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Protein

Methylenetetrahydrofolate reductase 1

Gene

MET12

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactori

FADBy similarity

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei18Proton donor/acceptorBy similarity1
Binding sitei78FADBy similarity1
Binding sitei110SubstrateBy similarity1
Binding sitei152FADBy similarity1
Binding sitei171FADBy similarity1
Binding sitei178FADBy similarity1
Binding sitei189SubstrateBy similarity1
Binding sitei286SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi18 – 23NADBy similarity6
Nucleotide bindingi49 – 50NAD and FADBy similarity2
Nucleotide bindingi108 – 110FADBy similarity3
Nucleotide bindingi129 – 130FADBy similarity2

GO - Molecular functioni

  • methylenetetrahydrofolate reductase (NAD(P)H) activity Source: SGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, NADP

Enzyme and pathway databases

BioCyciYEAST:YPL023C-MONOMER.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate reductase 1 (EC:1.5.1.20)
Gene namesi
Name:MET12
Ordered Locus Names:YPL023C
ORF Names:LPB8C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL023C.
SGDiS000005944. MET12.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001902551 – 657Methylenetetrahydrofolate reductase 1Add BLAST657

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei120PhosphoserineCombined sources1
Modified residuei301PhosphoserineCombined sources1
Modified residuei358PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP46151.
PRIDEiP46151.

PTM databases

iPTMnetiP46151.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
MET13P531282EBI-11567,EBI-11572

Protein-protein interaction databases

BioGridi36154. 51 interactors.
DIPiDIP-2825N.
IntActiP46151. 5 interactors.
MINTiMINT-1699985.

Structurei

3D structure databases

ProteinModelPortaliP46151.
SMRiP46151.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000246234.
InParanoidiP46151.
KOiK00297.
OMAiNVTWGAG.
OrthoDBiEOG092C12V2.

Family and domain databases

CDDicd00537. MTHFR. 1 hit.
Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR004621. Fadh2_euk.
IPR003171. Mehydrof_redctse.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00677. fadh2_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

P46151-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIRDLYHAR ASPFISLEFF PPKTELGTRN LMERMHRMTA LDPLFITVTW
60 70 80 90 100
GAGGTTAEKT LTLASLAQQT LNIPVCMHLT CTNTEKAIID DALDRCYNAG
110 120 130 140 150
IRNILALRGD PPIGEDWLDS QSNESPFKYA VDLVRYIKQS YGDKFCVGVA
160 170 180 190 200
AYPEGHCEGE AEGHEQDPLK DLVYLKEKVE AGADFVITQL FYDVEKFLTF
210 220 230 240 250
EMLFRERISQ DLPLFPGLMP INSYLLFHRA AKLSHASIPP AILSRFPPEI
260 270 280 290 300
QSDDNAVKSI GVDILIELIQ EIYQRTSGRI KGFHFYTLNL EKAIAQIVSQ
310 320 330 340 350
SPVLSHIVNE SSEEEGEDET SGEIGSIENV PIEDADGDIV LDDSNEETVA
360 370 380 390 400
NRKRRRHSSL DSAKLIFNRA IVTEKGLRYN NENGSMPSKK ALISISKGHG
410 420 430 440 450
TLGRDATWDE FPNGRFGDSR SPAYGEIDGY GPSIKVSKSK ALELWGIPKT
460 470 480 490 500
IGDLKDIFIK YLEGSTDAIP WSDLGLSAET ALIQEELIQL NYRGYLTLAS
510 520 530 540 550
QPATNATLSS DKIFGWGPAK GRLYQKAFVE MFIHRQQWET TLKPKLDHYG
560 570 580 590 600
RRKFSYYAGD SSGSFETNLD PHSSSVVTWG VFPNSPVKQT TIIEEESFKA
610 620 630 640 650
WRDEAFSIWS EWAKLFPRNT PANILLRLVH KDYCLVSIVH HDFKETDELW

EMLLDQA
Length:657
Mass (Da):73,942
Last modified:October 1, 1996 - v2
Checksum:iD75612EA92D67500
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti110 – 111DP → NL in K02070 (PubMed:6095044).Curated2
Sequence conflicti115 – 116ED → VV in K02070 (PubMed:6095044).Curated2
Sequence conflicti119D → V in K02070 (PubMed:6095044).Curated1
Sequence conflicti124 – 131ESPFKYAV → RLLNMRLF in K02070 (PubMed:6095044).Curated8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U36624 Genomic DNA. Translation: AAB68164.1.
K02070 Genomic DNA. No translation available.
BK006949 Genomic DNA. Translation: DAA11405.1.
PIRiS63459.
RefSeqiNP_015302.1. NM_001183837.1.

Genome annotation databases

EnsemblFungiiYPL023C; YPL023C; YPL023C.
GeneIDi856084.
KEGGisce:YPL023C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U36624 Genomic DNA. Translation: AAB68164.1.
K02070 Genomic DNA. No translation available.
BK006949 Genomic DNA. Translation: DAA11405.1.
PIRiS63459.
RefSeqiNP_015302.1. NM_001183837.1.

3D structure databases

ProteinModelPortaliP46151.
SMRiP46151.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36154. 51 interactors.
DIPiDIP-2825N.
IntActiP46151. 5 interactors.
MINTiMINT-1699985.

PTM databases

iPTMnetiP46151.

Proteomic databases

MaxQBiP46151.
PRIDEiP46151.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL023C; YPL023C; YPL023C.
GeneIDi856084.
KEGGisce:YPL023C.

Organism-specific databases

EuPathDBiFungiDB:YPL023C.
SGDiS000005944. MET12.

Phylogenomic databases

HOGENOMiHOG000246234.
InParanoidiP46151.
KOiK00297.
OMAiNVTWGAG.
OrthoDBiEOG092C12V2.

Enzyme and pathway databases

UniPathwayiUPA00193.
BioCyciYEAST:YPL023C-MONOMER.

Miscellaneous databases

PROiP46151.

Family and domain databases

CDDicd00537. MTHFR. 1 hit.
Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR004621. Fadh2_euk.
IPR003171. Mehydrof_redctse.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00677. fadh2_euk. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMTHR1_YEAST
AccessioniPrimary (citable) accession number: P46151
Secondary accession number(s): D6W3Y9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 144 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.