P46111 (KAX32_LEIQH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Potassium channel toxin alpha-KTx 3.2 Alternative name(s): Agitoxin-2 Short name=AgTx-2 Short name=AgTx2
Organism	Leiurus quinquestriatus hebraeus (Yellow scorpion)
Taxonomic identifier	6884 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Chelicerata › Arachnida › Scorpiones › Buthida › Buthoidea › Buthidae › Leiurus › Leiurus quinquestriatus

Protein attributes

Sequence length	38 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Potent inhibitor of shaker potassium channels as well as the mammalian homologs of shaker.
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Domain	Has the structural arrangement of an alpha-helix connected to a beta-sheet by disulfide bonds (CSalpha/beta).
Sequence similarities	Belongs to the short scorpion toxin superfamily. Potassium channel inhibitor family. Alpha-KTx 3 subfamily.

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Ion channel impairing toxin Neurotoxin Potassium channel inhibitor Toxin
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	pathogenesis Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	potassium channel inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Peptide

1 – 38

38

Potassium channel toxin alpha-KTx 3.2

PRO_0000044926

Regions

Region

26 – 33

8

Interaction with Ca(2+)-activated K(+) channels Potential

Amino acid modifications

Disulfide bond

8 ↔ 28

Ref.2

Disulfide bond

14 ↔ 33

Ref.2

Disulfide bond

18 ↔ 35

Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

38

Helix Strand Turn

Details...

Helix

11 – 14

4

Helix

15 – 21

7

Beta strand

23 – 29

7

Beta strand

32 – 37

6

Sequences

	Sequence		Length	Mass (Da)
	P46111 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: C8BB8513F87B51CD	FASTA	38	4,097
	10 20 30 GVPINVSCTG SPQCIKPCKD AGMRFGKCMN RKCHCTPK

References

[1]	"Purification and characterization of three inhibitors of voltage-dependent K+ channels from Leiurus quinquestriatus var. hebraeus venom." Garcia M.L., Garcia-Calvo M., Hidalgo P., Lee A., Mackinnon R. Biochemistry 33:6834-6839(1994) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Venom.
[2]	"Solution structure of the potassium channel inhibitor agitoxin 2: caliper for probing channel geometry." Krezel A.M., Kasibhatla C., Hidalgo P., Mackinnon R., Wagner G. Protein Sci. 4:1478-1489(1995) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR, DISULFIDE BONDS.

Cross-references

Sequence databases

PIR

B54471.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1AGT	NMR	-	A	1-38	[»]

ProteinModelPortal

P46111.

SMR

P46111. Positions 1-38.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

3.30.30.10. 1 hit.

InterPro

IPR003614. Scorpion_toxin-like.
IPR001947. Scorpion_toxinS_K_inh.
[Graphical view]

Pfam

PF00451. Toxin_2. 1 hit.
[Graphical view]

PRINTS

PR00286. CHARYBDTOXIN.

ProDom

PD003586. Scorpion_toxinS. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SUPFAM

SSF57095. SSF57095. 1 hit.

PROSITE

PS01138. SCORP_SHORT_TOXIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P46111.

Entry information

Entry name

KAX32_LEIQH

Accession

Primary (citable) accession number: P46111

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Animal Toxin Annotation Program