Reviewed, UniProtKB/Swiss-Prot P46103 (DRTS_PLAVN)

Names and origin

Protein names	Recommended name:     Bifunctional dihydrofolate reductase-thymidylate synthase       Short name=DHFR-TS Including the following 2 domains:     1- Recommended name:             Dihydrofolate reductase               EC=1.5.1.3     2- Recommended name:             Thymidylate synthase               EC=2.1.1.45
Organism	Plasmodium vinckei
Taxonomic identifier	5860 [NCBI]
Taxonomic lineage	Eukaryota › Alveolata › Apicomplexa › Aconoidasida › Haemosporida › Plasmodium › Plasmodium (Vinckeia)

Protein attributes

Sequence length	182 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH. 5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.
Pathway	Cofactor biosynthesis; tetrahydrofolate biosynthesis; tetrahydrofolate from dihydrofolate: step 1/1.
Miscellaneous	The reaction catalyzed by this enzyme represents an essential step for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP.
Sequence similarities	In the N-terminal section; belongs to the dihydrofolate reductase family. In the C-terminal section; belongs to the thymidylate synthase family. Contains 1 DHFR (dihydrofolate reductase) domain.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis One-carbon metabolism
Ligand	NADP
Molecular function	Methyltransferase Oxidoreductase Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	glycine biosynthetic process Inferred from electronic annotation. Source: InterPro nucleotide biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon compound metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro dihydrofolate reductase activity Inferred from electronic annotation. Source: InterPro thymidylate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›182	›182	Bifunctional dihydrofolate reductase-thymidylate synthase		PRO_0000186350
Regions
	Domain	‹1 – ›182	›182	DHFR
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	182	1

Sequences

	Sequence		Length	Mass (Da)
	P46103-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 33CCD27441763198	FASTA	182	20,925
	10 20 30 40 50 60 AICACCKVLN SNEKASCFSN KTFKGLGNAG GLPWKCNSVD MKHFVSVTSY VNENNYIRLK 70 80 90 100 110 120 WKRDKYIKEN NVKVNTDGIP SIDKLQNIVV MGKTSWESIP SKFKPLENRI NIILSRTLKK 130 140 150 160 170 180 ENLAKEYSNV IIIKSVDELF PILKCIKYYK CFIIGGASVY KEFLDRNLIK KIYFTRINNA YT

References

[1]	"The dihydrofolate reductase domain of rodent malarias: point mutations and pyrimethamine resistance." Cheng Q., Saul A. Mol. Biochem. Parasitol. 65:361-363(1994) [PubMed: 7969277] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	L28121 Genomic DNA. Translation: AAA29582.1.
3D structure databases
HSSP	HSSP built from PDB template 1J3I based on UniProtKB P13922.
ModBase	Search...
Family and domain databases
InterPro	IPR012259. DHFR. IPR001796. DHFR_reg. IPR000398. Thymidylat_synth_C. [Graphical view]
PANTHER	PTHR11549:SF1. DHFR. 1 hit.
Pfam	PF00186. DHFR_1. 1 hit. [Graphical view]
PRINTS	PR00070. DHFR.
PROSITE	PS00075. DHFR_1. 1 hit. PS51330. DHFR_2. 1 hit. PS00091. THYMIDYLATE_SYNTHASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DRTS_PLAVN

Accession

Primary (citable) accession number: P46103

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 25, 2008
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)