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Protein

Synaptotagmin-1

Gene

Syt1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse (PubMed:7961887). It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca2+-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca2+-independent manner; these are neurexins, syntaxin and AP2. Plays a role in dendrite formation by melanocytes (By similarity).By similarity1 Publication

Cofactori

Ca2+By similarityNote: Binds 3 Ca2+ ions per subunit. The ions are bound to the C2 domains.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi171Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi172Calcium 1By similarity1
Metal bindingi172Calcium 2By similarity1
Metal bindingi178Calcium 1By similarity1
Metal bindingi230Calcium 1By similarity1
Metal bindingi230Calcium 2By similarity1
Metal bindingi231Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi232Calcium 1By similarity1
Metal bindingi232Calcium 2By similarity1
Metal bindingi232Calcium 3By similarity1
Metal bindingi235Calcium 3By similarity1
Metal bindingi236Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi238Calcium 2By similarity1
Metal bindingi238Calcium 3By similarity1

GO - Molecular functioni

GO - Biological processi

  • brain development Source: Ensembl
  • cellular response to calcium ion Source: ParkinsonsUK-UCL
  • detection of calcium ion Source: Ensembl
  • fast, calcium ion-dependent exocytosis of neurotransmitter Source: ParkinsonsUK-UCL
  • neurotransmitter secretion Source: MGI
  • positive regulation of calcium ion-dependent exocytosis Source: Ensembl
  • positive regulation of dendrite extension Source: MGI
  • positive regulation of synaptic transmission Source: ParkinsonsUK-UCL
  • positive regulation of vesicle fusion Source: Ensembl
  • protein heterooligomerization Source: Ensembl
  • protein homooligomerization Source: Ensembl
  • regulation of calcium ion-dependent exocytosis Source: CACAO
  • regulation of dopamine secretion Source: ParkinsonsUK-UCL
  • regulation of regulated secretory pathway Source: ParkinsonsUK-UCL
  • regulation of synaptic transmission, glutamatergic Source: ParkinsonsUK-UCL
  • synaptic vesicle endocytosis Source: GO_Central
  • synaptic vesicle exocytosis Source: MGI
  • vesicle docking Source: Ensembl
  • vesicle fusion Source: GO_Central

Keywordsi

Biological processDifferentiation
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-181430. Norepinephrine Neurotransmitter Release Cycle.
R-MMU-210500. Glutamate Neurotransmitter Release Cycle.
R-MMU-264642. Acetylcholine Neurotransmitter Release Cycle.
R-MMU-8856825. Cargo recognition for clathrin-mediated endocytosis.
R-MMU-8856828. Clathrin-mediated endocytosis.
R-MMU-888590. GABA synthesis, release, reuptake and degradation.

Protein family/group databases

TCDBi9.A.48.1.1. the unconventional protein secretion (ups) system.

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptotagmin-1
Alternative name(s):
Synaptotagmin I
Short name:
SytI
p65
Gene namesi
Name:Syt1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:99667. Syt1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 57VesicularSequence analysisAdd BLAST57
Transmembranei58 – 79HelicalSequence analysisAdd BLAST22
Topological domaini80 – 421CytoplasmicSequence analysisAdd BLAST342

Keywords - Cellular componenti

Cell junction, Cytoplasm, Cytoplasmic vesicle, Membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001839381 – 421Synaptotagmin-1Add BLAST421

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi24N-linked (GlcNAc...) asparagineBy similarity1
Lipidationi74S-palmitoyl cysteineBy similarity1
Lipidationi75S-palmitoyl cysteineBy similarity1
Lipidationi77S-palmitoyl cysteineBy similarity1
Lipidationi79S-palmitoyl cysteineBy similarity1
Lipidationi82S-palmitoyl cysteineBy similarity1
Modified residuei128PhosphothreonineBy similarity1
Modified residuei229PhosphotyrosineCombined sources1
Modified residuei264PhosphoserineCombined sources1
Modified residuei342PhosphoserineBy similarity1
Modified residuei344PhosphoserineBy similarity1

Keywords - PTMi

Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

MaxQBiP46096.
PaxDbiP46096.
PRIDEiP46096.

PTM databases

iPTMnetiP46096.
PhosphoSitePlusiP46096.
SwissPalmiP46096.

Expressioni

Gene expression databases

BgeeiENSMUSG00000035864.
CleanExiMM_SYT1.
ExpressionAtlasiP46096. baseline and differential.
GenevisibleiP46096. MM.

Interactioni

Subunit structurei

Homotetramer (Probable). Interacts with SCAMP5, STON2, SV2A, SV2B, SV2C and RIMS1 (By similarity). Forms a complex with SV2B, syntaxin 1 and SNAP25.By similarityCurated1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203611. 5 interactors.
DIPiDIP-31322N.
IntActiP46096. 19 interactors.
MINTiMINT-4136555.
STRINGi10090.ENSMUSP00000063293.

Structurei

Secondary structure

1421
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi144 – 152Combined sources9
Turni153 – 156Combined sources4
Beta strandi157 – 167Combined sources11
Beta strandi179 – 186Combined sources8
Beta strandi205 – 213Combined sources9
Helixi216 – 219Combined sources4
Beta strandi223 – 230Combined sources8
Beta strandi233 – 235Combined sources3
Beta strandi239 – 246Combined sources8
Helixi247 – 249Combined sources3
Beta strandi256 – 261Combined sources6
Beta strandi275 – 283Combined sources9
Turni284 – 287Combined sources4
Beta strandi288 – 298Combined sources11
Beta strandi310 – 318Combined sources9
Beta strandi321 – 327Combined sources7
Beta strandi338 – 346Combined sources9
Helixi349 – 351Combined sources3
Beta strandi356 – 363Combined sources8
Beta strandi371 – 379Combined sources9
Helixi384 – 395Combined sources12
Beta strandi401 – 406Combined sources6
Helixi410 – 416Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5T0RX-ray1.95A140-265[»]
5T0SX-ray1.42A271-421[»]
ProteinModelPortaliP46096.
SMRiP46096.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini143 – 244C2 1PROSITE-ProRule annotationAdd BLAST102
Domaini274 – 377C2 2PROSITE-ProRule annotationAdd BLAST104

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni135 – 381Phospholipid bindingCuratedAdd BLAST247

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi80 – 119Lys-richAdd BLAST40

Domaini

The first C2 domain mediates Ca2+-dependent phospholipid binding.By similarity
The second C2 domain mediates interaction with SV2A and probably with STN2.By similarity

Sequence similaritiesi

Belongs to the synaptotagmin family.Curated

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IT3Q. Eukaryota.
ENOG410Z15P. LUCA.
GeneTreeiENSGT00760000118973.
HOGENOMiHOG000232127.
HOVERGENiHBG005010.
InParanoidiP46096.
KOiK15290.
OMAiHNATEPA.
OrthoDBiEOG091G0XMQ.
PhylomeDBiP46096.
TreeFamiTF315600.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
InterProiView protein in InterPro
IPR000008. C2_dom.
IPR035892. C2_domain_sf.
IPR001565. Synaptotagmin.
IPR015428. Synaptotagmin1/2.
PANTHERiPTHR10024:SF239. PTHR10024:SF239. 1 hit.
PfamiView protein in Pfam
PF00168. C2. 2 hits.
PRINTSiPR00360. C2DOMAIN.
PR00399. SYNAPTOTAGMN.
SMARTiView protein in SMART
SM00239. C2. 2 hits.
SUPFAMiSSF49562. SSF49562. 2 hits.
PROSITEiView protein in PROSITE
PS50004. C2. 2 hits.

Sequencei

Sequence statusi: Complete.

P46096-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSASRPEAL AAPVTTVATL VPHNATEPAS PGEGKEDAFS KLKQKFMNEL
60 70 80 90 100
HKIPLPPWAL IAIAIVAVLL VVTCCFCVCK KCLFKKKNKK KGKEKGGKNA
110 120 130 140 150
INMKDVKDLG KTMKDQALKD DDAETGLTDG EEKEEPKEEE KLGKLQYSLD
160 170 180 190 200
YDFQNNQLLV GIIQAAELPA LDMGGTSDPY VKVFLLPDKK KKFETKVHRK
210 220 230 240 250
TLNPVFNEQF TFKVPYSELG GKTLVMAVYD FDRFSKHDII GEFKVPMNTV
260 270 280 290 300
DFGHVTEEWR DLQSAEKEEQ EKLGDICFSL RYVPTAGKLT VVILEAKNLK
310 320 330 340 350
KMDVGGLSDP YVKIHLMQNG KRLKKKKTTI KKNTLNPYYN ESFSFEVPFE
360 370 380 390 400
QIQKVQVVVT VLDYDKIGKN DAIGKVFVGY NSTGAELRHW SDMLANPRRP
410 420
IAQWHTLQVE EEVDAMLAVK K
Length:421
Mass (Da):47,418
Last modified:November 1, 1995 - v1
Checksum:i7FDEFF37170BD169
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D37792 mRNA. Translation: BAA07040.1.
AK078790 mRNA. Translation: BAC37395.1.
BC042519 mRNA. Translation: AAH42519.1.
CCDSiCCDS24163.1.
RefSeqiNP_001239270.1. NM_001252341.1.
NP_001239271.1. NM_001252342.1.
NP_033332.1. NM_009306.3.
XP_006513509.1. XM_006513446.1.
XP_006513510.1. XM_006513447.1.
XP_006513511.1. XM_006513448.1.
XP_017169355.1. XM_017313866.1.
UniGeneiMm.289702.
Mm.489829.

Genome annotation databases

EnsembliENSMUST00000064054; ENSMUSP00000063293; ENSMUSG00000035864.
ENSMUST00000105276; ENSMUSP00000100912; ENSMUSG00000035864.
GeneIDi20979.
KEGGimmu:20979.
UCSCiuc007gzg.2. mouse.

Similar proteinsi

Entry informationi

Entry nameiSYT1_MOUSE
AccessioniPrimary (citable) accession number: P46096
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 25, 2017
This is version 171 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families