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Protein

Pyrrolidone-carboxylate peptidase

Gene

pcp

Organism
Bacillus amyloliquefaciens (Bacillus velezensis)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Removes 5-oxoproline from various penultimate amino acid residues except L-proline.

Catalytic activityi

Release of an N-terminal pyroglutamyl group from a polypeptide, the second amino acid generally not being Pro.

pH dependencei

Optimum pH is 6.5. Stable from pH 7.0 to 9.0.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei811
Active sitei1441
Active sitei1681

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Protein family/group databases

MEROPSiC15.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyrrolidone-carboxylate peptidase (EC:3.4.19.3)
Alternative name(s):
5-oxoprolyl-peptidase
Pyroglutamyl-peptidase I
Short name:
PGP-I
Short name:
Pyrase
Gene namesi
Name:pcp
OrganismiBacillus amyloliquefaciens (Bacillus velezensis)
Taxonomic identifieri1390 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi68C → S: No loss of activity. 1 Publication1
Mutagenesisi144C → S: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001847061 – 215Pyrrolidone-carboxylate peptidaseAdd BLAST215

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

STRINGi326423.RBAM_002960.

Structurei

Secondary structure

1215
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 10Combined sources8
Helixi20 – 27Combined sources8
Turni28 – 30Combined sources3
Beta strandi36 – 43Combined sources8
Helixi49 – 61Combined sources13
Beta strandi64 – 71Combined sources8
Beta strandi77 – 86Combined sources10
Beta strandi111 – 114Combined sources4
Helixi119 – 128Combined sources10
Helixi143 – 158Combined sources16
Beta strandi163 – 169Combined sources7
Helixi173 – 175Combined sources3
Beta strandi177 – 179Combined sources3
Helixi186 – 202Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AUGX-ray2.00A/B/C/D1-215[»]
3RNZX-ray2.01A/B/C/D1-215[»]
3RO0X-ray1.50A/B/C/D1-215[»]
ProteinModelPortaliP46107.
SMRiP46107.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP46107.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C15 family.Curated

Phylogenomic databases

eggNOGiENOG4108KN7. Bacteria.
COG2039. LUCA.

Family and domain databases

CDDicd00501. Peptidase_C15. 1 hit.
Gene3Di3.40.630.20. 1 hit.
HAMAPiMF_00417. Pyrrolid_peptidase. 1 hit.
InterProiIPR000816. Peptidase_C15.
IPR016125. Peptidase_C15-like.
IPR029762. PGP-I_bact-type.
IPR033694. PGPEP1_Cys_AS.
IPR033693. PGPEP1_Glu_AS.
[Graphical view]
PANTHERiPTHR23402. PTHR23402. 1 hit.
PfamiPF01470. Peptidase_C15. 1 hit.
[Graphical view]
PIRSFiPIRSF015592. Prld-crbxl_pptds. 1 hit.
PRINTSiPR00706. PYROGLUPTASE.
TIGRFAMsiTIGR00504. pyro_pdase. 1 hit.
PROSITEiPS01334. PYRASE_CYS. 1 hit.
PS01333. PYRASE_GLU. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P46107-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKKVLLTGF DPFGGETVNP SWEAVKRLNG AAEGPASIVS EQVPTVFYKS
60 70 80 90 100
LAVLREAIKK HQPDIIICVG QAGGRMQITP ERVAINLNEA RIPDNEGNQP
110 120 130 140 150
VGEDISQGGP AAYWTGLPIK RIVEEIKKEG IPAAVSYTAG TFVCNHLFYG
160 170 180 190 200
LMDEISRHHP HIRGGFIHIP YIPEQTLQKS APSLSLDHIT KALKIAAVTA
210
AVHEDDIETG GGELH
Length:215
Mass (Da):23,287
Last modified:November 1, 1995 - v1
Checksum:i731A9F80733E807C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D11035 Genomic DNA. Translation: BAA01791.1.
PIRiJX0244.
RefSeqiWP_013350909.1. NZ_LYUG01000024.1.

Genome annotation databases

GeneIDi9780258.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D11035 Genomic DNA. Translation: BAA01791.1.
PIRiJX0244.
RefSeqiWP_013350909.1. NZ_LYUG01000024.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AUGX-ray2.00A/B/C/D1-215[»]
3RNZX-ray2.01A/B/C/D1-215[»]
3RO0X-ray1.50A/B/C/D1-215[»]
ProteinModelPortaliP46107.
SMRiP46107.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi326423.RBAM_002960.

Protein family/group databases

MEROPSiC15.001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi9780258.

Phylogenomic databases

eggNOGiENOG4108KN7. Bacteria.
COG2039. LUCA.

Miscellaneous databases

EvolutionaryTraceiP46107.

Family and domain databases

CDDicd00501. Peptidase_C15. 1 hit.
Gene3Di3.40.630.20. 1 hit.
HAMAPiMF_00417. Pyrrolid_peptidase. 1 hit.
InterProiIPR000816. Peptidase_C15.
IPR016125. Peptidase_C15-like.
IPR029762. PGP-I_bact-type.
IPR033694. PGPEP1_Cys_AS.
IPR033693. PGPEP1_Glu_AS.
[Graphical view]
PANTHERiPTHR23402. PTHR23402. 1 hit.
PfamiPF01470. Peptidase_C15. 1 hit.
[Graphical view]
PIRSFiPIRSF015592. Prld-crbxl_pptds. 1 hit.
PRINTSiPR00706. PYROGLUPTASE.
TIGRFAMsiTIGR00504. pyro_pdase. 1 hit.
PROSITEiPS01334. PYRASE_CYS. 1 hit.
PS01333. PYRASE_GLU. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPCP_BACAM
AccessioniPrimary (citable) accession number: P46107
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.