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Protein

Synaptotagmin-2

Gene

Syt2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Exhibits calcium-dependent phospholipid and inositol polyphosphate binding properties (PubMed:7961887). May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse (PubMed:7961887). Plays a role in dendrite formation by melanocytes (By similarity).By similarity1 Publication

Cofactori

Ca2+By similarityNote: Binds 3 Ca2+ ions per subunit. The ions are bound to the C2 domains.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi172Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi173Calcium 1By similarity1
Metal bindingi173Calcium 2By similarity1
Metal bindingi179Calcium 1By similarity1
Metal bindingi231Calcium 1By similarity1
Metal bindingi231Calcium 2By similarity1
Metal bindingi232Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi233Calcium 1By similarity1
Metal bindingi233Calcium 2By similarity1
Metal bindingi233Calcium 3By similarity1
Metal bindingi236Calcium 3By similarity1
Metal bindingi237Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi239Calcium 2By similarity1
Metal bindingi239Calcium 3By similarity1

GO - Molecular functioni

  • calcium-dependent phospholipid binding Source: UniProtKB
  • calcium ion binding Source: MGI
  • clathrin binding Source: GO_Central
  • inositol 1,3,4,5 tetrakisphosphate binding Source: UniProtKB
  • phosphatidylserine binding Source: ParkinsonsUK-UCL
  • syntaxin binding Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Differentiation

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptotagmin-2Curated
Alternative name(s):
Inositol polyphosphate-binding protein1 Publication
Short name:
IP4-binding protein1 Publication
Short name:
IP4BP1 Publication
Synaptotagmin IIImported
Short name:
SytIIBy similarity
Gene namesi
Name:Syt2Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:99666. Syt2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 60VesicularSequence analysisAdd BLAST60
Transmembranei61 – 87HelicalSequence analysisAdd BLAST27
Topological domaini88 – 422CytoplasmicSequence analysisAdd BLAST335

GO - Cellular componenti

  • axon Source: MGI
  • cell junction Source: UniProtKB-KW
  • chromaffin granule membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • intracellular membrane-bounded organelle Source: MGI
  • plasma membrane Source: MGI
  • synaptic vesicle membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasm, Cytoplasmic vesicle, Membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001839431 – 422Synaptotagmin-2Add BLAST422

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi32N-linked (GlcNAc...)Sequence analysis1
Modified residuei125PhosphothreonineBy similarity1
Modified residuei128PhosphothreonineCombined sources1
Modified residuei230PhosphotyrosineCombined sources1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP46097.
PaxDbiP46097.
PeptideAtlasiP46097.
PRIDEiP46097.

PTM databases

iPTMnetiP46097.
PhosphoSitePlusiP46097.
SwissPalmiP46097.

Expressioni

Gene expression databases

BgeeiENSMUSG00000026452.
CleanExiMM_SYT2.

Interactioni

Subunit structurei

Homotetramer (Probable). Interacts with SCAMP5 (By similarity). Interacts with STON2 (By similarity).By similarityCurated

Binary interactionsi

WithEntry#Exp.IntActNotes
SCAMP5Q8TAC92EBI-457969,EBI-2695784From a different organism.
Stx1aO355262EBI-457969,EBI-400878

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203612. 3 interactors.
DIPiDIP-32645N.
IntActiP46097. 9 interactors.
MINTiMINT-2411200.
STRINGi10090.ENSMUSP00000112438.

Structurei

Secondary structure

1422
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi46 – 57Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2NP0X-ray2.62B40-60[»]
4KBBX-ray2.30C/D8-61[»]
ProteinModelPortaliP46097.
SMRiP46097.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP46097.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini156 – 245C2 1PROSITE-ProRule annotationAdd BLAST90
Domaini287 – 378C2 2PROSITE-ProRule annotationAdd BLAST92

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni136 – 382Phospholipid bindingCuratedAdd BLAST247

Domaini

The first C2 domain mediates Ca2+-dependent phospholipid binding (PubMed:7961887).
The second C2 domain mediates interaction with Stonin 2. The second C2 domain mediates phospholipid and inositol polyphosphate binding in a calcium-independent manner (PubMed:7961887).1 Publication

