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P46096

- SYT1_MOUSE

UniProt

P46096 - SYT1_MOUSE

Protein

Synaptotagmin-1

Gene

Syt1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 139 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca2+-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca2+-independent manner; these are neurexins, syntaxin and AP2.

    Cofactori

    Binds 3 calcium ions per subunit. The ions are bound to the C2 domains By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi171 – 1711Calcium 2; via carbonyl oxygenBy similarity
    Metal bindingi172 – 1721Calcium 1By similarity
    Metal bindingi172 – 1721Calcium 2By similarity
    Metal bindingi178 – 1781Calcium 1By similarity
    Metal bindingi230 – 2301Calcium 1By similarity
    Metal bindingi230 – 2301Calcium 2By similarity
    Metal bindingi231 – 2311Calcium 1; via carbonyl oxygenBy similarity
    Metal bindingi232 – 2321Calcium 1By similarity
    Metal bindingi232 – 2321Calcium 2By similarity
    Metal bindingi232 – 2321Calcium 3By similarity
    Metal bindingi235 – 2351Calcium 3By similarity
    Metal bindingi236 – 2361Calcium 3; via carbonyl oxygenBy similarity
    Metal bindingi238 – 2381Calcium 2By similarity
    Metal bindingi238 – 2381Calcium 3By similarity

    GO - Molecular functioni

    1. calcium-dependent phospholipid binding Source: MGI
    2. calcium-dependent protein binding Source: HGNC
    3. calcium ion binding Source: MGI
    4. low-density lipoprotein particle receptor binding Source: MGI
    5. phosphatidylinositol-4,5-bisphosphate binding Source: Ensembl
    6. phosphatidylserine binding Source: Ensembl
    7. protein binding Source: UniProtKB
    8. transporter activity Source: InterPro

    GO - Biological processi

    1. neurotransmitter secretion Source: MGI
    2. positive regulation of calcium ion-dependent exocytosis Source: Ensembl
    3. positive regulation of synaptic transmission Source: ParkinsonsUK-UCL
    4. positive regulation of vesicle fusion Source: Ensembl
    5. response to calcium ion Source: Ensembl
    6. synaptic vesicle exocytosis Source: MGI
    7. vesicle docking Source: Ensembl

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_205038. Acetylcholine Neurotransmitter Release Cycle.

    Protein family/group databases

    TCDBi9.A.48.1.1. the unconventional protein secretion (ups) system.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Synaptotagmin-1
    Alternative name(s):
    Synaptotagmin I
    Short name:
    SytI
    p65
    Gene namesi
    Name:Syt1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 10

    Organism-specific databases

    MGIiMGI:99667. Syt1.

    Subcellular locationi

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. chromaffin granule membrane Source: UniProtKB-SubCell
    3. dense core granule Source: Ensembl
    4. excitatory synapse Source: Ensembl
    5. integral component of membrane Source: UniProtKB-KW
    6. neuron projection Source: ParkinsonsUK-UCL
    7. plasma membrane Source: UniProtKB
    8. presynaptic membrane Source: MGI
    9. secretory granule Source: MGI
    10. synaptic vesicle Source: UniProtKB
    11. synaptic vesicle membrane Source: MGI

    Keywords - Cellular componenti

    Cell junction, Cytoplasm, Cytoplasmic vesicle, Membrane, Synapse

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 421421Synaptotagmin-1PRO_0000183938Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi24 – 241N-linked (GlcNAc...)By similarity
    Lipidationi74 – 741S-palmitoyl cysteineBy similarity
    Lipidationi75 – 751S-palmitoyl cysteineBy similarity
    Lipidationi77 – 771S-palmitoyl cysteineBy similarity
    Lipidationi79 – 791S-palmitoyl cysteineBy similarity
    Lipidationi82 – 821S-palmitoyl cysteineBy similarity
    Modified residuei128 – 1281PhosphothreonineBy similarity
    Modified residuei229 – 2291Phosphotyrosine1 Publication

    Keywords - PTMi

    Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

    Proteomic databases

    MaxQBiP46096.
    PaxDbiP46096.
    PRIDEiP46096.

    PTM databases

    PhosphoSiteiP46096.

