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P46151

- MTHR1_YEAST

UniProt

P46151 - MTHR1_YEAST

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Protein
Methylenetetrahydrofolate reductase 1
Gene
MET12, YPL023C, LPB8C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactori

FAD By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei18 – 181Proton donor/acceptor By similarity
Binding sitei78 – 781FAD By similarity
Binding sitei110 – 1101Substrate By similarity
Binding sitei152 – 1521FAD By similarity
Binding sitei171 – 1711FAD By similarity
Binding sitei178 – 1781FAD By similarity
Binding sitei189 – 1891Substrate By similarity
Binding sitei286 – 2861Substrate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi18 – 236NAD By similarity
Nucleotide bindingi49 – 502NAD and FAD By similarity
Nucleotide bindingi108 – 1103FAD By similarity
Nucleotide bindingi129 – 1302FAD By similarity

GO - Molecular functioni

  1. methylenetetrahydrofolate reductase (NAD(P)H) activity Source: SGD
  2. protein binding Source: IntAct
Complete GO annotation...

GO - Biological processi

  1. methionine metabolic process Source: InterPro
  2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, NADP

Enzyme and pathway databases

BioCyciYEAST:YPL023C-MONOMER.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate reductase 1 (EC:1.5.1.20)
Gene namesi
Name:MET12
Ordered Locus Names:YPL023C
ORF Names:LPB8C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XVI

Organism-specific databases

CYGDiYPL023c.
SGDiS000005944. MET12.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 657657Methylenetetrahydrofolate reductase 1
PRO_0000190255Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei120 – 1201Phosphoserine1 Publication
Modified residuei301 – 3011Phosphoserine1 Publication
Modified residuei358 – 3581Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP46151.
PaxDbiP46151.
PeptideAtlasiP46151.
PRIDEiP46151.

Expressioni

Gene expression databases

GenevestigatoriP46151.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
MET13P531282EBI-11567,EBI-11572

Protein-protein interaction databases

BioGridi36154. 50 interactions.
DIPiDIP-2825N.
IntActiP46151. 5 interactions.
MINTiMINT-1699985.
STRINGi4932.YPL023C.

Structurei

3D structure databases

ProteinModelPortaliP46151.
SMRiP46151. Positions 1-300.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0685.
HOGENOMiHOG000246234.
KOiK00297.
OMAiNVTWGAG.
OrthoDBiEOG7M0P15.

Family and domain databases

Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR004621. Fadh2_euk.
IPR003171. Mehydrof_redctse.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00677. fadh2_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

P46151-1 [UniParc]FASTAAdd to Basket

« Hide

MSIRDLYHAR ASPFISLEFF PPKTELGTRN LMERMHRMTA LDPLFITVTW    50
GAGGTTAEKT LTLASLAQQT LNIPVCMHLT CTNTEKAIID DALDRCYNAG 100
IRNILALRGD PPIGEDWLDS QSNESPFKYA VDLVRYIKQS YGDKFCVGVA 150
AYPEGHCEGE AEGHEQDPLK DLVYLKEKVE AGADFVITQL FYDVEKFLTF 200
EMLFRERISQ DLPLFPGLMP INSYLLFHRA AKLSHASIPP AILSRFPPEI 250
QSDDNAVKSI GVDILIELIQ EIYQRTSGRI KGFHFYTLNL EKAIAQIVSQ 300
SPVLSHIVNE SSEEEGEDET SGEIGSIENV PIEDADGDIV LDDSNEETVA 350
NRKRRRHSSL DSAKLIFNRA IVTEKGLRYN NENGSMPSKK ALISISKGHG 400
TLGRDATWDE FPNGRFGDSR SPAYGEIDGY GPSIKVSKSK ALELWGIPKT 450
IGDLKDIFIK YLEGSTDAIP WSDLGLSAET ALIQEELIQL NYRGYLTLAS 500
QPATNATLSS DKIFGWGPAK GRLYQKAFVE MFIHRQQWET TLKPKLDHYG 550
RRKFSYYAGD SSGSFETNLD PHSSSVVTWG VFPNSPVKQT TIIEEESFKA 600
WRDEAFSIWS EWAKLFPRNT PANILLRLVH KDYCLVSIVH HDFKETDELW 650
EMLLDQA 657
Length:657
Mass (Da):73,942
Last modified:October 1, 1996 - v2
Checksum:iD75612EA92D67500
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti110 – 1112DP → NL in K02070. 1 Publication
Sequence conflicti115 – 1162ED → VV in K02070. 1 Publication
Sequence conflicti119 – 1191D → V in K02070. 1 Publication
Sequence conflicti124 – 1318ESPFKYAV → RLLNMRLF in K02070. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U36624 Genomic DNA. Translation: AAB68164.1.
K02070 Genomic DNA. No translation available.
BK006949 Genomic DNA. Translation: DAA11405.1.
PIRiS63459.
RefSeqiNP_015302.1. NM_001183837.1.

Genome annotation databases

EnsemblFungiiYPL023C; YPL023C; YPL023C.
GeneIDi856084.
KEGGisce:YPL023C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U36624 Genomic DNA. Translation: AAB68164.1 .
K02070 Genomic DNA. No translation available.
BK006949 Genomic DNA. Translation: DAA11405.1 .
PIRi S63459.
RefSeqi NP_015302.1. NM_001183837.1.

3D structure databases

ProteinModelPortali P46151.
SMRi P46151. Positions 1-300.
ModBasei Search...

Protein-protein interaction databases

BioGridi 36154. 50 interactions.
DIPi DIP-2825N.
IntActi P46151. 5 interactions.
MINTi MINT-1699985.
STRINGi 4932.YPL023C.

Proteomic databases

MaxQBi P46151.
PaxDbi P46151.
PeptideAtlasi P46151.
PRIDEi P46151.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YPL023C ; YPL023C ; YPL023C .
GeneIDi 856084.
KEGGi sce:YPL023C.

Organism-specific databases

CYGDi YPL023c.
SGDi S000005944. MET12.

Phylogenomic databases

eggNOGi COG0685.
HOGENOMi HOG000246234.
KOi K00297.
OMAi NVTWGAG.
OrthoDBi EOG7M0P15.

Enzyme and pathway databases

UniPathwayi UPA00193 .
BioCyci YEAST:YPL023C-MONOMER.

Miscellaneous databases

NextBioi 981098.

Gene expression databases

Genevestigatori P46151.

Family and domain databases

Gene3Di 3.20.20.220. 1 hit.
InterProi IPR029041. FAD-linked_oxidoreductase-like.
IPR004621. Fadh2_euk.
IPR003171. Mehydrof_redctse.
[Graphical view ]
Pfami PF02219. MTHFR. 1 hit.
[Graphical view ]
SUPFAMi SSF51730. SSF51730. 1 hit.
TIGRFAMsi TIGR00677. fadh2_euk. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Molecular cloning and nucleotide sequence analysis of the Saccharomyces cerevisiae RAD1 gene."
    Yang E., Friedberg E.C.
    Mol. Cell. Biol. 4:2161-2169(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-131.
  4. "Human methylenetetrahydrofolate reductase: isolation of cDNA, mapping and mutation identification."
    Goyette P., Sumner J.S., Milos R., Duncan A.M.V., Rosenblatt D.S., Matthews R.G., Rozen R.
    Nat. Genet. 7:195-200(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  5. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-120 AND SER-301, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-358, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMTHR1_YEAST
AccessioniPrimary (citable) accession number: P46151
Secondary accession number(s): D6W3Y9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3

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