrof_redctse.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00677. fadh2_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

P46151-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSIRDLYHAR ASPFISLEFF PPKTELGTRN LMERMHRMTA LDPLFITVTW
60 70 80 90 100
GAGGTTAEKT LTLASLAQQT LNIPVCMHLT CTNTEKAIID DALDRCYNAG
110 120 130 140 150
IRNILALRGD PPIGEDWLDS QSNESPFKYA VDLVRYIKQS YGDKFCVGVA
160 170 180 190 200
AYPEGHCEGE AEGHEQDPLK DLVYLKEKVE AGADFVITQL FYDVEKFLTF
210 220 230 240 250
EMLFRERISQ DLPLFPGLMP INSYLLFHRA AKLSHASIPP AILSRFPPEI
260 270 280 290 300
QSDDNAVKSI GVDILIELIQ EIYQRTSGRI KGFHFYTLNL EKAIAQIVSQ
310 320 330 340 350
SPVLSHIVNE SSEEEGEDET SGEIGSIENV PIEDADGDIV LDDSNEETVA
360 370 380 390 400
NRKRRRHSSL DSAKLIFNRA IVTEKGLRYN NENGSMPSKK ALISISKGHG
410 420 430 440 450
TLGRDATWDE FPNGRFGDSR SPAYGEIDGY GPSIKVSKSK ALELWGIPKT
460 470 480 490 500
IGDLKDIFIK YLEGSTDAIP WSDLGLSAET ALIQEELIQL NYRGYLTLAS
510 520 530 540 550
QPATNATLSS DKIFGWGPAK GRLYQKAFVE MFIHRQQWET TLKPKLDHYG
560 570 580 590 600
RRKFSYYAGD SSGSFETNLD PHSSSVVTWG VFPNSPVKQT TIIEEESFKA
610 620 630 640 650
WRDEAFSIWS EWAKLFPRNT PANILLRLVH KDYCLVSIVH HDFKETDELW

EMLLDQA
Length:657
Mass (Da):73,942
Last modified:October 1, 1996 - v2
Checksum:iD75612EA92D67500
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti110 – 1112DP → NL in K02070. (PubMed:6095044)Curated
Sequence conflicti115 – 1162ED → VV in K02070. (PubMed:6095044)Curated
Sequence conflicti119 – 1191D → V in K02070. (PubMed:6095044)Curated
Sequence conflicti124 – 1318ESPFKYAV → RLLNMRLF in K02070. (PubMed:6095044)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U36624 Genomic DNA. Translation: AAB68164.1.
K02070 Genomic DNA. No translation available.
BK006949 Genomic DNA. Translation: DAA11405.1.
PIRiS63459.
RefSeqiNP_015302.1. NM_001183837.1.

Genome annotation databases

EnsemblFungiiYPL023C; YPL023C; YPL023C.
GeneIDi856084.
KEGGisce:YPL023C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U36624 Genomic DNA. Translation: AAB68164.1 .
K02070 Genomic DNA. No translation available.
BK006949 Genomic DNA. Translation: DAA11405.1 .
PIRi S63459.
RefSeqi NP_015302.1. NM_001183837.1.

3D structure databases

ProteinModelPortali P46151.
SMRi P46151. Positions 1-300.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36154. 52 interactions.
DIPi DIP-2825N.
IntActi P46151. 5 interactions.
MINTi MINT-1699985.
STRINGi 4932.YPL023C.

Proteomic databases

MaxQBi P46151.
PaxDbi P46151.
PeptideAtlasi P46151.
PRIDEi P46151.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YPL023C ; YPL023C ; YPL023C .
GeneIDi 856084.
KEGGi sce:YPL023C.

Organism-specific databases

CYGDi YPL023c.
SGDi S000005944. MET12.

Phylogenomic databases

eggNOGi COG0685.
HOGENOMi HOG000246234.
InParanoidi P46151.
KOi K00297.
OMAi NVTWGAG.
OrthoDBi EOG7M0P15.

Enzyme and pathway databases

UniPathwayi UPA00193 .
BioCyci YEAST:YPL023C-MONOMER.
Reactomei REACT_261783. Metabolism of folate and pterines.

Miscellaneous databases

NextBioi 981098.

Gene expression databases

Genevestigatori P46151.

Family and domain databases

Gene3Di 3.20.20.220. 1 hit.
InterProi IPR029041. FAD-linked_oxidoreductase-like.
IPR004621. Fadh2_euk.
IPR003171. Mehydrof_redctse.
[Graphical view ]
Pfami PF02219. MTHFR. 1 hit.
[Graphical view ]
SUPFAMi SSF51730. SSF51730. 1 hit.
TIGRFAMsi TIGR00677. fadh2_euk. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Molecular cloning and nucleotide sequence analysis of the Saccharomyces cerevisiae RAD1 gene."
    Yang E., Friedberg E.C.
    Mol. Cell. Biol. 4:2161-2169(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-131.
  4. "Human methylenetetrahydrofolate reductase: isolation of cDNA, mapping and mutation identification."
    Goyette P., Sumner J.S., Milos R., Duncan A.M.V., Rosenblatt D.S., Matthews R.G., Rozen R.
    Nat. Genet. 7:195-200(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  5. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-120 AND SER-301, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-358, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMTHR1_YEAST
AccessioniPrimary (citable) accession number: P46151
Secondary accession number(s): D6W3Y9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 1, 1996
Last modified: November 26, 2014
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3