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P46097

- SYT2_MOUSE

UniProt

P46097 - SYT2_MOUSE

Protein

Synaptotagmin-2

Gene

Syt2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone.

    Cofactori

    Binds 3 calcium ions per subunit. The ions are bound to the C2 domains By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi172 – 1721Calcium 2; via carbonyl oxygenBy similarity
    Metal bindingi173 – 1731Calcium 1By similarity
    Metal bindingi173 – 1731Calcium 2By similarity
    Metal bindingi179 – 1791Calcium 1By similarity
    Metal bindingi231 – 2311Calcium 1By similarity
    Metal bindingi231 – 2311Calcium 2By similarity
    Metal bindingi232 – 2321Calcium 1; via carbonyl oxygenBy similarity
    Metal bindingi233 – 2331Calcium 1By similarity
    Metal bindingi233 – 2331Calcium 2By similarity
    Metal bindingi233 – 2331Calcium 3By similarity
    Metal bindingi236 – 2361Calcium 3By similarity
    Metal bindingi237 – 2371Calcium 3; via carbonyl oxygenBy similarity
    Metal bindingi239 – 2391Calcium 2By similarity
    Metal bindingi239 – 2391Calcium 3By similarity

    GO - Molecular functioni

    1. calcium-dependent phospholipid binding Source: MGI
    2. calcium ion binding Source: MGI
    3. protein binding Source: IntAct
    4. transporter activity Source: InterPro

    Keywords - Ligandi

    Calcium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Synaptotagmin-2
    Alternative name(s):
    Synaptotagmin II
    Short name:
    SytII
    Gene namesi
    Name:Syt2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:99666. Syt2.

    Subcellular locationi

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. chromaffin granule membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW
    4. plasma membrane Source: MGI
    5. synaptic vesicle membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cytoplasm, Cytoplasmic vesicle, Membrane, Synapse

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 422422Synaptotagmin-2PRO_0000183943Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi32 – 321N-linked (GlcNAc...)Sequence Analysis
    Modified residuei230 – 2301Phosphotyrosine1 Publication

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiP46097.
    PaxDbiP46097.
    PRIDEiP46097.

    PTM databases

    PhosphoSiteiP46097.

    Expressioni

    Gene expression databases

    ArrayExpressiP46097.
    BgeeiP46097.
    CleanExiMM_SYT2.
    GenevestigatoriP46097.

    Interactioni

    Subunit structurei

    Homotetramer Probable. Interacts with SCAMP5 By similarity. Interacts with stonin 2.By similarityCurated

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SCAMP5Q8TAC92EBI-457969,EBI-2695784From a different organism.
    Stx1aO355262EBI-457969,EBI-400878

    Protein-protein interaction databases

    BioGridi203612. 3 interactions.
    DIPiDIP-32645N.
    IntActiP46097. 9 interactions.
    MINTiMINT-2411200.

    Structurei

    Secondary structure

    1
    422
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi46 – 5712

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2NP0X-ray2.62B40-60[»]
    4KBBX-ray2.30C/D8-61[»]
    ProteinModelPortaliP46097.
    SMRiP46097. Positions 141-419.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP46097.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 6060VesicularSequence AnalysisAdd
    BLAST
    Topological domaini88 – 422335CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei61 – 8727HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini156 – 24590C2 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini287 – 37892C2 2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni136 – 382247Phospholipid bindingCuratedAdd
    BLAST

    Domaini

    The first C2 domain mediates Ca2+-dependent phospholipid binding.
    The second C2 domain mediates interaction with Stonin 2.

    Sequence similaritiesi

    Belongs to the synaptotagmin family.Curated
    Contains 2 C2 domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5038.
    GeneTreeiENSGT00620000087641.
    HOGENOMiHOG000232127.
    HOVERGENiHBG005010.
    InParanoidiP46097.
    OrthoDBiEOG78PV8W.
    PhylomeDBiP46097.
    TreeFamiTF315600.

