Reviewed, UniProtKB/Swiss-Prot P46133 (ABGT_ECOLI)

Names and origin

Protein names

Recommended name:
    Aminobenzoyl-glutamate transport protein

Gene names

Name:	abgT
Synonyms:	ydaH
Ordered Locus Names:	b1336, JW5822
ORF Names:	ECK1332

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	508 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Essential for aminobenzoyl-glutamate utilization. May transport aminobenzoyl-glutamate into the cell. Seems also to increase the sensitivity to low levels of aminobenzoyl-glutamate. Sufficient to confer aminobenzoyl-glutamate utilization phenotype.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Miscellaneous	It is suggested that in wild-type strain abgT would normally be cryptic or not expressed under conditions of growth on minimal medium. Altering the expression of abgT from its wild-type context appears to be the mechanism for obtaining the growth phenotype.

Ontologies

Keywords
Biological process	Amino-acid transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	amino acid transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 508	508	Aminobenzoyl-glutamate transport protein		PRO_0000064422
Regions
	Transmembrane	30 – 50	21	Potential
	Transmembrane	85 – 105	21	Potential
	Transmembrane	121 – 139	19	Potential
	Transmembrane	140 – 159	20	Potential
	Transmembrane	164 – 184	21	Potential
	Transmembrane	211 – 231	21	Potential
	Transmembrane	261 – 281	21	Potential
	Transmembrane	303 – 323	21	Potential
	Transmembrane	343 – 363	21	Potential
	Transmembrane	382 – 402	21	Potential
	Transmembrane	405 – 425	21	Potential
	Transmembrane	439 – 459	21	Potential
	Transmembrane	479 – 499	21	Potential
Experimental info
	Sequence conflict	504	1	Y → S Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P46133-1 [UniParc]. Last modified January 15, 2008. Version 3. Checksum: C33CBCBE7A39E951	FASTA	508	54,886
	10 20 30 40 50 60 MSMSSIPSSS QSGKLYGWVE RIGNKVPHPF LLFIYLIIVL MVTTAILSAF GVSAKNPTDG 70 80 90 100 110 120 TPVVVKNLLS VEGLHWFLPN VIKNFSGFAP LGAILALVLG AGLAERVGLL PALMVKMASH 130 140 150 160 170 180 VNARYASYMV LFIAFFSHIS SDAALVIMPP MGALIFLAVG RHPVAGLLAA IAGVGCGFTA 190 200 210 220 230 240 NLLIVTTDVL LSGISTEAAA AFNPQMHVSV IDNWYFMASS VVVLTIVGGL ITDKIIEPRL 250 260 270 280 290 300 GQWQGNSDEK LQTLTESQRF GLRIAGVVSL LFIAAIALMV IPQNGILRDP INHTVMPSPF 310 320 330 340 350 360 IKGIVPLIIL FFFVVSLAYG IATRTIRRQA DLPHLMIEPM KEMAGFIVMV FPLAQFVAMF 370 380 390 400 410 420 NWSNMGKFIA VGLTDILESS GLSGIPAFVG LALLSSFLCM FIASGSAIWS ILAPIFVPMF 430 440 450 460 470 480 MLLGFHPAFA QILFRIADSS VLPLAPVSPF VPLFLGFLQR YKPDAKLGTY YSLVLPYPLI 490 500 FLVVWLLMLL AWYLVGLPIG PGIYPRLS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of mutations that allow p-aminobenzoyl-glutamate utilization by Escherichia coli." Hussein M.J., Green J.M., Nichols B.P. J. Bacteriol. 180:6260-6268(1998) [PubMed: 9829935] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION. Strain: BN1001 and BN1003.
[2]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:363-377(1996) [PubMed: 9097039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 504. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Characterisation and nucleotide sequence of ogt, the O6-alkylguanine-DNA-alkyltransferase gene of E. coli." Potter P.M., Wilkinson M.C., Fitton J., Carr F.J., Brennand J., Cooper D.P., Margison G.P. Nucleic Acids Res. 15:9177-9193(1987) [PubMed: 2825131] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 452-508.
[6]	"Detection of new genes in a bacterial genome using Markov models for three gene classes." Borodovsky M., McIninch J., Koonin E.V., Rudd K.E., Medigue C., Danchin A. Nucleic Acids Res. 23:3554-3562(1995) [PubMed: 7567469] [Abstract] Cited for: IDENTIFICATION.
[7]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U00096 Genomic DNA. Translation: AAC74418.2. AP009048 Genomic DNA. Translation: BAA14929.2. Y00495 Genomic DNA. No translation available.
PIR	C64883.
RefSeq	AP_001964.1. NP_415852.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:9031N.
STRING	P46133.
Protein family/group databases
TCDB	2.A.68.1.1. p-aminobenzoyl-glutamate transporter (AbgT) family.
Genome annotation databases
GeneID	945912.
GenomeReviews	Gene locus JW5822 in contig AP009048_GR. Gene locus b1336 in contig U00096_GR.
KEGG	ecj:JW5822. eco:b1336.
Organism-specific databases
EchoBASE	EB2697.
EcoGene	EG12853. abgT.
CMR	Search...
Phylogenomic databases
HOGENOM	P46133.
OMA	KWSDKFL
Enzyme and pathway databases
BioCyc	EcoCyc:ABGT-MON. ECOL168927:B1336-MON.
Gene expression databases
Genevestigator	P46133.
Family and domain databases
InterPro	IPR004697. Put_transpt_AbgT. IPR011540. YdaH_transpt. [Graphical view]
Pfam	PF03806. ABG_transport. 1 hit. [Graphical view]
TIGRFAMs	TIGR00819. ydaH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ABGT_ECOLI

Accession

Primary (citable) accession number: P46133
Secondary accession number(s): P76051, P76843, P76845

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	January 15, 2008
Last modified:	November 24, 2009
This is version 78 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)