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Protein

Mevalonate kinase

Gene

At5g27450

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + (R)-mevalonate = ADP + (R)-5-phosphomevalonate.

Enzyme regulationi

Its activity is inhibited in vitro by geranyl pyrophosphate (GPP) and farnesyl pyrophosphate (FPP) that bind competitively at the ATP-binding site on the enzyme.

Pathwayi: isopentenyl diphosphate biosynthesis via mevalonate pathway

This protein is involved in step 1 of the subpathway that synthesizes isopentenyl diphosphate from (R)-mevalonate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Mevalonate kinase (At5g27450), Mevalonate kinase (AXX17_At5g27360)
  2. Phosphomevalonate kinase, peroxisomal (PMK)
  3. Diphosphomevalonate decarboxylase MVD1, peroxisomal (MVD1), Diphosphomevalonate decarboxylase MVD2, peroxisomal (MVD2)
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via mevalonate pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from (R)-mevalonate, the pathway isopentenyl diphosphate biosynthesis via mevalonate pathway and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei138ATPBy similarity1
Active sitei204Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi141 – 151ATPBy similarityAdd BLAST11

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processLipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:AT5G27450-MONOMER
ReactomeiR-ATH-191273 Cholesterol biosynthesis
UniPathwayiUPA00057; UER00098

Names & Taxonomyi

Protein namesi
Recommended name:
Mevalonate kinase (EC:2.7.1.36)
Short name:
MK
Gene namesi
Ordered Locus Names:At5g27450
ORF Names:F21A20.160
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G27450
TAIRilocus:2146375 AT5G27450

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001566601 – 378Mevalonate kinaseAdd BLAST378

Proteomic databases

PaxDbiP46086

Expressioni

Gene expression databases

ExpressionAtlasiP46086 baseline and differential
GenevisibleiP46086 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G27450.1

Structurei

3D structure databases

ProteinModelPortaliP46086
SMRiP46086
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1511 Eukaryota
COG1577 LUCA
HOGENOMiHOG000188934
InParanoidiP46086
KOiK00869
OMAiEGWKFWR
OrthoDBiEOG09360E6C
PhylomeDBiP46086

Family and domain databases

Gene3Di3.30.230.10, 2 hits
3.30.70.890, 1 hit
InterProiView protein in InterPro
IPR013750 GHMP_kinase_C_dom
IPR036554 GHMP_kinase_C_sf
IPR006204 GHMP_kinase_N_dom
IPR006203 GHMP_knse_ATP-bd_CS
IPR006205 Mev_gal_kin
IPR020568 Ribosomal_S5_D2-typ_fold
IPR014721 Ribosomal_S5_D2-typ_fold_subgr
PfamiView protein in Pfam
PF08544 GHMP_kinases_C, 1 hit
PF00288 GHMP_kinases_N, 1 hit
SUPFAMiSSF54211 SSF54211, 1 hit
SSF55060 SSF55060, 1 hit
TIGRFAMsiTIGR00549 mevalon_kin, 1 hit
PROSITEiView protein in PROSITE
PS00627 GHMP_KINASES_ATP, 1 hit

Sequencei

Sequence statusi: Complete.

P46086-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVKARAPGK IILAGEHAVV HGSTAVAAAI DLYTYVTLRF PLPSAENNDR
60 70 80 90 100
LTLQLKDISL EFSWSLARIK EAIPYDSSTL CRSTPASCSE ETLKSIAVLV
110 120 130 140 150
EEQNLPKEKM WLSSGISTFL WLYTRIIGFN PATVVINSEL PYGSGLGSSA
160 170 180 190 200
ALCVALTAAL LASSISEKTR GNGWSSLDET NLELLNKWAF EGEKIIHGKP
210 220 230 240 250
SGIDNTVSAY GNMIKFCSGE ITRLQSNMPL RMLITNTRVG RNTKALVSGV
260 270 280 290 300
SQRAVRHPDA MKSVFNAVDS ISKELAAIIQ SKDETSVTEK EERIKELMEM
310 320 330 340 350
NQGLLLSMGV SHSSIEAVIL TTVKHKLVSK LTGAGGGGCV LTLLPTGTVV
360 370
DKVVEELESS GFQCFTALIG GNGAQICY
Length:378
Mass (Da):40,644
Last modified:November 1, 1995 - v1
Checksum:i87E7E5C78BE53C66
GO

Sequence cautioni

The sequence AAD45421 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77793 mRNA Translation: CAA54820.1
AF141853 Genomic DNA Translation: AAD31719.1
L77688 Genomic DNA Translation: AAD45421.1 Sequence problems.
AC007123 Genomic DNA No translation available.
CP002688 Genomic DNA Translation: AED93688.1
CP002688 Genomic DNA Translation: AED93689.1
CP002688 Genomic DNA Translation: AED93690.1
AY084333 mRNA Translation: AAM60916.1
AY093165 mRNA Translation: AAM13164.1
BT002104 mRNA Translation: AAN72115.1
PIRiS42088
RefSeqiNP_001190411.1, NM_001203482.1
NP_198097.1, NM_122627.6
NP_851084.1, NM_180753.4
UniGeneiAt.30805
At.5392

Genome annotation databases

EnsemblPlantsiAT5G27450.1; AT5G27450.1; AT5G27450
AT5G27450.2; AT5G27450.2; AT5G27450
AT5G27450.3; AT5G27450.3; AT5G27450
GeneIDi832804
GrameneiAT5G27450.1; AT5G27450.1; AT5G27450
AT5G27450.2; AT5G27450.2; AT5G27450
AT5G27450.3; AT5G27450.3; AT5G27450
KEGGiath:AT5G27450

Similar proteinsi

Entry informationi

Entry nameiKIME_ARATH
AccessioniPrimary (citable) accession number: P46086
Secondary accession number(s): Q9STB1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 25, 2018
This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health