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P46093 (GPR4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
G-protein coupled receptor 4
Alternative name(s):
G-protein coupled receptor 19
Gene names
Name:GPR4
Synonyms:GPR19
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length362 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Proton-sensing receptor coupled to several G-proteins, including G(s), G13 and G(q)/G11 proteins, leading to cAMP production. Ref.9 Ref.11 Ref.12

Subcellular location

Cell membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Sequence caution

The sequence AAA63180.1 differs from that shown. Reason: Frameshift at position 347.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 362362G-protein coupled receptor 4
PRO_0000069512

Regions

Topological domain1 – 1717Extracellular Potential
Transmembrane18 – 4225Helical; Name=1; Potential
Topological domain43 – 5412Cytoplasmic Potential
Transmembrane55 – 7622Helical; Name=2; Potential
Topological domain77 – 9115Extracellular Potential
Transmembrane92 – 11322Helical; Name=3; Potential
Topological domain114 – 13219Cytoplasmic Potential
Transmembrane133 – 15422Helical; Name=4; Potential
Topological domain155 – 17925Extracellular Potential
Transmembrane180 – 20122Helical; Name=5; Potential
Topological domain202 – 22423Cytoplasmic Potential
Transmembrane225 – 24521Helical; Name=6; Potential
Topological domain246 – 26318Extracellular Potential
Transmembrane264 – 28421Helical; Name=7; Potential
Topological domain285 – 36278Cytoplasmic Potential

Amino acid modifications

Glycosylation31N-linked (GlcNAc...) Potential
Glycosylation1641N-linked (GlcNAc...) Potential
Disulfide bond90 ↔ 168 By similarity

Natural variations

Natural variant2951S → N.
Corresponds to variant rs36012326 [ dbSNP | Ensembl ].
VAR_049390

Experimental info

Sequence conflict571M → L in AAA79061. Ref.3
Sequence conflict691T → S in AAA79061. Ref.3
Sequence conflict1301R → C in AAH67535. Ref.8
Sequence conflict2551P → L in BAF83387. Ref.5
Sequence conflict3371A → V in AAA79061. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P46093 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: 6C75D97C36204541

FASTA36240,982
        10         20         30         40         50         60 
MGNHTWEGCH VDSRVDHLFP PSLYIFVIGV GLPTNCLALW AAYRQVQQRN ELGVYLMNLS 

        70         80         90        100        110        120 
IADLLYICTL PLWVDYFLHH DNWIHGPGSC KLFGFIFYTN IYISIAFLCC ISVDRYLAVA 

       130        140        150        160        170        180 
HPLRFARLRR VKTAVAVSSV VWATELGANS APLFHDELFR DRYNHTFCFE KFPMEGWVAW 

       190        200        210        220        230        240 
MNLYRVFVGF LFPWALMLLS YRGILRAVRG SVSTERQEKA KIKRLALSLI AIVLVCFAPY 

       250        260        270        280        290        300 
HVLLLSRSAI YLGRPWDCGF EERVFSAYHS SLAFTSLNCV ADPILYCLVN EGARSDVAKA 

       310        320        330        340        350        360 
LHNLLRFLAS DKPQEMANAS LTLETPLTSK RNSTAKAMTG SWAATPPSQG DQVQLKMLPP 