    Expressioni

    Gene expression databases

    ArrayExpressiP46096.
    BgeeiP46096.
    CleanExiMM_SYT1.
    GenevestigatoriP46096.

    Interactioni

    Subunit structurei

    Homotetramer Probable. Interacts with SCAMP5, STON2, SV2A, SV2B, SV2C and RIMS1 By similarity. Forms a complex with SV2B, syntaxin 1 and SNAP25.By similarity1 PublicationCurated

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SCAMP5Q8TAC92EBI-445340,EBI-2695784From a different organism.

    Protein-protein interaction databases

    BioGridi203611. 4 interactions.
    DIPiDIP-31322N.
    IntActiP46096. 13 interactions.
    MINTiMINT-4136555.
    STRINGi10090.ENSMUSP00000100912.

    Structurei

    3D structure databases

    ProteinModelPortaliP46096.
    SMRiP46096. Positions 140-418.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 5757VesicularSequence AnalysisAdd
    BLAST
    Topological domaini80 – 421342CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei58 – 7922HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini143 – 244102C2 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini274 – 377104C2 2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni135 – 381247Phospholipid bindingCuratedAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi80 – 11940Lys-richAdd
    BLAST

    Domaini

    The first C2 domain mediates Ca2+-dependent phospholipid binding.
    The second C2 domain mediates interaction with SV2A and STN2.By similarity

    Sequence similaritiesi

    Belongs to the synaptotagmin family.Curated
    Contains 2 C2 domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5038.
    GeneTreeiENSGT00620000087641.
    HOGENOMiHOG000232127.
    HOVERGENiHBG005010.
    InParanoidiP46096.
    KOiK15290.
    OMAiHDIIGEY.
    OrthoDBiEOG78PV8W.
    PhylomeDBiP46096.
    TreeFamiTF315600.

    Family and domain databases

    Gene3Di2.60.40.150. 2 hits.
    InterProiIPR000008. C2_dom.
    IPR001565. Synaptotagmin.
    IPR015428. Synaptotagmin1.
    [Graphical view]
    PANTHERiPTHR10024:SF183. PTHR10024:SF183. 1 hit.
    PfamiPF00168. C2. 2 hits.
    [Graphical view]
    PRINTSiPR00360. C2DOMAIN.
    PR00399. SYNAPTOTAGMN.
    SMARTiSM00239. C2. 2 hits.
    [Graphical view]
    SUPFAMiSSF49562. SSF49562. 2 hits.
    PROSITEiPS50004. C2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P46096-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVSASRPEAL AAPVTTVATL VPHNATEPAS PGEGKEDAFS KLKQKFMNEL    50
    HKIPLPPWAL IAIAIVAVLL VVTCCFCVCK KCLFKKKNKK KGKEKGGKNA 100
    INMKDVKDLG KTMKDQALKD DDAETGLTDG EEKEEPKEEE KLGKLQYSLD 150
    YDFQNNQLLV GIIQAAELPA LDMGGTSDPY VKVFLLPDKK KKFETKVHRK 200
    TLNPVFNEQF TFKVPYSELG GKTLVMAVYD FDRFSKHDII GEFKVPMNTV 250
    DFGHVTEEWR DLQSAEKEEQ EKLGDICFSL RYVPTAGKLT VVILEAKNLK 300
    KMDVGGLSDP YVKIHLMQNG KRLKKKKTTI KKNTLNPYYN ESFSFEVPFE 350
    QIQKVQVVVT VLDYDKIGKN DAIGKVFVGY NSTGAELRHW SDMLANPRRP 400
    IAQWHTLQVE EEVDAMLAVK K 421
    Length:421
    Mass (Da):47,418
    Last modified:November 1, 1995 - v1
    Checksum:i7FDEFF37170BD169
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D37792 mRNA. Translation: BAA07040.1.
    AK078790 mRNA. Translation: BAC37395.1.
    BC042519 mRNA. Translation: AAH42519.1.
    CCDSiCCDS24163.1.
    RefSeqiNP_001239270.1. NM_001252341.1.
    NP_001239271.1. NM_001252342.1.
    NP_033332.1. NM_009306.3.
    XP_006513509.1. XM_006513446.1.
    XP_006513510.1. XM_006513447.1.
    XP_006513511.1. XM_006513448.1.
    UniGeneiMm.289702.
    Mm.489829.

