P46112 (KAX33_LEIQH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Potassium channel toxin alpha-KTx 3.3 Alternative name(s): Agitoxin-3 Short name=AgTx-3 Short name=AgTx3
Organism	Leiurus quinquestriatus hebraeus (Yellow scorpion)
Taxonomic identifier	6884 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Chelicerata › Arachnida › Scorpiones › Buthida › Buthoidea › Buthidae › Leiurus › Leiurus quinquestriatus

Protein attributes

Sequence length	38 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Potent inhibitor of shaker potassium channels as well as the mammalian homologs of shaker.
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Sequence similarities	Belongs to the short scorpion toxin superfamily. Potassium channel inhibitor family. Alpha-KTx 3 subfamily.

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Ion channel impairing toxin Neurotoxin Potassium channel inhibitor Toxin
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	pathogenesis Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	potassium channel inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Peptide	1 – 38	38	Potassium channel toxin alpha-KTx 3.3		PRO_0000044927
Regions
	Region	26 – 33	8	Interaction with Ca(2+)-activated K(+) channels Potential
Amino acid modifications
	Disulfide bond	8 ↔ 28		By similarity
	Disulfide bond	14 ↔ 33		By similarity
	Disulfide bond	18 ↔ 35		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P46112 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: E5945513F87B51CE	FASTA	38	4,107
	10 20 30 GVPINVPCTG SPQCIKPCKD AGMRFGKCMN RKCHCTPK

References

[1]

"Purification and characterization of three inhibitors of voltage-dependent K+ channels from Leiurus quinquestriatus var. hebraeus venom." Garcia M.L., Garcia-Calvo M., Hidalgo P., Lee A., Mackinnon R. Biochemistry 33:6834-6839(1994) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Venom.

[2]

"Moving pieces in a taxonomic puzzle: venom 2D-LC/MS and data clustering analyses to infer phylogenetic relationships in some scorpions from the Buthidae family (Scorpiones)." Nascimento D.G., Rates B., Santos D.M., Verano-Braga T., Barbosa-Silva A., Dutra A.A.A., Biondi I., Martin-Eauclaire M.-F., De Lima M.E., Pimenta A.M.C. Toxicon 47:628-639(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY.

Cross-references

Sequence databases
PIR	C54471.
3D structure databases
ProteinModelPortal	P46112.
SMR	P46112. Positions 1-38.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.30.30.10. 1 hit.
InterPro	IPR003614. Scorpion_toxin-like. IPR001947. Scorpion_toxinS_K_inh. [Graphical view]
Pfam	PF00451. Toxin_2. 1 hit. [Graphical view]
PRINTS	PR00286. CHARYBDTOXIN.
ProDom	PD003586. Scorpion_toxinS. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF57095. SSF57095. 1 hit.
PROSITE	PS01138. SCORP_SHORT_TOXIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KAX33_LEIQH

Accession

Primary (citable) accession number: P46112

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Animal Toxin Annotation Program