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P46097

- SYT2_MOUSE

UniProt

P46097 - SYT2_MOUSE

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Protein

Synaptotagmin-2

Gene
Syt2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone.

Cofactori

Binds 3 calcium ions per subunit. The ions are bound to the C2 domains By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi172 – 1721Calcium 2; via carbonyl oxygen By similarity
Metal bindingi173 – 1731Calcium 1 By similarity
Metal bindingi173 – 1731Calcium 2 By similarity
Metal bindingi179 – 1791Calcium 1 By similarity
Metal bindingi231 – 2311Calcium 1 By similarity
Metal bindingi231 – 2311Calcium 2 By similarity
Metal bindingi232 – 2321Calcium 1; via carbonyl oxygen By similarity
Metal bindingi233 – 2331Calcium 1 By similarity
Metal bindingi233 – 2331Calcium 2 By similarity
Metal bindingi233 – 2331Calcium 3 By similarity
Metal bindingi236 – 2361Calcium 3 By similarity
Metal bindingi237 – 2371Calcium 3; via carbonyl oxygen By similarity
Metal bindingi239 – 2391Calcium 2 By similarity
Metal bindingi239 – 2391Calcium 3 By similarity

GO - Molecular functioni

  1. calcium-dependent phospholipid binding Source: MGI
  2. calcium ion binding Source: MGI
  3. protein binding Source: IntAct
  4. transporter activity Source: InterPro
Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptotagmin-2
Alternative name(s):
Synaptotagmin II
Short name:
SytII
Gene namesi
Name:Syt2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:99666. Syt2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 6060Vesicular Reviewed predictionAdd
BLAST
Transmembranei61 – 8727Helical; Reviewed predictionAdd
BLAST
Topological domaini88 – 422335Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. chromaffin granule membrane Source: UniProtKB-SubCell
  3. integral component of membrane Source: UniProtKB-KW
  4. plasma membrane Source: MGI
  5. synaptic vesicle membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasm, Cytoplasmic vesicle, Membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 422422Synaptotagmin-2PRO_0000183943Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi32 – 321N-linked (GlcNAc...) Reviewed prediction
Modified residuei230 – 2301Phosphotyrosine1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP46097.
PaxDbiP46097.
PRIDEiP46097.

PTM databases

PhosphoSiteiP46097.

Expressioni

Gene expression databases

ArrayExpressiP46097.
BgeeiP46097.
CleanExiMM_SYT2.
GenevestigatoriP46097.

Interactioni

Subunit structurei

Homotetramer Inferred. Interacts with SCAMP5 By similarity. Interacts with stonin 2.

Binary interactionsi

WithEntry#Exp.IntActNotes
SCAMP5Q8TAC92EBI-457969,EBI-2695784From a different organism.
Stx1aO355262EBI-457969,EBI-400878

Protein-protein interaction databases

BioGridi203612. 3 interactions.
DIPiDIP-32645N.
IntActiP46097. 9 interactions.
MINTiMINT-2411200.

Structurei

Secondary structure

1
422
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi46 – 5712

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2NP0X-ray2.62B40-60[»]
4KBBX-ray2.30C/D8-61[»]
ProteinModelPortaliP46097.
SMRiP46097. Positions 141-419.

Miscellaneous databases

EvolutionaryTraceiP46097.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini156 – 24590C2 1Add
BLAST
Domaini287 – 37892C2 2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni136 – 382247Phospholipid binding InferredAdd
BLAST

Domaini

The first C2 domain mediates Ca2+-dependent phospholipid binding.
The second C2 domain mediates interaction with Stonin 2.

Sequence similaritiesi

Belongs to the synaptotagmin family.
Contains 2 C2 domains.

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5038.
GeneTreeiENSGT00620000087641.
HOGENOMiHOG000232127.
HOVERGENiHBG005010.
InParanoidiP46097.
OrthoDBiEOG78PV8W.
PhylomeDBiP46097.
TreeFamiTF315600.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR001565. Synaptotagmin.
[Graphical view]
PfamiPF00168. C2. 2 hits.
[Graphical view]
PRINTSiPR00360. C2DOMAIN.
PR00399. SYNAPTOTAGMN.
SMARTiSM00239. C2. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
PROSITEiPS50004. C2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P46097-1 [UniParc]FASTAAdd to Basket