AQ 

« Hide

References

« Hide 'large scale' references
[1]"Isolation of three novel human genes encoding G protein-coupled receptors."
Heiber M., Docherty J.M., Shah G., Nguyen T., Cheng R., Heng H.H.Q., Marchese A., Tsui L.-C., Shi X., George S.R., O'Dowd B.F.
DNA Cell Biol. 14:25-35(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Isolation of a novel G protein-coupled receptor (GPR4) localized to chromosome 19q13.3."
Mahadevan M.S., Baird S., Bailly J.E., Shutler G.G., Sabourin L.A., Tsilfidis C., Neville C.E., Narang M., Korneluk R.G.
Genomics 30:84-88(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Cloning, sequencing and tissue distribution of two related G protein-coupled receptor candidates expressed prominently in human lung tissue."
An S., Tsai C., Goetzl E.J.
FEBS Lett. 375:121-124(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]Kaighin V.A., Martin A.L., Aronstam R.S.
Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[5]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lung.
[6]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[9]"Sphingosylphosphorylcholine and lysophosphatidylcholine are ligands for the G protein-coupled receptor GPR4."
Zhu K., Baudhuin L.M., Hong G., Williams F.S., Cristina K.L., Kabarowski J.H., Witte O.N., Xu Y.
J. Biol. Chem. 276:41325-41335(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: PRELIMINARY FUNCTION.
[10]Erratum
Zhu K., Baudhuin L.M., Hong G., Williams F.S., Cristina K.L., Kabarowski J.H., Witte O.N., Xu Y.
J. Biol. Chem. 280:43280-43280(2005)
Cited for: RETRACTION.
[11]"Proton-sensing G-protein-coupled receptors."
Ludwig M.-G., Vanek M., Guerini D., Gasser J.A., Jones C.E., Junker U., Hofstetter H., Wolf R.M., Seuwen K.
Nature 425:93-98(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[12]"Previously postulated 'ligand-independent' signaling of GPR4 is mediated through proton-sensing mechanisms."
Tobo M., Tomura H., Mogi C., Wang J.Q., Liu J.P., Komachi M., Damirin A., Kimura T., Murata N., Kurose H., Sato K., Okajima F.
Cell. Signal. 19:1745-1753(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L36148 Genomic DNA. Translation: AAA63180.1. Frameshift.
U21051 Genomic DNA. Translation: AAA98457.1.
U35399 mRNA. Translation: AAA79061.1.
EU432116 mRNA. Translation: ABY87915.1.
AK290698 mRNA. Translation: BAF83387.1.
AC011480 Genomic DNA. No translation available.
CH471126 Genomic DNA. Translation: EAW57368.1.
BC067535 mRNA. Translation: AAH67535.1.
BC067536 mRNA. Translation: AAH67536.1.
CCDSCCDS12669.1.
PIRA57641.
I53033.
S68207.
RefSeqNP_005273.1. NM_005282.2.
UniGeneHs.17170.

3D structure databases

ProteinModelPortalP46093.
SMRP46093. Positions 18-309.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000319744.

Protein family/group databases

GPCRDBSearch...

Polymorphism databases

DMDM1708027.

Proteomic databases

PaxDbP46093.
PRIDEP46093.

Protocols and materials databases

DNASU2828.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000323040; ENSP00000319744; ENSG00000177464.
GeneID2828.
KEGGhsa:2828.
UCSCuc002pcm.3. human.

Organism-specific databases

CTD2828.
GeneCardsGC19M046093.
HGNCHGNC:4497. GPR4.
HPAHPA014278.
MIM600551. gene.
neXtProtNX_P46093.
PharmGKBPA28886.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG149070.
HOGENOMHOG000004801.
HOVERGENHBG096696.
InParanoidP46093.
KOK04312.
OMANHTFCFE.
OrthoDBEOG7992QK.
PhylomeDBP46093.
TreeFamTF331803.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.

Gene expression databases

BgeeP46093.
CleanExHS_GPR19.
HS_GPR4.
GenevestigatorP46093.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR002276. GPR4_orph.
[Graphical view]
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00237. GPCRRHODOPSN.
PR01147. GPR4RECEPTOR.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiGPR4.
GenomeRNAi2828.
NextBio11159.
PROP46093.
SOURCESearch...

Entry information

Entry nameGPR4_HUMAN
AccessionPrimary (citable) accession number: P46093
Secondary accession number(s): A8K3T3, B0M0K1, Q6NWM4
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 1, 1996
Last modified: July 9, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries