Reviewed,
UniProtKB/Swiss-Prot P46109 (CRKL_HUMAN)
Last modified
November 4, 2008.
Version 81.
History...
Clusters with 100%,
90%,
50% identity |
Documents (4) |
Third-party data |
Customize display | text xml rdf/xml gff fasta |
Names and origin
| Protein names | Recommended name: Crk-like protein | ||
| Gene names |
| ||
| Organism | Homo sapiens (Human) | ||
| Taxonomic identifier | 9606 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 303 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | May mediate the transduction of intracellular signals. |
| Subunit structure | Interacts with INPP5D/SHIP1. Interacts with DOCK2 and EPOR. Interacts with phosphorylated CBLB and IRS4. |
| Sequence similarities | Contains 1 SH2 domain. Contains 2 SH3 domains. |
Ontologies
Keywords | |
|---|---|
| Domain | Repeat SH2 domain SH3 domain |
| PTM | Phosphoprotein |
| Technical term | 3D-structure |
Gene Ontology (GO) | |
| Biological process | JNK cascade Traceable author statement. Source: ProtInc Ras protein signal transductionTraceable author statement. Source: ProtInc |
| Cellular component | cytoplasm Inferred from direct assay. Source: HPA plasma membraneInferred from direct assay. Source: HPA |
| Molecular function | SH3/SH2 adaptor activity Traceable author statement. Source: ProtInc protein tyrosine kinase activity Ref.1Traceable author statement. Source: ProtInc |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| Arms | Q9EQG6 | 1 | EBI-910,EBI-976654 | From a different organism. |
| MAP4K1 | Q92918 | 3 | EBI-910,EBI-881 | |
| MAP4K5 | Q9Y4K4 | 1 | EBI-910,EBI-1279 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||||||||
Molecule processing | |||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 303 | 303 | Crk-like protein | PRO_0000079347 | |||||||||||||
Regions | |||||||||||||||||
| Domain | 14 – 102 | 89 | SH2 | ||||||||||||||
| Domain | 123 – 183 | 61 | SH3 1 | ||||||||||||||
| Domain | 235 – 296 | 62 | SH3 2 | ||||||||||||||
Amino acid modifications | |||||||||||||||||
| Modified residue | 107 | 1 | Phosphoserine By similarity | ||||||||||||||
| Modified residue | 132 | 1 | Phosphotyrosine By similarity | ||||||||||||||
| Modified residue | 207 | 1 | Phosphotyrosine | ||||||||||||||
| Modified residue | 251 | 1 | Phosphotyrosine | ||||||||||||||
Secondary structure | |||||||||||||||||
Helix Strand Turn | |||||||||||||||||
| Beta strand | 240 – 242 | 3 | |||||||||||||||
| Beta strand | 271 – 276 | 6 | |||||||||||||||
| Beta strand | 285 – 287 | 3 | |||||||||||||||
| Helix | 288 – 290 | 3 | |||||||||||||||
| Beta strand | 291 – 293 | 3 | |||||||||||||||
Sequences
| ||||||||||||||||||
References
| « Hide 'large scale' references | |
| [1] | "Isolation and chromosomal localization of CRKL, a human crk-like gene." ten Hoeve J., Morris C., Heisterkamp N., Groffen J. Oncogene 8:2469-2474(1993) [PubMed: 8361759] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Spleen. |
| [2] | "A genome annotation-driven approach to cloning the human ORFeome." Collins J.E., Wright C.L., Edwards C.A., Davis M.P., Grinham J.A., Cole C.G., Goward M.E., Aguado B., Mallya M., Mokrab Y., Huckle E.J., Beare D.M., Dunham I. Genome Biol. 5:RESEARCH84.1-RESEARCH84.11(2004) [PubMed: 15461802] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [3] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skin. |
| [4] | "Interplay of the proto-oncogene proteins CrkL and CrkII in insulin-like growth factor-I receptor-mediated signal transduction." Koval A.P., Karas M., Zick Y., LeRoith D. J. Biol. Chem. 273:14780-14787(1998) [PubMed: 9614078] [Abstract] Cited for: INTERACTION WITH IRS4. |
| [5] | "Tyrosine phosphorylation and complex formation of Cbl-b upon T cell receptor stimulation." Elly C., Witte S., Zhang Z., Rosnet O., Lipkowitz S., Altman A., Liu Y.-C. Oncogene 18:1147-1156(1999) [PubMed: 10022120] [Abstract] Cited for: INTERACTION WITH CBLB. |
| [6] | "DOCK2 associates with CrkL and regulates Rac1 in human leukemia cell lines." Nishihara H., Maeda M., Oda A., Tsuda M., Sawa H., Nagashima K., Tanaka S. Blood 100:3968-3974(2002) [PubMed: 12393632] [Abstract] Cited for: INTERACTION WITH DOCK2. |