« Hide

MRNIFKRNQE PNVAPATTTA TMPLAPVAPA DNSTESTGPG ESQEDMFAKL    50
KEKFFNEINK IPLPPWALIA MAVVAGLLLL TCCFCICKKC CCKKKKNKKE 100
KGKGMKNAMN MKDMKGGQDD DDAETGLTEG EGEGEEEKEP ENLGKLQFSL 150
DYDFQANQLT VGVLQAAELP ALDMGGTSDP YVKVFLLPDK KKKYETKVHR 200
KTLNPAFNET FTFKVPYQEL AGKTLVMAIY DFDRFSKHDI IGEVKVPMNT 250
VDLGQPIEEW RDLQGGEKEE PEKLGDICTS LRYVPTAGKL TVCILEAKNL 300
KKMDVGGLSD PYVKIHLMQN GKRLKKKKTT VKKKTLNPYF NESFSFEIPF 350
EQIQKVQVVV TVLDYDKLGK NEAIGKIFVG SNATGTELRH WSDMLANPRR 400
PIAQWHSLKP EEEVDALLGK NK 422
Length:422
Mass (Da):47,263
Last modified:November 1, 1995 - v1
Checksum:iB4BD13FF70E0481B
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti221 – 2211A → G1 Publication
Sequence conflicti221 – 2211A → G1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D37793 mRNA. Translation: BAA07041.1.
AF257303 Genomic DNA. Translation: AAF68987.1.
AF257304 mRNA. Translation: AAF68988.1.
AK036357 mRNA. Translation: BAC29397.1.
BC027019 mRNA. Translation: AAH27019.1.
CCDSiCCDS15312.1.
PIRiA55417.
RefSeqiNP_033333.2. NM_009307.3.
XP_006529385.1. XM_006529322.1.
XP_006529386.1. XM_006529323.1.
UniGeneiMm.5102.

Genome annotation databases

EnsembliENSMUST00000121990; ENSMUSP00000112438; ENSMUSG00000026452.
GeneIDi20980.
KEGGimmu:20980.
UCSCiuc007csj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D37793 mRNA. Translation: BAA07041.1 .
AF257303 Genomic DNA. Translation: AAF68987.1 .
AF257304 mRNA. Translation: AAF68988.1 .
AK036357 mRNA. Translation: BAC29397.1 .
BC027019 mRNA. Translation: AAH27019.1 .
CCDSi CCDS15312.1.
PIRi A55417.
RefSeqi NP_033333.2. NM_009307.3.
XP_006529385.1. XM_006529322.1.
XP_006529386.1. XM_006529323.1.
UniGenei Mm.5102.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2NP0 X-ray 2.62 B 40-60 [» ]
4KBB X-ray 2.30 C/D 8-61 [» ]
ProteinModelPortali P46097.
SMRi P46097. Positions 141-419.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 203612. 3 interactions.
DIPi DIP-32645N.
IntActi P46097. 9 interactions.
MINTi MINT-2411200.

PTM databases

PhosphoSitei P46097.

Proteomic databases

MaxQBi P46097.
PaxDbi P46097.
PRIDEi P46097.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000121990 ; ENSMUSP00000112438 ; ENSMUSG00000026452 .
GeneIDi 20980.
KEGGi mmu:20980.
UCSCi uc007csj.1. mouse.

Organism-specific databases

CTDi 127833.
MGIi MGI:99666. Syt2.

Phylogenomic databases

eggNOGi COG5038.
GeneTreei ENSGT00620000087641.
HOGENOMi HOG000232127.
HOVERGENi HBG005010.
InParanoidi P46097.
OrthoDBi EOG78PV8W.
PhylomeDBi P46097.
TreeFami TF315600.

Miscellaneous databases

EvolutionaryTracei P46097.
NextBioi 299968.
PROi P46097.
SOURCEi Search...

Gene expression databases

ArrayExpressi P46097.
Bgeei P46097.
CleanExi MM_SYT2.
Genevestigatori P46097.

Family and domain databases

Gene3Di 2.60.40.150. 2 hits.
InterProi IPR000008. C2_dom.
IPR001565. Synaptotagmin.
[Graphical view ]
Pfami PF00168. C2. 2 hits.
[Graphical view ]
PRINTSi PR00360. C2DOMAIN.
PR00399. SYNAPTOTAGMN.
SMARTi SM00239. C2. 2 hits.
[Graphical view ]
SUPFAMi SSF49562. SSF49562. 2 hits.
PROSITEi PS50004. C2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Inositol-1,3,4,5-tetrakisphosphate binding to C2B domain of IP4BP/synaptotagmin II."
    Fukuda M., Aruga J., Niinobe M., Aimoto S., Mikoshiba K.
    J. Biol. Chem. 269:29206-29211(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Genomic structure of the murine Syt2 gene."
    Adachi R., Teich A.H., Nigam R.
    Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Cerebellum.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Eye.
  5. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
    Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
    J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-230, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiSYT2_MOUSE
AccessioniPrimary (citable) accession number: P46097
Secondary accession number(s): Q8R0E1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 9, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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