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P46086 (KIME_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mevalonate kinase
Short name=MK
EC=2.7.1.36
Gene names
Ordered Locus Names:At5g27450
ORF Names:F21A20.160
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length378 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + (R)-mevalonate = ADP + (R)-5-phosphomevalonate.

Enzyme regulation

Its activity is inhibited in vitro by geranyl pyrophosphate (GPP) and farnesyl pyrophosphate (FPP) that bind competitively at the ATP-binding site on the enzyme.

Pathway

Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 1/3.

Subcellular location

Cytoplasm Potential.

Sequence similarities

Belongs to the GHMP kinase family. Mevalonate kinase subfamily.

Sequence caution

The sequence AAD45421.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 378378Mevalonate kinase
PRO_0000156660

Regions

Nucleotide binding141 – 15111ATP By similarity

Sites

Binding site1381ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P46086 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 87E7E5C78BE53C66

FASTA37840,644
        10         20         30         40         50         60 
MEVKARAPGK IILAGEHAVV HGSTAVAAAI DLYTYVTLRF PLPSAENNDR LTLQLKDISL 

        70         80         90        100        110        120 
EFSWSLARIK EAIPYDSSTL CRSTPASCSE ETLKSIAVLV EEQNLPKEKM WLSSGISTFL 

       130        140        150        160        170        180 
WLYTRIIGFN PATVVINSEL PYGSGLGSSA ALCVALTAAL LASSISEKTR GNGWSSLDET 

       190        200        210        220        230        240 
NLELLNKWAF EGEKIIHGKP SGIDNTVSAY GNMIKFCSGE ITRLQSNMPL RMLITNTRVG 

       250        260        270        280        290        300 
RNTKALVSGV SQRAVRHPDA MKSVFNAVDS ISKELAAIIQ SKDETSVTEK EERIKELMEM 

       310        320        330        340        350        360 
NQGLLLSMGV SHSSIEAVIL TTVKHKLVSK LTGAGGGGCV LTLLPTGTVV DKVVEELESS 

       370 
GFQCFTALIG GNGAQICY

« Hide

References

« Hide 'large scale' references
[1]"Isolation and characterization of a cDNA encoding Arabidopsis thaliana mevalonate kinase by genetic complementation in yeast."
Riou C., Tourte Y., Lacroute F., Karst F.
Gene 148:293-297(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Landsberg erecta.
Tissue: Leaf.
[2]"Molecular cloning and expression analysis of the mevalonate kinase gene from Arabidopsis thaliana."
Lluch M.A., Masferrer A., Arro M., Boronat A., Ferrer A.
Plant Mol. Biol. 42:365-376(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. expand/collapse author list , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X77793 mRNA. Translation: CAA54820.1.
AF141853 Genomic DNA. Translation: AAD31719.1.
L77688 Genomic DNA. Translation: AAD45421.1. Sequence problems.
AC007123 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED93688.1.
CP002688 Genomic DNA. Translation: AED93689.1.
CP002688 Genomic DNA. Translation: AED93690.1.
AY084333 mRNA. Translation: AAM60916.1.
AY093165 mRNA. Translation: AAM13164.1.
BT002104 mRNA. Translation: AAN72115.1.
IPIIPI00533621.
PIRS42088.
RefSeqNP_001190411.1. NM_001203482.1.
NP_198097.1. NM_122627.5.
NP_851084.1. NM_180753.3.
UniGeneAt.30805.
At.5392.

3D structure databases

ProteinModelPortalP46086.
SMRP46086. Positions 7-376.
ModBaseSearch...

Protein-protein interaction databases

STRING3702.AT5G27450.1-P.

Proteomic databases

PaxDbP46086.
PRIDEP46086.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G27450.1; AT5G27450.1; AT5G27450.
AT5G27450.2; AT5G27450.2; AT5G27450.
AT5G27450.3; AT5G27450.3; AT5G27450.
GeneID832804.
KEGGath:AT5G27450.

Organism-specific databases

TAIRAt5g27450.

Phylogenomic databases

eggNOGCOG1577.
HOGENOMHOG000188934.
InParanoidP46086.
KOK00869.
OMAISCTCEK.
PhylomeDBP46086.
ProtClustDBPLN02677.

Enzyme and pathway databases

BioCycMetaCyc:AT5G27450-MONOMER.
UniPathwayUPA00057; UER00098.

Gene expression databases

GenevestigatorP46086.
GermOnlineAT5G27450. Arabidopsis thaliana.

Family and domain databases

Gene3D3.30.230.10. 1 hit.
InterProIPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR006203. GHMP_knse_ATP-bd_CS.
IPR006205. Mev_gal_kin.
IPR006206. Mevalonate/galactokinase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERPTHR10457:SF4. PTHR10457:SF4. 1 hit.
PfamPF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PRINTSPR00959. MEVGALKINASE.
SUPFAMSSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMsTIGR00549. mevalon_kin. 1 hit.
PROSITEPS00627. GHMP_KINASES_ATP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKIME_ARATH
AccessionPrimary (citable) accession number: P46086
Secondary accession number(s): Q9STB1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 1, 2013
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents