Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P46089 (GPR3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
G-protein coupled receptor 3
Alternative name(s):
ACCA orphan receptor
Gene names
Name:GPR3
Synonyms:ACCA
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length330 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Orphan receptor with constitutive G(s) signaling activity that activate cyclic AMP. Has a potential role in modulating a number of brain functions, including behavioral responses to stress By similarity, amyloid-beta peptide generation in neurons and neurite outgrowth By similarity. Maintains also meiotic arrest in oocytes By similarity. Ref.9 Ref.11

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Expressed predominantly in the central nervous system, and at low levels in the lung, kidney, testis, ovary and eye. Highly expressed in regions of the brain implicated in the Alzheimer disease.

Induction

Overexpression stimulates amyloid-beta production.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Caution

Was originally (Ref.9) thought to be a receptor for sphingosine 1-phosphate. Ref.10 demonstrated that it is not the case.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

APPP05067-42EBI-3909653,EBI-302641

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 330330G-protein coupled receptor 3
PRO_0000069510

Regions

Topological domain1 – 4242Extracellular Potential
Transmembrane43 – 6220Helical; Name=1; Potential
Topological domain63 – 7412Cytoplasmic Potential
Transmembrane75 – 9824Helical; Name=2; Potential
Topological domain99 – 11012Extracellular Potential
Transmembrane111 – 13222Helical; Name=3; Potential
Topological domain133 – 15321Cytoplasmic Potential
Transmembrane154 – 17320Helical; Name=4; Potential
Topological domain174 – 19825Extracellular Potential
Transmembrane199 – 21719Helical; Name=5; Potential
Topological domain218 – 24528Cytoplasmic Potential
Transmembrane246 – 27227Helical; Name=6; Potential
Topological domain273 – 2775Extracellular Potential
Transmembrane278 – 29922Helical; Name=7; Potential
Topological domain300 – 33031Cytoplasmic Potential

Amino acid modifications

Modified residue3241Phosphoserine Potential
Modified residue3261Phosphoserine Potential
Modified residue3281Phosphoserine Potential
Lipidation3131S-palmitoyl cysteine By similarity
Glycosylation201N-linked (GlcNAc...) Potential

Natural variations

Natural variant2221R → H.
Corresponds to variant rs734852 [ dbSNP | Ensembl ].
VAR_011859

Sequences

Sequence LengthMass (Da)Tools
P46089 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 0F82E89200968D1E

FASTA33035,010
        10         20         30         40         50         60 
MMWGAGSPLA WLSAGSGNVN VSSVGPAEGP TGPAAPLPSP KAWDVVLCIS GTLVSCENAL 

        70         80         90        100        110        120 
VVAIIVGTPA FRAPMFLLVG SLAVADLLAG LGLVLHFAAV FCIGSAEMSL VLVGVLAMAF 

       130        140        150        160        170        180 
TASIGSLLAI TVDRYLSLYN ALTYYSETTV TRTYVMLALV WGGALGLGLL PVLAWNCLDG 

       190        200        210        220        230        240 
LTTCGVVYPL SKNHLVVLAI AFFMVFGIML QLYAQICRIV CRHAQQIALQ RHLLPASHYV 

       250        260        270        280        290        300 
ATRKGIATLA VVLGAFAACW LPFTVYCLLG DAHSPPLYTY LTLLPATYNS MINPIIYAFR 

       310        320        330 
NQDVQKVLWA VCCCCSSSKI PFRSRSPSDV 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and chromosomal localization of human genes encoding three closely related G protein-coupled receptors."
Song Z.-H., Modi W., Bonner T.I.
Genomics 28:347-349(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Isolation and chromosomal localization of a novel human G-protein-coupled receptor (GPR3) expressed predominantly in the central nervous system."
Iismaa T.P., Kiefer J., Liu M.L., Baker E., Sutherland G.R., Shine J.
Genomics 24:391-394(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Molecular cloning of an orphan G-protein-coupled receptor that constitutively activates adenylate cyclase."
Eggerickx D., Denef J.F., Labbe O., Hayashi Y., Refetoff S., Vassart G., Parmentier M., Libert F.
Biochem. J. 309:837-843(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[8]"Cloning of human genes encoding novel G protein-coupled receptors."
Marchese A., Docherty J.M., Nguyen T., Heiber M., Cheng R., Heng H.H.Q., Tsui L.-C., Shi X., George S.R., O'Dowd B.F.
Genomics 23:609-618(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-292.
[9]"Sphingosine 1-phosphate is a ligand of the human gpr3, gpr6 and gpr12 family of constitutively active G protein-coupled receptors."
Uhlenbrock K., Gassenhuber J., Kostenis E.
Cell. Signal. 14:941-953(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: PRELIMINARY FUNCTION.
[10]"Lipid G protein-coupled receptor ligand identification using beta-arrestin PathHunter assay."
Yin H., Chu A., Li W., Wang B., Shelton F., Otero F., Nguyen D.G., Caldwell J.S., Chen Y.A.
J. Biol. Chem. 284:12328-12338(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: SHOWS THAT IT IS NOT A SPHINGOSINE 1-PHOSPHATE RECEPTOR.
[11]"The orphan G protein-coupled receptor 3 modulates amyloid-beta peptide generation in neurons."
Thathiah A., Spittaels K., Hoffmann M., Staes M., Cohen A., Horre K., Vanbrabant M., Coun F., Baekelandt V., Delacourte A., Fischer D.F., Pollet D., De Strooper B., Merchiers P.
Science 323:946-951(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U18550 Genomic DNA. Translation: AAB60402.1.
L32831 Genomic DNA. Translation: AAA73560.1.
X83956 Genomic DNA. Translation: CAA58787.1.
AK291185 mRNA. Translation: BAF83874.1.
AK314868 mRNA. Translation: BAG37383.1.
AL096774 Genomic DNA. Translation: CAC18517.1.
CH471059 Genomic DNA. Translation: EAX07754.1.
BC032702 mRNA. Translation: AAH32702.1.
U13668 Genomic DNA. Translation: AAA64594.1.
PIRA55689.
RefSeqNP_005272.1. NM_005281.3.
UniGeneHs.66542.

3D structure databases

ProteinModelPortalP46089.
SMRP46089. Positions 39-308.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid109088. 4 interactions.
IntActP46089. 9 interactions.
STRING9606.ENSP00000363136.

Chemistry

GuidetoPHARMACOLOGY83.

Protein family/group databases

GPCRDBSearch...

Polymorphism databases

DMDM1170006.

Proteomic databases

PaxDbP46089.
PRIDEP46089.

Protocols and materials databases

DNASU2827.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000374024; ENSP00000363136; ENSG00000181773.
GeneID2827.
KEGGhsa:2827.
UCSCuc001bod.4. human.

Organism-specific databases

CTD2827.
GeneCardsGC01P027719.
H-InvDBHIX0160062.
HGNCHGNC:4484. GPR3.
HPACAB006254.
MIM600241. gene.
neXtProtNX_P46089.
PharmGKBPA28872.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG320944.
HOGENOMHOG000294075.
HOVERGENHBG079561.
InParanoidP46089.
KOK04311.
OMALWAICCC.
OrthoDBEOG7966H0.
PhylomeDBP46089.
TreeFamTF330052.

Gene expression databases

ArrayExpressP46089.
BgeeP46089.
CleanExHS_GPR3.
GenevestigatorP46089.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR000984. GPR3.
IPR000723. GPR_3/6/12_orphan.
[Graphical view]
PANTHERPTHR22750:SF9. PTHR22750:SF9. 1 hit.
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00237. GPCRRHODOPSN.
PR00648. GPR3ORPHANR.
PR00644. GPRORPHANR.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiGPR3.
GenomeRNAi2827.
NextBio11155.
PROP46089.
SOURCESearch...

Entry information

Entry nameGPR3_HUMAN
AccessionPrimary (citable) accession number: P46089
Secondary accession number(s): A8K570
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 16, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries