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Protein

Putative acyl-CoA thioester hydrolase YbhC

Gene

ybhC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Putative thioesterase. Does not bind pectin, and has no pectinesterase activity.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei285NucleophilePROSITE-ProRule annotation1
Binding sitei345SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aspartyl esterase, Hydrolase

Enzyme and pathway databases

BioCyciEcoCyc:EG12875-MONOMER.
ECOL316407:JW0755-MONOMER.
BRENDAi3.1.2.2. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative acyl-CoA thioester hydrolase YbhC (EC:3.1.2.-)
Gene namesi
Name:ybhC
Ordered Locus Names:b0772, JW0755
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12875. ybhC.

Subcellular locationi

GO - Cellular componenti

  • cell outer membrane Source: EcoCyc
  • cell wall Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21By similarityAdd BLAST21
ChainiPRO_000002350222 – 427Putative acyl-CoA thioester hydrolase YbhCAdd BLAST406

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi22N-palmitoyl cysteineCurated1
Lipidationi22S-diacylglycerol cysteineCurated1
Disulfide bondi185 ↔ 1971 Publication

Keywords - PTMi

Disulfide bond, Lipoprotein, Palmitate

Proteomic databases

PaxDbiP46130.
PRIDEiP46130.

Interactioni

Protein-protein interaction databases

BioGridi4261839. 24 interactors.
DIPiDIP-11407N.
IntActiP46130. 3 interactors.
MINTiMINT-1240146.
STRINGi511145.b0772.

Structurei

Secondary structure

1427
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi42 – 44Combined sources3
Helixi47 – 50Combined sources4
Helixi55 – 58Combined sources4
Beta strandi62 – 65Combined sources4
Beta strandi81 – 84Combined sources4
Beta strandi94 – 96Combined sources3
Helixi97 – 105Combined sources9
Beta strandi113 – 117Combined sources5
Beta strandi119 – 124Combined sources6
Beta strandi126 – 128Combined sources3
Beta strandi135 – 139Combined sources5
Beta strandi141 – 143Combined sources3
Helixi144 – 146Combined sources3
Beta strandi147 – 151Combined sources5
Helixi159 – 166Combined sources8
Helixi168 – 170Combined sources3
Helixi179 – 186Combined sources8
Beta strandi190 – 192Combined sources3
Helixi195 – 197Combined sources3
Beta strandi200 – 203Combined sources4
Beta strandi209 – 217Combined sources9
Helixi220 – 222Combined sources3
Beta strandi232 – 235Combined sources4
Beta strandi238 – 248Combined sources11
Beta strandi254 – 256Combined sources3
Beta strandi272 – 277Combined sources6
Beta strandi279 – 297Combined sources19
Beta strandi299 – 302Combined sources4
Beta strandi312 – 316Combined sources5
Beta strandi326 – 330Combined sources5
Beta strandi332 – 335Combined sources4
Beta strandi337 – 339Combined sources3
Beta strandi341 – 347Combined sources7
Beta strandi356 – 361Combined sources6
Beta strandi374 – 378Combined sources5
Beta strandi390 – 392Combined sources3
Turni393 – 395Combined sources3
Beta strandi396 – 398Combined sources3
Beta strandi408 – 413Combined sources6
Helixi417 – 419Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3GRHX-ray1.70A29-427[»]
ProteinModelPortaliP46130.
SMRiP46130.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP46130.

Family & Domainsi

Sequence similaritiesi

Belongs to the pectinesterase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105NBR. Bacteria.
COG4677. LUCA.
HOGENOMiHOG000118057.
InParanoidiP46130.
KOiK01051.
OMAiFNRMWEY.
PhylomeDBiP46130.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
IPR033131. Pectinesterase_Asp_AS.
IPR000070. Pectinesterase_cat.
[Graphical view]
PfamiPF01095. Pectinesterase. 1 hit.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 3 hits.
PROSITEiPS00800. PECTINESTERASE_1. 1 hit.
PS00503. PECTINESTERASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P46130-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTFSVSRLA LALAFGVTLT ACSSTPPDQR PSDQTAPGTS SRPILSAKEA
60 70 80 90 100
QNFDAQHYFA SLTPGAAAWN PSPITLPAQP DFVVGPAGTQ GVTHTTIQAA
110 120 130 140 150
VDAAIIKRTN KRQYIAVMPG EYQGTVYVPA APGGITLYGT GEKPIDVKIG
160 170 180 190 200
LSLDGGMSPA DWRHDVNPRG KYMPGKPAWY MYDSCQSKRS DSIGVLCSAV
210 220 230 240 250
FWSQNNGLQL QNLTIENTLG DSVDAGNHPA VALRTDGDQV QINNVNILGR
260 270 280 290 300
QNTFFVTNSG VQNRLETNRQ PRTLVTNSYI EGDVDIVSGR GAVVFDNTEF
310 320 330 340 350
RVVNSRTQQE AYVFAPATLS NIYYGFLAVN SRFNAFGDGV AQLGRSLDVD
360 370 380 390 400
ANTNGQVVIR DSAINEGFNT AKPWADAVIS NRPFAGNTGS VDDNDEIQRN
410 420
LNDTNYNRMW EYNNRGVGSK VVAEAKK
Length:427
Mass (Da):46,082
Last modified:November 1, 1997 - v2
Checksum:i208831BE0EDBCB5C
GO

Sequence cautioni

The sequence U39938 differs from that shown. Reason: Frameshift at position 37.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti167N → Q (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73859.1.
AP009048 Genomic DNA. Translation: BAA35436.2.
U39938 Genomic DNA. No translation available.
J01638 Genomic DNA. No translation available.
PIRiD64813.
RefSeqiNP_415293.1. NC_000913.3.
WP_001091569.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73859; AAC73859; b0772.
BAA35436; BAA35436; BAA35436.
GeneIDi945381.
KEGGiecj:JW0755.
eco:b0772.
PATRICi32116745. VBIEscCol129921_0798.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73859.1.
AP009048 Genomic DNA. Translation: BAA35436.2.
U39938 Genomic DNA. No translation available.
J01638 Genomic DNA. No translation available.
PIRiD64813.
RefSeqiNP_415293.1. NC_000913.3.
WP_001091569.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3GRHX-ray1.70A29-427[»]
ProteinModelPortaliP46130.
SMRiP46130.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261839. 24 interactors.
DIPiDIP-11407N.
IntActiP46130. 3 interactors.
MINTiMINT-1240146.
STRINGi511145.b0772.

Proteomic databases

PaxDbiP46130.
PRIDEiP46130.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73859; AAC73859; b0772.
BAA35436; BAA35436; BAA35436.
GeneIDi945381.
KEGGiecj:JW0755.
eco:b0772.
PATRICi32116745. VBIEscCol129921_0798.

Organism-specific databases

EchoBASEiEB2713.
EcoGeneiEG12875. ybhC.

Phylogenomic databases

eggNOGiENOG4105NBR. Bacteria.
COG4677. LUCA.
HOGENOMiHOG000118057.
InParanoidiP46130.
KOiK01051.
OMAiFNRMWEY.
PhylomeDBiP46130.

Enzyme and pathway databases

BioCyciEcoCyc:EG12875-MONOMER.
ECOL316407:JW0755-MONOMER.
BRENDAi3.1.2.2. 2026.

Miscellaneous databases

EvolutionaryTraceiP46130.
PROiP46130.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
IPR033131. Pectinesterase_Asp_AS.
IPR000070. Pectinesterase_cat.
[Graphical view]
PfamiPF01095. Pectinesterase. 1 hit.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 3 hits.
PROSITEiPS00800. PECTINESTERASE_1. 1 hit.
PS00503. PECTINESTERASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiYBHC_ECOLI
AccessioniPrimary (citable) accession number: P46130
Secondary accession number(s): P75765
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Lacks the conserved Asp residue in position 252 essential for pectinase activity. Likewise, most of the residues involved in substrate binding are not conserved. Was originally (PubMed:15808744) thought to have palmitoyl-CoA thioesterase activity, but PubMed:19452549 were unable to detect any pectinase or palmitoyl-CoA thioesterase activity. Its enzyme activity is therefore unsure.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.