    Family and domain databases

    Gene3Di2.60.40.150. 2 hits.
    InterProiIPR000008. C2_dom.
    IPR001565. Synaptotagmin.
    [Graphical view]
    PfamiPF00168. C2. 2 hits.
    [Graphical view]
    PRINTSiPR00360. C2DOMAIN.
    PR00399. SYNAPTOTAGMN.
    SMARTiSM00239. C2. 2 hits.
    [Graphical view]
    SUPFAMiSSF49562. SSF49562. 2 hits.
    PROSITEiPS50004. C2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P46097-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRNIFKRNQE PNVAPATTTA TMPLAPVAPA DNSTESTGPG ESQEDMFAKL    50
    KEKFFNEINK IPLPPWALIA MAVVAGLLLL TCCFCICKKC CCKKKKNKKE 100
    KGKGMKNAMN MKDMKGGQDD DDAETGLTEG EGEGEEEKEP ENLGKLQFSL 150
    DYDFQANQLT VGVLQAAELP ALDMGGTSDP YVKVFLLPDK KKKYETKVHR 200
    KTLNPAFNET FTFKVPYQEL AGKTLVMAIY DFDRFSKHDI IGEVKVPMNT 250
    VDLGQPIEEW RDLQGGEKEE PEKLGDICTS LRYVPTAGKL TVCILEAKNL 300
    KKMDVGGLSD PYVKIHLMQN GKRLKKKKTT VKKKTLNPYF NESFSFEIPF 350
    EQIQKVQVVV TVLDYDKLGK NEAIGKIFVG SNATGTELRH WSDMLANPRR 400
    PIAQWHSLKP EEEVDALLGK NK 422
    Length:422
    Mass (Da):47,263
    Last modified:November 1, 1995 - v1
    Checksum:iB4BD13FF70E0481B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti221 – 2211A → G(PubMed:16141072)Curated
    Sequence conflicti221 – 2211A → G(PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D37793 mRNA. Translation: BAA07041.1.
    AF257303 Genomic DNA. Translation: AAF68987.1.
    AF257304 mRNA. Translation: AAF68988.1.
    AK036357 mRNA. Translation: BAC29397.1.
    BC027019 mRNA. Translation: AAH27019.1.
    CCDSiCCDS15312.1.
    PIRiA55417.
    RefSeqiNP_033333.2. NM_009307.3.
    XP_006529385.1. XM_006529322.1.
    XP_006529386.1. XM_006529323.1.
    UniGeneiMm.5102.

    Genome annotation databases

    EnsembliENSMUST00000121990; ENSMUSP00000112438; ENSMUSG00000026452.
    GeneIDi20980.
    KEGGimmu:20980.
    UCSCiuc007csj.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D37793 mRNA. Translation: BAA07041.1 .
    AF257303 Genomic DNA. Translation: AAF68987.1 .
    AF257304 mRNA. Translation: AAF68988.1 .
    AK036357 mRNA. Translation: BAC29397.1 .
    BC027019 mRNA. Translation: AAH27019.1 .
    CCDSi CCDS15312.1.
    PIRi A55417.
    RefSeqi NP_033333.2. NM_009307.3.
    XP_006529385.1. XM_006529322.1.
    XP_006529386.1. XM_006529323.1.
    UniGenei Mm.5102.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2NP0 X-ray 2.62 B 40-60 [» ]
    4KBB X-ray 2.30 C/D 8-61 [» ]
    ProteinModelPortali P46097.
    SMRi P46097. Positions 141-419.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 203612. 3 interactions.
    DIPi DIP-32645N.
    IntActi P46097. 9 interactions.
    MINTi MINT-2411200.

    PTM databases

    PhosphoSitei P46097.

    Proteomic databases

    MaxQBi P46097.
    PaxDbi P46097.
    PRIDEi P46097.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000121990 ; ENSMUSP00000112438 ; ENSMUSG00000026452 .
    GeneIDi 20980.
    KEGGi mmu:20980.
    UCSCi uc007csj.1. mouse.

    Organism-specific databases

    CTDi 127833.
    MGIi MGI:99666. Syt2.

    Phylogenomic databases

    eggNOGi COG5038.
    GeneTreei ENSGT00620000087641.
    HOGENOMi HOG000232127.
    HOVERGENi HBG005010.
    InParanoidi P46097.
    OrthoDBi EOG78PV8W.
    PhylomeDBi P46097.
    TreeFami TF315600.

    Miscellaneous databases

    EvolutionaryTracei P46097.
    NextBioi 299968.
    PROi P46097.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P46097.
    Bgeei P46097.
    CleanExi MM_SYT2.
    Genevestigatori P46097.

    Family and domain databases

    Gene3Di 2.60.40.150. 2 hits.
    InterProi IPR000008. C2_dom.
    IPR001565. Synaptotagmin.
    [Graphical view ]
    Pfami PF00168. C2. 2 hits.
    [Graphical view ]
    PRINTSi PR00360. C2DOMAIN.
    PR00399. SYNAPTOTAGMN.
    SMARTi SM00239. C2. 2 hits.
    [Graphical view ]
    SUPFAMi SSF49562. SSF49562. 2 hits.
    PROSITEi PS50004. C2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Inositol-1,3,4,5-tetrakisphosphate binding to C2B domain of IP4BP/synaptotagmin II."
      Fukuda M., Aruga J., Niinobe M., Aimoto S., Mikoshiba K.
      J. Biol. Chem. 269:29206-29211(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Genomic structure of the murine Syt2 gene."
      Adachi R., Teich A.H., Nigam R.
      Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Cerebellum.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Eye.
    5. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
      Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
      J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-230, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.

    Entry informationi

    Entry nameiSYT2_MOUSE
    AccessioniPrimary (citable) accession number: P46097
    Secondary accession number(s): Q8R0E1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 123 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3