    Genome annotation databases

    EnsembliENSMUST00000064054; ENSMUSP00000063293; ENSMUSG00000035864.
    ENSMUST00000105276; ENSMUSP00000100912; ENSMUSG00000035864.
    GeneIDi20979.
    KEGGimmu:20979.
    UCSCiuc007gzg.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D37792 mRNA. Translation: BAA07040.1 .
    AK078790 mRNA. Translation: BAC37395.1 .
    BC042519 mRNA. Translation: AAH42519.1 .
    CCDSi CCDS24163.1.
    RefSeqi NP_001239270.1. NM_001252341.1.
    NP_001239271.1. NM_001252342.1.
    NP_033332.1. NM_009306.3.
    XP_006513509.1. XM_006513446.1.
    XP_006513510.1. XM_006513447.1.
    XP_006513511.1. XM_006513448.1.
    UniGenei Mm.289702.
    Mm.489829.

    3D structure databases

    ProteinModelPortali P46096.
    SMRi P46096. Positions 140-418.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 203611. 4 interactions.
    DIPi DIP-31322N.
    IntActi P46096. 13 interactions.
    MINTi MINT-4136555.
    STRINGi 10090.ENSMUSP00000100912.

    Protein family/group databases

    TCDBi 9.A.48.1.1. the unconventional protein secretion (ups) system.

    PTM databases

    PhosphoSitei P46096.

    Proteomic databases

    MaxQBi P46096.
    PaxDbi P46096.
    PRIDEi P46096.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000064054 ; ENSMUSP00000063293 ; ENSMUSG00000035864 .
    ENSMUST00000105276 ; ENSMUSP00000100912 ; ENSMUSG00000035864 .
    GeneIDi 20979.
    KEGGi mmu:20979.
    UCSCi uc007gzg.2. mouse.

    Organism-specific databases

    CTDi 6857.
    MGIi MGI:99667. Syt1.

    Phylogenomic databases

    eggNOGi COG5038.
    GeneTreei ENSGT00620000087641.
    HOGENOMi HOG000232127.
    HOVERGENi HBG005010.
    InParanoidi P46096.
    KOi K15290.
    OMAi HDIIGEY.
    OrthoDBi EOG78PV8W.
    PhylomeDBi P46096.
    TreeFami TF315600.

    Enzyme and pathway databases

    Reactomei REACT_205038. Acetylcholine Neurotransmitter Release Cycle.

    Miscellaneous databases

    ChiTaRSi SYT1. mouse.
    NextBioi 299964.
    PROi P46096.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P46096.
    Bgeei P46096.
    CleanExi MM_SYT1.
    Genevestigatori P46096.

    Family and domain databases

    Gene3Di 2.60.40.150. 2 hits.
    InterProi IPR000008. C2_dom.
    IPR001565. Synaptotagmin.
    IPR015428. Synaptotagmin1.
    [Graphical view ]
    PANTHERi PTHR10024:SF183. PTHR10024:SF183. 1 hit.
    Pfami PF00168. C2. 2 hits.
    [Graphical view ]
    PRINTSi PR00360. C2DOMAIN.
    PR00399. SYNAPTOTAGMN.
    SMARTi SM00239. C2. 2 hits.
    [Graphical view ]
    SUPFAMi SSF49562. SSF49562. 2 hits.
    PROSITEi PS50004. C2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Inositol-1,3,4,5-tetrakisphosphate binding to C2B domain of IP4BP/synaptotagmin II."
      Fukuda M., Aruga J., Niinobe M., Aimoto S., Mikoshiba K.
      J. Biol. Chem. 269:29206-29211(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Testis.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Eye.
    4. Lubec G., Kang S.U., Sunyer B., Chen W.-Q.
      Submitted (JAN-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 183-189; 201-233; 245-260; 273-281; 289-297; 302-313; 333-366 AND 376-388, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: C57BL/6 and OF1.
      Tissue: Brain and Hippocampus.
    5. Cited for: INTERACTION WITH SV2B; SYNTAXIN-1 AND SNAP25.
    6. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
      Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
      J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-229, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.

    Entry informationi

    Entry nameiSYT1_MOUSE
    AccessioniPrimary (citable) accession number: P46096
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 139 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3