Sequence similaritiesi

Belongs to the synaptotagmin family.Curated
Contains 2 C2 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1028. Eukaryota.
ENOG4111B9R. LUCA.
HOGENOMiHOG000232127.
HOVERGENiHBG005010.
InParanoidiP46097.
KOiK19902.
PhylomeDBiP46097.
TreeFamiTF315600.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR001565. Synaptotagmin.
IPR030543. Syt2.
[Graphical view]
PANTHERiPTHR10024:SF223. PTHR10024:SF223. 1 hit.
PfamiPF00168. C2. 2 hits.
[Graphical view]
PRINTSiPR00360. C2DOMAIN.
PR00399. SYNAPTOTAGMN.
SMARTiSM00239. C2. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
PROSITEiPS50004. C2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P46097-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRNIFKRNQE PNVAPATTTA TMPLAPVAPA DNSTESTGPG ESQEDMFAKL
60 70 80 90 100
KEKFFNEINK IPLPPWALIA MAVVAGLLLL TCCFCICKKC CCKKKKNKKE
110 120 130 140 150
KGKGMKNAMN MKDMKGGQDD DDAETGLTEG EGEGEEEKEP ENLGKLQFSL
160 170 180 190 200
DYDFQANQLT VGVLQAAELP ALDMGGTSDP YVKVFLLPDK KKKYETKVHR
210 220 230 240 250
KTLNPAFNET FTFKVPYQEL AGKTLVMAIY DFDRFSKHDI IGEVKVPMNT
260 270 280 290 300
VDLGQPIEEW RDLQGGEKEE PEKLGDICTS LRYVPTAGKL TVCILEAKNL
310 320 330 340 350
KKMDVGGLSD PYVKIHLMQN GKRLKKKKTT VKKKTLNPYF NESFSFEIPF
360 370 380 390 400
EQIQKVQVVV TVLDYDKLGK NEAIGKIFVG SNATGTELRH WSDMLANPRR
410 420
PIAQWHSLKP EEEVDALLGK NK
Length:422
Mass (Da):47,263
Last modified:November 1, 1995 - v1
Checksum:iB4BD13FF70E0481B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti221A → G (PubMed:16141072).Curated1
Sequence conflicti221A → G (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D37793 mRNA. Translation: BAA07041.1.
AF257303 Genomic DNA. Translation: AAF68987.1.
AF257304 mRNA. Translation: AAF68988.1.
AK036357 mRNA. Translation: BAC29397.1.
BC027019 mRNA. Translation: AAH27019.1.
CCDSiCCDS15312.1.
PIRiA55417.
RefSeqiNP_033333.2. NM_009307.3.
XP_006529385.1. XM_006529322.1.
XP_006529386.1. XM_006529323.3.
UniGeneiMm.5102.

Genome annotation databases

GeneIDi20980.
KEGGimmu:20980.
UCSCiuc007csj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D37793 mRNA. Translation: BAA07041.1.
AF257303 Genomic DNA. Translation: AAF68987.1.
AF257304 mRNA. Translation: AAF68988.1.
AK036357 mRNA. Translation: BAC29397.1.
BC027019 mRNA. Translation: AAH27019.1.
CCDSiCCDS15312.1.
PIRiA55417.
RefSeqiNP_033333.2. NM_009307.3.
XP_006529385.1. XM_006529322.1.
XP_006529386.1. XM_006529323.3.
UniGeneiMm.5102.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2NP0X-ray2.62B40-60[»]
4KBBX-ray2.30C/D8-61[»]
ProteinModelPortaliP46097.
SMRiP46097.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203612. 3 interactors.
DIPiDIP-32645N.
IntActiP46097. 9 interactors.
MINTiMINT-2411200.
STRINGi10090.ENSMUSP00000112438.

PTM databases

iPTMnetiP46097.
PhosphoSitePlusiP46097.
SwissPalmiP46097.

Proteomic databases

MaxQBiP46097.
PaxDbiP46097.
PeptideAtlasiP46097.
PRIDEiP46097.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi20980.
KEGGimmu:20980.
UCSCiuc007csj.1. mouse.

Organism-specific databases

CTDi127833.
MGIiMGI:99666. Syt2.

Phylogenomic databases

eggNOGiKOG1028. Eukaryota.
ENOG4111B9R. LUCA.
HOGENOMiHOG000232127.
HOVERGENiHBG005010.
InParanoidiP46097.
KOiK19902.
PhylomeDBiP46097.
TreeFamiTF315600.

Miscellaneous databases

EvolutionaryTraceiP46097.
PROiP46097.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026452.
CleanExiMM_SYT2.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR001565. Synaptotagmin.
IPR030543. Syt2.
[Graphical view]
PANTHERiPTHR10024:SF223. PTHR10024:SF223. 1 hit.
PfamiPF00168. C2. 2 hits.
[Graphical view]
PRINTSiPR00360. C2DOMAIN.
PR00399. SYNAPTOTAGMN.
SMARTiSM00239. C2. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
PROSITEiPS50004. C2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYT2_MOUSE
AccessioniPrimary (citable) accession number: P46097
Secondary accession number(s): Q8R0E1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 144 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.