| [7] | "CrkL is recruited through its SH2 domain to the erythropoietin receptor and plays a role in Lyn-mediated receptor signaling." Arai A., Kanda E., Nosaka Y., Miyasaka N., Miura O. J. Biol. Chem. 276:33282-33290(2001) [PubMed: 11443118] [Abstract] Cited for: INTERACTION WITH EPOR. |
| [8] | "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J. Nat. Biotechnol. 23:94-101(2005) [PubMed: 15592455] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-207 AND TYR-251, MASS SPECTROMETRY. |
| [9] | "Solution structures of the SH3 domain of human CRK-like protein." RIKEN structural genomics initiative (RSGI) Submitted (DEC-2006) to the PDB data bank Cited for: STRUCTURE BY NMR OF 220-303. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| X59656 mRNA. Translation: CAA42199.1. CR456423 mRNA. Translation: CAG30309.1. BC043500 mRNA. Translation: AAH43500.1. | |||||||||||||||||||||||||||||||
| PIR | S41754. | ||||||||||||||||||||||||||||||
| RefSeq | NP_005198.1. | ||||||||||||||||||||||||||||||
| UniGene | Hs.592205 | ||||||||||||||||||||||||||||||
3D structure databases | |||||||||||||||||||||||||||||||
| |||||||||||||||||||||||||||||||
| ModBase | Search... | ||||||||||||||||||||||||||||||
Protein-protein interaction databases | |||||||||||||||||||||||||||||||
| DIP | DIP:29165N. | ||||||||||||||||||||||||||||||
| IntAct | P46109. | ||||||||||||||||||||||||||||||
PTM databases | |||||||||||||||||||||||||||||||
| PhosphoSite | P46109. | ||||||||||||||||||||||||||||||
2-D gel databases | |||||||||||||||||||||||||||||||
| OGP | P46109. | ||||||||||||||||||||||||||||||
Proteomic databases | |||||||||||||||||||||||||||||||
| PeptideAtlas | P46109. | ||||||||||||||||||||||||||||||
Genome annotation databases | |||||||||||||||||||||||||||||||
| Ensembl | ENSG00000099942. Homo sapiens. [Contig view] | ||||||||||||||||||||||||||||||
| GeneID | 1399. | ||||||||||||||||||||||||||||||
| KEGG | hsa:1399. | ||||||||||||||||||||||||||||||
Organism-specific databases | |||||||||||||||||||||||||||||||
| H-InvDB | HIX0016264. | ||||||||||||||||||||||||||||||
| HGNC | HGNC:2363. CRKL. | ||||||||||||||||||||||||||||||
| HPA | HPA001100. | ||||||||||||||||||||||||||||||
| MIM | 602007. gene. | ||||||||||||||||||||||||||||||
| PharmGKB | PA26881. | ||||||||||||||||||||||||||||||
| GenAtlas | Search... | ||||||||||||||||||||||||||||||
| GeneCards | Search... | ||||||||||||||||||||||||||||||
Phylogenomic databases | |||||||||||||||||||||||||||||||
| HOGENOM | P46109. | ||||||||||||||||||||||||||||||
| HOVERGEN | P46109. | ||||||||||||||||||||||||||||||
Gene expression databases | |||||||||||||||||||||||||||||||
| ArrayExpress | P46109. | ||||||||||||||||||||||||||||||
| CleanEx | HS_CRKL. | ||||||||||||||||||||||||||||||
| GermOnline | ENSG00000099942. Homo sapiens. | ||||||||||||||||||||||||||||||
Family and domain databases | |||||||||||||||||||||||||||||||
| InterPro | IPR000980. SH2. IPR001452. SH3. IPR011511. SH3_2. [Graphical view] | ||||||||||||||||||||||||||||||
| Gene3D | G3DSA:3.30.505.10. SH2. 1 hit. | ||||||||||||||||||||||||||||||
| Pfam | PF00017. SH2. 1 hit. PF00018. SH3_1. 1 hit. PF07653. SH3_2. 1 hit. [Graphical view] | ||||||||||||||||||||||||||||||
| PRINTS | PR00401. SH2DOMAIN. PR00452. SH3DOMAIN. | ||||||||||||||||||||||||||||||
| ProDom | PD000093. SH2. 1 hit. PD000066. SH3. 2 hits. [Graphical view] [Entries sharing at least one domain] | ||||||||||||||||||||||||||||||
| SMART | SM00252. SH2. 1 hit. SM00326. SH3. 2 hits. [Graphical view] | ||||||||||||||||||||||||||||||
| PROSITE | PS50001. SH2. 1 hit. PS50002. SH3. 1 hit. [Graphical view] | ||||||||||||||||||||||||||||||
| ProtoNet | Search... | ||||||||||||||||||||||||||||||
Other Resources | |||||||||||||||||||||||||||||||
| LinkHub | P46109. | ||||||||||||||||||||||||||||||
| NextBio | 5727. | ||||||||||||||||||||||||||||||
| SOURCE | Search... | ||||||||||||||||||||||||||||||
Entry information
| Entry name | CRKL_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P46109 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 22 Human chromosome 22: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with


