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P46109 (CRKL_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 141. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Crk-like protein
Gene names
Name:CRKL
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length303 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May mediate the transduction of intracellular signals.

Subunit structure

Interacts with INPP5D/SHIP1. Interacts with DOCK2 and EPOR. Interacts with phosphorylated CBLB and IRS4. Ref.6 Ref.7 Ref.8 Ref.9

Sequence similarities

Belongs to the CRK family.

Contains 1 SH2 domain.

Contains 2 SH3 domains.

Ontologies

Keywords
   DomainRepeat
SH2 domain
SH3 domain
   PTMPhosphoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processJNK cascade

Traceable author statement PubMed 8798523. Source: ProtInc

Ras protein signal transduction

Traceable author statement PubMed 8798523. Source: ProtInc

activation of MAPKK activity

Traceable author statement. Source: Reactome

anterior/posterior pattern specification

Inferred from electronic annotation. Source: Ensembl

blood vessel development

Inferred from electronic annotation. Source: Ensembl

heart development

Inferred from electronic annotation. Source: Ensembl

intracellular signal transduction

Traceable author statement Ref.1. Source: ProtInc

neurotrophin TRK receptor signaling pathway

Traceable author statement. Source: Reactome

organ morphogenesis

Inferred from electronic annotation. Source: Ensembl

parathyroid gland development

Inferred from electronic annotation. Source: Ensembl

positive regulation of signal transduction

Traceable author statement PubMed 8798523. Source: GOC

thymus development

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcytosol

Traceable author statement. Source: Reactome

endosome

Traceable author statement. Source: Reactome

   Molecular_functionSH3/SH2 adaptor activity

Traceable author statement PubMed 8798523. Source: ProtInc

poly(A) RNA binding

Inferred from direct assay PubMed 22658674. Source: UniProtKB

signal transducer activity

Traceable author statement Ref.1PubMed 8798523. Source: ProtInc

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 303303Crk-like protein
PRO_0000079347

Regions

Domain14 – 10289SH2
Domain123 – 18361SH3 1
Domain235 – 29662SH3 2

Amino acid modifications

Modified residue1271Phosphotyrosine Ref.12
Modified residue2071Phosphotyrosine Ref.10

Secondary structure

........................................................ 303
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P46109 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 294CF1EE2CD44B81

FASTA30333,777
        10         20         30         40         50         60 
MSSARFDSSD RSAWYMGPVS RQEAQTRLQG QRHGMFLVRD SSTCPGDYVL SVSENSRVSH 

        70         80         90        100        110        120 
YIINSLPNRR FKIGDQEFDH LPALLEFYKI HYLDTTTLIE PAPRYPSPPM GSVSAPNLPT 

       130        140        150        160        170        180 
AEDNLEYVRT LYDFPGNDAE DLPFKKGEIL VIIEKPEEQW WSARNKDGRV GMIPVPYVEK 

       190        200        210        220        230        240 
LVRSSPHGKH GNRNSNSYGI PEPAHAYAQP QTTTPLPAVS GSPGAAITPL PSTQNGPVFA 

       250        260        270        280        290        300 
KAIQKRVPCA YDKTALALEV GDIVKVTRMN INGQWEGEVN GRKGLFPFTH VKIFDPQNPD 


ENE 

« Hide

References

« Hide 'large scale' references
[1]"Isolation and chromosomal localization of CRKL, a human crk-like gene."
ten Hoeve J., Morris C., Heisterkamp N., Groffen J.
Oncogene 8:2469-2474(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Spleen.
[2]"A genome annotation-driven approach to cloning the human ORFeome."
Collins J.E., Wright C.L., Edwards C.A., Davis M.P., Grinham J.A., Cole C.G., Goward M.E., Aguado B., Mallya M., Mokrab Y., Huckle E.J., Beare D.M., Dunham I.
Genome Biol. 5:R84.1-R84.11(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Trachea.
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Skin.
[6]"Interplay of the proto-oncogene proteins CrkL and CrkII in insulin-like growth factor-I receptor-mediated signal transduction."
Koval A.P., Karas M., Zick Y., LeRoith D.
J. Biol. Chem. 273:14780-14787(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH IRS4.
[7]"Tyrosine phosphorylation and complex formation of Cbl-b upon T cell receptor stimulation."
Elly C., Witte S., Zhang Z., Rosnet O., Lipkowitz S., Altman A., Liu Y.-C.
Oncogene 18:1147-1156(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CBLB.
[8]"DOCK2 associates with CrkL and regulates Rac1 in human leukemia cell lines."
Nishihara H., Maeda M., Oda A., Tsuda M., Sawa H., Nagashima K., Tanaka S.
Blood 100:3968-3974(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH DOCK2.
[9]"CrkL is recruited through its SH2 domain to the erythropoietin receptor and plays a role in Lyn-mediated receptor signaling."
Arai A., Kanda E., Nosaka Y., Miyasaka N., Miura O.
J. Biol. Chem. 276:33282-33290(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH EPOR.
[10]"Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-207, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[12]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-127, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[13]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[14]"Solution structures of the SH3 domain of human CRK-like protein."
RIKEN structural genomics initiative (RSGI)
Submitted (DEC-2006) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 220-303.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X59656 mRNA. Translation: CAA42199.1.
CR456423 mRNA. Translation: CAG30309.1.
AK292909 mRNA. Translation: BAF85598.1.
CH471176 Genomic DNA. Translation: EAX02932.1.
CH471176 Genomic DNA. Translation: EAX02933.1.
CH471176 Genomic DNA. Translation: EAX02934.1.
BC043500 mRNA. Translation: AAH43500.1.
PIRS41754.
RefSeqNP_005198.1. NM_005207.3.
UniGeneHs.5613.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2BZXX-ray2.80A237-303[»]
2BZYX-ray2.50A/B237-303[»]
2DBKNMR-A229-303[»]
2EO3NMR-A1-104[»]
2LQNNMR-A1-303[»]
2LQWNMR-A1-303[»]
ProteinModelPortalP46109.
SMRP46109. Positions 1-303.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid107789. 66 interactions.
DIPDIP-29165N.
IntActP46109. 55 interactions.
MINTMINT-137265.
STRING9606.ENSP00000346300.

PTM databases

PhosphoSiteP46109.

Polymorphism databases

DMDM1169094.

2D gel databases

OGPP46109.

Proteomic databases

PaxDbP46109.
PeptideAtlasP46109.
PRIDEP46109.

Protocols and materials databases

DNASU1399.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000354336; ENSP00000346300; ENSG00000099942.
ENST00000411769; ENSP00000396646; ENSG00000099942.
GeneID1399.
KEGGhsa:1399.
UCSCuc002ztf.2. human.

Organism-specific databases

CTD1399.
GeneCardsGC22P021272.
HGNCHGNC:2363. CRKL.
HPAHPA001100.
MIM602007. gene.
neXtProtNX_P46109.
Orphanet261330. Distal 22q11.2 microdeletion syndrome.
PharmGKBPA26881.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG292767.
HOGENOMHOG000236288.
HOVERGENHBG105616.
InParanoidP46109.
KOK04438.
OMARTLYDFT.
OrthoDBEOG7NW69P.
PhylomeDBP46109.
TreeFamTF321436.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.
REACT_6900. Immune System.
SignaLinkP46109.

Gene expression databases

BgeeP46109.
CleanExHS_CRKL.
GenevestigatorP46109.

Family and domain databases

Gene3D3.30.505.10. 1 hit.
InterProIPR000980. SH2.
IPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]
PfamPF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
PF07653. SH3_2. 1 hit.
[Graphical view]
PRINTSPR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
SMARTSM00252. SH2. 1 hit.
SM00326. SH3. 2 hits.
[Graphical view]
SUPFAMSSF50044. SSF50044. 2 hits.
SSF55550. SSF55550. 2 hits.
PROSITEPS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSCRKL. human.
EvolutionaryTraceP46109.
GeneWikiCRKL.
GenomeRNAi1399.
NextBio5727.
PROP46109.
SOURCESearch...

Entry information

Entry nameCRKL_HUMAN
AccessionPrimary (citable) accession number: P46109
Secondary accession number(s): A8KA44, D3DX35
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 16, 2014
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 22

Human chromosome 22: entries, gene names and cross-references to MIM